Objective To explore the short-term efficacy of endoscopic submucosal dissection (ESD) in the treatment of early carcinoma in the remnant stomach. Methods A retrospective study was conducted on 45 patients with early residual gastric cancer underwent ESD at the Endoscopy Center of Zhongshan Hospital, Fudan University from December 2014 to April 2024, with a total of 45 lesions. The patients were divided into an anastomotic group (n=15) and a non-anastomotic group (n=30) based on the location of tumor occurrence, and their clinical data, endoscopic diagnosis and treatment, and histopathological conditions were compared between the two groups. Results All 45 patients had lesions with redness and erosion. There were 9 cases of poor lifting of submucosal injection in the anastomotic group and 2 cases in the non-anastomotic group, respectively, and the difference was statistically significant (P<0.05). ESD surgery was performed on 13 lesions in the anastomotic group and 28 lesions in the non-anastomotic group, with surgery times of 80.00 (50.00, 100.00) min and 55.00 (43.75, 80.00) min, respectively. The difference in surgery time between the two groups was statistically significant (P=0.03). Among the 45 patients, ESD surgery achieved curative resection in 35 cases, including 11 cases in the anastomotic group and 24 cases in the non-anastomotic group, with no statistically significant difference. Conclusions Careful preoperative evaluation of early carcinoma in the remnant stomach is essential to prevent oversight. Lesions at anastomotic sites and suture lines present higher technical challenges for complete resection. ESD is safe and effective, with auxiliary traction technique available when necessary.

Objective To compare the effect of immediately wearing different running shoes and foot-strike patterns on the in vivo Achilles tendon(AT)length and loading of habitual rearfoot striking(RFS)runners.Methods Eleven male running enthusiasts were recruited(age:33.7±8.7 years old,running experience:5.3±2.3 years).They were randomly assigned to wear cushioned running shoes or minimalist shoes,and run at 10 km/h on a Bertec 3-D force platform with forefoot striking(FFS)and RFS,respectively.The ultrasound imaging probe was fixed at the junction of the AT and the me-dial head of the gastrocnemius muscle with an elastic bandage,synchronized with the Vicon high-speed infrared motion capture system,which collected and calculated real-time length changes and loading characteristics of the AT in vivo.A 2×2 two-way ANOVA was used to determine the effect of different running shoes(cushioned running shoes vs.minimalist shoes)and foot strike patterns(FFS vs.RFS)on the in vivo AT loading characteristics during running,and the significance level α was set at 0.05.Results There was a significant interaction between footwear type and footstrike patterns on the foot strike angle.Within the same shoe condition(cushioned or minimalist),foot strike angle(FSA)was significantly greater during RFS than during FFS(P<0.05).Moreover,during RFS,FSA was significantly greater in cushioned shoes than in minimalist shoes(P<0.05).In addition,under acute manipulations of footwear and strike pattern,the instantaneous AT loading rate also exhibited a signifi-cant footwear and foot strike patterns interaction(P<0.05).The instantaneous AT loading rate was sig-nificantly greater when immediately wearing minimalist shoes with RFS than when wearing cushioned running shoes with RFS,as well as significantly greater when immediately wearing cushioned running shoes with FFS than when running with RFS(P<0.05).In addition,there was no interaction effect for the other parameters,except the significant group or time main effects(P<0.05).The peak AT force,average AT loading rate,AT impulse,peak AT stress,peak AT length change,and AT work during FFS were significantly greater than those during RFS(P<0.05).What's more,the peak AT force when immediately wearing minimalist shoes was significantly greater than that when wearing cushioned run-ning shoes(P<0.05).Conclusion It is suggested that immediately wearing minimalist shoes and/or transi-tioning to FFS could provide higher tendon loading intensity,which might improve the morphology and mechanical properties of the AT.However,the increased AT loading rate also indicated that if RFS was maintained while running in minimalist shoes,the reduced cushioning could have led to a greater risk of injury without an appropriate adaptation process.

Objective To compare the effect of immediately wearing different running shoes and foot-strike patterns on the in vivo Achilles tendon(AT)length and loading of habitual rearfoot striking(RFS)runners.Methods Eleven male running enthusiasts were recruited(age:33.7±8.7 years old,running experience:5.3±2.3 years).They were randomly assigned to wear cushioned running shoes or minimalist shoes,and run at 10 km/h on a Bertec 3-D force platform with forefoot striking(FFS)and RFS,respectively.The ultrasound imaging probe was fixed at the junction of the AT and the me-dial head of the gastrocnemius muscle with an elastic bandage,synchronized with the Vicon high-speed infrared motion capture system,which collected and calculated real-time length changes and loading characteristics of the AT in vivo.A 2×2 two-way ANOVA was used to determine the effect of different running shoes(cushioned running shoes vs.minimalist shoes)and foot strike patterns(FFS vs.RFS)on the in vivo AT loading characteristics during running,and the significance level α was set at 0.05.Results There was a significant interaction between footwear type and footstrike patterns on the foot strike angle.Within the same shoe condition(cushioned or minimalist),foot strike angle(FSA)was significantly greater during RFS than during FFS(P<0.05).Moreover,during RFS,FSA was significantly greater in cushioned shoes than in minimalist shoes(P<0.05).In addition,under acute manipulations of footwear and strike pattern,the instantaneous AT loading rate also exhibited a signifi-cant footwear and foot strike patterns interaction(P<0.05).The instantaneous AT loading rate was sig-nificantly greater when immediately wearing minimalist shoes with RFS than when wearing cushioned running shoes with RFS,as well as significantly greater when immediately wearing cushioned running shoes with FFS than when running with RFS(P<0.05).In addition,there was no interaction effect for the other parameters,except the significant group or time main effects(P<0.05).The peak AT force,average AT loading rate,AT impulse,peak AT stress,peak AT length change,and AT work during FFS were significantly greater than those during RFS(P<0.05).What's more,the peak AT force when immediately wearing minimalist shoes was significantly greater than that when wearing cushioned run-ning shoes(P<0.05).Conclusion It is suggested that immediately wearing minimalist shoes and/or transi-tioning to FFS could provide higher tendon loading intensity,which might improve the morphology and mechanical properties of the AT.However,the increased AT loading rate also indicated that if RFS was maintained while running in minimalist shoes,the reduced cushioning could have led to a greater risk of injury without an appropriate adaptation process.

Objective:To understand the molecular characteristics of the mutant strain of polymerase basic protein 2 (PB2) gene of influenza A (H1N1pdm) in Guangdong province, and to explore its specific molecular sites, so as to provide scientific basis for the prevention and control of influenza virus.Methods:Throat swab samples were collected from 2 cases infected with PB2 gene variant strains for virus isolation, and 23 influenza virus strains were selected from Guangdong province for sequencing analysis. The reference sequences and vaccine strain sequences provided by GISAID were used to perform evolutionary analysis on hemagglutinin (HA) and PB2 genes. Virus strain antigen analysis and neuraminidase (NA) inhibition test were carried out. PB2 protein model was constructed and polymerase activity was analyzed.Results:H399N amino acid mutation occurred in the HA gene of PB2-D701N and PB2-A271S variant strains, both of which belonged to the branch of 6B.1A.5a.2a. They belonged to the same big branch and different small branches as the vaccine strain A/Victoria/4897/2022, and they are all vaccine-like strains. In the three-dimensional structure, the mutations of PB2-D701N and PB2-A271S change charge and hydrophobicity.Conclusions:PB2-D701 and A271 were highly conserved, and PB2 mutant strains were not the dominant strains. The PB2 mutant had high antigenicity with the vaccine. The PB2 mutant strain is sensitive to NA inhibitors. The three-dimensional model predicted that PB2-D701N mutation could enhance virulence and affect transmissibility of influenza virus, while PB2-A271S mutation could affect polymerase activity and polymerase complex synthesis of influenza virus.

Abstract: Objective　To analyze the serotypes, drug resistance, and molecular epidemiological characteristics of non-typhoidal Salmonella in Chaozhou City, Guangdong Province in 2022, providing pathogenic support for the effective prevention and control of foodborne diseases. Methods In 2022, human-derived non-typhoidal Salmonella strains and food-derived strains were isolated through sentinel surveillance and food risk monitoring in Chaozhou City, Guangdong Province. The strains were subjected to serotyping, drug susceptibility testing, and whole-genome sequencing analysis. Results A total of 1 257 fecal specimens were collected for diarrhea surveillance in Chaozhou City in 2022, and 58 strains of non-typhoidal Salmonella were detected, with an isolation rate of 4.61%. Among 116 foodstuffs, 2 strains of non-typhoidal Salmonella were detected, with an isolation rate of 1.72%. Early childhood was the main age group for infection (14/144, 9.72%). The highest detection rate was in June (12/123, 9.76%). The 58 human-derived non-typhoidal Salmonella strains were classified into 12 serotypes, with Salmonella typhimurium being the most prevalent (44/58, 75.86%). In terms of drug resistance, 55 strains showed resistance, with a resistance rate of 91.67%, among which ampicillin, tetracycline, chloramphenicol, and streptomycin had the highest resistance rates, 76.67%, 70.00%, 67.67%, and 60.00%, respectively. Forty-nine strains were multi-resistant, accounting for 81.67%. From the molecular characteristics standpoint, multilocus sequence typing (MLST) divided the strains into 14 sequence typing (ST), with Salmonella typhimurium ST34 being the dominant type. Whole-genome single nucleotide polymorphism (wg-SNP) evolutionary analysis revealed high genetic heterogeneity among the studied strains, with Salmonella typhimurium clustering together and showing close evolutionary relationships to clinical samples of Salmonella enteritidis. Conclusions In 2022, the non-typhoidal Salmonella strains from Chaozhou showed a diversity of serotypes, with Salmonella typhimurium being predominant and exhibiting severe multidrug resistance. Molecular typing revealed diverse evolutionary characteristics.

Objective To investigate the correlation between serum miR-27b-3p and N-terminal propeptide of type Ⅰ procollagen (PINP) and C-terminal telopeptide of type Ⅰ collagen 1 (CTX-1) in postmenopausal women. Methods Forty-nine patients with postmenopausal osteoporosis treated in the Second Affiliated Hospital,Zhejiang Chinese Medical University from February 2022 to February 2023 were selected as study objects. They were divided into osteoporosis (OP) group (T≤-2.5SD,26 cases) and osteopenia (OPn) group (-2.5SD

Objective To investigate the correlation between serum miR-27b-3p and N-terminal propeptide of type Ⅰ procollagen (PINP) and C-terminal telopeptide of type Ⅰ collagen 1 (CTX-1) in postmenopausal women. Methods Forty-nine patients with postmenopausal osteoporosis treated in the Second Affiliated Hospital,Zhejiang Chinese Medical University from February 2022 to February 2023 were selected as study objects. They were divided into osteoporosis (OP) group (T≤-2.5SD,26 cases) and osteopenia (OPn) group (-2.5SD

Objective To investigate the etiological characteristics of food poisoning isolates of Vibrio parahaemolyticus (VP) from 2019 to 2021 in Zhongshan City. Methods A total of 37 strains of Vibrio parahaemolyticus isolated from 8 food poisoning incidents in Zhongshan City from 2019 to 2021 were collected, including 1 residual food isolate and 36 human isolates. The genetic correlation of Vibrio parahaemolyticus food poisoning isolates in this region was analyzed by serological typing, virulence gene detection (TLH, TDH, and TRH), drug sensitivity test, pulsed field gel electrophoresis (PFGE) and multipoint sequence typing (MLST). Results The 37 strains of Vibrio parahaemolyticus were divided into 4 serotypes: O3:K6, O10:K4, O4:K8, and O4:KUT. The tdh+ and trh- were the main virulence genotypes, accounting for 97.30% (36/37). The drug resistance rate of cefazolin was 40.54% (15 strains R, 22 strains I), and no multidrug-resistant strains were found. The 37 VP strains were divided into 23 PFGE types and 6 cluster groups, with correlation coefficients ranging from 60.4%-100%. The multipoint sequencing typing showed that the 37 VP strains were divided into 9 ST types and 3 complex groups, of which ST3 type was the main type (23 strains, 62.1%). Conclusion This study has found that the dominant virulence types of Vibrio parahaemolyticus food poisoning isolates in Zhongshan City from 2019 to 2021 are tdh⁺ and trh^-, and 37 representative strains can be divided into 6 PFGE clusters and 9 ST types with MLST type being mainly ST3. This study has identified the rare serotype O10:K4 which has caused an increase in the proportion of food poisoning events, suggesting that we should strengthen detection and be alert to the risk of continued local epidemics of new rare serotype strains.

OBJECTIVE: To investigate the effects of overexpression of long noncoding RNA (lncRNA) MEG3 on the proliferation and invasion of glioblastoma U251 cells by suppressing the expression of hypoxia inducible factor 1 METHODS: The expression of lncRNA MEG3 and HIF1 RESULTS: The expression of MEG3 was significantly lower and HIF1 CONCLUSIONS MEG3 overexpression inhibits the proliferation and invasion of U251 cells through suppressing the expression of HIF1
Apoptosis ; Cell Line, Tumor ; Cell Movement/genetics* ; Cell Proliferation/genetics* ; Gene Expression Regulation, Neoplastic ; Glioblastoma/genetics* ; Humans ; MicroRNAs ; RNA, Long Noncoding/genetics*