Objective To analyze the changes in optical coherence tomography angiography (OCTA) parameters in patients with essential hypertension，and to explore the effect of blood pressure on OCTA parameters. Methods A total of 164 patients with essential hypertension were selected and divided into controlled blood pressure group (n=92) and uncontrolled blood pressure group (n=72). OCTA examination was performed on the optic disc and macula of all patients, and the right eyes were selected for analysis. Results There were no significant differences in retinal nerve fiber layer (RNFL) thickness, radial peripapillary capillary (RPC) total vascular density, RPC total small vessel density, perifovea superficial capillary plexus (SCP) vascular density, and perifovea deep capillary plexus (DCP) vascular density between the two groups of patients. There were no significant differences in foveal avascular zone (FAZ) area, FAZ diameter, and fovea retinal thickness between the two groups of patients. The density of the parafovea SCP, parafovea DCP, and fractal dimension (FD) in the uncontrolled blood pressure group were significantly lower than those in the controlled blood pressure group (P＜0.05). Multiple linear regression analysis showed that elevation of blood pressure was a independently related factor of reduced parafovea DCP density (P＝0.026), while there was no correlation between the uncontrolled blood pressure and parafovea SCP density and FD level. Conclusions The blood pressure level is correlated with the parafovea DCP density, while has no correlation with other OCTA parameters in hypertension patients.

Atherosclerosis, a chronic inflammatory disease, is markedly influenced by both immune and inflammatory reactions throughout its progression. Dendritic cells, as pivotal antigen-presenting entities, play a crucial role in the initiation of immune responses and the preservation of immunological homeostasis. Accumulating data indicates that dendritic cells are present in healthy arteries and accumulate significantly in atherosclerotic plaques. Novel immunotherapeutic approaches and vaccination protocols have yielded substantial clinical advancements in managing chronic inflammatory diseases, with dendritic cell-centric modalities emerging for atherosclerotic management. In this review, we delineate the essential functions and underlying mechanisms of dendritic cells and their subsets in the modulation of atherosclerotic inflammation and immune responses. We underscore the immense promise of dendritic cell-based immunotherapeutic strategies, including vaccines and innovative combinations with nanotechnological drug delivery platforms for atherosclerosis treatment. We also discuss the challenges associated with dendritic cell immunotherapy and provide perspectives on the future direction of this field.

Objective:To construct three photosensitive fusion proteins between protein G C3 domain and Avitag for site-spe-cific biotinylation of goat IgG.Methods:DNA sequences of protein G C3 domain with amber codon mutation and C-terminal containing 1,2,3 Avitag were obtained by chemical synthesis,respectively,and sub-cloned into pTXB1 plasmid to construct three fusion pro-tein expression vectors.Three plasmids and pEVOL-pBpF plasmid were co-transformed into competent bacterium E.coli BL21,respec-tively.Target fusion proteins CBpa-Avitag,CBpa-(Avitag)2 and CBpa-(Avitag)3 were prepared using genetic codon extension technology,and were purified by His Trap Chelating HP column,then biotinylated by Avitag/BirA system.Photo-conjugation efficiency,crosslink site and antigen-binding activity of photo-conjugates were investigated by SDS-PAGE,control experiment,and competitive ELISA.Results:Three expression vectors were successfully constructed,and three designed photoactivatable fusion proteins CBpa-Avitag,CBpa-(Avitag)2 and CBpa-(Avitag)3 were successfully expressed with molecular weights consistent with their theoretical values.Heavy-chain conjugation efficiencies of biotinylated fusion protein with goat IgG were 85%with Fc-specific conjugation,and conjugates maintained its antigen-binding activity after photo-conjugation.Conclusion:CBpa-Avitag,CBpa-(Avitag)2 and CBpa-(Avitag)3 fusion proteins are successfully prepared,and mediates by those photosensitive fusion proteins,biotins molecules are conjugated to Fc fragment of goat IgG,results site-specific biotinylation of goat IgG.

Objective:To construct three photosensitive fusion proteins between protein G C3 domain and Avitag for site-spe-cific biotinylation of goat IgG.Methods:DNA sequences of protein G C3 domain with amber codon mutation and C-terminal containing 1,2,3 Avitag were obtained by chemical synthesis,respectively,and sub-cloned into pTXB1 plasmid to construct three fusion pro-tein expression vectors.Three plasmids and pEVOL-pBpF plasmid were co-transformed into competent bacterium E.coli BL21,respec-tively.Target fusion proteins CBpa-Avitag,CBpa-(Avitag)2 and CBpa-(Avitag)3 were prepared using genetic codon extension technology,and were purified by His Trap Chelating HP column,then biotinylated by Avitag/BirA system.Photo-conjugation efficiency,crosslink site and antigen-binding activity of photo-conjugates were investigated by SDS-PAGE,control experiment,and competitive ELISA.Results:Three expression vectors were successfully constructed,and three designed photoactivatable fusion proteins CBpa-Avitag,CBpa-(Avitag)2 and CBpa-(Avitag)3 were successfully expressed with molecular weights consistent with their theoretical values.Heavy-chain conjugation efficiencies of biotinylated fusion protein with goat IgG were 85%with Fc-specific conjugation,and conjugates maintained its antigen-binding activity after photo-conjugation.Conclusion:CBpa-Avitag,CBpa-(Avitag)2 and CBpa-(Avitag)3 fusion proteins are successfully prepared,and mediates by those photosensitive fusion proteins,biotins molecules are conjugated to Fc fragment of goat IgG,results site-specific biotinylation of goat IgG.

Affiliated Beijing Chaoyang Hospital of Capital Medical University focus on problem solving.By applying the dual responsibility for one position system and compacting the main responsibility of party and government leaders,the hospital aims to achieve organic integration of party building and business organic integration of party building and businessat the organizational management level.By emphasizing on adopting systematic thinking method and establishing modernized hospital management systems,the hospital aims to achieve integration at the institutional system level.Adhering to the joint efforts of the party building and administrative work and focusing on improving comprehensive service quality,the hospital aims to achieve integration at the behavioral level.By valuing ideological integration and guiding hospital cultural construction with strict political standards,the hospital aims to achieve integration at the cultural level.Through systematic organizational reformation,a new approach to the organismic integration of party building and business in public hospitals has been explored,and a new mechanism with innovative vitality and effective results has been initially formed.

Affiliated Beijing Chaoyang Hospital of Capital Medical University focus on problem solving.By applying the dual responsibility for one position system and compacting the main responsibility of party and government leaders,the hospital aims to achieve organic integration of party building and business organic integration of party building and businessat the organizational management level.By emphasizing on adopting systematic thinking method and establishing modernized hospital management systems,the hospital aims to achieve integration at the institutional system level.Adhering to the joint efforts of the party building and administrative work and focusing on improving comprehensive service quality,the hospital aims to achieve integration at the behavioral level.By valuing ideological integration and guiding hospital cultural construction with strict political standards,the hospital aims to achieve integration at the cultural level.Through systematic organizational reformation,a new approach to the organismic integration of party building and business in public hospitals has been explored,and a new mechanism with innovative vitality and effective results has been initially formed.

Affiliated Beijing Chaoyang Hospital of Capital Medical University focus on problem solving.By applying the dual responsibility for one position system and compacting the main responsibility of party and government leaders,the hospital aims to achieve organic integration of party building and business organic integration of party building and businessat the organizational management level.By emphasizing on adopting systematic thinking method and establishing modernized hospital management systems,the hospital aims to achieve integration at the institutional system level.Adhering to the joint efforts of the party building and administrative work and focusing on improving comprehensive service quality,the hospital aims to achieve integration at the behavioral level.By valuing ideological integration and guiding hospital cultural construction with strict political standards,the hospital aims to achieve integration at the cultural level.Through systematic organizational reformation,a new approach to the organismic integration of party building and business in public hospitals has been explored,and a new mechanism with innovative vitality and effective results has been initially formed.

Affiliated Beijing Chaoyang Hospital of Capital Medical University focus on problem solving.By applying the dual responsibility for one position system and compacting the main responsibility of party and government leaders,the hospital aims to achieve organic integration of party building and business organic integration of party building and businessat the organizational management level.By emphasizing on adopting systematic thinking method and establishing modernized hospital management systems,the hospital aims to achieve integration at the institutional system level.Adhering to the joint efforts of the party building and administrative work and focusing on improving comprehensive service quality,the hospital aims to achieve integration at the behavioral level.By valuing ideological integration and guiding hospital cultural construction with strict political standards,the hospital aims to achieve integration at the cultural level.Through systematic organizational reformation,a new approach to the organismic integration of party building and business in public hospitals has been explored,and a new mechanism with innovative vitality and effective results has been initially formed.

Affiliated Beijing Chaoyang Hospital of Capital Medical University focus on problem solving.By applying the dual responsibility for one position system and compacting the main responsibility of party and government leaders,the hospital aims to achieve organic integration of party building and business organic integration of party building and businessat the organizational management level.By emphasizing on adopting systematic thinking method and establishing modernized hospital management systems,the hospital aims to achieve integration at the institutional system level.Adhering to the joint efforts of the party building and administrative work and focusing on improving comprehensive service quality,the hospital aims to achieve integration at the behavioral level.By valuing ideological integration and guiding hospital cultural construction with strict political standards,the hospital aims to achieve integration at the cultural level.Through systematic organizational reformation,a new approach to the organismic integration of party building and business in public hospitals has been explored,and a new mechanism with innovative vitality and effective results has been initially formed.

Affiliated Beijing Chaoyang Hospital of Capital Medical University focus on problem solving.By applying the dual responsibility for one position system and compacting the main responsibility of party and government leaders,the hospital aims to achieve organic integration of party building and business organic integration of party building and businessat the organizational management level.By emphasizing on adopting systematic thinking method and establishing modernized hospital management systems,the hospital aims to achieve integration at the institutional system level.Adhering to the joint efforts of the party building and administrative work and focusing on improving comprehensive service quality,the hospital aims to achieve integration at the behavioral level.By valuing ideological integration and guiding hospital cultural construction with strict political standards,the hospital aims to achieve integration at the cultural level.Through systematic organizational reformation,a new approach to the organismic integration of party building and business in public hospitals has been explored,and a new mechanism with innovative vitality and effective results has been initially formed.