BACKGROUND:It is urgent to improve the study on the molecular mechanism of Ban's Culuan Zhuyun Decoction improving oocyte quality in polycystic ovary syndrome.OBJECTIVE:To observe the effects of Ban's Culuan Zhuyun Decoction on oocyte quality in a mouse model of polycystic ovary syndrome and to explore the underlying mechanisms of its intervention in polycystic ovary syndrome.METHODS:Subcutaneous injection of dehydroepiandrosterone sulfate was used to establish the polycystic ovary syndrome model in 21-day-old female Kunming mice,and the treatment was conducted for 21 consecutive days.The estrous cycle and pregnancy was recorded.ELISA was used to detect serum sex hormone levels.The rate of apoptosis in oocytes was detected using Annexin V staining.The level of reactive oxygen species in oocytes was detected using dichlorodihydrofluorescein diacetate.The condition of spindle bodies and chromosomes in oocytes were detected using the immunofluorescence method.Network pharmacology and molecular docking were used to verify the binding properties of Ban's Culuan Zhuyun Decoction core active components and oocyte maturation-related factors(growth differentiation factor 9 and bone morphogenetic protein 15).Real-time fluorescence quantitative PCR and western blot were used to detect the mRNA and protein expression levels of growth differentiation factor 9 and bone morphogenetic protein 15 in oocytes,respectively.RESULTS AND CONCLUSION:(1)Ban's Culuan Zhuyun Decoction core active components(quercetin,kaempferol,and β-sitosterol)showed good binding activities with growth differentiation factor 9 and bone morphogenetic protein 15.(2)Ban's Culuan Zhuyun Decoction ameliorated the estrous cycle,regulated serum hormone,increased the pregnancy,decreased the rate of apoptosis,declined the level of reactive oxygen species,diminished the rate of abnormal spindle assembly and chromosome loss(P<0.01,P<0.05);and promoted the mRNA and protein expression of growth differentiation factor 9 and bone morphogenetic protein 15(P<0.05).Therefore,Ban's Culuan Zhuyun Decoction may improve the oocyte quality and increase the fertility of polycystic ovary syndrome mice by regulating the gene expression of growth differentiation factor 9 and bone morphogenetic protein 15.

BACKGROUND:It is urgent to improve the study on the molecular mechanism of Ban's Culuan Zhuyun Decoction improving oocyte quality in polycystic ovary syndrome.OBJECTIVE:To observe the effects of Ban's Culuan Zhuyun Decoction on oocyte quality in a mouse model of polycystic ovary syndrome and to explore the underlying mechanisms of its intervention in polycystic ovary syndrome.METHODS:Subcutaneous injection of dehydroepiandrosterone sulfate was used to establish the polycystic ovary syndrome model in 21-day-old female Kunming mice,and the treatment was conducted for 21 consecutive days.The estrous cycle and pregnancy was recorded.ELISA was used to detect serum sex hormone levels.The rate of apoptosis in oocytes was detected using Annexin V staining.The level of reactive oxygen species in oocytes was detected using dichlorodihydrofluorescein diacetate.The condition of spindle bodies and chromosomes in oocytes were detected using the immunofluorescence method.Network pharmacology and molecular docking were used to verify the binding properties of Ban's Culuan Zhuyun Decoction core active components and oocyte maturation-related factors(growth differentiation factor 9 and bone morphogenetic protein 15).Real-time fluorescence quantitative PCR and western blot were used to detect the mRNA and protein expression levels of growth differentiation factor 9 and bone morphogenetic protein 15 in oocytes,respectively.RESULTS AND CONCLUSION:(1)Ban's Culuan Zhuyun Decoction core active components(quercetin,kaempferol,and β-sitosterol)showed good binding activities with growth differentiation factor 9 and bone morphogenetic protein 15.(2)Ban's Culuan Zhuyun Decoction ameliorated the estrous cycle,regulated serum hormone,increased the pregnancy,decreased the rate of apoptosis,declined the level of reactive oxygen species,diminished the rate of abnormal spindle assembly and chromosome loss(P<0.01,P<0.05);and promoted the mRNA and protein expression of growth differentiation factor 9 and bone morphogenetic protein 15(P<0.05).Therefore,Ban's Culuan Zhuyun Decoction may improve the oocyte quality and increase the fertility of polycystic ovary syndrome mice by regulating the gene expression of growth differentiation factor 9 and bone morphogenetic protein 15.

Objective: To compare the dissolution behavior of notoginsenoside R1, ginsenoside Rb1 and Rg1 between the ordinary fine and ultrafine powder of Panax notoginseng and to investigate the effect of micronization on the dissolution of saponin ingredients in P. notoginseng. Methods: The oar method was adopted. The in vitro dissolution of the three kinds of saponins including notoginsenoside R1, ginsenoside Rg1 and Rb1 in different particle sizes was determined by HPLC. The dissolving rates of the powder in various particle sizes were compared. Results: The dissolving rates of the three kinds of saponins were greatly increased after ultramicro-pulverization. Conclusion: The ultramicro-pulverization is helpful to the dissolution of saponins in P. notoginseng pieces, and the particle size of the powder exerts the great influence on the dissolution of the three kinds of saponins.

PurposeTo observe the effects of rhIGF-1 on kidney in diabetic rats. MethodsUsing biochemistry, radioimmunoassay, molecular biology (RT-PCR). Results(1) 24 h UAER,24 h uriary volume in rhIGF-1 group is lower than that of diabetic control group; (2) The level of sertan IGF-1 ,kidney IGF-1 and IGF-1 mRNA in diabetic control group is lower than that of normal control group;The level of serum IGF-1 in rhIGF-1 group is higher than that of diabetic control group;no differences were found in the levels of kidney IGF-1 and kidney IGF-1 mRNA between diabetic control group and rhIGF-1 group; (3) The level of serum GH in diabetic control group is higher than that of normal control group; The level of serum GH in rhIGF-1 group is lower than that of diabetic control group; (4) rhIGF-1 might have some protective effects on diabetic nephropathy via electron microscope. ConclusionsrhIGF-1 doesn't increase the damage of diabetic kidney tissue.

As a novel interdisciplinary subject of information science and ethics, informational ethics first originated in the 1970s in western world, and has a short history of over 30 years. Besides computing ethics and network ethics, its research components still include all the ICT-induced ethical issues, such as ethical issues caused by tele-information, satellite technology, network technology, and ethical issues induced by mass media, news, libraries, and files, etc. As an example of the prosperity of informational ethics, library ethics emerged and develops rapidly recently. As a component of social libraries and scientific libraries, hospital libraries face a series of new issues, including hospital library ethics and the professional ethics of hospital librarians. This paper explores the ethical self-discipline of hospital librarians.

Objective To examine the relationship between the perioperative changes in cytokines and the postoperative complications after esophagecotomy. Methods Twenty-five ASA Ⅱ-Ⅲ patients undergoing esophagectomy were divided into two groups: group A without postoperative complication (n = 17), group B with postoperative complications including pulmonary infection (in 2 patients), pleural effusion (in 3 patients), cardiac arrhythmia(in 2 patients) and anastomosis hemorrage(in 1 patient) (n = 8). Blood samples were taken before anesthesia(T0 ), at skin incision(T1), 2h after chest was opened(T2 ), 60 min after lungs were inflated (T3) and 1,4, 24h after surgery (T4, T5, T6 ) for determination of serum IL-6, IL-8 and IL-10 concentrations. The durations of SIRS, the definition of which was set by American College of Chest Physicians /Society of Critical Care Medicine (ACCP/SCCM), was also recorded. Results The demographic data including age, sex and body weight were comparable between the two groups. There was also no significant difference in preoperative lung function, duration of surgery, blood loss during surgery and duration of unilateral lung ventilation between the two groups. The duration of SIRS was shorter in group A than that in group B. In both groups serum IL-6 and IL-8 levels increased significantly at T2 (after thoracotomy) reached their peak values at T4, and then gradually declined but were still significantly higher than the baseline values(T0). The serum IL-6 level was significantly higher at T6 (24h after surgery) in group B than that in group A. The serum IL-8 level was significantly higher at T3-6 in group B than that in group A. The IL-6/IL-10 and IL-8/IL-10 ratio were significantly lower at T5-6 in group A than those in group B. Conclusions The postoperative complications may occur due to the inflammatory response, and/or anti-inflammatory mediators insufficiency. The IL-6/IL-10 and IL-8/IL-10ratio may be of value in predicting the prognosis.

The changes of parathyroid hormone (PTH),calcitonin(CT)of three different anesthesia methods were observed perioperatively in 38 patients who were divided into two age groups (22 ~ 50 and 51 ~ 60). Postoperative serum Ca, P levels decreased as compared with preoperative values,espe.cially in the old. age groups with no statistic significance. These results indicate that during anesthesia and operative stress the CDS is inhibited and the regulation of the autonomic nervous system is affected, with obscure the relationship between parathyroid hormone, calcitonin and Ca, P with the alteration of Ca,P metabolism. Serum Ca may increase to the baseline by supplement of calcium gluconate during and after operation, but the operation after 4 ~ 5 days, serum Ca may also return to normal without any intervention.