Objective:To develop and validate artificial intelligence (AI) models based on deep learning for precise boundary identification of early gastric cancer (EGC) in narrow-band imaging (NBI) and near focus narrow-band imaging (NF-NBI) endoscopic images.Methods:Endoscopic submucosal dissection (ESD) images from 282 patients diagnosed as having EGC by postoperative pathology at the Department of Gastroenterology, the First Affiliated Hospital of Soochow University were retrospectively collected from February 2016 to June 2024. The images were randomly divided into the training set and the validation set at an approximate 8∶2 ratio. In the NBI modality, 980 images from 171 patients were used for training, 235 images from 61 patients were used for validation. In the NF-NBI modality, 1 273 images from 128 patients were used for training, and 373 images from 35 patients were used for validation. This study trained a total of six convolutional neural network (CNN) models: two independent CNN1 models, two independent CNN2 models, and two fused CNN3 models. Using expert-delineated EGC boundaries based on post-ESD pathological findings as the gold standard, the intersection over union (IOU) value of the CNN3 models was compared against junior (<5 years experience), mid-level (5-10 years), and senior (>10 years) endoscopists.Results:In NBI validation set, the IOU value of CNN3 model was significantly higher than that of junior (0.732 VS 0.489, Z=11.528, P<0.001) and mid-level endoscopists (0.732 VS 0.521, Z=11.184, P<0.001). However, no significant difference was observed between CNN3 model and senior endoscopists (0.732 VS 0.739, Z=0.593, P=0.554). Similarly, in NF-NBI validation set, CNN3 model outperformed junior (0.757 VS 0.537, Z=15.944, P<0.001) and mid-level endoscopists (0.757 VS 0.597, Z=9.722, P<0.001), while matching senior endoscopists (0.757 VS 0.769, Z=0.854, P=0.394). Conclusion:The fused CNN3 model achieves senior expert-level accuracy in delineating EGC boundaries in both NBI and NF-NBI images, demonstrating potential to assist less-experienced endoscopists in precise identification of EGC boundaries.

Objective:To develop and validate artificial intelligence (AI) models based on deep learning for precise boundary identification of early gastric cancer (EGC) in narrow-band imaging (NBI) and near focus narrow-band imaging (NF-NBI) endoscopic images.Methods:Endoscopic submucosal dissection (ESD) images from 282 patients diagnosed as having EGC by postoperative pathology at the Department of Gastroenterology, the First Affiliated Hospital of Soochow University were retrospectively collected from February 2016 to June 2024. The images were randomly divided into the training set and the validation set at an approximate 8∶2 ratio. In the NBI modality, 980 images from 171 patients were used for training, 235 images from 61 patients were used for validation. In the NF-NBI modality, 1 273 images from 128 patients were used for training, and 373 images from 35 patients were used for validation. This study trained a total of six convolutional neural network (CNN) models: two independent CNN1 models, two independent CNN2 models, and two fused CNN3 models. Using expert-delineated EGC boundaries based on post-ESD pathological findings as the gold standard, the intersection over union (IOU) value of the CNN3 models was compared against junior (<5 years experience), mid-level (5-10 years), and senior (>10 years) endoscopists.Results:In NBI validation set, the IOU value of CNN3 model was significantly higher than that of junior (0.732 VS 0.489, Z=11.528, P<0.001) and mid-level endoscopists (0.732 VS 0.521, Z=11.184, P<0.001). However, no significant difference was observed between CNN3 model and senior endoscopists (0.732 VS 0.739, Z=0.593, P=0.554). Similarly, in NF-NBI validation set, CNN3 model outperformed junior (0.757 VS 0.537, Z=15.944, P<0.001) and mid-level endoscopists (0.757 VS 0.597, Z=9.722, P<0.001), while matching senior endoscopists (0.757 VS 0.769, Z=0.854, P=0.394). Conclusion:The fused CNN3 model achieves senior expert-level accuracy in delineating EGC boundaries in both NBI and NF-NBI images, demonstrating potential to assist less-experienced endoscopists in precise identification of EGC boundaries.

Objective:To investigate the expression of annexin A2 pseudogene 1 (ANXA2P1) in glioblastoma (GBM) tissues and its biological function in U87 cells.Methods:The expression level of ANXA2P1 in GBM tissues was analyzed by Cancer Genome Atlas (TCGA). Cell transfection was used to up-regulate or down-regulate the expression of ANXA2P1 in GBM tissues or U87 cells. Cell Counting Kit-8 (CCK-8) was used to detect the proliferation ability of U87 cells. Western blotting was used to detect the changes of protein expression in signaling pathway of U87 cells.Results:The TCGA analysis showed that the expression level of ANXA2P1 in high-level GBM tissues was significantly higher than that in low-level GBM tissues ( P<0.01). In GBM tissues, the expression level of ANXA2 was positively correlated with that of ANXA2P1 ( r=0.752, P<0.01). The proliferation capacity of U87 cells with ANXA2P1 overexpression was higher than that of the control group, while the proliferation capacity of U87 cells transfected by Si-ANXA2P1 was lower than that of the control group. At 72 h after transfection, the expression level of p-S6K protein in the ANXA2PI-overexpressed cells was higher than that in the control group ( P<0.05), while the expression level of p-S6K protein in the Si-ANXA2P1 cells was lower than that in the control group ( P<0.05). Conclusion:ANXA2P1 may promote the proliferation of U87 cells through mTOR signaling pathway.

Objective:To investigate the expression of annexin A2 pseudogene 1 (ANXA2P1) in glioblastoma (GBM) tissues and its biological function in U87 cells.Methods:The expression level of ANXA2P1 in GBM tissues was analyzed by Cancer Genome Atlas (TCGA). Cell transfection was used to up-regulate or down-regulate the expression of ANXA2P1 in GBM tissues or U87 cells. Cell Counting Kit-8 (CCK-8) was used to detect the proliferation ability of U87 cells. Western blotting was used to detect the changes of protein expression in signaling pathway of U87 cells.Results:The TCGA analysis showed that the expression level of ANXA2P1 in high-level GBM tissues was significantly higher than that in low-level GBM tissues ( P<0.01). In GBM tissues, the expression level of ANXA2 was positively correlated with that of ANXA2P1 ( r=0.752, P<0.01). The proliferation capacity of U87 cells with ANXA2P1 overexpression was higher than that of the control group, while the proliferation capacity of U87 cells transfected by Si-ANXA2P1 was lower than that of the control group. At 72 h after transfection, the expression level of p-S6K protein in the ANXA2PI-overexpressed cells was higher than that in the control group ( P<0.05), while the expression level of p-S6K protein in the Si-ANXA2P1 cells was lower than that in the control group ( P<0.05). Conclusion:ANXA2P1 may promote the proliferation of U87 cells through mTOR signaling pathway.

Objective To investigate the intervention of chemokine receptor 4(CXCR4) antagonist AMD3100 in lung tissues of rats during pulmonary oxygen intoxication.Methods Forty SD rats were randomly divided into 4 groups:normal pressure air PBS group, normal pressure air antagonist group , oxygen exposure PBS group and oxygen exposure antagonist group, each consisting of 10 animals.The last two groups were compressed to 0.23 MPa at an exponential rate of 0.1 MPa/min by pure oxygen.Pathological changes of lung tissues were observed by hematoxylin eosin stain.Changes in TNF-αand IL-1βexpression levels in the lung tissues of rats were detected by ELISA.Changes in CXCR4 expression levels were ob-served by Western blotting.Results Pathological examination indicated that edema and hemorrhage in the alveolar and pulmonary interstitial tissue of oxygen exposure antagonist group were lighter than in oxygen exposure PBS group.The levels of TNF-α, IL-1βand cleaved-caspase-3 in the lung tissues of the oxygen exposure antagonist group were lower than in oxy-gen exposure PBS group.Conclusion Blocking CXCR4 with AMD3100 can effectively alleviate lung injury during pulmo-nary oxygen intoxication.

Objective:To explore the clinical value of 16-slice spiral CT in diagnosing bony lacrimal passage fracture.Methods:16-slice spiral CT images of 65 patients,which were clinically proved as bony lacrimal passage fractures,were analyzed retro-spectively.Postprocessing of images was performed by GE AW 4.1.Multi planar reconstruction(MPR).The thin-thick axial images were compared with the MPR.Results:16-slice spiral CT showed clearly the fractures of lacrimal sac fossa and nasolac-rimal duct.All the 65 cases were compound fracture,most of which were naso-orbital-ethmoid fracture,including 45 cases of fractures of the frontal process of the maxilla,30 cases of the lacrimal bone fractures,40 cases of ethmoid bone fractures on the medial orbital wall,32 cases of nasal bone fractures,13 cases of the nasal process fractures of frontal bone,23 cases with naso-maxillary suture separations,17 cases with naso-frontal suture separations,20 cases with frontal-maxillary suture separations, 50 cases with lacrimo-maxillary suture separations,26 cases with internasal suture separations.There was no significant difference between the thin-thick axial images and the MPR in diagnosing bony lacrimal passage fracture.Conclusions:16-slice spiral CT can accurately display the location,extent and secondary changes of bony lacrimal passage fracture in detail.

Objective To study the change pattern for the expression of chemokine receptor 4 (CXCR4) in the lung tissue of rats with pulmonary oxygen toxicity.Methods Thirty SD rats were randomly divided into 6 groups:the control group,the 2 h pure oxygen exposure group (or simply the 2 h group),the 4 h pure oxygen exposure group (the 4 h group),the 6 h group,the 8 h group and the 10 h group,each consisting of 5 animals.Observations on pathological changes in the lung tissue and changes in the CXCR4 expression levels in the lung tissues of rats were performed by Western Blot,then,comparisons were made with those of the control group.Results Pathological detection indicated that stasis of blood,edema and hemorrhage in the alveolar and pulmonary interstitial tissue deteriorated with the extension of oxygen exposure time.The expression level of CXCR4 in the lung tissue increased with the extension of exposure within 8 hours,however,the levels of CXCR4 in the 10 h group decreased to some extent,as compared with that of the 8 h group.Conclusions Mter exposure to high pressure oxygen,lung damage occurred in pulmonary tissue of rats,with the change pattern basically identical to that of CXCR4.

Objective To study the change pattern for the expression of chemokine receptor 4 (CXCR4) in the lung tissue of rats with pulmonary oxygen toxicity.Methods Thirty SD rats were randomly divided into 6 groups:the control group,the 2 h pure oxygen exposure group (or simply the 2 h group),the 4 h pure oxygen exposure group (the 4 h group),the 6 h group,the 8 h group and the 10 h group,each consisting of 5 animals.Observations on pathological changes in the lung tissue and changes in the CXCR4 expression levels in the lung tissues of rats were performed by Western Blot,then,comparisons were made with those of the control group.Results Pathological detection indicated that stasis of blood,edema and hemorrhage in the alveolar and pulmonary interstitial tissue deteriorated with the extension of oxygen exposure time.The expression level of CXCR4 in the lung tissue increased with the extension of exposure within 8 hours,however,the levels of CXCR4 in the 10 h group decreased to some extent,as compared with that of the 8 h group.Conclusions Mter exposure to high pressure oxygen,lung damage occurred in pulmonary tissue of rats,with the change pattern basically identical to that of CXCR4.

OBJECTIVE: To improve diagnosis and treatment level of foreign body ingestion of button cell in children. METHOD: Among the 14 cases with foreign bodies ingestion of button cell, 6 cases in esophageal, 7 cases in stomach and lower gastrointestinal tract, 1 case with multiple foreign bodies both in esophagus and stomach. Seven cases in stomach and lower gastrointestinal tract were closely observed without special treatment, 6 cases in esophageal, underwent esophagoscopy and removal of foreign body under general anesthesia, 1 case with multiple foreign bodies in esophagus and stomach underwent esophagoscopy and electronic gastroscopy and then removal of foreign body. All patients had symptomatic treatments after removal of foreign body. The average age is 2 years and 1 month. RESULT: After the removal of button cell, Foreign bodies in stomach and lower gastrointestinal tract in 7 cases were all discharged spontaneously. For the 6 cases in esophagus, 4 cases were cured, 1 case had esophageal stricture, 1 cased died. 1 case with multiple foreign bodies in both esophagus and stomach was cured. CONCLUSION Esophageal foreign body has strong corrosiveness and would cause serious damages even to threaten children's life. It also may lead to esophageal stricture. The key point to cure this disease is to diagnose timely and to operate as soon as possible. Because neutralize of digestive juices and low probability of incarceration, with close observation, the foreign bodies of stomach and lower gastrointestinal tract can discharge spontaneously.
Child, Preschool ; Esophagus ; Female ; Foreign Bodies ; etiology ; Humans ; Infant ; Male ; Stomach

OBJECTIVE: To improve diagnosis and treatment level of esophageal foreign bodies of button cell. METHOD: Reported 4 clinical cases of esophageal foreign bodies of button cell. RESULT: Because of different diagnosis time and different surgical time, one case died and the other were cured. CONCLUSION Esophageal foreign bodies of button cell has been reported rarely before. Because button cell is caustically basic, drastically dangerous, it should be emphasized clinically. The key point to cure this disease is diagnosing timely and operating a surgery as soon as possible.
Esophagus ; Female ; Foreign Bodies ; etiology ; therapy ; Humans ; Infant ; Male