This document targets digital human metabolic chamber platforms and specifies construction standards and testing protocols covering the full lifecycle of " build-test-operate." It encompasses chamber engineering and environmental control, digital platform and cybersecurity architecture, metabolic measurement and multimodal data acquisition, as well as quantitative system performance and data quality indicators with verifiable acceptance tests. By standardizing architecture, interfaces, and quality control, the specification enables multicenter data interoperability and harmonized quality management, providing high-quality, verifiable, and traceable infrastructure to support precision metabolism research and clinical translation in China.

Objective:To investigate gut microbiota differences between individuals with and without colorectal adenoma(CRA)and to identify gut microbes associated with CRA.Methods:This cross-sectional study analyzed the gut microbiota of 100 patients with CRA and 68 individuals without CRA(aged 40-75 years)who underwent colonoscopies between March 2021 and March 2022 at Tianjin Nankai Hospital.Fecal samples were sequenced for the V3-V4 region of the bacterial 16S rRNA gene using the Illumina NovaSeq platform.Results:Compared to the non-CRA group,the CRA group exhibited reduced relative abundances of identified and unidentified Lachnospiraceae,with increased Faecalibacterium and Streptococcus.In the non-CRA group,the relative abundances of Coprococcus,unidentified Clostridiaceae,and Clostridium were higher.LEfSe analysis revealed significant enrichment of Gammaproteobacteria,Proteobacteria,Enterobacteriales,and Faecalibacterium in the CRA group,while the non-CRA group was enriched for Moraxellaceae,Acinetobacter,and Anaerostipes.Conclusions:These findings suggest a discernible disparity in the gut microbiota structure between CRA patients and individuals without adenoma.The enrichment of potential pathogenic taxa,such as Faecalibacterium and Streptococcus,in the CRA group suggests a possible association with adenoma development.

Objective To explore the mediating role of mindful attention and awareness in depressive symptoms and insomnia severity among patients with comorbid depression and insomnia.Methods A cross-sectional study was conducted,enrolling 267 patients with comorbid depression and insomnia who were treated in the outpatient Department of Medical Psychology of Second Affiliated Hospital of Army Medical University,from March to May 2024.Basic demographic and clinical data were collected using a general information questionnaire.Depressive symptom severity was measured via the Patient Health Questionnaire-9(PHQ-9),insomnia severity via the Insomnia Severity Index(ISI),perceived stress via the Perceived Stress Scale-10(PSS-10),and mindful attention and awareness via the Mindful Attention Awareness Scale(MAAS).Pearson correlation analysis was used to examine the correlations between depressive severity,insomnia severity,perceived stress,and mindful attention and awareness.Mediation analysis was performed using Process 4.1.Results The PHQ-9 score was(13.80±5.98)and the ISI score was(17.10±5.56)in the 267 patients.Pearson correlation analysis showed that depressive severity and insomnia severity were positively correlated with perceived stress(r=0.531,0.351,P<0.001)and negatively correlated with mindful attention and awareness(r=-0.373,-0.350,P<0.001).Mediation analysis using Process 4.1 indicated that the combined mediating effect of mindful attention and awareness and insomnia between perceived stress level and depressive level was 0.157,with a 95%confidence interval(CI)of 0.102～0.217,and the total mediating effect was significant(P<0.001).Conclusion Perceived stress directly positively affects depression and indirectly exacerbates depression through insomnia as a mediator,and mindful attention and awareness can weaken the promoting effect of perceived stress on insomnia.

Objective:To investigate gut microbiota differences between individuals with and without colorectal adenoma(CRA)and to identify gut microbes associated with CRA.Methods:This cross-sectional study analyzed the gut microbiota of 100 patients with CRA and 68 individuals without CRA(aged 40-75 years)who underwent colonoscopies between March 2021 and March 2022 at Tianjin Nankai Hospital.Fecal samples were sequenced for the V3-V4 region of the bacterial 16S rRNA gene using the Illumina NovaSeq platform.Results:Compared to the non-CRA group,the CRA group exhibited reduced relative abundances of identified and unidentified Lachnospiraceae,with increased Faecalibacterium and Streptococcus.In the non-CRA group,the relative abundances of Coprococcus,unidentified Clostridiaceae,and Clostridium were higher.LEfSe analysis revealed significant enrichment of Gammaproteobacteria,Proteobacteria,Enterobacteriales,and Faecalibacterium in the CRA group,while the non-CRA group was enriched for Moraxellaceae,Acinetobacter,and Anaerostipes.Conclusions:These findings suggest a discernible disparity in the gut microbiota structure between CRA patients and individuals without adenoma.The enrichment of potential pathogenic taxa,such as Faecalibacterium and Streptococcus,in the CRA group suggests a possible association with adenoma development.

This document targets digital human metabolic chamber platforms and specifies construction standards and testing protocols covering the full lifecycle of " build-test-operate." It encompasses chamber engineering and environmental control, digital platform and cybersecurity architecture, metabolic measurement and multimodal data acquisition, as well as quantitative system performance and data quality indicators with verifiable acceptance tests. By standardizing architecture, interfaces, and quality control, the specification enables multicenter data interoperability and harmonized quality management, providing high-quality, verifiable, and traceable infrastructure to support precision metabolism research and clinical translation in China.

Objective:To evaluate the clinical effect of YM-W type gastrointestinal motility treatment device on the quality of bowel preparation of elderly patients with constipation. Methods:A convenient sampling method was used to select 70 elderly patients with constipation who underwent colonoscopy examination at the Sixth People's Hospital affiliated to Shanghai Jiao Tong University School of Medicine from July 2022 to May 2023. The patients were randomly divided into a control group and an intervention group by using random number method,with 35 cases in each group. The control group received routine intestinal preparation,while the intervention group underwent a gastrointestinal motility treatment with 30 minutes on the basis of control group. The scores of Boston bowel preparation scale,first defecating time,usage time from defecating to no-slag,and the amount of laxatives used from defecating to no-slag of two groups were compared after intervention. Results:For the scores of Boston bowel preparation scale,the left colon (2.9±0.55),transverse colon (2.2±0.69),right colon (2.2±0.58) and total score (7.69±1.69) in the intervention group were all higher than those (2.6±0.60,2.6±0.65,1.8±0.76,6.66±1.83) in the control group,with statistically significant differences between the two groups (Z=-0.304,-2.84,-2.488,-3.141,P<0.05),respectively. Both of the first defecating time and usage time from defecating to no-slag in the intervention group were significantly shorter than those in the intervention group (Z=-3.64,-2.52,P<0.05). The amount of laxatives used from defecating to no-slag in the intervention group was less than that in the control group (Z=-1.18,P<0.05). The adverse reaction rate of patients in the intervention group was significantly lower than that in the control group (F=31.8,P<0.05),and the difference between the two groups was statistically significant. Conclusion:The gastrointestinal motility treatment device can enhance the cleanliness quality of bowel before elderly patients with constipation undergo colonoscopy examination,which is worth to be applied in clinical practice.

Objective:To evaluate the clinical effect of YM-W type gastrointestinal motility treatment device on the quality of bowel preparation of elderly patients with constipation. Methods:A convenient sampling method was used to select 70 elderly patients with constipation who underwent colonoscopy examination at the Sixth People's Hospital affiliated to Shanghai Jiao Tong University School of Medicine from July 2022 to May 2023. The patients were randomly divided into a control group and an intervention group by using random number method,with 35 cases in each group. The control group received routine intestinal preparation,while the intervention group underwent a gastrointestinal motility treatment with 30 minutes on the basis of control group. The scores of Boston bowel preparation scale,first defecating time,usage time from defecating to no-slag,and the amount of laxatives used from defecating to no-slag of two groups were compared after intervention. Results:For the scores of Boston bowel preparation scale,the left colon (2.9±0.55),transverse colon (2.2±0.69),right colon (2.2±0.58) and total score (7.69±1.69) in the intervention group were all higher than those (2.6±0.60,2.6±0.65,1.8±0.76,6.66±1.83) in the control group,with statistically significant differences between the two groups (Z=-0.304,-2.84,-2.488,-3.141,P<0.05),respectively. Both of the first defecating time and usage time from defecating to no-slag in the intervention group were significantly shorter than those in the intervention group (Z=-3.64,-2.52,P<0.05). The amount of laxatives used from defecating to no-slag in the intervention group was less than that in the control group (Z=-1.18,P<0.05). The adverse reaction rate of patients in the intervention group was significantly lower than that in the control group (F=31.8,P<0.05),and the difference between the two groups was statistically significant. Conclusion:The gastrointestinal motility treatment device can enhance the cleanliness quality of bowel before elderly patients with constipation undergo colonoscopy examination,which is worth to be applied in clinical practice.

Humans ; COVID-19 ; Quarantine ; Renal Dialysis ; SARS-CoV-2 ; Hospitals

Objective:To explore the clinicopathologic features and renal prognosis of patients with post-transplant membranous nephropathy (MN).Methods:Patients with allograft biopsy-proven MN were reviewed retrospectively and divided into unknown etiology group (A, n=12) and recurrent membranous nephropathy (rMN) group (B, n=7). Their clinicopathological data and renal prognosis were assessed and compared.Results:No differences existed in the proportion of living-related donor or post-transplant allograft function. Group B had recurrence at 16.4 months after transplantation and it was significantly shorter than group A. Allograft impairment manifested as proteinuria, nephrotic syndrome and/or renal insufficiency in both groups. The positive rate of serum anti-PLA2R antibody and renal PLA2R staining was significantly higher in group B than that in group A. Similarly, the intensity of IgG4 subtype staining was also stronger in group B than that in group A. The 5-year cumulative renal survival rates from end-stage renal disease (ESRD) were 77.8% and 66.7% in groups A and B respectively. No significant inter-group difference existed in renal prognosis.Conclusions:Anti-PLA2R antibody plays an important role in the recurrence of rMN after renal allografting. PLA2R staining is useful for detecting primary disease and its sensitivity is higher than that of serum anti-PLA2R antibody. Rituximab is an effective treatment for post-transplant MN. Follow-up studies with a larger sample size are required for further verification.

Objective:To summarize the patient profiles and therapeutic efficacies of ABO-incompatible living-related kidney transplantations at 19 domestic transplant centers and provide rationales for clinical application of ABOi-KT.Methods:Clinical cases of ABO-incompatible/compatible kidney transplantation (ABOi-KT/ABOc-KT) from December 2006 to December 2009 were collected. Then, statistical analyses were conducted from the aspects of tissue matching, perioperative managements, complications and survival rates of renal allograft or recipients.Results:Clinical data of 342 ABOi-KT and 779 ABOc-KT indicated that (1) no inter-group differences existed in age, body mass index (BMI), donor-recipient relationship or waiting time of pre-operative dialysis; (2) ABO blood type: blood type O recipients had the longest waiting list and transplantations from blood type A to blood type O accounted for the largest proportion; (3) HLA matching: no statistical significance existed in mismatch rate or positive rate of PRA I/II between two types of surgery; (4) CD20 should be properly used on the basis of different phrases; (5) hemorrhage was a common complication during an early postoperative period and microthrombosis appeared later; (6) no difference existed in postoperative incidence of complications or survival rate of renal allograft and recipients at 1/3/5/10 years between ABOi-KT and ABOc-KT. The acute rejection rate and serum creatinine levels of ABOi-KT recipients were comparable to those of ABOc-KT recipients within 1 year.Conclusions:ABOi-KT is both safe and effective so that it may be applied at all transplant centers as needed.