Loss of protein homeostasis is a hallmark of cellular senescence, and ribosome pausing plays a crucial role in the collapse of proteostasis. However, our understanding of ribosome pausing in senescent cells remains limited. In this study, we utilized ribosome profiling and G-quadruplex RNA immunoprecipitation sequencing techniques to explore the impact of RNA G-quadruplex (rG4) on the translation efficiency in senescent cells. Our results revealed a reduction in the translation efficiency of rG4-rich genes in senescent cells and demonstrated that rG4 structures within coding sequence can impede translation both in vivo and in vitro. Moreover, we observed a significant increase in the abundance of rG4 structures in senescent cells, and the stabilization of the rG4 structures further exacerbated cellular senescence. Mechanistically, the RNA helicase DHX9 functions as a key regulator of rG4 abundance, and its reduced expression in senescent cells contributing to increased ribosome pausing. Additionally, we also observed an increased abundance of rG4, an imbalance in protein homeostasis, and reduced DHX9 expression in aged mice. In summary, our findings reveal a novel biological role for rG4 and DHX9 in the regulation of translation and proteostasis, which may have implications for delaying cellular senescence and the aging process.
G-Quadruplexes ; Cellular Senescence ; Ribosomes/genetics* ; Humans ; Animals ; Mice ; DEAD-box RNA Helicases/genetics* ; Protein Biosynthesis ; RNA/chemistry* ; Neoplasm Proteins

At present, the teaching objects of clinical pharmacists have gradually changed from merely university students to complex and diverse groups including clinical pharmacist interns and others. However, the traditional teaching model cannot be tailored to different groups. In addition, it increases the burden of clinical pharmacists while not ensuring teaching quality. This study aims to classify teaching objects according to their characteristics, so as to provide individualized knowledge and service. Compared with the traditional way of teaching, this new method may make teaching more relevant and reduces the teaching load of teachers, which is of great significance to the training of clinical pharmacy personnel.

DNA methylation is an epigenetic modification form with the most extensive and in-depth studying. DNA methylation is a powerful tool for tumor classification in the central nervous system (CNS), which is used in the molecular classification of various tumors, such as ependyma and meningioma, to accurately predict tumor biological behavior and survival prognosis, and to provide important information for individualized treatment. DNA methylation will also become an important auxiliary diagnostic method for the identification of different types of CNS tumors, improve the accuracy of pathological diagnosis, and even help to find new tumor entities. In the current review, we focus on the role of DNA methylation in the molecular classification and pathological diagnosis of CNS tumors, in order to provide more enlightenment for accurate diagnoses and treatments of tumors.

Objective To explore the effects of feedback health education model in breathing exercises among gastric cancer patients after surgery.Methods From June 2015 to June 2016, we selected 98 gastric cancer patients with radical resection of Fudan University Shanghai Cancer Center as subjects by purposive sampling, all of the patients were divided into two groups with the random number table, 49 cases in each group. Patients of control group accepted routine health education model. Patients of observation group were treated with feedback health education model. We compared the compliance of breathing exercises, lung function and 6-minute walking distance of patients in two groups.Results The compliance of breathing exercises and lung function of patients of observation group were better than those of control group 12 months after surgery; the 6-minute walking distance of patients of observation group were longer than those of control group with significant differences (P<0.01).Conclusions The application of feedback health education model in breathing exercises among gastric cancer patients after surgery can improve the compliance of breathing exercises, mid-term lung function and movement ability of gastric cancer patients. It is worth to be used widely in clinical application.

Objective:To investigate the effects of moxibustion on the serum metabolism in healthy human body based on the 1H nuclear magnetic resonance (1H NMR) metabolomics technology, and to find the differences in metabolites, as well as to elucidate the effects of moxibustion on healthy human body from the viewpoint of global metabolism. Methods:Sixty subjects of healthy young men from the enrolled students were randomly divided into a moxibustion group and a control group using random number table, with 30 cases in each group. Subjects in the moxibustion group accepted mild moxibustion on the right Zusanli (ST 36), once a day, 15 min for each time, and continuous treatment for 10 d; those in the control group did not receive any intervention. There were 28 cases in the moxibustion group and 23 cases in the control group after interventions. On the 1st day, 5th day and 10th day of the intervention, serum samples were collected from subjects of the two groups, and metabolic spectra were obtained by the1H NMR technology. Results: Before and after the intervention, serum1H NMR of the moxibustion group was significantly different, while the difference was insignificant in the control group. Metabolite changes in the moxibustion group were mainly in low density lipoprotein (LDL)/very low density lipoprotein (VLDL), valine, isoleucine, leucine, lactic acid, glutamine, citric acid, polyunsaturated fatty acids, creatine, glycine, glycerol, glucose, tyrosine, histidine, formic acid, alanine, lysine, acetic acid, and glutamic acid. Conclusion:Moxibustion can cause changes of serum metabolic patterns in healthy human by influencing the concentrations of branched-chain amino acids, polyunsaturated fatty acids, and other metabolites to strengthen body's metabolisms of amino acids and fatty acid.

Objective: To establish a rabbit unstable bladder and Partial Bladder Outlet Obstruction (BOO) model, and to study on urodynamic changes. Methods: 30 male New Zealand rabbits were divided into control group and operative group. After 8 weeks, urodynamic changes were determined after they were anaesthetized by ketamine and droperidol. Results: Prominent changes of Main urodynamic parameters were found between the operative group and control group. The incidence rate of unstable bladder was 60%. Conclusion: The method of establishing rabbit model of Partial BOO is successful. It provides a platform for the study on the changes of pathology and pathophysiology of human chronic partial BOO and treatment of this kind of diseases.

OBJECTIVE:To understand the characteristics of adverse drug reactions(ADR)for their rational use in clin-ic.METHODS:242ADR cases had been collected from July1,2002to June30,2003in our hospital.All the cases were analyzed in respect of drug species,categories of anti-infective drugs,involved organ systems,ways of administration and ages of the patients.RESULTS:Among the242cases of ADR,the reasons were found to be related to use of anti-infective drugs,the quality of injection fluid of traditional Chinese medicine,ways of intravenous administration,the older age of the patients,ADR were mainly manifested as skin and local lesion.CONCLUSION:Rational use of anti-infective drugs and the good quality of the traditional Chinese medicine injections should be emphasized to decrease the occurrence of ADR.