Objective Based on the connotation of high-quality development,it aims to establish an evaluation index system for assessing the qualification of public hospitals to expand bed capacity,providing a reference tool for evaluating suitable hospital scale.Methods A hospital bed resource usage database and indicator system were established based on policy reports and relevant studies.Multiple rounds of expert consultations were conducted to select indicators and set"red lines"to ensure scientific validity and practicality.Results An evaluation system was created with 4 dimensions and 12 indicators for existing hospital campus bed expansion,and 3 dimensions and 4 indicators for new sub-campus expansion.The feasibility of pilot testing was good.Conclusion The proposed indicators effectively explain the aspects of medical service quality and efficiency,sustainability,and patient satisfaction under the framework of high-quality development,offering solutions to address bed resource misallocation.

Objective Based on the connotation of high-quality development,it aims to establish an evaluation index system for assessing the qualification of public hospitals to expand bed capacity,providing a reference tool for evaluating suitable hospital scale.Methods A hospital bed resource usage database and indicator system were established based on policy reports and relevant studies.Multiple rounds of expert consultations were conducted to select indicators and set"red lines"to ensure scientific validity and practicality.Results An evaluation system was created with 4 dimensions and 12 indicators for existing hospital campus bed expansion,and 3 dimensions and 4 indicators for new sub-campus expansion.The feasibility of pilot testing was good.Conclusion The proposed indicators effectively explain the aspects of medical service quality and efficiency,sustainability,and patient satisfaction under the framework of high-quality development,offering solutions to address bed resource misallocation.

Objective To analyze the mechanisms of meaning in life and living arrangements impacting the effect of activities of dai-ly living(ADL)on depression for older adults.Methods From September to November,2024,community-dwelling older adults from Weifang,Shandong,were investi-gated with questionnaire using Basic Activities of Daily Living Scale(BADL),Meaning in Life Questionnaire(MLQ),Geriatric Depression Scale(GDS),and a general information questionnaire including living arrange-ments.Results A total of 256 questionnaires were distributed,and 245 valid questionnaires were returned.There were signifi-cant differences in GDS scores among older adults with different personal monthly incomes and living arrange-ments(F>3.744,P<0.05).BADL scores were positively correlated with GDS scores(r=0.490,P<0.001)and negatively correlated with MLQ scores(r=-0.370,P<0.001),and MLQ scores were negatively correlated with GDS scores(r=-0.369,P<0.001).There was significant direct effect of BADL scores on GDS scores(effect=1.128,95%CI 0.829～1.427),and mediating effect of MLQ scores on the relationship between BADL and GDS scores(effect=0.171,95%CI 0.028～0.337).The moderating effect of living alone was significant(|B|=0.127,|t|=2.103,P<0.05)on the association between MLQ and GDS,compared with intergenerational coresidence.Conclusion Meaning in life partially mediates the association between ADL and depression in older adults,especially for those living alone.

Objective To analyze the mechanisms of meaning in life and living arrangements impacting the effect of activities of dai-ly living(ADL)on depression for older adults.Methods From September to November,2024,community-dwelling older adults from Weifang,Shandong,were investi-gated with questionnaire using Basic Activities of Daily Living Scale(BADL),Meaning in Life Questionnaire(MLQ),Geriatric Depression Scale(GDS),and a general information questionnaire including living arrange-ments.Results A total of 256 questionnaires were distributed,and 245 valid questionnaires were returned.There were signifi-cant differences in GDS scores among older adults with different personal monthly incomes and living arrange-ments(F>3.744,P<0.05).BADL scores were positively correlated with GDS scores(r=0.490,P<0.001)and negatively correlated with MLQ scores(r=-0.370,P<0.001),and MLQ scores were negatively correlated with GDS scores(r=-0.369,P<0.001).There was significant direct effect of BADL scores on GDS scores(effect=1.128,95%CI 0.829～1.427),and mediating effect of MLQ scores on the relationship between BADL and GDS scores(effect=0.171,95%CI 0.028～0.337).The moderating effect of living alone was significant(|B|=0.127,|t|=2.103,P<0.05)on the association between MLQ and GDS,compared with intergenerational coresidence.Conclusion Meaning in life partially mediates the association between ADL and depression in older adults,especially for those living alone.

Objective To compare ferroptosis characteristics and motor dysfunction across different mouse models of Parkinson's disease(PD).Methods Animal models of PD were divided into five groups:the control group,sham-operated group,6-hydroxydopamine(6-OHDA)group,1-methyl-4-phenyl-1,2,3,6-tetrahydropyri-dine(MPTP)group,and lipopolysaccharide(LPS)group.Differences in ferroptosis-related features and motor dysfunction across these groups were evaluated through behavioral observation,histopathological examination,protein analysis,and assessment of lipid peroxidation and oxidative stress levels.Results In all three model groups,the number of TH-positive neurons in the substantia nigra on the lesioned side was significantly reduced,accompanied by evident neuronal degeneration and a marked decrease in Nissl bodies.Behavioral assessments revealed that the 6-OHDA group displayed the most severe motor deficits.In the substantia nigra,FTH1 protein expression was significantly downregulated in the 6-OHDA,LPS,and MPTP groups(P<0.01,P<0.05,P<0.01),respectively,while GPX4 expression was also reduced(P<0.001,P<0.001,P<0.01).Malondialdehyde(MDA)levels were significantly elevated in both the 6-OHDA and MPTP groups(P<0.05,P<0.05).Glutathione(GSH)assays demonstrated markedly reduced levels in the 6-OHDA,MPTP,and LPS groups(P<0.01,P<0.001,P<0.001).Reactive oxygen species(ROS)detection revealed a significant increase in the MPTP and LPS groups(P<0.05,P<0.05).Conclusion The 6-OHDA model is well suited for studying PD-related behaviors and the underlying mechanisms of motor symptoms,the MPTP model is particularly effective for investigating the ferroptosis pathway,and the LPS model serves as a valuable complement for research on neuroinflammation mechanisms.

Anxiety disorder is a common emotional disorder.Ophthalmic diseases, which can cause ocular pain, discomfort, and irreversible visual impairment, not only lead to a deterioration in patients' quality of life but also give rise to mental health issues, including anxiety and depression.Numerous studies have indicated that a substantial number of patients with eye diseases frequently exhibit anxiety symptoms, and the prevalence rate is notably higher compared to that of the general population.This relationship is bidirectional.On the one hand, factors associated with eye diseases, such as vision loss, changes in appearance, economic burden, and reduced quality of life, can trigger or exacerbate anxiety.On the other hand, anxiety can accelerate the onset and progression of certain eye diseases, such as dry eye, pseudo-myopia and glaucoma by influencing neurotransmitter function, the autonomic nervous system, and the hypothalamus-pituitary-adrenal axis.The alleviation of eye conditions necessitates not only active treatment of the disease itself, but also maintaining a healthy psychological state.This review reviews the reciprocal relationship between various eye diseases, including dry eye, glaucoma, diabetic retinopathy, and age-related macular degeneration, and anxiety to more clearly elucidate their mutual influence, provide guidance for integrated psychological treatment in the clinical management of eye diseases and raise ophthalmologists' awareness of the significance of mental health in disease treatment.

Objective To explore the expression level,clinical significance of protein disulfide isomerase A3(PDIA3)in endome-trial cancer(EC)tissues,and its effects on Ishikawa cells.Methods Using the TCGA database to analyze the differential expression levels of PDIA3 in different endometrial tissues and explore its relationship with clinical pathological parameters and prognosis of EC pa-tients;Using immunohistochemistry(IHC)to detect the expression level of PDIA3 in clinical endometrial tissue,analyze the clinical pathological parameters,prognosis,and risk factors affecting the prognosis of EC patients with different expression levels;Using Transwell assay,scratch assay,and CCK-8 assay to detect the proliferation,invasion,and migration ability of Ishikawa cells with knockdown PDIA3 expression.Results The expression level of PDIA3 in EC tissue was higher than that in normal endometrial tissue in the TCGA database and clinical specimens(P＜0.05).The expression level of PDIA3 in EC tissue in the G3 grade group was higher than that in the G1 and G2 grade groups in the TCGA database(P＜0.05).The expression level of PDIA3 in EC tissue in FIGO stage Ⅱ-Ⅳ group was higher than that in the stage Ⅰ group,the group with lymph node metastasis was higher than that without lymph node metastasis,and the highly differentiated group was higher than the poorly differentiated group in clinical specimens(P＜0.05);COX regression analysis showed that Federation International of Gynecology and Obstetrics(FIGO)staging and PDIA3 expression level were the independent risk factors of prognosis in EC patients;the survival time of EC patients with high expression of PDIA3 in the TCGA database was shorter than that of the low expression group(P＜0.05),and the survival rate of clinical patients with positive PDIA3 was shorter than that of the neg-ative group(P＜0.05);Transwell assay,scratch assay,and CCK-8 assay showed that the invasion and migration ability of cells in the PDIA3 inhibition group was significantly reduced compared to the normal group and negative control group(P＜0.05).Conclusion PDIA3 is highly expressed in EC tissues and is associated with the malignancy of tumors and poor prognosis of patients;targeted inhibition of PDIA3 expression can reduce the proliferation,invasion,and migration ability of Ishikawa cells.

Anxiety disorder is a common emotional disorder.Ophthalmic diseases, which can cause ocular pain, discomfort, and irreversible visual impairment, not only lead to a deterioration in patients' quality of life but also give rise to mental health issues, including anxiety and depression.Numerous studies have indicated that a substantial number of patients with eye diseases frequently exhibit anxiety symptoms, and the prevalence rate is notably higher compared to that of the general population.This relationship is bidirectional.On the one hand, factors associated with eye diseases, such as vision loss, changes in appearance, economic burden, and reduced quality of life, can trigger or exacerbate anxiety.On the other hand, anxiety can accelerate the onset and progression of certain eye diseases, such as dry eye, pseudo-myopia and glaucoma by influencing neurotransmitter function, the autonomic nervous system, and the hypothalamus-pituitary-adrenal axis.The alleviation of eye conditions necessitates not only active treatment of the disease itself, but also maintaining a healthy psychological state.This review reviews the reciprocal relationship between various eye diseases, including dry eye, glaucoma, diabetic retinopathy, and age-related macular degeneration, and anxiety to more clearly elucidate their mutual influence, provide guidance for integrated psychological treatment in the clinical management of eye diseases and raise ophthalmologists' awareness of the significance of mental health in disease treatment.

Objective To explore the expression level,clinical significance of protein disulfide isomerase A3(PDIA3)in endome-trial cancer(EC)tissues,and its effects on Ishikawa cells.Methods Using the TCGA database to analyze the differential expression levels of PDIA3 in different endometrial tissues and explore its relationship with clinical pathological parameters and prognosis of EC pa-tients;Using immunohistochemistry(IHC)to detect the expression level of PDIA3 in clinical endometrial tissue,analyze the clinical pathological parameters,prognosis,and risk factors affecting the prognosis of EC patients with different expression levels;Using Transwell assay,scratch assay,and CCK-8 assay to detect the proliferation,invasion,and migration ability of Ishikawa cells with knockdown PDIA3 expression.Results The expression level of PDIA3 in EC tissue was higher than that in normal endometrial tissue in the TCGA database and clinical specimens(P＜0.05).The expression level of PDIA3 in EC tissue in the G3 grade group was higher than that in the G1 and G2 grade groups in the TCGA database(P＜0.05).The expression level of PDIA3 in EC tissue in FIGO stage Ⅱ-Ⅳ group was higher than that in the stage Ⅰ group,the group with lymph node metastasis was higher than that without lymph node metastasis,and the highly differentiated group was higher than the poorly differentiated group in clinical specimens(P＜0.05);COX regression analysis showed that Federation International of Gynecology and Obstetrics(FIGO)staging and PDIA3 expression level were the independent risk factors of prognosis in EC patients;the survival time of EC patients with high expression of PDIA3 in the TCGA database was shorter than that of the low expression group(P＜0.05),and the survival rate of clinical patients with positive PDIA3 was shorter than that of the neg-ative group(P＜0.05);Transwell assay,scratch assay,and CCK-8 assay showed that the invasion and migration ability of cells in the PDIA3 inhibition group was significantly reduced compared to the normal group and negative control group(P＜0.05).Conclusion PDIA3 is highly expressed in EC tissues and is associated with the malignancy of tumors and poor prognosis of patients;targeted inhibition of PDIA3 expression can reduce the proliferation,invasion,and migration ability of Ishikawa cells.

Objective To compare ferroptosis characteristics and motor dysfunction across different mouse models of Parkinson's disease(PD).Methods Animal models of PD were divided into five groups:the control group,sham-operated group,6-hydroxydopamine(6-OHDA)group,1-methyl-4-phenyl-1,2,3,6-tetrahydropyri-dine(MPTP)group,and lipopolysaccharide(LPS)group.Differences in ferroptosis-related features and motor dysfunction across these groups were evaluated through behavioral observation,histopathological examination,protein analysis,and assessment of lipid peroxidation and oxidative stress levels.Results In all three model groups,the number of TH-positive neurons in the substantia nigra on the lesioned side was significantly reduced,accompanied by evident neuronal degeneration and a marked decrease in Nissl bodies.Behavioral assessments revealed that the 6-OHDA group displayed the most severe motor deficits.In the substantia nigra,FTH1 protein expression was significantly downregulated in the 6-OHDA,LPS,and MPTP groups(P<0.01,P<0.05,P<0.01),respectively,while GPX4 expression was also reduced(P<0.001,P<0.001,P<0.01).Malondialdehyde(MDA)levels were significantly elevated in both the 6-OHDA and MPTP groups(P<0.05,P<0.05).Glutathione(GSH)assays demonstrated markedly reduced levels in the 6-OHDA,MPTP,and LPS groups(P<0.01,P<0.001,P<0.001).Reactive oxygen species(ROS)detection revealed a significant increase in the MPTP and LPS groups(P<0.05,P<0.05).Conclusion The 6-OHDA model is well suited for studying PD-related behaviors and the underlying mechanisms of motor symptoms,the MPTP model is particularly effective for investigating the ferroptosis pathway,and the LPS model serves as a valuable complement for research on neuroinflammation mechanisms.