Objective:To explore the expression of chemokine ligand 1(CCL1)in oral squamous cell carcinoma(OSCC)and its effect on the proliferation,migration and invasion of human oral tongue squamous cell carcinoma cells(HSC-4).Methods:28 OSCC tissue samples and clinical characteristic data of patients were collected at the Affiliated Stomatological Hospital of Southwest Medical University between January 2018 and June 2020,as well as 10 normal gingival tissue samples removed during the extraction of impacted teeth.OSCC cells HSC-4 were cultured routinely and divided into the control group(without virus),the NC group(transfected with control lentiviral vector),the shCCL1 group(transfected with knockdown CCL1 lentiviral vector),and the CCL1 group(culture medium containing 60 ng/mL CCL1 recombinant protein).Immunohistochemistry and WB were used to detect the expression of CCL1 in OSCC tissues and cells,and analyze the correlation between its expression level and the clinical features of patients.qPCR,CCK-8 assay,plate cloning assay,cell scratch test,Transwell assay and flow cytometry were used to detect the expression of CCL1 mRNA,the proliferation,migration and invasion abilities and the apoptosis of HSC-4 cells respectively.Results:CCL1 protein was highly expressed in OSCC tissues and HSC-4 cells(all P<0.01)and its expression was related to the clinical stage of tumors(P<0.05).The expression of CCL1 in HSC-4 cells was successfully knocked down(P<0.000 5).Knocking down the expression of CCL1 could inhibit the proliferation(P<0.05 or P<0.01),migration and invasion(all P<0.05)of HSC-4 cells,and promote its apoptosis(all P<0.05).CCL1 recombinant protein treatment resulted in the opposite effects(P<0.05,P<0.01,P<0.000 1).Conclusion:CCL1 is highly expressed in OSCC and its expression is correlated with the clinical stage of OSCC.CCL1 may take part in regulating the proliferation,migration,invasion and apoptosis of HSC-4 cells.

After nearly 40 years of development,MRI has become an important imaging diagnostic method in clinical practice.Non-contrast enhanced MRI combined with contrast-enhanced MRI can provide multidimensional information,significantly improving lesion detectability and supporting the entire disease management cycle.With the decentralization of medical resources,contrast-enhanced MR technologies(such as magnetic resonance angiography,dynamic contrast-enhanced MRI and dynamic susceptibility contrast-enhanced MRI,etc.)have become popular in primary healthcare facilities.As a critical adjunct to contrast-enhanced imaging,high-pressure injection technology for contrast agents plays a determinant role in ensuring imaging quality and diagnostic validity.This technology offers distinct advantages in precise control of injection parameters(flow rate,dosage,timing),maintenance of bolus integrity and enhancement of inter-study reproducibility.However,the absence of standardized international and domestic guidelines for its application currently hinders the harmonization of imaging data and limits cross-institutional interoperability of diagnostic results.To address this gap,an expert group has developed the"Expert Consensus on the Application of High-Pressure Injection Technology for Magnetic Resonance Contrast Agents",referencing domestic and international guidelines and literature,and in conjunction with the current application status in the country.This consensus aims to provide radiology professionals with safe and effective technical application guidance and to promote standardized clinical practice.

The phenomenon of adolescent suicide has become a serious challenge to global public health, and suicidal behavior can directly threatening the lives of adolescents.Attempted suicide during adolescence has long-term negative impacts on their health in adulthood.With the continuous development of artificial intelligence technology, machine learning has demonstrated markedly superior performance compared to traditional assessment tools in predicting the risk of suicide among adolescents.Therefore, this article reviews the application and significance of machine learning in predicting suicidal behavior among adolescents.It mainly focuses on machine learning-related concepts, the utilization of multimodal data such as text and voice, as well as the in-depth analysis of algorithmic performance.However, this learning technology continues to encounter challenges pertaining to data quality, overfitting, model interpretability as well as ethical considerations.In future practical applications, various factors, including data characteristics, problem requirements, time costs and algorithm performance, should be comprehensively considered to develop a more accurate predictive model for adolescent suicidal behavior, thereby safeguarding the mental health of adolescents.

At present, liver transplantation is the most effective method for the treatment of various end-stage liver diseases, and electronic health technology is a new medical service model, which can be used as an efficient and cost-effective alternative to the health management of liver transplant recipients. This paper summarizes the application of electronic health technology in postoperative health management of liver transplant recipients, including applications, robots, video conferencing, tablet computers, text messages, telephones and wearable devices, etc., summarizes the implementation effect of electronic health technology in health management of liver transplant recipients, and analyzes the limitations of research and application at the present stage. In order to provide basis and reference for Chinese medical staff to carry out electronic health technology in the health management of liver transplant recipients.

The symptom clusters increase the symptom distress of liver transplant recipients, reduces their treatment and nursing compliance, and greatly affects the prognosis quality of life of liver transplant recipients. This article reviews the concept, research status, assessment tools, influencing factors, and interventions for symptom clusters in liver transplant recipients, aiming to help clinical nurses formulate targeted interventions based on symptom clusters, provide effective symptom management for liver transplant recipients, reduce the physical and psychological damage caused by symptom clusters, and continuously improve the quality of life of liver transplant recipients.

OBJECTIVES: This study aimed to investigate the perception of dental outpatient care quality from multiple perspectives of administrators, physicians, nurses, and patients and propose nursing care quality evaluation indices that are consistent with the clinical reality to provide reference for the construction of a scientific, systematic, and comprehensive dental outpatient care quality evaluation system. METHODS: A total of 39 interviewees, including 7 administrators, 11 doctors, 11 nurses, and 10 patients, were selected for semi-structured in-depth interviews in five regionally representative tertiary-level A stomatological specialty hospitals nationwide during January-April 2024 by using a multistage sampling method. Colaizzi 7-step analysis was used to analyze and summarize the interview data. Themes were extracted on the basis of the Structure-Process-Outcome (SPO) three-dimensional quality assessment model. RESULTS: Five main themes and 15 secondary themes were extracted from three quality dimensions: structure, process, and result. The related topics of structural quality were as follows: disinfection and isolation norms, equipment and consumable management, nursing manpower ratio and nurse education structure, and emergency capability. The related topics of process quality were as follows: pre-diagnosis risk assessment, patient triage and guidance, communication and attitude, health education, humanistic care, continuous care, specialty operation, and four-hand operation. The related topics of result quality were as follows: satisfaction, adverse event management and analysis, effective complaints and disputes. CONCLUSIONS Structure quality is the foundation, process quality is the core, and result quality is the key in the evaluation of the quality of oral outpatient care. The standardization of disinfection and isolation, equipment and consumable management, allocation of reasonable nursing manpower and post capacity, implementation of high-quality nursing services, and improvement of the quality and satisfaction of medical cooperation are necessary guarantees to ensure the quality of oral outpatient care.
Humans ; Quality of Health Care ; Ambulatory Care/standards* ; Dental Care/standards* ; Outpatients

OBJECTIVES: This study aimed to construct an evaluation index system for nursing quality management in outpatient dental clinics based on the structure-process-outcome model and provide an objective standard for the evaluation of nursing quality in outpatient dental clinics. METHODS: Through literature review, multi-subject interviews, and expert meetings, the first draft of the evaluation index for nursing quality management in outpatient dental clinics was formulated. The Delphi method was adopted to select and invite 15 experts in the fields of hospital infection management, nursing management, and specialized oral care from across the country to modify the first draft. RESULTS: The positive coefficients of the experts in the two rounds of consultation were 86.7% and 92.3%, respectively. The total authority coefficients of the experts were 0.791 and 0.717, respectively. The mean scores of the importance and feasibility of the third-level indices in the two rounds of consultation were all ≥4.333; the coefficients of variation were all ≤0.150; and the Kendall's coordination coefficients were 0.308 and 0.184 respectively, with P<0.05 for all. These results indicated that the experts were motivated to participate in this study. They recognized the importance and feasibility of the overall items in this index system, and their opinions were relatively consistent. Finally, an evaluation index system, which included 3 first-level indices, 7 second-level indices, 22 third-level indices, and 69 index connotations, for nursing quality management in outpatient dental clinics was determined. The weights of the three first-level indicators were all 0.333. Patient satisfaction (0.076, outcome dimension), hand hygiene (0.061, outcome dimension), chair care ratio (0.057, structural dimension), and turnover rate (0.057, structural dimension) were the top tertiary indicators in terms of portfolio weight. CONCLUSIONS The construction method of the evaluation index system for nursing quality management in outpatient dental clinics is scientific and reliable. It can provide a reference for the evaluation of the management level of nursing quality in outpatient dental clinics and promote the continuous improvement of nursing quality in outpatient dental clinics.
Humans ; Dental Clinics ; Delphi Technique

OBJECTIVE To investigate the result of human immunodeficiency virus(HIV)screening for the people visiting to a three-A hospital of Sichuan Province and analyze the prevalence of complications with hepatitis B virus(HBV)infection,hepatitis C virus(HCV)infection and Treponema pallidum(TP)infection in the emerging HIV infection patients.METHODS The result of HIV screening for the people who visited to Ziyang Central Hos-pital from Jan.1,2020 to Dec.31,2024 and the test results of HBV,HCV and TP for the emerging HIV infec-tion patients were collected and were summarized and statistically analyzed by SPSS.0 software.RESULTS Totally 289 891 case-times were tested for HIV,1529 cases were previously diagnosed with HIV,465 of whom were tested posi-tive for the first time,there was significant difference in the positive rate of test for the first time among the 5 years(x2=15.998,P=0.003).Totally 353 cases were confirmed positive among the 465 primary positive screening cases.Among the emerging HIV infection patients,the positive rate was higher in the male than in the female(x2=141.141,P＜0.001),and the positive rate was high among the population aged more than 40 year old(x2=11.448,P＜0.001),mi-grant workers(x2=270.110,P＜0.001)and low education level population(x2=25.911,P＜0.001).The detection rate of gp41 was up to 100.00％in strip type testing.The analysis of the ratio of relative light unit(RLU)to Cutoff val-ue(COI)in the initial screening experiment showed that when COI was greater than 50,all of the confirmed tests were positive,when COI ranged between 1 and 5,the false positive rate was 97.06％.The incidence of complica-tion with HBV infection in the emerging HIV infection patients was increased year by year(x2=20.355,P＜0.001),and the incidence of complication with HCV infection was increased in recent two years(x2=10.690,P=0.030).CONCLUSIONS There is no obvious rise of positive rate of HIV screening among the people visiting to the hospital in recent 5 years.The sensitivity of the primary screening of clinical laboratory is high without posi-tive missing test.The positive rates of HBV and HCV are increased among the emerging HIV infection patients.

Objective To explore the expression of macrophage colony-stimulating factor(MCSF)in oral squamous cell carcinoma(OSCC)and its effects on the proliferation,apoptosis,and migration of OSCC cells.Methods Normal gingival and OSCC tissues were collected,and MCSF protein expression was detected using immunohistochemistry and Western blotting.HSC-4 cells were divided into control(no transfection),shNC(transfection with sequence-free plasmid vector lentivirus),and shMCSF(transfection with silent MCSF plasmid vector lentivirus)groups.The expression of MCSF mRNA and protein in HSC-4 cells was detected using quantitative real-time PCR and Western blotting,respectively.Scratch and Transwell assays were used to detect the migration ability of HSC-4 cells.The TUNEL assay determined the apoptosis ability of HSC-4 cells,while a colony formation assay detected the proliferation ability of HSC-4 cells.Results MCSF was highly expressed in OSCC tissues and HSC-4 cells but weakly expressed in normal gingival tissues and Hacat cells.The migration and proliferation ability of HSC-4 cells in the shMCSF group was lower than that in the shNC group(P＜0.05).The apoptosis ability of HSC-4 cells in the shMCSF group was higher than that in the shNC group(P＜0.05).Conclusion MCSF is upregu-lated in OSCC tissues,promoting cell migration and proliferation,while also reducing the apoptosis of OSCC cells.

This article focused on promoting the high-quality development of oral specialty nursing through new-quality productive forces. New-quality productive forces injected impetus into the innovation and development of the medical and health system. At the time, the development of oral specialty nursing faced some challenges. In terms of talent cultivation, there was a lack of undergraduate education in institutional education, and on-the-job education needed to be further deepened. It was necessary to strengthen undergraduate degree education, innovate training models, and unify the qualification certification. In the aspect of technological innovation, new technologies such as digitalization had changed oral diagnosis and treatment. Nursing staff were required to enhance their capabilities in technological application and scientific research. In the field of service upgrading, oral nursing services needed to satisfy the demands of the aging society and residents through deepening the connotation and expanding the denotation, which involved implementing refined nursing, multidisciplinary collaboration, and "Internet + Nursing Services". In conclusion, multi-party collaboration could promote the high-quality development of oral specialty nursing and contributed to the construction of healthy China.