Aim To investigate the effect of adenovirus(ADV)-mediated Profurin(PF)expression on the plaque stability of ApoE-/-mice.Methods ApoE-/-mice were fed with high-fat diet for 8 weeks,and then treated with ADV-mediated PF intervention,followed by high-fat diet for 4 weeks.Aortic roots were isolated for atherosclerotic plaque area analysis and immunohistochemical analysis.Plasma phospholipid transfer protein(PLTP)activity was detec-ted by fluorescence donor essay,plasma total cholesterol(TC)and triglyceride(TG)were measured by enzyme assay kits,and fast protein liquid chromatography was used for lipoprotein profile analysis.Results Compared with the con-trol group,the plasma TC and TG levels,PLTP activity and circulating tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)levels in ADV-PF group were significantly decreased(P＜0.01).In the ADV-PF group,there was no significant change in atherosclerotic lesions on the inner surface of the full-length aorta,but the plaque area and lipid area in the aortic root were reduced(P＜0.01),the content of macrophages was significantly decreased(P＜0.01),and the smooth muscle cells and collagen area were not significantly different.The content of matrix metalloproteinase-9 in plaque was signifi-cantly decreased(P＜0.05).Conclusion Overexpression of PF can alleviate atherosclerosis and reduce the levels of circulating inflammatory factors to a certain extent,and effectively improve the plaque stability of ApoE-/-mice.

The theory of the "Three Phases of moxibustion sensation", originating from ZHOU Meisheng's Moxibustion Cord, summarizes the clinical transmission process of moxibustion sensation into three phases: initial sensation, diffusion, and conduction. Inspired by this theory, the authors analyze factors affecting moxibustion sensation-including moxibustion temperature, location, dose, and technique-and summarize its guiding role in clinical practice. It is proposed that in clinical application, the generation pattern of the initial sensation phase can be used to judge the immediate efficacy of moxibustion and adjust treatment locations accordingly; the diffusion pattern of the diffusion phase can help assess individual moxibustion dose to ensure optimal therapeutic effects; and the conduction phase not only indicates the endpoint of moxibustion therapy but can also reveal hidden lesions and deeper pathological causes in patients. In addition, due to differences in patient constitution and disease state, the manifestation of moxibustion sensation may not always strictly follow the pattern of the "Three Phases", requiring careful clinical differentiation.
Moxibustion/methods* ; Humans ; Sensation ; Acupuncture Points

Objective:To investigate the efficacy of compression fixation with cross-locking Kirschner wires in the treatment of bicondylar fractures of the phalangeal neck in fingers (toes).Methods:A retrospective study was conducted to analyze the clinical data of 15 patients with bicondylar fractures of the phalangeal neck in fingers (toes) who had been treated from February 2019 to August 2023 at Department of Hand and Foot Surgery, Taizhou Hospital. The patients were all treated by compression fixation with cross-locking Kirschner wires. They were 13 males and 2 females with an age of (43.4±11.0) years and with 9 hands and 6 feet involved. In 2 of them, the fracture line of the bone block was completely located far from the insertion point of the lateral collateral ligament (condylar lateral recess). After surgery, no additional external fixation was used for all but 2 patients whose bone fold line was fixed with plaster for 3 to 4 weeks at a distance from the lateral collateral ligament insertion point. Follow-up records included fracture healing time, visual analogue scale (VAS) pain score at postoperative 3 months, total active motion (TAM) of the finger for hand patients at the final follow-up, and surgical complications.Results:All patients were followed up for (5.4±3.0) months. The VAS pain score for all patients was 0 (0, 0) point at postoperative 3 months. Only 2 patients reported mild pain in the hand at 3 months postoperatively (their VAS pain score was 1 or 2 respectively). At 12-month follow-up, 1 foot patient reported mild pain (1 point of VAS) and the other 5 foot patients experienced no pain while they were carrying weights and walking normally. X-ray evaluations at postoperative 4, 6, and 8 weeks showed nonunion of a middle phalanx intra-articular fracture of the ring finger in 1 case, complicated by bone resorption and joint stiffness which were not further treated. The other cases achieved bony union after an average healing time of (7.1±1.9) weeks. Of the 8 hand patients whose total active motion was measured at the final follow-up using the TAM scoring system, 6 were rated as excellent and 2 as good. No fracture displacement, loosening or withdrawal of Kirschner wires, or no needle tunnel infection occurred.Conclusion:In the treatment of bicondylar fractures of the phalangeal neck in fingers (toes), compression fixation with cross-locking Kirschner wires offers advantages of simplicity in operation, minimal invasiveness, good stability, and facilitation of early postoperative rehabilitation.

Environmental factors such as air pollutants,plastic micro-particles,ionizing radiation,and traffic noise promote the onset and progression of atherosclerotic cardiovascular disease(CVD)through mechanisms including inducing inflammatory responses,promoting oxidative stress,and accelerating lipid deposition.Notably,the modification of apoli-poprotein(Apo)by reactive oxygen species(ROS)accelerates this process.On one hand,ROS can oxidize key amino acid residues in Apo,altering its structure and thereby impairing its normal physiological function.On the other hand,ROS can induce lipid peroxidation of Apo,rendering the oxidized Apo more susceptible to recognition and uptake by macro-phages.This accelerates foam cell formation and drives the progression of atherosclerotic(As)plaques.This review summarizes recent research advances on the role of ROS-induced modification of Apo by environmental factors in atheroscle-rotic CVD.

Environmental factors such as air pollutants,plastic micro-particles,ionizing radiation,and traffic noise promote the onset and progression of atherosclerotic cardiovascular disease(CVD)through mechanisms including inducing inflammatory responses,promoting oxidative stress,and accelerating lipid deposition.Notably,the modification of apoli-poprotein(Apo)by reactive oxygen species(ROS)accelerates this process.On one hand,ROS can oxidize key amino acid residues in Apo,altering its structure and thereby impairing its normal physiological function.On the other hand,ROS can induce lipid peroxidation of Apo,rendering the oxidized Apo more susceptible to recognition and uptake by macro-phages.This accelerates foam cell formation and drives the progression of atherosclerotic(As)plaques.This review summarizes recent research advances on the role of ROS-induced modification of Apo by environmental factors in atheroscle-rotic CVD.

Aim To investigate the effect of adenovirus(ADV)-mediated Profurin(PF)expression on the plaque stability of ApoE-/-mice.Methods ApoE-/-mice were fed with high-fat diet for 8 weeks,and then treated with ADV-mediated PF intervention,followed by high-fat diet for 4 weeks.Aortic roots were isolated for atherosclerotic plaque area analysis and immunohistochemical analysis.Plasma phospholipid transfer protein(PLTP)activity was detec-ted by fluorescence donor essay,plasma total cholesterol(TC)and triglyceride(TG)were measured by enzyme assay kits,and fast protein liquid chromatography was used for lipoprotein profile analysis.Results Compared with the con-trol group,the plasma TC and TG levels,PLTP activity and circulating tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)levels in ADV-PF group were significantly decreased(P＜0.01).In the ADV-PF group,there was no significant change in atherosclerotic lesions on the inner surface of the full-length aorta,but the plaque area and lipid area in the aortic root were reduced(P＜0.01),the content of macrophages was significantly decreased(P＜0.01),and the smooth muscle cells and collagen area were not significantly different.The content of matrix metalloproteinase-9 in plaque was signifi-cantly decreased(P＜0.05).Conclusion Overexpression of PF can alleviate atherosclerosis and reduce the levels of circulating inflammatory factors to a certain extent,and effectively improve the plaque stability of ApoE-/-mice.

Objective:To investigate the efficacy of compression fixation with cross-locking Kirschner wires in the treatment of bicondylar fractures of the phalangeal neck in fingers (toes).Methods:A retrospective study was conducted to analyze the clinical data of 15 patients with bicondylar fractures of the phalangeal neck in fingers (toes) who had been treated from February 2019 to August 2023 at Department of Hand and Foot Surgery, Taizhou Hospital. The patients were all treated by compression fixation with cross-locking Kirschner wires. They were 13 males and 2 females with an age of (43.4±11.0) years and with 9 hands and 6 feet involved. In 2 of them, the fracture line of the bone block was completely located far from the insertion point of the lateral collateral ligament (condylar lateral recess). After surgery, no additional external fixation was used for all but 2 patients whose bone fold line was fixed with plaster for 3 to 4 weeks at a distance from the lateral collateral ligament insertion point. Follow-up records included fracture healing time, visual analogue scale (VAS) pain score at postoperative 3 months, total active motion (TAM) of the finger for hand patients at the final follow-up, and surgical complications.Results:All patients were followed up for (5.4±3.0) months. The VAS pain score for all patients was 0 (0, 0) point at postoperative 3 months. Only 2 patients reported mild pain in the hand at 3 months postoperatively (their VAS pain score was 1 or 2 respectively). At 12-month follow-up, 1 foot patient reported mild pain (1 point of VAS) and the other 5 foot patients experienced no pain while they were carrying weights and walking normally. X-ray evaluations at postoperative 4, 6, and 8 weeks showed nonunion of a middle phalanx intra-articular fracture of the ring finger in 1 case, complicated by bone resorption and joint stiffness which were not further treated. The other cases achieved bony union after an average healing time of (7.1±1.9) weeks. Of the 8 hand patients whose total active motion was measured at the final follow-up using the TAM scoring system, 6 were rated as excellent and 2 as good. No fracture displacement, loosening or withdrawal of Kirschner wires, or no needle tunnel infection occurred.Conclusion:In the treatment of bicondylar fractures of the phalangeal neck in fingers (toes), compression fixation with cross-locking Kirschner wires offers advantages of simplicity in operation, minimal invasiveness, good stability, and facilitation of early postoperative rehabilitation.

Objective:To analyze the medication law of TCM oral prescriptions in the treatment of postherpetic neuralgia (PHN).Methods:The effective cases and clinical studies about TCM oral prescriptions in the treatment of PHN were retrieved from CNKI, Wanfang Data, VIP, and CBM from January 1, 2002 to December 31, 2021. The medication frequency and drug properties were counted through the Ancient and Modern Medical Record Cloud Platform, and correlation analysis, cluster analysis and complex network analysis were performed.Results:A total of 233 articles were included, involving 243 prescriptions and 191 kinds of Chinese materia medica. There were 20 kinds of Chinese materia medica with frequency >40, most of which have the efficacy of promoting blood circulation to remove blood stasis. The Chinese materia with heat-clearing and detoxifying efficacy were also often used. The properties were mainly warm, mild, slight cold, and cold, tastes were mainly bitter, pungent and sweet, and meridians were mainly liver, spleen and heart meridians. The association rule analysis was used to obtain 15 commonly used TCM groups. The commonly used Chinese materia medica could be clustered into 4 categories. The core prescription obtained by complex network analysis was Angelicae Sinensis Radix, Glycyrrhizae Radix et Rhizoma, Corydalis Rhizoma, Bupleuri Radix, Carthami Flos, Paeoniae Radix Alba, Persicae Semen, Chuanxiong Rhizoma, and Rehmanniae Radix.Conclusion:TCM oral prescriptions for the treatment of PHN put promoting blood circulation to remove blood stasis and activating meridians to stop pain in priority, taking into account the clearing heat, regulating qi and blood.

Objective:To summarize the clinical and genetic characteristics of children with epilepsy associated with SCN2A gene variations. Methods:A retrospective study was performed. Eleven children with epilepsy admitted to Department of Pediatric Neurology, Linyi People's Hospital from January 2017 to December 2022 were included; all of them had pathogenic SCN2A gene mutation. Genetic results and clinical data as epileptic seizure type/frequency, intelligence and motor development of these 11 children were collected. Epilepsy-related variations and pathogenesis of SCN2A gene were analyzed, and their correlations with clinical phenotypes in these children were analyzed. Results:Among the 11 patients, 6 had self-limited epilepsy (4 with variation in the intracellular domain and 2 in the transmembrane domain), 1 had febrile convulsion accompanied by childhood absent epilepsy (with variation in the intracellular domain), and 4 had developmental epileptic encephalopathy (2 with variation in the extracellular domain and 2 with variation in the transmembrane domain). SCN2A gene was missense mutation in these 11 children, and the mutation site in 6 children was not reported before. Various forms of video EEG discharge were noted, and 1 child with self-limited epilepsy showed transient multifocal epileptic discharge during frequent seizures. Oxcarbazepine and topiramate were effective for self-limiting epilepsy, and lamotrigine was effective in 1 child with late-onset epileptic encephalopathy. Eleven patients were followed up for (66±32) months; the age ranged from 8 months to 11 years and 6 months at the last follow-up; 10 patients had seizure remission and 1 had uncontrolled seizure. Conclusions:Besides self-limited epilepsy and developmental epileptic encephalopathy, SCN2A gene mutations are also associated with febrile convulsion and childhood absent epilepsy. Phenotypic differences are highly correlated with mutation locations; developmental epileptic encephalopathy associated variants are mostly located in extracellular domains, while self-limited epileptic variants are mostly located in intracellular domains.

Objective:By exploring the source of children's total fluoride intake and the relationship between children's total fluoride intake and dental fluorosis prevalence, to calculate the benchmark dose (BMD) of children's total fluoride intake and its 95% confidence lower limit (BMDL), and to provide the basis for revision of "Total Fluoride Intake for Inhabitants" (WS/T 87-2016).Methods:The villages that had water improvement for 5 years and more in 6 provinces of Jiangsu, Shandong, Hebei, Anhui, Henan and Shaanxi were selected as survey sites in April 2014. The water fluoride content of these villages was 0.3 - 3.0 mg/L, tap water samples from the centralized water supply were collected, and fluoride content was detected by ion selective electrode method. Children aged 8 to 12 years were selected, children's dental fluorosis was checked by Dean's method. Children's dietary and drinking water volume were surveyed by duplicate portion study (measurement for 3 d), and dietary fluoride content was detected according to the "Method for Determination of Fluorine in Foods". The mean and standard deviation of drinking water fluoride intake, dietary fluoride intake, and total fluoride intake were measured. According to the dose-response relationship between children's total fluoride intake and the detection rate of dental fluorosis, BMD and BMDL were calculated, and the reference dose (RfD) was calculated based on BMDL.Results:The mean of water fluoride of all 29 villages was 1.26 mg/L (from 0.41 to 2.85 mg/L). Totally 3 043 children aged 8 to 12 years were checked, and the detection rate of dental fluorosis was 30.2% (919/3 034). The lowest detection rate of dental fluorosis was 2.0% (2/100) and the highest was 71.4% (30/42) in the 29 villages. The children's dietary and drinking water volume of 769 person-time aged 8 to 12 years were surveyed, the children's daily drinking water fluoride intake, dietary fluoride intake, and total fluoride intake were (0.83 ± 0.66), (1.13 ± 0.61) and (1.96 ± 0.89) mg/d, respectively. The BMD of children's daily total fluoride intake was 2.43 mg, the BMDL was 2.21 mg, and the RfD was 2.21 mg.Conclusion:The BMD of 8 to 12 years old children's daily total fluoride intake is the same as the allowable limit (2.4 mg) of the national standard "Total Fluoride Intake for Inhabitants" (WS/T 87-2016).