Background: Skin wounds can occur for many reasons, including UV radiation, mechanical burns, trauma, and chronic inflammation caused by metabolic factors. In Mongolia, as of 2022, household burns were the top 5 causes of injuries, the leading cause of injuries in children aged 0-5, and accounted for 18.4% of all illnesses in children aged 0-5. Skin burns and wounds take a long time to heal, which causes emotional, aesthetic, and economic stress for doctors. Developing in novative, effective, and low-side effects therapeutic products to improve wound healing is of great clinical and economic importance. The lack of research on the effects of bacterial metabolites and aloe vera (Aloe barbadensis Miller) on burn wounds was the basis for our research. Aim: To determine the effects of a mixture of bacterial metabolites and aloe vera on burn wounds in experimental animals. Materials and Methods: Eight-week-old BALB/c mice were divided into three groups: control group, bacterial metabo lite group, and aloe vera group. The mice were anesthetized, and the dorsal hair was shaved over a 2×2 cm area using an electric clipper. A second-degree burn wound model was created by exposing the shaved dorsal skin to hot air at 200°C for 10 seconds. A total of 100 μL of a bacterial metabolite suspension mixed with aloe vera at a 1:1 ratio was applied evenly to the center of the wound. Wound images were captured daily, and wound areas were measured using ImageJ software. Healing was evaluated on days 7, 14, and 21 of treatment and compared with the control group. The initial wound size was defined as 100%, and wound closure percentages were calculated accordingly. Burn skin tissues from both experimen tal and control groups were subjected to immunofluorescence staining for the vascular marker α-SMA (Thermo Fisher Scientific). Statistical analysis: The differences between the control and experimental groups were analyzed using an independent t-test using GraphPad Prism 10 software, and the mean, standard deviation, and statistical correlation were calculated. Results: The effect of the mixture of bacterial metabolites and aloe vera (Aloe barbadensis Miller) on the healing of burn wounds in experimental mice was compared with the control group. The wound size at 7 days was 82.1% in the control group and 83.7% in the experimental group (p<0.197), at 14 days was 62.6% in the control group and 58.1% in the exper imental group (p<0.0001), at 21 days was 50.7% in the control group and 32.7% in the experimental group (p<0.0001). No statistically significant difference in wound healing was observed between the groups at day 7; however, significant differences were observed at days 14 and 21. Immunofluorescence analysis revealed uniform expression of α-SMA-pos itive cells in the experimental group at 7, 14, and 21 days of treatment, whereas α-SMA expression was not detected in the control group. Conclusion The combination of bacterial metabolites and aloe vera promotes wound healing in experimental mouse burn wounds.

Background: Plants rich in essential oils belong to more than 50 families, including Asteraceae, Rutaceae, and Lamiaceae. These plants are widely used in food, traditional and modern medicine, perfumery, cosmetics, and pharmaceutical industries. Approximately 3,000 types of essential oils have been identified, of which more than 300 are utilized for industrial and commercial purposes. Essential oils exhibit antimicrobial, anti-inflammatory, anticancer, cytotoxic, and antioxidant activities and are therefore widely applied in medical research. In Mongolia, the extreme climate, distinct ecological regions, and the prevalence of pastoral livestock husbandry provide a scientific basis for investigating essential oil–containing plants, their chemical composition, and biological activities. Among them, Juniperus Sabina L. is of particular theoretical and practical interest. Aim: To determine the non-toxic dose of essential oil extracted from Juniper Sabina L. on neural cells. Materials and Methods: This study was conducted at the Institute of Bio-Medicine, MNUMS. Ethical approval was obtained from the Research Ethics Committee of MNUMS (Meeting date: November 21, 2025; Protocol No. 2025-26/0301). BV2 microglial and N2a neuronal cell lines were cultured under standard conditions at 37°C in a 5% CO₂ incubator. Cells were maintained in appropriate culture media, and their growth and morphology were monitored microscopically before experimentation. Cell viability was assessed using the MTT assay. BV2 microglial cells were seeded into 96-well plates at a density of 2×10⁴ cells/well and incubated for 24 hours. Essential oil was added at concentrations ranging from 50–500 µg/mL and incubated for an additional 24 hours. After treatment, MTT reagent was added and incubated for 3 hours. The resulting formazan crystals were dissolved in dimethyl sulfoxide (DMSO), and absorbance was measured at 630 nm. Cell viability was calculated based on absorbance values to determine the non-toxic concentration of the essential oil. Result: The cytotoxic effects of the essential oil were evaluated in BV2 microglial and N2a neuronal cells using the MTT assay. In N2a neuronal cells, concentrations of 50, 100, 200, and 300 µg/mL did not significantly affect cell viability, whereas 400 and 500 µg/mL reduced cell viability, indicating cytotoxic effects. Conclusion According to MTT assay results, the essential oil of Juniperus sabina showed no cytotoxic effect on N2a neuronal cells at concentrations of 50, 100, 200, and 300 µg/mL, while cytotoxic effects were observed at 400 and 500 µg/mL. In BV2 microglial cells, concentrations of 50, 100, and 200 µg/mL were non-toxic, whereas concentrations above 300 µg/mL exhibited cytotoxic effects.

Background: Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by difficulties in social interaction, restricted interests, and repetitive behaviors. Evaluating short-term treatment progress and outcomes in children with ASD is important for determining subsequent treatment goals. Aim: This study aimed to evaluate the outcomes during the first 3 months of applied behavior analysis (ABA)-based intervention using changes in Autism Treatment Evaluation Checklist (ATEC) scores. Materials and Methods: A prospective observational study was conducted among 21 children with ASD. Caregivers completed the 77-item ATEC before initiation of treatment and again after 3 months. Scores were calculated for four ATEC domains: Speech/Language/Communication, Sociability, Sensory/Cognitive Awareness, and Health/Physical/Behavior. Result: The mean age of participants was 5.51±1.45 years, and 16 children (76.19%) were male. Mean baseline Clinical Global Impressions–Severity (CGI-S) score was 4.71±0.72, and participants received a mean of 10.00±1.38 hours of ABA-based intervention per week. Mean total ATEC score decreased from 72.71±7.60 at baseline to 67.14±7.81 after 3 months, corresponding to a mean change of -5.57±2.18 points (-7.66%; t=11.706, p<0.001, d=2.55). Speech/Language/ Communication decreased by 7.05%, Sociability by 6.96%, and Sensory/Cognitive Awareness by 6.56%. The largest relative change was observed in the Health/Physical/Behavior domain (-1.90±1.14; -9.80%; p<0.001). Wilcoxon signed-rank tests were significant across all ATEC domains (p<0.001). Sixteen children (76.19%) met the study-defined criterion of more than a 5-point reduction. Baseline CGI-S was correlated with pre-post ATEC change using Pearson correlation (r=0.466, p=0.033), whereas the corresponding Spearman analysis was not statistically significant (ρ=0.349, p=0.121); therefore, this association could not be considered a robust predictor. Weekly ABA treatment hours were not significantly associated with ATEC change (r=-0.183, p=0.427), and age was also not associated with change (r=-0.043, p=0.855). Conclusion Changes across all ATEC domains were observed during the period in which children received ABA-based intervention, suggesting that some treatment-related changes may be detectable over a relatively short period in everyday settings. However, because only two assessment points were available, the rate and sequence of change could not be determined. Future studies should include larger samples, a comparison group, repeated assessments over 6–12 months, and independent developmental and clinical outcome measures.

Background: Dental caries remains a major public health concern among children and adolescents in Mongolia. The mineral composition of drinking water-particularly fluoride and other essential minerals plays a crucial role in enamel mineralization and caries prevention. Fluoride directly influences the demineralization–remineralization balance of dental enamel and forms a temporary layer of fluid fluorapatite on the enamel surface when exposed to acids, thereby reducing the critical pH for dissolution and increasing acid resistance. In addition, fluoride inhibits the acid-producing metabolism of bacteria such as s.mutans within the biofilm and lowers biofilm acidity, thus slowing the rate of demineralization. Therefore, determining the relationship between population groups and water mineralization is essential for developing effective preventive strategies. Aim: To determine the severity and prevalence of dental caries among 5-, 12-, and 15-year-old children living in the 21 provinces of Mongolia and to evaluate the effects of selected minerals in drinking water (Ca, Mg, Cl, F) on dental caries. Materials and Methods: The study utilized oral examination data from a total of 421,970 children aged 0-17 years across 21 provinces and 321 soums in Mongolia. Sampling was conducted in Ulaanbaatar city (48.1%) and in all 21 rural provinces. Dental caries was assessed according to WHO criteria, and the effects of minerals in drinking water were analyzed using linear regression. Result: The average caries severity of primary teeth in 5-year-old children was 3.0-3.1 dft, which was higher than the caries severity of permanent teeth in 12- and 15-year-olds (1.54DMFdft–1.93 DMFT). Among 5-year-olds, boys had a slightly higher caries severity (3.17±3.52) compared to girls (2.92±3.40), while in the 12- and 15-yearold groups, girls showed higher caries severity than boys. Overall, 5566% of children had at least one carious tooth, and the prevalence among 5-year-olds was 63.5-66.2%. Linear regression analysis showed that fluoride (beta=–0.449, p<0.001) and magnesium (beta=–0.031, p<0.001) in drinking water had a statistically significant protective effect in reducing dental caries in both primary and permanent teeth. Conclusion The findings indicate that fluoride (F) and magnesium (Mg) in drinking water are important protective elements against dental caries, whereas calcium (Ca) and chloride (Cl) show no consistent association with caries experience.

Background: Allergic and atrophic forms of chronic rhinitis are common among the population, characterized by a chronic progressive course with a tendency toward increasing prevalence. Worldwide, allergic rhinitis affects approximately 10–40% of adults and 2–25% of children. According to a study conducted in Mongolia, the prevalence of allergic rhinitis among adults in Ulaanbaatar was reported to be 23.6%, with an increasing trend anticipated in the future. In allergic and atrophic rhinitis, structural alterations occur in the nasal mucosa, including disruption of the normal architecture of the pseudostratified epithelium and ciliated cells, accompanied by changes in goblet cell number and dysfunction of their activity, resulting in a complex pathological process. In otorhinolaryngology practice in Mongolia, there is a relative lack of studies investigating the ultrastructural changes of the nasal mucosa in allergic and atrophic rhinitis. Aim: To compare ultrastructural and histological changes of the nasal mucosa in allergic and atrophic rhinitis. Materials and Methods: Ethical approval was obtained from the Research Ethics Review Committee of the Mongolian National University of Medical Sciences at the meeting held on April 24, 2020 (Protocol No. 2020/З-04). Appropriate ethical permission was also obtained from the EMJJ ENT Hospital. Nasal mucosal tissue samples were collected from surgically resected specimens of patients aged 20–45 years diagnosed with allergic and atrophic rhinitis. The mucosal specimens were processed at the Institute of Biomedical Sciences of the Mongolian National University of Medical Sciences. Samples were fixed and dehydrated according to the protocol, coated with 100 Å gold, and examined using a scanning electron microscope (FEI Quanta FEG 250, USA). In addition, paraffin blocks were prepared, sectioned at 4 μm thickness, stained with Hematoxylin and Eosin, and analyzed histologically. Results: In allergic rhinitis, the pseudostratified epithelium was relatively preserved; however, disorganization of ciliated cells, partial loss of cilia, and goblet cell hyperplasia were observed. In contrast, atrophic rhinitis showed epithelial thinning, near-complete loss of ciliated structures, glandular atrophy, and submucosal fibrosis. Conclusion Allergic rhinitis is associated with relatively mild and predominantly functional mucosal alterations, whereas atrophic rhinitis demonstrates severe structural damage with irreversible impairment of mucosal protective function.

Background: Background: In recent years, ensuring a safe and healthy working environment for mining employees, improving working conditions, protecting and promoting health, and paying attention to other factors affecting health have become essential requirements. While infectious diseases, noncommunicable (chronic) diseases, nutritional disorders, occupational injuries, and mental health conditions are the main indicators for assessing the health impact on mining workers, there is still a lack of research on noncommunicable diseases among mining sector employees in Mongolia. This gap served as the basis for the present study. Aim: To study the risk factors of noncommunicable diseases among employees of Erdenet Mining Corporation Materials and Methods: A total of 303 employees from 15 divisions of Erdenet Mining Corporation participated in the study. Participants belonging to high-risk groups for noncommunicable diseases were selected using a purposive sampling method. The survey questionnaire was developed based on the WHO STEP wise approach to Surveillance (STEPS) of noncommunicable disease risk factors. Results: Of all participants, 70% were male and 30% were female, with an average age of 46 years. The average total duration of employment was 23 years, of which male employees had worked an average of 19.3 years and female employees 17.3 years at Erdenet Mining Corporation. Among participants, 48.5% had never smoked, while 38.3% were current smokers. When analyzing alcohol consumption by job position, it was found that 85.7% of managers and 66.7% of administrative and decoration workers had high levels of alcohol consumption. Regarding physical activity, 23% of male and 15.2% of female employees reported engaging in active exercise more than three days per week, indicating that male employees were more physically active than females. In terms of dietary habits, 28.3% of males and 17.6% of females did not control their salt intake. As for eating patterns, 53.6% of employees regularly had breakfast, 77.3% regularly had lunch, and 76.6% regularly had dinner at a fixed time. Conclusions 1. Among the employees surveyed, 48.5% had never smoked, while 38.3% were current smokers. Daily smoking was most prevalent among employees aged 35–54 years (74.2%), with the 25–34 age group also showing a relatively high rate of smoking. Regarding alcohol consumption, 38.2% of male employees reported drinking vodka or similar alcoholic beverages. Alcohol consumption was significantly higher among males compared to females, indicating that it is a major risk factor for noncommunicable diseases (p=0.003). 2. In terms of dietary patterns, 53.6% of employees reported regularly having breakfast, 77.3% regularly had lunch, and 76.6% regularly had dinner. Eating habits varied by gender. Concerning physical activity, 23% of male employees and 15.2% of female employees engaged in regular exercise, indicating that male employees were more physically active than females. The study found that lifestyle and behavioral factors such as smoking, alcohol consumption, insufficient physical activity, and poor dietary habits contribute to an increased risk of noncommunicable diseases among employees.

Background: Researchers have identified that occupational exposures related to mining operations-such as dust, noise, and vibration—can increase the risk of developing occupational diseases, including pneumoconiosis, hearing loss, and spinal injuries, as well as noncommunicable conditions such as hypertension and cardiovascular diseases. Work-related stress, living away from family, reliance on portable food services, and shift work systems also contribute to weight gain and negatively affect endocrine function. Aim: To investigate the prevalence of metabolic syndrome and noncommunicable diseases among employees of Erdenet Mining Corporation Materials and methods: A total of 303 employees from 15 divisions of Erdenet Mining Corporation participated in the study. Participants included employees with 10–20 years or more of service; 15% were female, and 75% were technical workers. Employees belonging to high-risk groups for noncommunicable diseases were selected using a purposive sampling method. Results: When analyzing body mass index (BMI) by occupation, overweight or obesity was found among 79.2% of service workers, 65% of electricians, 100% of fuel distributors, 77.8% of decorators, 75.5% of mechanics, 94.7% of specialists, 86.4% of professional workers, 75.7% of operators and engineers, and 88.9% of administrative staff. Central obesity was also analyzed by job position, revealing that 79.2% of service workers, 60% of laboratory staff, 35% of electricians, 64.9% of operators and engineers, and 66.7% of administrative employees had central obesity. Among all participants, 20.7% had a previous history of cardiovascular disease, of whom 71.4% were male and 28.6% were female. The mean blood glucose level among participants was 6.0 mmol/L, with a median of 5.6 mmol/L, a minimum of 3.9 mmol/L, and a maximum of 18.3 mmol/L. The median glucose level was 0.2 mmol/L lower than the national average. No statistically significant difference in blood glucose levels was found between males and females (p = 0.135). By job position, elevated blood glucose levels (prediabetic range) were found in 62.5% of service workers, 40% of laboratory staff, 35% of electricians, 71.4% of managers, and 55.6% of administrative employees. Conclusion The findings indicate an increasing trend in metabolic and cardiovascular risk factors among employees of Erdenet Mining Corporation SOE.

Background: In the treatment of plaque-induced gingivitis, in addition to mechanical plaque control, the use of chemical plaque control such as mouth rinses for a certain period has shown a positive effect on treatment outcomes and prognosis. Aim: To evaluate the efficacy of a probiotic mouthwash in the treatment of plaque-induced gingivitis. Materials and Methods: A randomized controlled clinical trial was designed for a period of 2 weeks on 45 systemically healthy subjects between 20 and 25 years having plaque-induced gingivitis. The study population was divided into three groups. Group 1-15 subjects were advised experimental (probiotic) mouthwash. Group 2-15 subjects were advised positive control (chlorhexidine) mouthwash and Group 3-15 subjects into a negative control group. Oral prophylaxis was done for all groups at baseline. After the proper oral hygiene instructions, groups 1 and 2 were instructed to rinse their mouth with 15 ml of their respective mouthwashes, for 1 min twice daily, 30 min after brushing. Clinical parameters such as Full mouth plaque score (FMPS), Full mouth bleeding score (FMBS) were assessed at baseline, 2 weeks respectively. Results: At baseline, there was no statistically significant difference between the groups in terms of FMBS and FMPS mean values (p=0.174, p=0.887). At day 14, the FMPS, FMBS mean values were significantly reduced by all treatment modalities ranking probiotic and chlorhexidine is greater than negative control group (p<0.001, p<0.001). Conclusion The probiotic mouthwash was effectively used as an adjunct to mechanical plaque control in the treatment of plaque-induced gingivitis.

Background: According to WHO research, there are approximately 24 million people living with schizophrenia worldwide and schizophrenia is characterized by a combination of psychotic and non-psychotic symptoms. Since the cause of the disease is not fully understood, antipsychotic medications are used as symptomatic treatment. According to the 2022 statistics of the NCMH, 718 people with schizophrenia are being treated under active surveillance in Mongolia. The reason for conducting this study is that the manifestation of drug side effects resulting movement disorders in patients with schizophrenia, which has not been studied in Mongolia. Aim: To investigate the relationship between adherence of medication regimen and abnormal involuntary movements in patients with schizophrenia. Materials and Method: The study was conducted using a descriptive method, cross-sectional design, purposive sampling with the questionnaire and standardized tests. Ethical approval for this study was approved by the NCMH (№3/77 30th of January, 2023) and Research Ethics Review Committee of MNUMS (№2023/3-02). Each participant was asked to complete 5 groups of 36 questionnaires, and standard tests were used to assess patients’ adherence to medication regimens (Morisky scale) and abnormal involuntary movement scale (AIMS). The study was conducted between March and August 2023, and the results were summarized and analyzed using STATA 14 software. Results: The study included 209 patients with schizophrenia, aged 18-79 years, of whom 47.4% (n=99) were male and 52.6% (n=110) were female (p=0.21). Of the participants, 28.2% (n=59) had less than secondary education, 76.5% (n=160) were unmarried, and 85.2% (n=178) had a disability due to mental health. 32.5% (n=68) of the patients with schizophrenia in the study used a combination of typical and atypical medications, and the most commonly used antipsychotic drugs were haloperidol (30.6%), chlorpromazine (26.8%), levomepromazine (25.8%), risperidone (24.4%), and quetiapine (21.1%). 1.4% (n=3) of the patients had good, 52.6% (n=110) had moderate, and 45.9% (n=96) had poor adherence to the medication regimen (Cronbach’s α=0.781). However, according to the results of the test for assessing abnormal involuntary that are performed without self-control, 49.76% (n=104) responded that they felt more sensitive to facial and oral movements, and 44.5% (n=93) to limb movements. The patients’ adherence to the medication regimen was statistically significant with facial and oral movements (n=104; p=0.036) and general body movement disorders (n=94; p=0.05). Conclusion 32.5% of patients with schizophrenia were taking typical and atypical antipsychotics, and 45.9% had poor adherence to medication regimens and were more likely to exhibit clinical forms of abnormal involuntary movements, including facial (p=0.036) and general movement disorders (p=0.05).

Introduction: Toll like receptors (TLRs) are a class of proteins that key role in the innate immune system. The SOCS1 and SHP2 proteins are negative-feed loop inhibitors of signaling of JAK/STAT and TLRs pathways. Purpose: To determine negative regulator protein activation which is activated through TLR7 ligand/IFN-γ signal transduction in endothelial cells. Methods: We used mouse aortic linear endothelial cell (END-D); protein expressio was detected by western blotting Results: We analyzed a time dependent stimulation effects of negative regulator proteins stimulated by TLR7 ligand/IFN-γ in endothelial cell cultures. Imiquimod of 10 μg/ml treatment of 1 hr was followed by 100 ng/ml IFN-γ stimulation for 1-8hr to analysis of negative regulator SOCS1 and SHP2 protein expression.
In untreated cells, there was low activations of negative regulator SOCS1 and SHP2 proteins. IFN-γ stimulation alone had increased SOCS1 and SHP2 protein expressions, also imiquimod treatment highly elevated SOCS1 and SHP2 expressions. However imiquimod and IFN-γ doubled treatment have decreased activation of negative regulator SOCS1 and SHP2 proteins. These findings suggest SOCS1 and SHP2 proteins are inhibitors in the TLR7 ligand/IFN-γ signaling. Conclusion Negative regulators, SOCS1 and SHP2 strongly suppressed activations of TLR7 ligand/IFN-γ signaling