Objective To analyze the trajectory of cardiovascular health score and its influencing factors in patients with CHD after PCI based on LCGM.Methods A total of 186 CHD patients undergoing PCI in our hospital from June 2021 to June 2023 were enrolled,and all of them re-ceived an assessment for cardiovascular health scores.LCGM analysis was performed on the change in their postoperative cardiovascular health scores.Multivariate logistic regression analysis was applied to identify the factors affecting the trajectory of the change in the scores of the patients.Results The cardiovascular health score was 6.34±1.38,6.76±1.44,7.49±1.85,and 8.06±2.07,respectively on the day of,and at 1,6 and 12 months after surgery.There were signifi-cant differences in the score among different time points(F=37.167,P＜0.01).LCGM model constructed by quadratic estimation showed that the trajectory of the cardiovascular health score in CHD patients after PCI could be divided into three types:growth type(98 cases,52.69％),sta-ble type(67 cases,36.02％)and volatile type(21 cases,11.29％).Statistical difference was ob-served in the score among the post-PCI CHD patients with different education level,per capita monthly family income,duration of coronary artery disease,and number of comorbid chronic dis-eases(P＜0.05,P＜0.01).The education level,per capita monthly family income,duration of cor-onary heart disease,and number of comorbid chronic diseases were all influencing factors for the trajectory of cardiovascular health score in the CHD patients after PCI(P＜0.01).Conclusion There are three types of trajectory in cardiovascular health score of CHD patients after PCI.Edu-cation level,family per capita monthly income,duration of coronary heart disease and number of chronic diseases are independent factors affecting the score of the patients.

Objective To analyze the trajectory of cardiovascular health score and its influencing factors in patients with CHD after PCI based on LCGM.Methods A total of 186 CHD patients undergoing PCI in our hospital from June 2021 to June 2023 were enrolled,and all of them re-ceived an assessment for cardiovascular health scores.LCGM analysis was performed on the change in their postoperative cardiovascular health scores.Multivariate logistic regression analysis was applied to identify the factors affecting the trajectory of the change in the scores of the patients.Results The cardiovascular health score was 6.34±1.38,6.76±1.44,7.49±1.85,and 8.06±2.07,respectively on the day of,and at 1,6 and 12 months after surgery.There were signifi-cant differences in the score among different time points(F=37.167,P＜0.01).LCGM model constructed by quadratic estimation showed that the trajectory of the cardiovascular health score in CHD patients after PCI could be divided into three types:growth type(98 cases,52.69％),sta-ble type(67 cases,36.02％)and volatile type(21 cases,11.29％).Statistical difference was ob-served in the score among the post-PCI CHD patients with different education level,per capita monthly family income,duration of coronary artery disease,and number of comorbid chronic dis-eases(P＜0.05,P＜0.01).The education level,per capita monthly family income,duration of cor-onary heart disease,and number of comorbid chronic diseases were all influencing factors for the trajectory of cardiovascular health score in the CHD patients after PCI(P＜0.01).Conclusion There are three types of trajectory in cardiovascular health score of CHD patients after PCI.Edu-cation level,family per capita monthly income,duration of coronary heart disease and number of chronic diseases are independent factors affecting the score of the patients.

Objective To study the effects of formaldehyde on the level of microelements in mouse liver and blood and explore the microelements as biomarker in injury induced by formaldehyde.Methods The mice were randomly divided into control,low(1/40LD50),moderate(1/20LD50) and high(1/10LD50) dose groups exposed to formaldehyde by inhalation(n=20),two hours per day for three and six weeks,and than the contents of Cu,Fe,Zn and Mn in mouse liver and blood were measured.Results The contents of Fe,Zn and Mn in liver in high dose group three weeks after exposure and the contents of Fe and Zn in liver in high dose group six weeks after exposure were significantly lower than those in control group(P

0. 05) while UDS was induced by Polygala tenyi folia willd with various doses comparing with normal control. Nevertheless, Polygala tenyi folia willd in doses ranged from 1. 0 to 4. 0 g ? kg-1 inhibited strikingly UDS induced by Pb (CH3COO)2 (P

Objective To evaluate the quality of BAC clones for CGH microarrays in the detection of tumors.(Methods Chromosome) preparations were made from peripheral blood of the healthy volunteers in the conventional manner.The locations of BAC(RPCI-11 library and CTC library) within the region of interest were compiled from information archived by the UCSC and the NCBI.Probes were labeled by nick-translation with biotin-16-dUTP or digoxigenin-11-dUTP.Precise localization of each BAC was confirmed using normal metaphase chromosomes by FISH technique.The copy number and molecular organization of the region of 223 BAC clones which were crucial in the development and progression of human cancers were investigated.Results The FISH analysis indicated the normal BAC clones accounted for 81.62% of the total(186/223);the abnormal clones with additional FISH noises accounted for 13.45%(30/223);those with wrong localization pattern was 3.58%(8/223),and those with no bacterial growth was 1.35%(3/223),respectively.Conclusion FISH technique is effective and useful in the identification of BAC clones for array CGH.

OBJECTIVETo study the effect of Percoll selection technique on normal morphology and acrosin activity of human spermatoza.

METHODSThe sperm morphology and sperm acrosin activity were analyzed by automated sperm morphology analyzer(ASMA) and spectrocolorimetry.

RESULTSThe normal morphology sperm rate and acrosin activity were significantly increased after Percoll selection technique (P < 0.001).

CONCLUSIONPercoll selection technique could affect normal morphology sperm ratio and acrosin activity.

Acrosin ; metabolism ; Adult ; Centrifugation, Density Gradient ; Fertilization in Vitro ; Humans ; Male ; Spermatozoa ; cytology ; enzymology

Objective:To construct the recombinant nonstructural P_2C plasmid in coxsackie B4 virus.Methods:Using the RT-PCR,we fishing the nonstructural protein P_2C cDNA fraction from Coxsackie B4 virus(CVB4) and cloning into the PUCm-T vector, then transfer it into E.coli to construct the recombinant plasmid.Results:Using the total RNA of Coxsackie B4 virus as model, we amplied 987 bp fraction and cloned into PUCm-T vector identifying with BamH Ⅰ and Hind Ⅲ double enzyme.Its products were consistent with nonstructural protein P_2C PCR productions.Conclusion:The recombinant nonstructural P_2C plasmid was constructed succesfully in coxsackie B4 virus.