The production of high-quality oocytes requires precisely orchestrated intercellular communication. Extracellular vesicles (EVs) are cell-derived nanoparticles that play a vital role in the transfer of bioactive molecules, which has gained much attention in the field of diagnosis and treatment. Over the past ten years, the participation of EVs in the reproductive processes of oocytes has been broadly studied and has shown great potential for elucidating the intricacies of female reproductive health. This review provides an extensive discussion of the influence of EVs on oocytes, emphasizing their involvement in normal physiology and altered cargo under pathological conditions. In addition, the positive impact of therapeutic EVs on oocyte quality and their role in alleviating ovarian pathological conditions are summarized.
Humans ; Extracellular Vesicles/physiology* ; Oocytes/cytology* ; Female ; Animals ; Cell Communication/physiology*

Objective On the basis of the"dual improvement and dual enhancement"of medical services,analyze the results of the patient satisfaction evaluation questionnaire survey report,in order to provide suggestions and reference value for hospital satisfaction management work,improve patient satisfaction in hospitals,and enhance the medical experience.Methods Analyze the hospital management situation based on the results of 460 reports from a satisfaction questionnaire survey conducted in the third quarter of 2024 at a certain hospital.Results The overall satisfaction score of patients in the third quarter of 2024 was 92.26 points;Patients have higher satisfaction with the convenient process and doctors,but lower satisfaction with the envi-ronment and medical technology;Patients have higher satisfaction with nursing and doctors,but lower satisfaction with the envi-ronment and cafeteria.The main problems in hospitals are outpatient medical technology services,inpatient environment,and doctor-patient communication.0.77% of patients believe that hospitals need to strengthen convenient facilities,and 8.46% of patients believe that environmental sanitation needs to be strengthened.Conclusion Hospitals need to further improve the pa-tient's medical environment,optimize the examination process,shorten the waiting time for examinations,enhance the quality of medical technology,improve the quality of medical services,strengthen the notification of precautions,and promote harmonious doctor-patient relationships,in order to comprehensively achieve the"dual improvement"plan,improve patient satisfaction,and enhance the medical experience.

Objective On the basis of the"dual improvement and dual enhancement"of medical services,analyze the results of the patient satisfaction evaluation questionnaire survey report,in order to provide suggestions and reference value for hospital satisfaction management work,improve patient satisfaction in hospitals,and enhance the medical experience.Methods Analyze the hospital management situation based on the results of 460 reports from a satisfaction questionnaire survey conducted in the third quarter of 2024 at a certain hospital.Results The overall satisfaction score of patients in the third quarter of 2024 was 92.26 points;Patients have higher satisfaction with the convenient process and doctors,but lower satisfaction with the envi-ronment and medical technology;Patients have higher satisfaction with nursing and doctors,but lower satisfaction with the envi-ronment and cafeteria.The main problems in hospitals are outpatient medical technology services,inpatient environment,and doctor-patient communication.0.77% of patients believe that hospitals need to strengthen convenient facilities,and 8.46% of patients believe that environmental sanitation needs to be strengthened.Conclusion Hospitals need to further improve the pa-tient's medical environment,optimize the examination process,shorten the waiting time for examinations,enhance the quality of medical technology,improve the quality of medical services,strengthen the notification of precautions,and promote harmonious doctor-patient relationships,in order to comprehensively achieve the"dual improvement"plan,improve patient satisfaction,and enhance the medical experience.

Recombinant human interferon α2b(rhIFNα2b)is widely used as an antiviral therapy agent for the treatment of hepatitis B and hepatitis C.The current identification test for rhIFNα2b is complex.In this study,an anti-rhIFNα2b nanobody was discovered and used for the development of a rapid lateral flow strip for the identification of rhIFNα2b.RhIFNα2b was used to immunize an alpaca,which established a phage nanobody library.After five steps of enrichment,the nanobody I22,which specifically bound rhIFNα2b,was isolated and inserted into the prokaryotic expression vector pET28a.After subsequent purification,the physicochemical properties of the nanobody were determined.A semiquantitative detection and rapid identification assay of rhIFNα2b was developed using this novel nanobody.To develop a rapid test,the nanobody I22 was coupled with a colloidal gold to produce lateral-flow test strips.The developed rhIFNα2b detection assay had a limit of detection of 1 μg/mL.The isolation of I22 and successful construction of a lateral-flow immunochromatographic test strip demonstrated the feasibility of performing ligand-binding assays on a lateral-flow test strip using recombinant protein products.The principle of this novel assay is generally applicable for the rapid testing of other com-mercial products,with a great potential for routine use in detecting counterfeit recombinant protein products.

This article reports a case of severe ulcerative colitis (SUC) . On the basis of excluding the opportunistic infection, the patients failed in hormone therapy with following intestinal perforation, and received emergency surgery finally. After the surgery, the main treatment discussed by the cooperation of multidisciplinary team (MDT) was enteral nutritional support therapy with rectal anti-inflammatory therapy as adjunctive treatment, and ileal pouch anal anastomosis was performed at selected time. The patient achieved a good quality of life. The case analysis combined with literatures was conducted in order to explore the MDT diagnosis and treatment strategies of SUC.

Cronkhite-Canada syndrome (CCS) is a rare disease characterized by diffuse gastrointestinal polyps, hair and nail loss and skin pigmentation. Its pathogenesis is unknown. This article reports a case of CCS, who was effectively treated with glucocorticoid and the gene mutation of colonic adenomatous polyp was detected by genetic testing, in order to provide reference for clinical diagnosis and treatment.

This article reports a case of severe ulcerative colitis (SUC) . On the basis of excluding the opportunistic infection, the patients failed in hormone therapy with following intestinal perforation, and received emergency surgery finally. After the surgery, the main treatment discussed by the cooperation of multidisciplinary team (MDT) was enteral nutritional support therapy with rectal anti-inflammatory therapy as adjunctive treatment, and ileal pouch anal anastomosis was performed at selected time. The patient achieved a good quality of life. The case analysis combined with literatures was conducted in order to explore the MDT diagnosis and treatment strategies of SUC.

Cronkhite-Canada syndrome (CCS) is a rare disease characterized by diffuse gastrointestinal polyps, hair and nail loss and skin pigmentation. Its pathogenesis is unknown. This article reports a case of CCS, who was effectively treated with glucocorticoid and the gene mutation of colonic adenomatous polyp was detected by genetic testing, in order to provide reference for clinical diagnosis and treatment.

Objective To investigate the expression of unfolded protein response (UPR) gene glucose regulated protein 78(GRP78) and X-box binding protein 1(XBP1) in esophageal squamous cell carcinoma, its effect on activation of the endoplasmic reticulum stress (ERS) and its mechanism in the growth of esophageal squamous cell carcinoma. Methods The expressions of GRP78 and XBP1 were detected by immunohistochemistry in 30 samples of esophageal squamous cell carcinoma and 30 samples of normal esophageal squamous epithelium. The correlation between expressions of both proteins and prognosis was analyzed. Results GRP78 positive rate was 83.3 %(25/30) in esophageal carcinoma, while the proportion was 20.0 %(6/30) in normal esophageal (χ2=25.833, P<0.05). XBP1 positive rate was 70.0 % (21/30) in esophageal carcinoma, while the proportion was 26.7%(8/30) in normal esophageal(χ2=20.872, P<0.05). The positive rates of GRP78 and XBP1 in invasive muscular layer of esophageal squamous cell carcinoma were significantly higher than those in invasive mucous layer. Conclusion GRP78 and XBP1 are highly expressed in esophageal squamous cell carcinoma, which may involve the occurrence and development of the esophageal carcinoma.

OBJECTIVETo investigate the protective effect of sesamin against cadmium chloride (CdCl2)-induced cardiotoxicity in rats.

METHODSFifty male Wistar rats were randomly assigned to five groups: control group, CdCl2 group, and low-, middle-, and high-dose sesamin groups. The control group was given normal saline. The CdCl2 group and sesamin groups were intraperitoneally injected with CdCl2 (5 mg/kg×2 d), and the low-, middle-, and high-dose sesamin groups were given 20, 40, and 80 mg/kg sesamin, respectively. All treatments lasted for four weeks. ECG was measured by a physiological recorder, and serum myocardial enzyme levels were determined by biochemical assay. The heart was weighed, and heart tissues were used in histopathological examination and determination of malondialdehyde (MDA) level.

RESULTSCompared with the control group, the CdCl2 group showed significantly higher levels of serum CK and CK-MB, an increased heart coefficient, significant ST-segment elevation, and higher level of MDA in myocardial tissue (P < 0.05). Histopathological analysis showed edema of myocardial tissues and cells, myocardial fibers disorder, karyopyknosis, and uneven or deep staining of nuclear chromatin. Different doses of sesamin relieved the myocardial pathological changes induced by CdCl2, and high-dose sesamin was the most effective. The middle- and high-dose sesamin groups showed significantly reduced serum CK and CK-MB levels compared with the CdCl2 group (P < 0.05). The heart coefficient of the high-dose sesamin group (0.19±0.01%) was significantly lower than that of the CdCl2 group (0.21±0.01%) (P < 0.05). Myocardial MDA levels of the three sesamin groups (42.32±4.65, 36.71±5.34, and 33.12±4.62 nmol/mg pro, respectively) were all significantly lower than that of the CdCl2 group (55.87±3.65 nmol/mg pro) (P < 0.05).

CONCLUSIONSesamin can relieve myocardial injury induced by CdCl2, and one possible mechanism is the enhancement of antioxidant capacity of myocardial tissue.

Animals ; Cadmium Chloride ; toxicity ; Creatine Kinase, MB Form ; blood ; Dioxoles ; pharmacology ; Heart ; drug effects ; Lignans ; pharmacology ; Male ; Malondialdehyde ; metabolism ; Myocardium ; metabolism ; pathology ; Rats ; Rats, Wistar