Purpose This study aimed to investigate the expression,function,and molecular mechanisms of ZNF384 in esophageal squamous cell carcinoma(ESCC),as well as its role in tumor progression and chemoresistance.Methods The expression of ZNF384 in ESCC cell lines and tissues was assessed using RT-qPCR.Correlations with TNM stage,invasion depth,lymph node metastasis,and prognosis were evaluated.In vitro assays were performed to examine the effects of ZNF384 on ESCC cell proliferation,migration,invasion,and chemosensitivity.Dual-luciferase reporter assays were conducted to determine the interaction between ZNF384 and FZD3,and to assess the activation of the Wnt signaling pathway.Results ZNF384 expression was significantly upregulated in ESCC cell lines and tissues(P＜0.01).Elevated ZNF384 expression was associated with advanced TNM stage,greater invasion depth,lymph node metastasis,and poor prognosis(P＜0.05).Functional assays demonstrated that ZNF384 overexpression promo-ted ESCC cell proliferation,migration,and invasion(all P＜0.01),whereas ZNF384 knockdown inhibited these processes and enhanced chemosensitivity to cisplatin(all P＜0.01).Mechanistic studies showed that ZNF384 directly bound to the FZD3 promoter,upregulated FZD3 expression,and activated the Wnt signaling pathway(P＜0.05).Overexpression of FZD3 partially reversed the inhibitory effects of ZNF384 knockdown on cell malignancy and chemore-sistance(P＜0.05).Conclusion ZNF384 promotes ESCC progression and reduces chemosensitivity through activa-tion of the FZD3/Wnt signaling pathway,suggesting its potential as a therapeutic target in ESCC.

Purpose This study aimed to investigate the expression,function,and molecular mechanisms of ZNF384 in esophageal squamous cell carcinoma(ESCC),as well as its role in tumor progression and chemoresistance.Methods The expression of ZNF384 in ESCC cell lines and tissues was assessed using RT-qPCR.Correlations with TNM stage,invasion depth,lymph node metastasis,and prognosis were evaluated.In vitro assays were performed to examine the effects of ZNF384 on ESCC cell proliferation,migration,invasion,and chemosensitivity.Dual-luciferase reporter assays were conducted to determine the interaction between ZNF384 and FZD3,and to assess the activation of the Wnt signaling pathway.Results ZNF384 expression was significantly upregulated in ESCC cell lines and tissues(P＜0.01).Elevated ZNF384 expression was associated with advanced TNM stage,greater invasion depth,lymph node metastasis,and poor prognosis(P＜0.05).Functional assays demonstrated that ZNF384 overexpression promo-ted ESCC cell proliferation,migration,and invasion(all P＜0.01),whereas ZNF384 knockdown inhibited these processes and enhanced chemosensitivity to cisplatin(all P＜0.01).Mechanistic studies showed that ZNF384 directly bound to the FZD3 promoter,upregulated FZD3 expression,and activated the Wnt signaling pathway(P＜0.05).Overexpression of FZD3 partially reversed the inhibitory effects of ZNF384 knockdown on cell malignancy and chemore-sistance(P＜0.05).Conclusion ZNF384 promotes ESCC progression and reduces chemosensitivity through activa-tion of the FZD3/Wnt signaling pathway,suggesting its potential as a therapeutic target in ESCC.

Objective:To conduct visualization analysis,induction and summary for the study status,focus and trend in the dose measurement field of flash radiotherapy(Flash-RT)through bibliometric methods on the basis of the Web of Science Collection(WOS),and analyze the technical bottleneck of Flash-RT dose measurement.Methods:In the WOS database,the("FLASH radiotherapy"or"ultra high dose rate"or"FLASH irradiation")and TS=("dosimetric system"or"dosimeters"or"dosimetry")were used as the theme terms,and all relevant literatures about the study of Flash-RT dose measurement that were included in the Core Collection of WOS database were retrieved.The citation analysis function of WOS and Citespace software(Version 6.2)were used to conduct visualization analysis for the publication trends,sources and research focus of the included literature of Flash-RT dose measurement,and to draw corresponding visualization knowledge maps.Results:The literatures were mainly published during January 2015 and November 2023,and a total of 86 papers were included in the analysis by screening,which included 78 articles and 8 reviews.The top three countries in terms of publication volume were respectively the United States(38 papers),France(19 papers)and Italy(19 papers).The research focuses were respectively Cherenkov,radiotherapy,passive dosimeter,conventional radiation,laser particle acceleration,ionization chamber and proton therapy as cluster analysis.Currently,the study of Flash-RT dose measurement mainly focused on the relevant fields included Flash-RT radiation source,dose rate,and the equipment and method of dose measurement.Conclusion:Flash-RT is a new technology with a key breakthrough in the basic field of radiotherapy,and this technique faces many difficulties and challenges in the clinical translation process of this technique.With the continuous development of science and technique,and deepeningaccumulation of related researchclinical big data,the method and procedure of accurate and efficient dose measurement dosimetry methods and procedures will promote and realize the update and iteration of Flash-RT experiment research and device technique,which will successfully realize the clinical the broader application prospects of this technology.

In this article, we report a case of spontaneous ruptured pyomyoma during pregnancy with successful delivery after myomectomy. A 35-year-old pregnant female (27 weeks of gestation) presented with lower abdominal pain. The patient had a history of uterine fibroids. Ultrasound, computed tomography, and magnetic resonance imaging confirmed a spontaneous ruptured pyomyoma (maximum diameter: 12.6 cm). Myomectomy was performed, and her abdominal distension and pain were significantly improved within 1 day; the condition of the fetus was normal. One month after surgery, severe tenderness was detected in the lower right side of the uterus. Considering the higher risk of uterine rupture and associated complications, a cesarean section was performed. The mother and neonate were discharged 3 and 42 days after delivery, respectively, in good condition. Myomectomy for spontaneous ruptured pyomyoma during pregnancy may be feasible and extend gestational age to improve outcomes for the mother and neonate.

In this article, we report a case of spontaneous ruptured pyomyoma during pregnancy with successful delivery after myomectomy. A 35-year-old pregnant female (27 weeks of gestation) presented with lower abdominal pain. The patient had a history of uterine fibroids. Ultrasound, computed tomography, and magnetic resonance imaging confirmed a spontaneous ruptured pyomyoma (maximum diameter: 12.6 cm). Myomectomy was performed, and her abdominal distension and pain were significantly improved within 1 day; the condition of the fetus was normal. One month after surgery, severe tenderness was detected in the lower right side of the uterus. Considering the higher risk of uterine rupture and associated complications, a cesarean section was performed. The mother and neonate were discharged 3 and 42 days after delivery, respectively, in good condition. Myomectomy for spontaneous ruptured pyomyoma during pregnancy may be feasible and extend gestational age to improve outcomes for the mother and neonate.

Objective:To evaluate the safety and effectiveness of adventitial inversion technique for root repair in patients with acute type A aortic dissection(ATAAD).Methods:Between 2015 and 2018, ATAAD patients with dissected root and underwent open surgery were included. The exclusion criteria were as follows: previous root intervention, traumatic dissection and patient underwent root replacement(Bentall or David procedure). 490 ATAAD patients were included, 366(74.69%) male and 124(25.31%) female, aged(51.28±10.99) years(range 24-77 years). The clinical data were retrospectively analyzed with ANOVA/ nonparametric test and Chi- square test. Follow-up mortality and reoperation were displayed with Kaplan- Meier curve. Results:All patients were technically divided into three groups: adventitial inversion(A), direct suture(B) and Cabrol-shunt(C). The mean age in group A was(53.05±11.09) years, whereas worse cardiac and renal function occurred in group C. The mean duration of HCA, CPB and ACC were shortest, with a highest average of minimum rectal temperature during surgical interval in group A. Postoperative complications and early mortality were similar among groups. There were no significant differences of mid-term mortality and reoperation among these three techniques. Though no late reintervention for aortic root was found in both group A and B, the root diameter was more stable in group A during follow-up period[(33.14±3.74)mm vs.(34.51±3.83)mm vs.(33.89±3.89)mm, P=0.008]. Conclusion:Adventitial inversion technique is safe and effective for root repair in patients with ATAAD, achieving satisfactory short- and mid-term effects.

[摘 要] 目的：检测miR-452-5p在食管鳞状细胞癌（ESCC）中的表达，并探讨其异常表达对食管癌KYSE-150细胞增殖、侵袭能力和EMT进程的影响及其分子机制。方法：收集2012年3月至2015年12月在河北医科大学第四医院就诊的86名ESCC患者的癌组织样本和对应的癌旁组织，用qPCR法检测miR-452-5p及其他相关基因在ESCC组织和细胞中的表达；向KYSE-150细胞中分别转染miR-452-5p mimic或pcDNA3.1-SOX7构建过表达的细胞株。分析miR-452-5p表达与ESCC病理特征和患者5年OS的关系。用MTS、Tanswell法检测miR-452-5p过表达对食管癌KYSE-150细胞增殖、侵袭能力和EMT进程的影响；用双荧光素酶报告基因实验及TOP/FOP报告基因系统检测miR-452-5p与SRY盒转录因子（SOX7）3'UTR区的结合作用及对Wnt/β-catenin通路活化水平的影响。结果：miR-452-5p在ESCC组织中呈明显高表达（P<0.01），并与ESCC患者的淋巴结转移、TNM分期及5年OS密切相关（均P<0.01）。miR-452-5p过表达明显促进食管癌KYSE-150细胞的增殖、侵袭能力及EMT进程（P<0.05或P<0.01）。SOX7是miR-452-5p的直接靶基因，miR-452-5p通过对SOX7的负向调控影响了Wnt通路活化水平（P<0.05或P<0.01），同时，miR-452-5p表达也受Wnt通路活化水平的影响（P<0.05或P<0.01），其可能为Wnt通路下游靶基因。结论：miR-452-5p通过miR-452-5p/SOX7/Wnt/miR-452-5p正反馈环路提高Wnt/β-catenin通路活化水平，进而促进ESCC KYSE-150细胞的增殖、侵袭能力及EMT进程，miR-452-5p有望成为ESCC患者靶向治疗的潜在靶点及预后评估的新型分子标志物。

With the extensive application of highly sensitive genetic techniques in the field of prenatal diagnosis, prenatal chromosomal mosaicisms including true fetal mosaicisms and confined placental mosaicisms are frequently identified in clinical settings, and the diagnostic criteria and principle of genetic counseling and clinical management for such cases may vary significantly among healthcare centers across the country. This not only has brought challenges to laboratory technician, genetic counselor and fetal medicine doctor, but can also cause confusion and anxiety of the pregnant woman and their family members. In this regard, we have formulated a consensus over the prenatal diagnosis and genetic counseling for chromosomal mosaicisms with the aim to promote more accurate and rational evaluation for fetal chromosomal mosaicisms in prenatal clinics.
Consensus ; Female ; Genetic Counseling ; Humans ; Mosaicism ; Placenta ; Pregnancy ; Prenatal Diagnosis/methods*

Objective:To explore the effect of dibutyl phthalate (DBP) on the rat testis Leydig cell apoptosis by AMP activated protein kinase/mammalian rapamycin target protein (AMPK/mTOR) signaling pathway.Methods:Rats with reproductive function impairment were divided into model (DBP) group of 17 rats, model+AMPK inhibitor [DBP+compound C (CC)] group of 17 rats, model+AMPK agonist [DBP+metformin (MF)] group of 17 rats, DBP+AMPK inhibitor+agonist (DBP+CC+MF) group of 17 rats by body mass ranking grouping method. Another 11 rats were taken as the blank group. The blank group and DBP group were intraperitoneally injected with the same amount of normal saline, while DBP+CC group and DBP+MF group were intraperitoneally injected with 20 mg/kg CC and 200 mg/kg MF respectively, and DBP+CC+MF group was intraperitoneally injected with CC and MF once a day for 4 weeks. Luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone (T) were measured by radioimmunoassay. Sperm quality was analyzed by automatic sperm quality analysis system. Leydig cell apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and flow cytometry. The expressions of AMPK, mTOR, Caspase 3 mRNA and protein, p-AMPK and p-mTOR protein were detected by RT-PCR and Western blotting. Results:The serum level of FSH in DBP+MF group [(9.88±0.67) U/L] increased, while that in DBP+CC group [(6.82±0.60) U/L] decreased compared with DBP group [(9.07±0.52) U/L] (all P<0.001). The serum LH, T levels and sperm concentration, percentage of (a+b) grade sperm in DBP+MF group [(3.97±0.70) U/L, (2.96±0.11) mg/L, (13.15±2.63)×10 6/mL, (22.20±4.13)%], DBP+CC group [(6.52±0.71) U/L, (4.48±0.15) mg/L, (25.47±2.18)×10 6/mL, (45.60±4.78)%] increased compared with DBP group [(4.51±0.75) U/L, (3.25±0.11) mg/L, (16.46±3.40)×10 6/mL, (25.43±4.36)%] (DBP group vs. DBP+MF group PLH=0.038, the other all P<0.001). HE staining showed that the structure of testis in blank group was normal. In DBP group and DBP+CC+MF group, the epithelial cells of seminiferous tubules atrophied and twisted in irregular shape, and the disease became serious in DBP+MF group, and there were a lot of vacuoles around the nucleus. The number of apoptosis, p-AMPK/AMPK protein relative expression and Caspase 3 mRNA and protein relative expression of Leydig cells in DBP+MF group (286.60±30.17, 0.95±0.08, 2.17±0.18, 1.23±0.10) increased, and DBP+CC group (88.00±21.34, 0.42±0.04, 1.35±0.15, 0.54±0.06) decreased compared with those in DBP group (142.40±26.78, 0.70±0.07, 1.85±0.14, 0.80±0.09, all P<0.001). Compared with DBP group (0.45±0.06), the p-mTOR/mTOR of DBP+MF group (0.23±0.04) decreased, and the p-mTOR/mTOR of DBP+CC group (0.84±0.07) increased (all P<0.001). Conclusion:DBP can damage the reproductive system of rats and increase the apoptosis rate of Leydig cells, which may be related to AMPK activation and mTOR inhibition.

Objective:To explore the effect of dibutyl phthalate (DBP) on the rat testis Leydig cell apoptosis by AMP activated protein kinase/mammalian rapamycin target protein (AMPK/mTOR) signaling pathway.Methods:Rats with reproductive function impairment were divided into model (DBP) group of 17 rats, model+AMPK inhibitor [DBP+compound C (CC)] group of 17 rats, model+AMPK agonist [DBP+metformin (MF)] group of 17 rats, DBP+AMPK inhibitor+agonist (DBP+CC+MF) group of 17 rats by body mass ranking grouping method. Another 11 rats were taken as the blank group. The blank group and DBP group were intraperitoneally injected with the same amount of normal saline, while DBP+CC group and DBP+MF group were intraperitoneally injected with 20 mg/kg CC and 200 mg/kg MF respectively, and DBP+CC+MF group was intraperitoneally injected with CC and MF once a day for 4 weeks. Luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone (T) were measured by radioimmunoassay. Sperm quality was analyzed by automatic sperm quality analysis system. Leydig cell apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and flow cytometry. The expressions of AMPK, mTOR, Caspase 3 mRNA and protein, p-AMPK and p-mTOR protein were detected by RT-PCR and Western blotting. Results:The serum level of FSH in DBP+MF group [(9.88±0.67) U/L] increased, while that in DBP+CC group [(6.82±0.60) U/L] decreased compared with DBP group [(9.07±0.52) U/L] (all P<0.001). The serum LH, T levels and sperm concentration, percentage of (a+b) grade sperm in DBP+MF group [(3.97±0.70) U/L, (2.96±0.11) mg/L, (13.15±2.63)×10 6/mL, (22.20±4.13)%], DBP+CC group [(6.52±0.71) U/L, (4.48±0.15) mg/L, (25.47±2.18)×10 6/mL, (45.60±4.78)%] increased compared with DBP group [(4.51±0.75) U/L, (3.25±0.11) mg/L, (16.46±3.40)×10 6/mL, (25.43±4.36)%] (DBP group vs. DBP+MF group PLH=0.038, the other all P<0.001). HE staining showed that the structure of testis in blank group was normal. In DBP group and DBP+CC+MF group, the epithelial cells of seminiferous tubules atrophied and twisted in irregular shape, and the disease became serious in DBP+MF group, and there were a lot of vacuoles around the nucleus. The number of apoptosis, p-AMPK/AMPK protein relative expression and Caspase 3 mRNA and protein relative expression of Leydig cells in DBP+MF group (286.60±30.17, 0.95±0.08, 2.17±0.18, 1.23±0.10) increased, and DBP+CC group (88.00±21.34, 0.42±0.04, 1.35±0.15, 0.54±0.06) decreased compared with those in DBP group (142.40±26.78, 0.70±0.07, 1.85±0.14, 0.80±0.09, all P<0.001). Compared with DBP group (0.45±0.06), the p-mTOR/mTOR of DBP+MF group (0.23±0.04) decreased, and the p-mTOR/mTOR of DBP+CC group (0.84±0.07) increased (all P<0.001). Conclusion:DBP can damage the reproductive system of rats and increase the apoptosis rate of Leydig cells, which may be related to AMPK activation and mTOR inhibition.