1.Biomechanical Evaluation of Flexor Tendon Repair: Double Loop Technique with Epitendinous Suture
Sadagatullah AN ; Raghu S ; Paiman M ; Ismail S ; Jusoh MH
Malaysian Orthopaedic Journal 2025;19(No. 2):1-8
Introduction: There are various methods used to repair
lacerated tendons. The minimum requirement for the best
results and lowest rupture rate is the four-strand repair
technique. The cruciate type of repair is among the most
popular methods available but is very technical and requires
expertise. An easier two-double-loop method for tendon
healing is suggested in this study. This study assessed the
biomechanical properties of two well-known tendon repair
techniques—the modified Kessler and cruciate
approaches—and two lesser-known double-loop techniques
for tensile strength, stiffness, and failure mode.
Materials and methods: Twenty-four adult chickens'
Achilles tendons were randomly divided into three groups
and sutured with a four-strand core suture using the fourstrand modified Kessler technique, the four-strand cruciate
technique, and the two-double-loop approach. Twenty-four
more adult chicken Achilles tendons were acquired, and they
were randomly assigned to the same three groups along with
an extra running epitendinous repair. A synthetic, nonabsorbable monofilament polypropylene suture was used for
all repairs.
Results: The four-strand modified Kessler, and the fourstrand cruciate procedures had the lowest mean ultimate
tensile strength, whereas the two double-loop techniques had
the strongest. The results were dramatically impacted by
using an epitendinous suture during test analysis.
Conclusion: The strongest and comparatively less
technically complex technique used in this investigation was
the two-double-loop, four-strand core suture method. The
significance of the extra strength that the epitendinous suture
gave was clear. Using this in a clinical setting is
recommended for hand flexor tendon injuries.
2.Presence of SARS-CoV-2-like coronaviruses in bats from east coast Malaysia
Gilbert, M. ; Mohamed, M. ; Choong, S.S. ; Baqi, A. ; Kumaran, J.V. ; Sani, I. ; Noralidin, A ; Manaf, A. ; Reduan, F.H. ; Tan, L.P. ; Jusoh, M.
Tropical Biomedicine 2023;40(No.3):273-280
Most of the public health importance coronaviruses, such as Severe acute respiratory syndrome
coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV) and SARS-CoV-2
are likely originated from bats and spread to humans through intermediate hosts; civet cats, dromedary
camel and Malayan pangolin, respectively. SARS-CoV-2-like coronaviruses were detected in Thailand,
which is neighbouring with Kelantan in East Coast Malaysia. To date, there is no report on the presence
of public health concerns (SARS-CoV, SARS-CoV-2 and MERS-CoV) coronaviruses in bats from Malaysia.
This study was aimed to elucidate the presence of these coronaviruses in bat samples from East Coast,
Malaysia. A total of hundred seventy oropharyngeal swab samples were collected from three states
of East Coast Malaysia. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was conducted
based on partial 3’ Untranslated region (3’UTR) or ORF10 gene and the products were sequenced. The
sequences were compared with all coronavirus sequences from the National Center for Biotechnology
Information-GenBank (NCBI-GenBank) using NCBI-Basic Local Alignment Search Tool (NCBI-BLAST)
software. A phylogenetic tree was constructed to determine the genetic relationship among the detected
coronaviruses with the reference coronaviruses from the NCBI-GenBank. Our results showed that SARSCoV-2-like viruses were present in 3% (5/170) of the bats from East Coast Malaysia that have 98-99%
sequence identities and are genetically related to SARS-CoV-2 from humans. This finding indicates the
presence of SARS-CoV-2-like viruses in bats from East Coast Malaysia that may become a public health
concern in the future.


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