Hemp seed oil serves as the core pharmacodynamic part of hemp seed， containing various active constituents including fatty acids and their esters （such as linoleic acid， methyl linolenate， etc.）， tocopherols （such as α -tocopherol， β -tocopherol， etc.）， polyphenols （such as caffeic acid， quercetin， etc.）， and cannabinoids （such as cannabidiolic acid， cannabinol， etc.）. Pharmacological studies demonstrate that hemp seed oil exerts anti-oxidation and anti-aging， blood-lipid-lowering and anti-atherosclerotic， neuroprotective， anti-inflammatory effects， as well as functions in regulating intestinal microecology and improving intestinal mucosal barrier function. Its mechanisms are related to scavenging free radicals， activating endogenous antioxidant enzymes， improving the function of central cholinergic system， mitigating neuroinflammatory responses， regulating the balance of inflammatory factors， interfering with inflammatory signaling pathways， and optimizing the structure of intestinal microbiota. In future studies， a correlation network of “chemical component group-multi-target pharmacodynamic effect” can be constructed according to the chemical characteristics of hemp seed oil， and its pharmacodynamic evaluation for chronic diseases can be expanded， so as to promote its in-depth development and clinical transformation.

Objective To explore the efficacy of the"one disease,one product"rehabilitation nursing under the follow-up nursing mode in the postoperative recovery process of patients with carpal tunnel syndrome(CTS).Methods A total of 100 CTS patients who were admitted to The Second Affiliated Hospital of Naval Medical University from January 2022 to December 2023 were randomly assigned to observation group or control group,with 50 patients in each group.The control group was given a routine postoperative rehabilitation nursing,while the observation group was additionally given the"one disease,one product"rehabilitation nursing under follow-up care.The clinical efficacy,visual analogue scale(VAS)score,quality-of-life score,psychological status score(self-rating depression scale[SDS]and self-rating anxiety scale[SAS]),wrist recovery(grip strength,pinch strength,patient rated wrist evaluation[PRWE]score),and nursing satisfaction were compared between the two groups.Results The total effective rate in the observation group was significantly higher than that in the control group(98.00%[49/50]vs.86.00%[43/50],P<0.05).After treatment,VAS score,SDS score,and SAS score were decreased,and quality-of-life scores,grip strength,pinch strength,and PRWE score were increased in both groups;VAS score,SDS score,and SAS score in the observation group were significantly lower than those in the control group,while quality-of-life scores,grip strength,pinch strength,and PRWE score in the observation group were significantly higher than those in the control group(all P<0.05).Patient satisfaction in the observation group was significantly higher than that in the control group(98.00%[49/50]vs.88.00%[44/50],P<0.05).Conclusion The"one disease,one product"rehabilitation nursing program under the follow-up nursing mode has a better application effect than routine postoperative rehabilitation nursing plan in postoperative nursing of patients with CTS.

Objective:To evaluate the value of HALP index on postoperative survival of patients with distal cholangiocarcinoma.Methods:A retrospective analysis was performed on 165 patients with distal cholangiocarcinoma who underwent surgery at Beijing Chaoyang Hospital, Capital Medical University from Jan 2011 to Dec 2022. Hemoglobin, albumin, lymphocyte count, and platelet count were recorded within one week before surgery, and hemoglobin×albumin×lymphocyte count/platelet count (HALP) was calculated. The receiver operating characteristic curve was drawn to determine the optimal cutoff value for predicting postoperative survival. According to the cutoff value, the patients were divided into high HALP group and low HALP group, and the prognosis of different groups was compared. Multivariate COX regression analysis was used to construct a prediction model for variables with statistically significant differences.Results:The median overall survival (OS) for the entire cohort was 29 months, with cumulative 1-, 3-, and 5-year survival rates of 78.2%, 38.8%, and 30.4%, respectively. The low-HALP group (median OS: 22 months) had significantly inferior survival compared to the high-HALP group (median OS: 37 months), with 1-, 3-, and 5-year survival rates of 70.1%,31.4%,22.8% vs. 89.7%,45.3%,38.1% ( χ2=7.695, P=0.006). Conclusion:The HALP index calculated from the patient's preoperative indicators can be used to predict the overall survival of patients with distal cholangiocarcinoma.

Objective:To evaluate the value of HALP index on postoperative survival of patients with distal cholangiocarcinoma.Methods:A retrospective analysis was performed on 165 patients with distal cholangiocarcinoma who underwent surgery at Beijing Chaoyang Hospital, Capital Medical University from Jan 2011 to Dec 2022. Hemoglobin, albumin, lymphocyte count, and platelet count were recorded within one week before surgery, and hemoglobin×albumin×lymphocyte count/platelet count (HALP) was calculated. The receiver operating characteristic curve was drawn to determine the optimal cutoff value for predicting postoperative survival. According to the cutoff value, the patients were divided into high HALP group and low HALP group, and the prognosis of different groups was compared. Multivariate COX regression analysis was used to construct a prediction model for variables with statistically significant differences.Results:The median overall survival (OS) for the entire cohort was 29 months, with cumulative 1-, 3-, and 5-year survival rates of 78.2%, 38.8%, and 30.4%, respectively. The low-HALP group (median OS: 22 months) had significantly inferior survival compared to the high-HALP group (median OS: 37 months), with 1-, 3-, and 5-year survival rates of 70.1%,31.4%,22.8% vs. 89.7%,45.3%,38.1% ( χ2=7.695, P=0.006). Conclusion:The HALP index calculated from the patient's preoperative indicators can be used to predict the overall survival of patients with distal cholangiocarcinoma.

Posterior cruciate ligament (PCL) plays an important role in maintaining the stability of knee. PCL injury is often accompanied by serious axial and rotational instability, and severe PCL injury is likely to be combined with injuries to the anterior cruciate ligament, medial collateral ligament and other tissues which are often repaired by necessary posterior cruciate ligament reconstruction (PCLR) to restore their physiological functions. However, PCLR research is not as common as the research into the anterior cruciate ligament reconstruction, not only due to controversies in the anatomy and mechanics of PCL but also due to a higher failure rate and more complications following PCLR. This situation is closely related to the anatomical characteristics of the PCL tibial insertion. The present review deals with the anatomy, mechanics and clinical research of the PCL tibial insertion in order to provide more references for PCLR operators.

Objective:To evaluate the impact of neoadjuvant chemotherapy on long-term prognosis of patients with borderline resectable pancreatic cancer (BRPC) treated with combined allograft revascularization.Methods:The data of patients with BRCP who were treated at Beijing Chaoyang Hospital, Capital Medical University from March 2016 to March 2021 were retrospectively analysed. Of 52 patients who underwent radical surgery combined with allograft revascularization in this study, there were 24 males and 28 females, aged (60.3±10.6) years old. These patients were divided into two groups based on whether they received neoadjuvant chemotherapy before surgery. There were 19 patients in the neoadjuvant chemotherapy group and 33 patients in the vascular replacement group. Outpatient clinic and telephone follow-up were used. The clinical data and prognostic differences between the two groups were then analysed.Results:Of 52 patients who underwent surgery successfully, 14 patients (26.9%) developed postoperative complications. The incidence of postoperative pancreatic fistula was significantly lower in the neoadjuvant chemotherapy group than the vascular replacement group (0 vs. 21.2%, P<0.05). The median survivals were 15 and 13 months in the neoadjuvant chemotherapy and the vascular replacement groups, respectively, with a significant difference in cumulative postoperative survival between the two groups ( P=0.039). For patients with BRPC, CA19-9>400 U/ml ( RR=4.540, 95% CI: 2.332-8.836, P<0.001) was an independent risk factor for long-term survival after surgery. Conclusions:Neoadjuvant chemotherapy reduced the incidence of postoperative pancreatic fistula and improved survival prognosis in patients with BRPC. A high preoperative serum CA19-9 level was an independent risk factor for long-term survival in patients with BRPC.

Objective To study the effect of tiotropium on urination disorder in benign prostatic hyperplasia (BPH) patients with chronic obstructive pulmonary disease (COPD).Methods In our prospective pilot study,96 BPH patients with COPD patients were enrolled as the treatment group and another 25 similar cases as the control group:In the former group tiotropium was administered and the control group was not.The two groups were compared in terms of the score by the international Prostate Symptom Score(IPSS),the quality of life by QOL,maximum flow rate (Q-max),average flow rate (Q-ave),time to Q-max (TTQ-Max),prostate volume (PVR) and bladder voiding efficiency (BVE) after six months treatment.Results As compared to the control,after six months treatment,such indexes in the treatment group as IPSS (15.1 ± 4.1,16.3 ± 3.4 and 14.7 ± 3.1,P =0.864),QOL(3.9 ± 0.8,4.0± 0.8 and 4.0 ± 0.9,P =0.992),Q-Max(ml/s) (8.5 ± 2.9,10.9 ± 2.2 and 9.0 ± 2.4,P =0.214),Q-ave(ml/s) (3.9 ±1.2,5.0 ± 1.4 and 3.8 ± 0.9,P =0.054),TTQ-Max(s) (11.1 ± 5.6,11.2 ± 4.0 and 10.4 ± 5.1,P =0.424),PVR(mL)(56.8 ± 33.3,62.3 ± 30.5 and 57.4 ± 29.5,P =0.981),BVE(％) (75.6 ± 13.8,72.7 ± 10.5 and 74.3 ± 12.1,P =0.992).showed no significant differences.Conclusion Tiotropium does not adversely affect lower urinary tract functions in BPH patients with COPD.

A rapid and sensitive method for the determination of trace carbaryl in water by using diallyl phthalate-europium ( Eu3+) as fluorescent probes was developed. The interaction between Eu3+ and diallyl phthalate and carbaryl was studied by high resolution mass spectrum, and the fluorescence spectra change of complexes before/after binding with carbaryl was also investigated. The influence factors of fluorescence intensity including solution pH and interferent were studied. The results showed that two diallyl phthalate molecules were complexed with one Eu3+to form stable complexes. Carbaryl could also interact with the probe to form multiple complexes, which significantly increased the fluorescent efficiency of the probe. At pH 9. 0 of solution and by using 245/615 nm as excitation/emission wavelength, the fluorescence intensity showed good linear relationship with the carbaryl concentration ranged from 6. 25í10-8 mol/L to 2. 50í10-6 mol/L, and the linear correlation coefficient was 0. 9968. The detection limit of the method was 9. 6í10-9 mol/L. Water samples were extracted by acetonitrile, and then detected by europium ( Eu3+)-diallyl phthalate fluorescent probe. The recovery of the method was 91. 8%-94. 5%, while RSD was within 6. 1%. The method is suitable for the rapid determination of carbaryl in water samples.

AIM: To investigate whether salidroside has influence on the activities of endothelial progenitor cells (EPCs) and its mechanism.METHODS:Mononuclear cells from normal human peripheral blood were cultured in fi-bronectin coated flasks in endothelial progenitor medium .After 7 d, EPCs were characterized as adherent cells with acLDL-DiI uptaking and lectin binding by direct fluorescent staining .The proliferation and migration of EPCs were analyzed by MTT assay and Transwell chamber assay , respectively.The EPCs adhesion assay was performed by re-plating the cells on fibronectin-coated dishes , and then adherent cells were counted .NO and Akt protein were also detected .RESULTS:Sali-droside promoted EPCs proliferative , migratory and adhesive capacities in a concentration dependent manner .Salidroside also increased NO secretion , and the level of phosphorylated Akt protein .However , the effects of salidroside on EPCs were inhibited by phosphoinositide 3-kinase inhibitor LY294002.CONCLUSION:Salidroside regulates the activity of EPCs by phosphoinositide 3-kinase/Akt signaling pathway .

The crystal form of solid substance had intrinsic correlation with its three dimensional crystal morphology. Based on the characterization of the three dimensional crystal morphology of clopidogrel bisulfate, this research is to establish a model based on the three dimensional morphological parameters. The granular samples composed of polymorphs of clopidogrel bisulfate and microcrystalline cellulose (MCC) were scanned by synchrotron radiation X-ray microscopic CT technology (SR-microCT) and the three dimensional structural models for which were constructed. Seven groups of three dimensional morphological parameters were calculated. Finally, the mathematical model was established with the multi-layer perception (MLP) artificial neutral network methods to identify and predict the polymorphs of clopidogrel bisulfate. The success rate of the model prediction for the polymorphs of clopidogrel bisulfate was 92.7% and the area under the ROC curve was 96.2%. The polymorphs of drugs could be identified and predicted through the numerical description of the three dimensional morphology. The volume, number of the vertices and the surface area were the major determinants for the identification of the polymorphs of clopidogrel bisulfate.