ObjectiveThis paper aims to explore the risk factors for chronic atrophic gastritis （CAG） with turbidity toxin accumulation syndrome and establish a prediction model. MethodsClinical data of 180 patients with CAG who participated in the "clinical study of Xianglian Huazhuo Particles blocking CAG cancer transformation" of Hebei Sheng Zhong Yi Yuan from July 2021 to March 2022 were collected. After confounding factors were controlled by propensity score matching， patients were divided into a training set （namely dev） and a validation set （namely vad） in a seven to three ratio. The risk factors for CAG with turbidity toxin accumulation syndrome in the training set were investigated by using univariate Logistic regression analysis and least absolute shrinkage and selection operator （namely Lasso） regression algorithms. Subsequently， a model， named model 1se， was developed by using the training set data to predict the risk factors for CAG with turbidity toxin accumulation syndrome. The accuracy of the prediction model was assessed by using various methods， including the receiver operating characteristic （ROC） curve， Hosmer-Lemeshow test （H-L）， calibration plot， and decision curve analysis （DCA）. ResultsAge， body mass index （BMI）， family history of cancer， job and life satisfaction， yellow and greasy fur with slippery pulse， and heavy body sensation were independent risk factors of the model. The prediction model showed excellent predictive value for both the training and validation sets. ConclusionThe established prediction model for CAG with turbidity toxin accumulation syndrome has high discrimination and excellent calibration， which could provide an excellent clinical basis for disease diagnosis and individualized treatment of patients.

ObjectiveThis paper aims to explore the risk factors for chronic atrophic gastritis （CAG） with turbidity toxin accumulation syndrome and establish a prediction model. MethodsClinical data of 180 patients with CAG who participated in the "clinical study of Xianglian Huazhuo Particles blocking CAG cancer transformation" of Hebei Sheng Zhong Yi Yuan from July 2021 to March 2022 were collected. After confounding factors were controlled by propensity score matching， patients were divided into a training set （namely dev） and a validation set （namely vad） in a seven to three ratio. The risk factors for CAG with turbidity toxin accumulation syndrome in the training set were investigated by using univariate Logistic regression analysis and least absolute shrinkage and selection operator （namely Lasso） regression algorithms. Subsequently， a model， named model 1se， was developed by using the training set data to predict the risk factors for CAG with turbidity toxin accumulation syndrome. The accuracy of the prediction model was assessed by using various methods， including the receiver operating characteristic （ROC） curve， Hosmer-Lemeshow test （H-L）， calibration plot， and decision curve analysis （DCA）. ResultsAge， body mass index （BMI）， family history of cancer， job and life satisfaction， yellow and greasy fur with slippery pulse， and heavy body sensation were independent risk factors of the model. The prediction model showed excellent predictive value for both the training and validation sets. ConclusionThe established prediction model for CAG with turbidity toxin accumulation syndrome has high discrimination and excellent calibration， which could provide an excellent clinical basis for disease diagnosis and individualized treatment of patients.

The infection incidence rate of Candida albicans,an opportunistic pathogenic fungus,has increased each year.Currently,the increased number of patients with immunodeficiency,the limited number of antifungal drugs,and drug resistance are the main reasons for the high incidence and high mortality of C.albicans related diseases.Therefore alternative therapies for combating C.albicans infections are urgently needed.Many recent studies have suggested that probiotics might inhibit C.albicans infection both directly,through inhibiting adhesion,growth,and biofilm formation,and indirectly,through regulating the host immune response,without leading to drug resistance.Because of the multiple pathways involved in these effects,the mechanism of probiotics in combating C.albicans infection has not been fully elucidated,and systematic evaluations of this mechanism are lacking.This article provides an in-depth analysis of the mechanism of probiotics in combating C.albicans infection described in recent years,to contribute to the development of new methods to resist C.albicans infection and,more importantly,the clinical development of new therapies for the treatment of drug-resistant fungal infections.

Under the background of new round of development in national and regional economies, the existing biomedical talent training system in local colleges is not matched in terms of education concept, specialty setting and course structure, which leads to its lack of openness and integration to strategic emerging industries and weak support. It calls for adjustments to the strategies for talent cultivation in local application-oriented colleges. On the one hand, they should optimize the structure of teaching and management team, look for new combination points and expand professional scope, combine interdisciplinary courses and improve the curriculum system. On the other hand, the entity platform integrating production, teaching and research should be explored, and innovation and entrepreneurship education should be guided to the in-depth development.

Objective To study the correlation and diagnostic significance of the silent information regulator 2 related enzyme 1(SIRT1)/nuclear factor kappa B(NF-κB)signaling pathway and cognitive impairment(CI)in patients with Parkinson's disease(PD).Methods A total of 119 patients with primary PD diagnosed and treated be in Taizhou Second People's Hospital from September 2017 to March 2021 were selected as PD group.According to cognitive function status of subjects,research subjects were divided into normal cognitive(NC)group(n=33),mild cognitive impairment(MCI)group(n=58),and dementia subgroup(PDD)group(n=28).In addition,50 healthy people for physical examination during the same period were selected as normal control group.After admission,the peripheral blood levels of SIRT1/NF-κB signaling pathway related molecules in all subjects were detected,and relationship between expression of SIRT1/NF-κB signaling pathway and cognitive function in PD patients was analyzed by partial correlation analysis.Cox proportional hazards regression model was used to analyze the influencing factors,and receiver operating characteristic(ROC)curve was used to evaluate the diagnostic efficacy of the SIRT1/NF-κB signaling pathway.Results SIRT1 mRNA expression in PD group was significantly lower than normal control group,while NF-κB mRNA expression was higher than normal control group(P＜0.05).PD group was further stratified and compared,and it was found that SIRT1 mRNA expression of PDD patients was significantly lower than that of NC and MCI patients(P＜0.05).SIRT1 mRNA and NF-κB mRNA had significant correlation with MoCA score(r=0.637,-0.527,P＜0.05).Cox regression analysis showed that high H-Y staging and high expression of NF-κB mRNA were independent risk factors for occurrence of CI in PD patients,and high expression of SIRT1 mRNA and high education level were independent protective factors for PD patients.ROC curve analysis showed that SIRT 1 and NF-κB mRNA expression diagnosed secondary CI in PD patients,and both had good predictive effects(P＜0.05).Conclusion SIRT1 mRNA and NF-κB mRNA were highly expressed in the peripheral blood of patients with PD secondary to CI,and had strong correlation with cognitive function.Early enhancement of the detection of SIRT1 mRNA and NF-κB mRNA expression in peripheral blood of PD patients can play an important role in diagnosis and prognosis.

The infection incidence rate of Candida albicans,an opportunistic pathogenic fungus,has increased each year.Currently,the increased number of patients with immunodeficiency,the limited number of antifungal drugs,and drug resistance are the main reasons for the high incidence and high mortality of C.albicans related diseases.Therefore alternative therapies for combating C.albicans infections are urgently needed.Many recent studies have suggested that probiotics might inhibit C.albicans infection both directly,through inhibiting adhesion,growth,and biofilm formation,and indirectly,through regulating the host immune response,without leading to drug resistance.Because of the multiple pathways involved in these effects,the mechanism of probiotics in combating C.albicans infection has not been fully elucidated,and systematic evaluations of this mechanism are lacking.This article provides an in-depth analysis of the mechanism of probiotics in combating C.albicans infection described in recent years,to contribute to the development of new methods to resist C.albicans infection and,more importantly,the clinical development of new therapies for the treatment of drug-resistant fungal infections.

Aim Systematic evolution of ligands by exponential enrichment(SELEX)techniquewas employed to screen and identify nucleic acid aptamers that specifically bind to mouse atherosclerotic pathological tissues,aiming to pro-vide a research foundation for the development of molecular targets and diagnostic reagents for early atherosclerosis.Methods A single-stranded DNA(ssDNA)library with a capacity of 1015～1016 was constructed,which was then subjec-ted to binding-elution(negative selection)with normal mouse vascular tissue slices.The eluted library was subsequently bound to atherosclerotic tissue slices for binding-elution(positive selection).PCR was used to amplify the positive and negative screening products,and agarose gel electrophoresis was used to verify the amplified products.The ssDNA library after multiple rounds of selection was sequenced using T-A cloning and sequencing to obtain the primary structure of the nu-cleic acid aptamers,and the secondary structure was predicted using the Mfold online software.The selected nucleic acid aptamers were labeled with a FAM fluorescent group at the 5'-end and were bound to both positive and negative selection tissue slices,with fluorescence intensity observed under a fluorescence microscope.Image Pro Plus 6.0 was used to cal-culate the relative average fluorescence intensity to evaluate the binding specificity of nucleic acid aptamers.Results After 8 rounds of selection,agarose gel electrophoresis imaging showed PCR amplification products in the positive selection lanes,while no PCR amplification products were observed in the negative selection lanes,indicating the successful acquisi-tion of a nucleic acid aptamer library that specifically binds to atherosclerotic tissues.Five nucleic acid aptamers were i-dentified by T-A cloning and sequencing,and their predicted secondary structures all had stem-loop structures.Immuno-fluorescence staining verified that five nucleic acid aptamers had different degrees of binding with As blood vessels,and the quantitative results of the relative average fluorescence intensity showed that nucleic acid aptamer No.11 had the highest relative average fluorescence intensity value,which can be used as a candidate nucleic acid aptamer for subsequent re-search.Conclusion Specific nucleic acid aptamers that bind to atherosclerotic vesselswere successfully obtained,providing a research foundation for further screening of early molecular targets of Asand developing in vivo early diagnostic reagents.

Objective To evaluate the effectiveness of the PDCA cycle in critical value management.Methods PDCA cycle method was applied to manage critical values in an occupational disease hospital.Critical value data from October 2021 to March 2022 were analyzed to identify root causes,followed by corrective measures development and implementation.Post-inter-vention data,from July 2022 to November 2022,were compared with the pre-management data to evaluate the implementation effect of the PDCA cycle on critical value management.Results The application of the PDCA cycle led to a statistically signifi-cant increase in the execution compliance rate for emergency value execution in medical and technical departments increased to 100.00％,and in the clinical departments to 96.77％(P＜0.05).Conclusion PDCA cycle can improve the qualified rate of the implementation of critical value system,ensure patients safety,significantly reduce medical risks,and advance the level of standardization and precision in critical value management within our hospital.

Aim Systematic evolution of ligands by exponential enrichment(SELEX)techniquewas employed to screen and identify nucleic acid aptamers that specifically bind to mouse atherosclerotic pathological tissues,aiming to pro-vide a research foundation for the development of molecular targets and diagnostic reagents for early atherosclerosis.Methods A single-stranded DNA(ssDNA)library with a capacity of 1015～1016 was constructed,which was then subjec-ted to binding-elution(negative selection)with normal mouse vascular tissue slices.The eluted library was subsequently bound to atherosclerotic tissue slices for binding-elution(positive selection).PCR was used to amplify the positive and negative screening products,and agarose gel electrophoresis was used to verify the amplified products.The ssDNA library after multiple rounds of selection was sequenced using T-A cloning and sequencing to obtain the primary structure of the nu-cleic acid aptamers,and the secondary structure was predicted using the Mfold online software.The selected nucleic acid aptamers were labeled with a FAM fluorescent group at the 5'-end and were bound to both positive and negative selection tissue slices,with fluorescence intensity observed under a fluorescence microscope.Image Pro Plus 6.0 was used to cal-culate the relative average fluorescence intensity to evaluate the binding specificity of nucleic acid aptamers.Results After 8 rounds of selection,agarose gel electrophoresis imaging showed PCR amplification products in the positive selection lanes,while no PCR amplification products were observed in the negative selection lanes,indicating the successful acquisi-tion of a nucleic acid aptamer library that specifically binds to atherosclerotic tissues.Five nucleic acid aptamers were i-dentified by T-A cloning and sequencing,and their predicted secondary structures all had stem-loop structures.Immuno-fluorescence staining verified that five nucleic acid aptamers had different degrees of binding with As blood vessels,and the quantitative results of the relative average fluorescence intensity showed that nucleic acid aptamer No.11 had the highest relative average fluorescence intensity value,which can be used as a candidate nucleic acid aptamer for subsequent re-search.Conclusion Specific nucleic acid aptamers that bind to atherosclerotic vesselswere successfully obtained,providing a research foundation for further screening of early molecular targets of Asand developing in vivo early diagnostic reagents.

Under the background of new round of development in national and regional economies, the existing biomedical talent training system in local colleges is not matched in terms of education concept, specialty setting and course structure, which leads to its lack of openness and integration to strategic emerging industries and weak support. It calls for adjustments to the strategies for talent cultivation in local application-oriented colleges. On the one hand, they should optimize the structure of teaching and management team, look for new combination points and expand professional scope, combine interdisciplinary courses and improve the curriculum system. On the other hand, the entity platform integrating production, teaching and research should be explored, and innovation and entrepreneurship education should be guided to the in-depth development.