Objective To investigate the effects of patient bone mass differences on the stability of unicondylar knee arthroplasty(UKA)prostheses.Methods A UKA finite element model was established to quantify the effects of five different bone quality conditions on the proximal tibial von Mises stress,bone-prosthesis fixation interface contact stress,and bone-prosthesis fixation interface micromotion,using the medial knee force and joint motion predicted by the individualized UKA musculoskeletal multibody dynamics model as boundary conditions.Results The influences of bone strength on the proximal tibia von Mises stress and bone-prosthesis fixation interface contact stress were not obvious,and the difference in peak values of the proximal tibia von Mises stress between two groups of models with the largest difference in bone strength was not more than 5％,and the difference in peak values of the bone-prosthesis fixation interface contact stress was only 2.37 MPa.However,the influence of bone strength on the bone-prosthesis fixation interface micromotion was significant,and the weaker bones were more prone to cause the bone-prosthesis fixation interface micromotion.However,bone strength had a significant effect on the bone-prosthesis fixation interface micromotion,and weak bone was more likely to cause changes in the bone-prosthesis fixation interface micromotion.Compared to patients with the neutral bone quality,the prosthesis fixation interface micromotion increased by 84.67％at 20％gait cycles for patients with the weakest bone quality.Conclusions UKA patients with a weaker bone quality have a higher risk of prosthesis loosening.It is recommended that surgeons should carefully choose their surgical strategy in order to reduce the rate of postoperative revision in UKA.

Objective To investigate the effects of patient bone mass differences on the stability of unicondylar knee arthroplasty(UKA)prostheses.Methods A UKA finite element model was established to quantify the effects of five different bone quality conditions on the proximal tibial von Mises stress,bone-prosthesis fixation interface contact stress,and bone-prosthesis fixation interface micromotion,using the medial knee force and joint motion predicted by the individualized UKA musculoskeletal multibody dynamics model as boundary conditions.Results The influences of bone strength on the proximal tibia von Mises stress and bone-prosthesis fixation interface contact stress were not obvious,and the difference in peak values of the proximal tibia von Mises stress between two groups of models with the largest difference in bone strength was not more than 5％,and the difference in peak values of the bone-prosthesis fixation interface contact stress was only 2.37 MPa.However,the influence of bone strength on the bone-prosthesis fixation interface micromotion was significant,and the weaker bones were more prone to cause the bone-prosthesis fixation interface micromotion.However,bone strength had a significant effect on the bone-prosthesis fixation interface micromotion,and weak bone was more likely to cause changes in the bone-prosthesis fixation interface micromotion.Compared to patients with the neutral bone quality,the prosthesis fixation interface micromotion increased by 84.67％at 20％gait cycles for patients with the weakest bone quality.Conclusions UKA patients with a weaker bone quality have a higher risk of prosthesis loosening.It is recommended that surgeons should carefully choose their surgical strategy in order to reduce the rate of postoperative revision in UKA.

Objective: To explore mental health status of vocational college students and its association with physical exercise and exercise motivation, so as to provide data support for the promotion of physical exercise, exercise motivation theory and the protection of mental health among vocational college students. Methods: From June 2 to July 2, 2023, a convenience sampling method was used to select 1 763 college students from three vocational colleges in Sichuan, Chongqing, and Guizhou for the survey. Physical Activity Rating Scale (PARS-3), Motives for Physical Activities Measure-Revised (MPAM-R), Patient Health Questionnaire-9 (PHQ-9) and Flourishing Scale (FS) were used to assess physical exercise, exercise motivation, depressive symptoms and flourishing levels of vocational college students. Multiple linear regression analysis was conducted to examine the relationship between physical exercise, exercise motivation, depression as the negative indicator of mental health and flourishing as the positive indicator of mental health. Results: There were negative correlations between physical exercise, exercise motivation and their five factors with depression scores among vocational college students ( r=-0.162--0.133, P <0.01). Physical exercise, health motivation and pleasure motivation were positively correlated with flourishing and its eight factors among vocational college students ( r= 0.054 -0.099, P <0.05). Depression scores and flourishing scores varied significantly across students with different levels of physical exercise and exercise motivation ( F=11.18-15.69, 2.80-16.05, P <0.05). Multiple linear regression analysis indicated that physical exercise, health motivation, and total exercise motivation scores were negatively correlated with depressive symptoms ( β = -0.02 , -0.11, -0.35), and physical exercise, health motivation, pleasure motivation, and total exercise motivation scores were positively correlated with flourishing ( β =0.31, 0.32, 0.50, 0.30) ( P <0.05). Conclusions Physical exercise and exercise motivation are significantly associated with mental health of vocational college students. Enhancing physical exercise and increasing exercise motivation in aspects of health and pleasure can promote mental health of vocational college students.

Objective:To investigate the characteristics of nasal flora and the pathogenic role of differential microbiome in patients with allergic rhinitis (AR) and non-allergic rhinitis (nAR).Methods:Thirty-five patients with AR who attended the rhinology outpatient clinic of the Second Hospital of Harbin Medical University from February to July 2022 were selected. A total of 35 nAR patients were selected as the test group, and 20 cases of healthy people with physical examination at the same period were selected as the control group, including 39 males and 51 females, aged 8 to 55 years. 16SrDNA High-throughput sequencing was used to analyze the relative abundance from nasal flora in the three groups of subjects. Alpha diversity index analysis was conducted with R software, and differences between groups were analyzed with LEfSe, Metastats, and t tests. At the same time, the role of microbiome and its relationship with environmental factors were analyzed with R software. Results:There was a significant difference in the bacterial composition of the samples from the three groups, with the relative abundance of Staphylococcus aureus ( P=0.032) and Corynebacterium proinquum ( P=0.032) within the AR group being significantly higher than that of the nAR group, and that of Lactobacillus murinus, Lactobacillus kunkeei, and Alcaligenes faecalis ( P value was 0.016, 0.005, and 0.001, respectively) being significantly lower than that of the nAR group. The relative abundance of Ackermannia muciniphila within the nAR group was higher than that of the control group ( P=0.009). Correlation analysis of environmental factors showed a negative correlation between Lactobacillus kunkeei and IgE ( P=0.044), and a positive correlation between Lactobacillus murinus and age ( P=0.019). AR and nAR random forest prediction models were constructed for the five genera, respectively, and the area under the curve (AUC) of the models of Streptococcus-SP-FF10, Pseudoalteromonas luteoviolacea, Pseudomonas parafulva, Acinetobacter ursingii, and Azotobacter chroococcum in the AR group was 100% (95%CI: 100% to 100%). The AUC for the Pseudomonas parafulva, Azotobacter chroococcum, Closoridium baratii, Turicibacter-SP-H121, and Streptococcus lutetiensis models in the nAR group was 98.4% (95%CI: 94.9% to 100%). Conclusions:The distribution of nasal flora in AR patients, nAR patients and healthy subjects is significantly different, and the changes of bacterial flora abundance are significantly related to the occurrence of AR and nAR. Combined detection of microbiota has the potential to diagnose AR and nAR patients.

Objective:To analyze the genetic landscape of multiple fusion genes in patients with de novo acute myeloid leukemia (AML) and investigate the characteristics of immunophenotypes and mutations.Methods:The results of multiple fusion genes from 4192 patients with de novo AML were retrospectively analyzed from 2016 to 2020. In addition, the immunophenotypical data and the mutational results from high-through put method were statistically investigated and correlated as well.Results:①Among the 52 targets, 29 different types of fusion genes were detected in 1948 patients (46.47%) with AML, which demonstrated an "exponential distribution" . ② As the age increased, the number of patients with fusion gene increased first and then decreased gradually. The total incidence rate of fusion genes and MLL rearrangment in children were significantly higher than those in adults (69.18% vs 44.76%, 15.35% vs 8.36%) . ③The mutations involving FLT3 and RAS signaling pathway contributed most in patients with MLL rearrangment. ④No specific immunophenotypic characteristics were found in AML patients with MLL or NUP98 rearrangements. Conclusion:Nearly half of AML patients were accompanied by specific fusion gene expression, the proportions of different fusion genes in pediatric and adults patients were different by multiple PCR. The gene mutations and immunophenotype of these AML patients have certain rules.

A 55-year-old female patient with cholecystitis, gallstone and secondary acute pancreatitis received an Ⅳ infusion of cefminox sodium 2. 0 g twice daily. Eight days later,she developed large area of ecchymosis on her skin below the waist and abdomen,especially on that of the lower limbs. Laboratory tests showed the prothrombin time (PT)63. 0 s,prothrombin activity (PTA)0. 12,international normalized ratio (INR)5. 21,coagulation factor Ⅱ activity 0. 32,coagulation factor Ⅶ activity 0. 02,and coagulation factor X activity 0. 27. Vitamin K-dependent coagulation factor deficiency due to cefminox sodium was considered. Cefminox sodium was stopped immediately and an intramuscular injection of mephyton 10 mg once daily was given. Three days later,the laboratory tests showed PT 11. 0 s,PTA 1. 12, INR 0. 94,coagulation factor Ⅱ activity 0. 83,coagulation factor Ⅶ activity 0. 73,and coagulation factor X activity 0. 84.

In the field of bilingualism research, a key scientific question is how bilinguals process two language systems, particularly the effective switch from one language to another, namely bilingual code switching. With the rapid development of neuroimaging techniques, important progresses have been made in bilingual processing studies, especially in code switching. However, consensus has not been achieved regarding the mechanisms of bilingual code switching. Bilingual switching studies using neuropsychological and neuroimaging techniques have gained insights into the temporal and spatial features of the language switching process and the neurological mechanism, which provide direct evidence for the generation mechanism of bilingual code switching.

A 55-year-old female patient with cholecystitis, gallstone and secondary acute pancreatitis received an Ⅳ infusion of cefminox sodium 2. 0 g twice daily. Eight days later,she developed large area of ecchymosis on her skin below the waist and abdomen,especially on that of the lower limbs. Laboratory tests showed the prothrombin time (PT)63. 0 s,prothrombin activity (PTA)0. 12,international normalized ratio (INR)5. 21,coagulation factor Ⅱ activity 0. 32,coagulation factor Ⅶ activity 0. 02,and coagulation factor X activity 0. 27. Vitamin K-dependent coagulation factor deficiency due to cefminox sodium was considered. Cefminox sodium was stopped immediately and an intramuscular injection of mephyton 10 mg once daily was given. Three days later,the laboratory tests showed PT 11. 0 s,PTA 1. 12, INR 0. 94,coagulation factor Ⅱ activity 0. 83,coagulation factor Ⅶ activity 0. 73,and coagulation factor X activity 0. 84.

OBJECTIVE: To improve the quality and efficiency of medication consultation and to promote rational drug use in clinic. METHODS: Totally of 520 cases of medication consultation records from the Affiliated Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of TCM during Oct. 2015-Oct. 2016 were selected according to random number table, and then summarized and analyzed statistically in respects of consultation content, age and type of consultants, department, consulted medicine types, the number of combination of TCM and Western medicine, satisfactory degree of consultants, etc. RESULTS: The main consultation contents included usage and dosage (41. 92％), combination of TCM and Western medicine (12. 88％), etc. Consultants mainly aged 50-70 years old (48. 27％), and were mainly the patients and their family members (95. 96％). Consultants were primarily diagnosed in cardiology department and endocrinology department. Top 3 consulted medicine types were cardiovascular system medicines, nervous system medicines, digestive system medicines, and types of Chinese patent medicines were mainly coordinating medicines and activating blood and removing stasis medicines. Types of TCM combined with Western medicine were less than or equal to 3 kinds in 420 cases (80. 77％). Most patients were very satisfied with outpatient medication consultation (93. 65％). CONCLUSIONS: Rational drug use consultation improves medication compliance of patients, meets the needs of patients on medication safety knowledge, and provides a strong guarantee for safe and rational drug use in clinic.

Objective To explore a new efficient extraction method of dorsal root ganglions(DRGs)of rats by exploring and extracting DRGs reversely along the nerve traveling pathway. Methods The DRGs were extracted by the traditional method of opening the intervertebral foramina and the new method, extracting DRGs reversely along the nerve traveling pathway,respectively. The time consuming and the number of intact DRGs obtained with these two method were compared. Results The number of intact DRGs(L3-5segments,both sides)extracted from each rat with the traditional method was(3.08 ± 1.31),and the average time consuming of each DRG was(5.58 ± 1.21)min. As for the new method ,the number of intact DRGs extracted from each rat was(4.29 ± 1.08), and the average time consuming was(1.69 ± 0.91)min,significantly better than that of the traditional method(P < 0.05 for both). Conclusions The new method of exploring and extracting DRGs reversely along the nerve traveling pathway is more efficient for obtaining intact DRGs of rats,providing more useful tissue materials for subsequent culture and morphological studies of DRG cells.