Objective:To compare the diagnostic efficacy and consistency of fecal calprotectin (FC) detected by enzyme-linked immunosorbent assay (ELISA) and fluorescence immunochromatography assay (FICA) in assessing the disease activity of inflammatory bowel disease (IBD) .Methods:The paired-design diagnostic test comparison study was conducted. A total of 61 IBD patients from the Second Affiliated Hospital of Xi'an Jiaotong University who underwent simultaneous ELISA and FICA testing from May to June 2025 were prospectively enrolled. Using Best Crohn's disease activity index and modified Mayo score as gold standards, optimal FC cut-offs for assessing the disease activity were determined by receiver operating characteristic (ROC) curve analysis. Numerical consistency was evaluated via Spearman correlation, Passing-Bablok regression, and Bland-Altman analysis. Classification consistency was assessed by Cohen's Kappa coefficient based on both manufacturer-recommended cut-offs (ELISA: 200 μg/g, FICA: 100 μg/g) and ROC-optimized cut-offs.Results:Of the 61 patients, 28 were male and 33 were female, with a median age of 48 (34, 61) years and a disease duration of 48 (12, 109) months; 43 had ulcerative colitis (UC) and 18 had Crohn's disease (CD) ; 35 were in remission and 26 were in the active stage. Median FC concentrations were 178.0 (30.0, 1 342.0) μg/g by ELISA and 67.2 (15.0, 275.6) μg/g by FICA. The area under the curve (AUC) for ELISA in diagnosing activity of IBD was 0.930, with a sensitivity of 80.0% and specificity of 96.2% at the optimal cut-off of 154.0 μg/g. The AUC for FICA was 0.784, with a sensitivity of 80.0% and specificity of 80.8% at the optimal cut-off of 81.2 μg/g. DeLong test showed that the overall diagnostic efficacy of ELISA was significantly superior to that of FICA ( Z = 2.550, P = 0.011). Spearman correlation analysis showed a correlation coefficient of 0.62 (95% CI: 0.41-0.73, P < 0.001) between ELISA and FICA results. The Cusum linearity test indicated a linear relationship between the two methods ( P = 0.291). Passing-Bablok regression yielded the equation y = -53.38 + 5.56x, indicating both significant constant and proportional systematic errors between ELISA and FICA, and the errors increased with the concentrations. Bland-Altman analysis demonstrated ELISA assay values were systematically higher than those of FICA (overall mean bias: 74.5%, 95% limits of agreement: -101.0% to 250.0%), with larger differences in active disease than remission (the mean bias: 100.4% vs. 48.0%). Classification consistency improved markedly when using ROC-optimized cut-offs compared with manufacturer-recommended cut-offs (Kappa: 0.608 vs. 0.474) . Conclusions:Both ELISA and FICA can effectively identify active IBD but exhibit concentration-dependent systematic bias (ELISA > FICA). The consistent use of a single assay is recommended for disease monitoring and ROC-optimized cut-offs are adopted to improve the accuracy of disease activity stratification.

Objective To investigate the multi spiral CT findings of ovarian endometrioid adenofibroma.Methods The clinical and CT imaging data of 7 patients with ovarian endometrioid adenofibroma were retrospectively analyzed,and the characteristic CT findings were summarized.Results All 7 patients of ovarian endometrioid adenofibroma in this group were unilateral,with tumors presenting as round or lobulated masses.Most cases were cystic-solid lesions.The density of cystic fluid was higher than that of water or urine,and fibrous septa of varying thickness were visible.Enhanced scanning showed solid part and fibrous septa with moderate enhancement.Conclusion The CT findings of ovarian endometrioid adenofibroma have certain characteristics,measuring the density of cystic fluid and enhancing scanning can improve it's diagnostic accuracy.

Objective:To compare the diagnostic efficacy and consistency of fecal calprotectin (FC) detected by enzyme-linked immunosorbent assay (ELISA) and fluorescence immunochromatography assay (FICA) in assessing the disease activity of inflammatory bowel disease (IBD) .Methods:The paired-design diagnostic test comparison study was conducted. A total of 61 IBD patients from the Second Affiliated Hospital of Xi'an Jiaotong University who underwent simultaneous ELISA and FICA testing from May to June 2025 were prospectively enrolled. Using Best Crohn's disease activity index and modified Mayo score as gold standards, optimal FC cut-offs for assessing the disease activity were determined by receiver operating characteristic (ROC) curve analysis. Numerical consistency was evaluated via Spearman correlation, Passing-Bablok regression, and Bland-Altman analysis. Classification consistency was assessed by Cohen's Kappa coefficient based on both manufacturer-recommended cut-offs (ELISA: 200 μg/g, FICA: 100 μg/g) and ROC-optimized cut-offs.Results:Of the 61 patients, 28 were male and 33 were female, with a median age of 48 (34, 61) years and a disease duration of 48 (12, 109) months; 43 had ulcerative colitis (UC) and 18 had Crohn's disease (CD) ; 35 were in remission and 26 were in the active stage. Median FC concentrations were 178.0 (30.0, 1 342.0) μg/g by ELISA and 67.2 (15.0, 275.6) μg/g by FICA. The area under the curve (AUC) for ELISA in diagnosing activity of IBD was 0.930, with a sensitivity of 80.0% and specificity of 96.2% at the optimal cut-off of 154.0 μg/g. The AUC for FICA was 0.784, with a sensitivity of 80.0% and specificity of 80.8% at the optimal cut-off of 81.2 μg/g. DeLong test showed that the overall diagnostic efficacy of ELISA was significantly superior to that of FICA ( Z = 2.550, P = 0.011). Spearman correlation analysis showed a correlation coefficient of 0.62 (95% CI: 0.41-0.73, P < 0.001) between ELISA and FICA results. The Cusum linearity test indicated a linear relationship between the two methods ( P = 0.291). Passing-Bablok regression yielded the equation y = -53.38 + 5.56x, indicating both significant constant and proportional systematic errors between ELISA and FICA, and the errors increased with the concentrations. Bland-Altman analysis demonstrated ELISA assay values were systematically higher than those of FICA (overall mean bias: 74.5%, 95% limits of agreement: -101.0% to 250.0%), with larger differences in active disease than remission (the mean bias: 100.4% vs. 48.0%). Classification consistency improved markedly when using ROC-optimized cut-offs compared with manufacturer-recommended cut-offs (Kappa: 0.608 vs. 0.474) . Conclusions:Both ELISA and FICA can effectively identify active IBD but exhibit concentration-dependent systematic bias (ELISA > FICA). The consistent use of a single assay is recommended for disease monitoring and ROC-optimized cut-offs are adopted to improve the accuracy of disease activity stratification.

Objective To investigate the multi spiral CT findings of ovarian endometrioid adenofibroma.Methods The clinical and CT imaging data of 7 patients with ovarian endometrioid adenofibroma were retrospectively analyzed,and the characteristic CT findings were summarized.Results All 7 patients of ovarian endometrioid adenofibroma in this group were unilateral,with tumors presenting as round or lobulated masses.Most cases were cystic-solid lesions.The density of cystic fluid was higher than that of water or urine,and fibrous septa of varying thickness were visible.Enhanced scanning showed solid part and fibrous septa with moderate enhancement.Conclusion The CT findings of ovarian endometrioid adenofibroma have certain characteristics,measuring the density of cystic fluid and enhancing scanning can improve it's diagnostic accuracy.

Objective:To investigate the effect of a degradable high-purity magnesium screw in fixing the greater trochanter bone flap of a lateral circumflex femoral artery transverse branch in the treatment of ischemic necrosis of femoral head in young and middle-aged adults.Methods:From February 2017 to February 2019, 12 cases (15 hips) of young and middle-aged patients with avascular necrosis of femoral head were treated in the Department of Orthopaedic of Affiliated Zhongshan Hospital of Dalian University. The age of patients was 30-53 years old. According to Association Research Circulation Osseous (ARCO), 2 hips were graded in stage II b, 4 in ARCO II c, 1 in ARCO III a, 5 in ARCO III b, 2 in ARCO III c and 1 in ARCO IV. The greater trochanter bone flap with a lateral circumferential vascular branch was used to fill the necrotic area, and fixed by a biodegradable high purity magnesium screw in the bone flap transfer. At 3, 6 and 12 months postoperation, the patient came to the hospital outpatient clinic for follow-up, and then were reviewed once a year. Imaging efficacy was evaluated by comparing preoperative and postoperative imaging. The Harris score and Visual Anoalogue Scale (VAS) score were tested at 12 and 24 months after surgery. The Harris score and VAS score before and after surgery were compared by Friedman test, and P<0.05 was considered statistically significant. Results:All 12 patients (15 hips) were entered in the 24-36 months of follow-up. At 12 and 24 months after surgery, Harris score was found at 87 (86, 92) and 90 (87, 92) respertively, which were both higher than that before surgery [59 (52, 74)] with a significant statistical difference ( Z=-3.743, Z=-4.473, P<0.05). However, there was no significant difference in Harris scores between 12 and 24 months after the surgery ( Z=-0.730, P>0.05). At the 12 and 24 months after surgery, VAS score was found at 3 (2, 3) and 2 (1, 3) respertively, which were both lower than that before surgery [6 (5, 6) ] with a significant statistical difference ( Z=-3.560, Z=-4.656, P<0.05). There was no statistical difference in VAS scores between 12 and 24 months after surgery ( Z=-1.095, P>0.05). X-ray and CT scan showed that the bone flaps healed well and the areas of osteonecrosis were repaired. Thirteen femoral heads were in good shape, and 2 femoral heads had further collapse of hips. No patients underwent joint replacement surgery at the time of last follow-up. Conclusion:Fixation of the greater trochanter flap of lateral circumflex femoral artery transverse branch with a degradable high-purity magnesium screw can ensure the healing of the flap at the implantation site and avoid the displacement and shedding of the flap. It is a new therapeutic option to treat the avascular necrosis of femoral head of young and middle-aged people.

The subpulmonary ventricular exclusion (Fontan) could effectively improve the living quality for the children patients with a functional single ventricle in clinical. However, postoperative Fontan circulation failure can easily occur, causing obvious limitations while clinically implementing Fontan. The cavopulmonary assist devices (CPAD) is currently an effective means to solve such limitations. Therefore, in this paper the
Algorithms ; Child ; Feedback ; Heart-Assist Devices ; Hemodynamics ; Humans ; Models, Cardiovascular

Tooth loss is a common disease in the elderly, and periodontitis is the main cause of tooth loss. Alzheimer′s disease is a primary degenerative brain disease which etiology remains unknown. The patients often demonstrate cognitive impairment with characteristic neuropathological and neurochemical changes. The present article reviewed the relationship and associated mechanisms between tooth loss and Alzheimer′s disease.

The ethics review for clinical trials pertaining to human body highly emphasizes human subjects protection.This study discussed how to balance the relationship between the rights,safety and health of the subjects and interests of the science and the community,how to review the damage compensation provisions and insurance provisions in the informed consent,how to scientifically define the human subject biomedical trials involving vulnerable groups,and how to conduct trials on vulnerable groups,as well as other sensitive issues in ethics review.

Objective To investigate the effects of hyperbaric oxygen (HBO) combined with neural stem cell (NSC) transplantation on functional recovery after spinal cord injury (SCI).Methods Forty healthy adult Sprague-Dawley rats were randomly divided into a sham group,an SCI group,an NSC group and an HBO+NSC group.In the latter three groups,complete spinal cord transection was performed at T10.The rats of the HBO+NSC group were treated with HBO therapy after NSC transplantation.Hindlimb motor function was assessed with the BassoBeattie-Bresnahan (BBB) scale and tilt table testing.Motor evoked potentials (MEPs) were measured to evaluate the rats'nerve conduction function.The amount of surviving transplanted NSCs at the injured site was counted using Hoechst dyeing.Results The average BBB scores and tilt table test results in the HBO+NSCs group were significantly better than in the SCI and NSC groups from the 2nd week post-SCI.By the 4th week the average latency period and MEP amplitude in the HBO+NSC group were significantly better than in the NSC group,while MEPs in the SCI group still had not re-appeared.The surviving amount of transplanted NSCs in the HBO+NSC group was also significantly greater than in the NSC group.Conclusion HBO combined with NSC transplantation has synergistic effects and can improve functional recovery after SCI.This may be explained by the important role of HBO in promoting the survival of transplanted NSCs.

The ensemble empirical mode decomposition (EEMD) can be used to overcome the mode mixing problem of empirical mode decomposition (EMD) effectively. The EEMD method and Hilbert-Huang Transform (HHT) can be used to analyze pulse signals of Traditional Chinese Medicine (TCM). The amplitudes of the added white noise were about 0.1 and 0.2 time standard deviation of the investigated signal respectively. The difference of average frequency and average energy of every mode between normal pulse, slippery pulse, wiry pulse and wiry-slippery pulse were demonstrated based on different amplitudes of the added white noise. The results showed that it is more in line with clinical practice when the amplitude of the added white noise is about 0.2 time standard deviation of the investigated signal.
Algorithms ; Artifacts ; Diagnosis, Differential ; Humans ; Medicine, Chinese Traditional ; methods ; Pulse ; Signal Processing, Computer-Assisted