Objective: To analyze the screening results and influencing factors of active pulmonary tuberculosis (PTB) and latent tuberculosis infection (LTBI) among close contacts of PTB patients in Sichuan Province, so as to provide the evidence for optimizing hierarchical management strategies and promoting preventive treatment. Methods: Data of newly registered active PTB patients in Sichuan Province in 2025 were extracted from the Tuberculosis Management Information System of China Disease Prevention and Control Information System. Information on close contacts of PTB patients was collected using the 2025 Individual Screening Registration Form for Close Contacts of Pulmonary Tuberculosis Patients. Active pulmonary tuberculosis was diagnosed according to WS 288—2017 Diagnosis of Pulmonary Tuberculosis. Individuals with positive Mycobacterium tuberculosis infection who were clinically evaluated to have excluded active pulmonary tuberculosis were classified as having LTBI. Multivariable logistic regression models were applied to identify influencing factors for active PTB and LTBI among close contacts. Results: A total of 25 451 newly registered PTB patients and 65 459 corresponding close contacts were recorded in Sichuan Province in 2025, with a contact-to-patient registration ratio of 2.57:1. Among all close contacts, 28 902 (44.15%) were males and 36 557 (55.85%) were females. The age group of 41-<61 years accounted for the largest proportion (22 856, 34.92%). Multivariable logistic regression revealed that close contacts aged 41-<61 years had a lower risk of developing active PTB (OR=0.721, 95%CI: 0.574-0.905). Higher risks of active PTB were observed among contacts with household exposure (OR=1.274, 95%CI: 1.012-1.604), suspicious TB symptoms (OR=12.979, 95%CI: 9.361-17.996), and contacts of patients with positive etiological test results (OR=1.385, 95%CI: 1.071-1.791). For LTBI risk, elevated odds were found in contacts aged ≥21 years (21-<41 years, OR=1.627, 95%CI: 1.471-1.800; 41-<61 years, OR=2.113, 95%CI: 1.933-2.310; ≥61 years, OR=1.731, 95%CI: 1.573-1.905), those with household exposure (OR=1.092, 95%CI: 1.012-1.178), individuals presenting suspicious TB symptoms (OR=4.170, 95%CI: 3.326-5.229), and contacts of etiologically positive PTB patients (OR=1.403, 95%CI: 1.283-1.534). Conclusion Active PTB and LTBI among close contacts of pulmonary tuberculosis patients in Sichuan Province were associated with factors including age, type of exposure, presence of presumptive tuberculosis symptoms, and etiological results of the index patients.

Objective To detect the spatial clustering and high risk areas of pulmonary tuberculosis (PTB) in Sichuan province in 2018 and,to compare the effects of application on both SaTScan 9.4.1 software and FleXScan 3.1.2 software to detect the PTB spatial clusters.Methods Geographic information database was established by using the incidence data of PTB and demographic data reported in the'China disease prevention of infectious disease reporting information management system'in Sichuan province in 2018.Spatial clustering analysis was conducted using the Poisson model in software SaTScan 9.4.1 and FleXScan 3.1.2 to detect the high risk areas of PTB by software ArcGIS 10.5.Differences of clusters locations and scopes in the two scanning methods were compared.Results The reported incidence rate of PTB was 57.34/100 000 (47 601 cases) in Sichuan province in 2018,presenting an obvious clustering distribution.SaTScan and FleXScan scanned 8 and 10 clusters showed statistically significant differences (P＜0.05),with log-likelihood ratio (LLR) as 24.62-2 416.05 and 1.48-2 618.96,respectively.Results from scanning of the two methods showed that the most likely clusters appeared in the Daliangshan and Xiaoliangshan of Liangshan Yi ethnic aggregation areas.The other clustering areas would include some minority areas in the western Sichuan plateau,detected by both two methods differences in the shape and scope of the clustering were detected by both methods.The clustering scopes detected by SaTScan covered some counties,in which the actual incidence was not high.FleXScan could distinguish the clusters and detect more irregular shaped clusters.Conclusions Obvious spatial clusters of PTB distribution were found in Sichuan in 2018.Areas of Daliangshan,Xiaoliangshan and the minority areas in Western Sichuan plateau appeared at high risk,suggesting these were the key areas for prevention and control.FleXScan seemed more conducive in accurately distinguishing the "cold spot" areas in the highly aggregated areas,and more suitable for the application of spatial clustering detection for TB,in Sichuan province.

Objective To analyze the spatial and temporal distribution of smear positive pulmonary tuberculosis (PTB) in Liangshan Yi autonomous prefecture in Sichuan province from 2011 to 2016. Methods The registration data of PTB in 618 townships of Liangshan from 2011 to 2016 were collected from"Tuberculosis Management Information System of National Disease Prevention and Control Information System". Software ArcGIS 10.2 was used to establish the geographic information database and realize the visualization of the analysis results. Software OpenGeoda 1.2.0 was used to conduct the analyses on global indication of spatial autocorrelation (GISA) and local indication of spatial autocorrelation (LISA). Software SaTScan 9.4.1 was used for spatio-temporal scanning analysis. Results From 2011 to 2016, the registration rate of smear positive PTB in Liangshan declined from 56.97/100000 (2666 cases) to 21.11/100000 (1038 cases). The global spatial autocorrelation coefficient Moran's I ranged from 0.25 to 0.45 and the difference was significant (all P=0.000). Local autocorrelation analysis showed that"high-high"area covered 43, 34, 37, 34, 42 and 61 townships from 2011 to 2016, respectively, mainly in Leibo county. Spatial temporal clustering analysis found one class Ⅰ clustering in the area around Bagu township of Meigu county and two class Ⅱ clustering in the areas around Liumin and Hekou township of Huili county, respectively (all P=0.000). Conclusion Obvious spatial temporal clustering of smear positive PTB distribution was found in Liangshan from 2011-2016. Hot spot areas with serious smear positive PTB epidemic and high spread risk were mainly found in northeastern Liangshan, including townships in Leibo and Meigu counties. Targeted TB prevention and control should be conducted in these areas.

Objective To analyze systematically the risks and protective factors of lung cancer in female residents of Zhoushan,so as to provide evidence for early diagnosis and treatment of lung cancer.Methods A population-based case-control study involving interviews with 215 lung cancer female patients and 227 controls was carried out from October,2010 to October,2012.The subjects were interviewed and information about their exposure to related factors was surveyed with questionnaire.Statistical analysis by using single-factor and multivariate conditional Logistic regression was performed to determine causes and protective factors of lung cancer.Results Passive smoking index ≥ 50 man-years (OR =1.568,95％ CI =25.134-63.327),loving to eat pickled products (OR =4.778,95％ CI =3.462-784.219),frequent occupational exposure to dust (OR =3.543,95％ CI =4.003-115.014) and excessive exposure to kitchen smog (OR =8.113,95％CI =0.135-0.763) could increase the risk of lung cancer.On the other hand,lovingto take vegetables (OR =0.030,95％ CI =0.103-0.489),loving to eat eggs (OR =0.023,95％ CI =3.132-56.427),loving to take shellfish (OR =0.743,95％ CI =4.671-91.547),initial reproductive age between 20 and 30 (OR =0.032,95％ CI =4.127-10.736) and high satisfaction with family life (OR =0.009,95％ CI =0.581-8.169) were all protective factors against cancer risk.Conclusions Passive smoking,intake of pickled products,occupational exposure to dust and exposure to kitchen smog were all main risk factors for lung cancer among females in Zhoushan archipelago,while frequent intake of vegetables,seashells,eggs and high satisfaction with family life seemed to have protective effects on lung cancer.

Objective To analyze systematically the risks and protective factors of lung cancer in female residents of Zhoushan,so as to provide evidence for early diagnosis and treatment of lung cancer.Methods A population-based case-control study involving interviews with 215 lung cancer female patients and 227 controls was carried out from October,2010 to October,2012.The subjects were interviewed and information about their exposure to related factors was surveyed with questionnaire.Statistical analysis by using single-factor and multivariate conditional Logistic regression was performed to determine causes and protective factors of lung cancer.Results Passive smoking index ≥ 50 man-years (OR =1.568,95％ CI =25.134-63.327),loving to eat pickled products (OR =4.778,95％ CI =3.462-784.219),frequent occupational exposure to dust (OR =3.543,95％ CI =4.003-115.014) and excessive exposure to kitchen smog (OR =8.113,95％CI =0.135-0.763) could increase the risk of lung cancer.On the other hand,lovingto take vegetables (OR =0.030,95％ CI =0.103-0.489),loving to eat eggs (OR =0.023,95％ CI =3.132-56.427),loving to take shellfish (OR =0.743,95％ CI =4.671-91.547),initial reproductive age between 20 and 30 (OR =0.032,95％ CI =4.127-10.736) and high satisfaction with family life (OR =0.009,95％ CI =0.581-8.169) were all protective factors against cancer risk.Conclusions Passive smoking,intake of pickled products,occupational exposure to dust and exposure to kitchen smog were all main risk factors for lung cancer among females in Zhoushan archipelago,while frequent intake of vegetables,seashells,eggs and high satisfaction with family life seemed to have protective effects on lung cancer.

Objective To evaluate the clinical diagnostic application and operative efficacy of the expression of NKG2D in peripheral blood CD+8 NKT cell and its ligand sMICA in patients with esophageal or cardiac carcinoma.Methods The peripheral blood NKG2D positive CD+8 NKT cell percentage was concomitantly determined by flow cytometry in 53 preoperative patients including 29 postoperative patients with esophageal or cardiac carcinoma and 30 healthy controls.The serum sMICA was determined by ELISA.Results The peripheral blood NKG2D positive CD+8 NKT cell percentage in patients was significantly lower than that in controls [(77.632±8.972) % vs (89.053±6.515) %] (t = -6.113,P ＜0.05); with stage Ⅱ,Ⅲ,Ⅳ,it decreased significantly in order (F = 99.251,P ＜0.01);with lymph node metastasis lower than that without lymph node metastasis (t = -10.384,P ＜0.01); squamous carcinoma was higher than adenocarcinoma (t =9.899,P ＜0.01); postoperative was significantly higher than preoperative (t =-4.319,P ＜0.01).The level of serum sMICA in patients was significantly higher than that in controls [(326.28±85.407) pg/ml vs (210.00±92.560) pg/ml](t =7.292,P ＜0.01); with stage Ⅱ,Ⅲ,Ⅳ,it increased significautly in order (F =63.355,P ＜0.01); with lymph node metastasis higher than that without lymph node metastasis (t =7.770,P ＜0.01); squamous carcinoma was lower than adenocarcinoma (t =-7.593,P＜0.01); postoperative was significantly lower than preoperative (t =7.027,P ＜0.01).Serum sMICA could inhibit peripheral blood CD+8 NKT cell activation receptor NKG2D (F =142.773,P ＜0.05),determination coefficient R2 = 0.7368.Conclusion The level of peripheral blood CD+8NKT cell activation receptor NKG2D and serum sMICA in patients could be an assistant indicator for

Objective To study the influence of probiotic bacteria on rats blood lipids and immune function.Methods Rats fed with high fat diet,as a positive control of lovastatin in different kinds of experimental rats fed with probiotic bacteria.Before the experiment and 30 days after the experiment,the serum TC,TG and immune indicators were examined,compared with the control group.Results Probiotic bacteria feeding rats compared with control group rats,TC and TG in probiotic bacteria feeding rats blood are obviously lower than the eontroled group,(P＜0.01),the immune index is obviously higher than the control group (P＜0.05).Conclusion Probiotic bacteria obviously reduces TG and TG in Probiotic feeding rats blood,and improves the immunological function of probiotic bacteria feeding rats.

BACKGROUND:Contraction of three-dimensional collagen gels has been used as a model for the contraction which characterizes both normal wound healing and the development of fibrosis in the tissue. Several factors and cytokines,such as tumor necrosis factor alpha (TNF-α), interleukin-1, prostaglandin E2 and insulin have been proved to play important roles in collagen remodeling in vitro as well as serum extravasation during the fibrotic progress.OBJECTIVE: To observe extracellular collagen matrix contraction and apoptosis of fetal lung fibroblasts in TNF-α,interleukin-1, insulin, prostaglandin E2, albumin and globum under three-dimensional culture, and investigate the effects of cytokines, insuin, serum and serum protein on the remodeling and fibrotic formation of lung tissue.DESIGN: A randomized controlled experiment.SETTING: Department of Respiratory Medicine, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA.MATERIALS: The experiment was carried out The experiments were carried out in the respiratory laboratory of Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA from August 2005 to January 2006. Human fetal lung fibroblasts (American Type Culture Collection), Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum, insulin, transforming growth factor (TGF) (R&D), prostaglandin E2, type Ⅰ collagen was extracted from rat-tail tendons.METHODS: In order to investigate the effect of initial collagen concentration in the gels on the contractility of fibroblasts,the appropriate amount of collagen was mixed with distilled water, four fold-concentrated DMEM, and cells were suspended so that the mass concentration was 0.75-2.0 g/L, with a physiological ionic strength and the desired cell concentration. In order to investigate the effect of cell number in the gels on the contraction, the cellular concentration fibroblasts in the gels were prepared to 0.2×107-4×107 L-1. The areas of floating gels were measured daily and the contraction was calculated by contrasting the initial size (％ of initial area). Different serum concentrations (0.01％-0.5％)in the medium were prepared, the serum albumin (0.1％) or globulin (0.1％) were added to the serum-free culture medium to observe the gel contraction. TGF (10 mg/L), interleukin-1 (10 mg/L), insulin (1 mg/L) and prostaglandin E2 (0.1 μmol/L) were added to observe the effects of cytokines and insulin on fibroblasts-mediated collagen gel contraction.The DNA content and cellular survivability in gels in collagen were determined.MAIN OUTCOME MEASURES: Effect of lung fibroblasts on collagen contraction with or without the presence of cytokines in three-dimensional culture; Effect of collagen with different concentration on the proliferation and apoptosis.RESULTS: ① Collagen gel contraction showed a dependence on the number of fibroblasts. ② Collagen gel contraction was augmented by increasing serum concentration in culture medium, and albumin increased the contraction dramatically. ③TGF and insulin significantly increased the contraction, whereas prostaglandin E2 and interleukin-1 significantly inhibited the gel contraction. ④ The lower the initial collagen concentration was, the more gel contracted and smaller final size were observed, and cell apoptosis increased.CONCLUSION: During the fibrotic process, fibroblast population migrated into the injured lung tissue may play an important role in the development of pulmonary fibrosis and serum infiltrating into injury lung tissue may play an role in stimulating the fibrotic progress. Infiltrating fluids and edema result in the dilution of the collagen concentration in the pulmonary interstitial which may lead to stronger contraction and serious fibrosis. In the dense fibrosis area, cells were hard to survive. In consequence, the final structure of fibrotic lung could not been changed and lung fibrosis progressed.

Objective To investigate the protective and cure effects of dexamethasone on bleomycin-induced pulmonary fibrosis.Methods32 rats were randomly divided into the control group (C-group, n=8), injury group (I-group, n=12) and dexamethasone group (D-group, n=12). The acute pulmonary model was established by intratracheal injection of bleomycin with rats of the I-group and D-group; while rats of the C-group injected with distilled water. After that, rats of the D-group were injected with dexamethasone sodium phosphate in intraperitoneal every day, those of the C-group and I-group were injected with saline. The animals were killed on the 3rd, 7th, 14th, and 27th days after treatment, and tests of bronchoalveolar lavage fluid (BALF), total lung collagen content and lung tissue processing were performed.ResultsPathological evidence of the I-group rats demonstrated that the alveolar compartment companied with massive inflammatory cell invasion and a number of myofibroblast proliferation became more thick. However, lung injury in the D-group rats got better than that in the I-group. Neutrophil percentage achieved peak in both I-group and D-group on the 7th day. But the neutrophil ratio in the D-group was significantly lower than that of the I-group on the 7th day ( P<0.05) and the 14th day ( P<0.01). Total lung collagen content achieved peak on the 14th day both in I-group and D-group, but that of the I-group was significantly higher than that of the C-group ( P<0.01) and the D-group was significantly lower than the I-group ( P<0.01).ConclusionDexamethasone plays a protective and cure role in lung fibrosis by efficiently inhibiting the gather and invasion of neutropils and restraining the increase of collagen secreted by proliferous fibroblasts.

Objective To research the portable electron video equipment for tracheal intubatton in first aid. Methods According to the attributes of physiological bend and specialty of windpipe and gular of image, a horniness endoscopic of "C" model was designed with optical interface, CCD image transducer and small crystal display. It could quickly and visually lead tracheal intubatton through nonnasality. Results It was portable, visual, easily-operated. The rate of successful leading of tracheal intubatton is 97.8% . Conclusion It is especially suited to field battle, first aid, abrupt affairs, etc.