By critically examining studies ranging from randomized controlled trials to meta-analyses and cross- sectional studies, we seek to elucidate the potential benefits of vitamin C (vit. C) supplementation in mitigating oxidative stress associated with cannabis (CS) consumption. Twenty female rats with mean weight of (160 g ± 1.09) were separately assigned into four groups of five animals each. The rats in groups 1, 2, 3 and 4 respectively received orally 1.0 ml of distilled water (control), 2.0mg/kg body weight (bw) CS+4mg/kg bw vit. C, 4.0mg/kgbw CS+4.0mg/kg bw vit. C and 6.0mg/kgbw CS+mg/4.0kg bw vit. C respectively for two weeks. All the groups have free access to food and water. All the rats were sacrificed on the 15th day. Lactate dehydrogenase (LDH), Catalase, Glutathione reductase (GSH), Glutathione peroxide (GPx), Malondialdehyde (MDA) and Total Antioxidant Capacity (TAC) were determined using standard methods.

Smoking of Cannabis sativa (Marijuana), a known psychoactive substance may result in side effect on living cells of the system. The aim of this study was to investigate the effect of Cannabis sativa (CS) on oxidative stress and the recovery period in male rats. Forty rats with mean weight of (170 g ± 1.24) were separately assigned into four groups of ten animals each. The rats in groups 1, 2, 3 and 4 received orally 1.0 ml of distilled water (control), 2.0mg, 4.0mg and 6.0mg of CS respectively for two weeks. Five animals from each group were sacrificed on the 15th day and the remaining animals were left for additional two weeks without treatment but with access to water and food before sacrifice.