Objective:To establish the fingerprint analysis method for the root of Rosa laevigata Michx from different regions by UPLC. Methods:The column was ACQUITY UPLC? Phenyl(2.1 mm × 100 mm,1.7 μm). The mobile phase consisted of methanol-water with gradient elution. The flow rate was 0. 2 ml·min-1 , the detection wavelength was 210 nm, the column temperature was 30℃, and the injection volume was 3 μl. Results:The fingerprint consisted of 15 common peaks. The range of similarity for twelve bat-ches of the root of R. laevigata Michx was 0. 489-0. 942. And the reference fingerprint of the root of R. laevigata Michx was estab-lished by UPLC. Conclusion:The fingerprint method is simple and reproducible, which can provide basis for the quality control and the medicinal resources exploration.

OBJECTIVETo develop an HPLC method for quantitative determination of three quassinoids in Brucea javanic.

METHODThe determination was carried out on a phenomenex C18 column (4.6 mm x 250 mm, 5 microm) with gradient elution program of methol-water at a flow rate of 1.0 mL x min(-1), and the detection wavelength was 270 nm.

RESULTLinearites of bruceine D, brusatol and bruceine H were good (r = 0.9996, 0.9996, and 0.9998) in ranges of 2.52-12.60, 2.19-10.95, and 2.91-14.55 microg, respectively. The average recoveries of bruceine D, brusatol and bruceine H were 100.01%, 100.95% and 100.43% respectively, and RSD of the above three compounds were 0.31% (n = 6), 1.7% ( n = 6) and 1.7% (n = 6), respectively.

CONCLUSIONThe determination results of three batch samples showed that the method was simple, accurate and could be used in the quantitative determination of three components in the B. javanica.

Brucea ; chemistry ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; isolation & purification ; standards ; Linear Models ; Organic Chemicals ; analysis ; isolation & purification ; standards ; Quality Control

Object To develop a resonance scattering spectral(RSS) method for the determination of total flavone in Shuxuening Tablet, and study the cause of RSS enhancement. Methods The BSA Mo (Ⅵ) qucercetin (Qu) system was investigated by RSS method. Results There were two resonance sacttering peaks at 470 and 525 nm. The Qu concentration in the range of 0-2 4 mg/L is linear to the resonance scattering intensity I 470 nm , with a detection limit of 0 3 mg/L. Conclusion This method is simple, sensitive and suitable for the determination of total flavone in Shuxuening Tablet. The spectral results demonstrate that the formation of [BSA Mo Qu] n associated particle and the interface between the particle solid phase and water phase results to its enhanced RSS.

Object To study the application of macroporous resins to the purified active components of Picria fel-terrae Lour., Scutellaria baicalensis Georgi, and Phellodendron chinense Schneid. Methods With the purified active components of Chinese materia medica (CMM) as a standard, we selected the suitable macroporous resins and studied the optimum technological parameters of the adsorption and elution. Results The suitable three types of macroporous resins which were used to the purified active components of CMM were HPD500 for P. fel-terrae., HPD300 for S. baicalensis, and HPD100 for P. chinense. In the absorption course, when the ratios between the medicinal materials amount and the volume of macroporous resins were 1.5 for P. fel-terrae, 0.5 for S. baicalensis and 1 for P. chinense, the purified active components of CMM appeared leaking, and were no more absorbed when the ratios were 11 for P. fel-terrae, 2 for S. baicalensis and 4.75 for P. chinense. In the elution course, the optimum alcohol concentrations were 50% for P. fel-terrae, 30% for S. baicalensis and 50% for P. chinense. Conclusion It is obviously different to refine the active components of CMM, while using the diverse types of macroporous resins.

Object To inquire into the effect on pharmacology of Gejie Dingchuan Capsule (GJDCC). Methods The observation of relieving cough and asthma, removing phlegm, immunity, antibiosis and antiinflammation, antianaphylaxis, and acute and long-term toxicity was carried out. Results GJDCC could resist the convulsion of the isolated trachea of guinea pigs, lengthen the asthma incubation period of guinea pigs, increase the phlegm liquid secreting capacity of isolated rat trachea, promote the pigeon trachea cilium motion, lengthen the mice cough incubation period, restrain the mice ear swelling and rat swelling hyperplasia of granulation, promote the mice producing of serum hemolysin and the lymphocyte conversion rate, and reduce the guinea pig allergic reaction index and shock death rate. It possessed the bacteriostasis, the biggest capacity of bearing consumption was 500 times the clinical daily use and in long-term toxicity test there were not the clear toxicity reactions. Conclusion GJDCC has the function of relieving asthma and cough, removing phlegm, antibiosis and antiinflammation, antianaphylaxis and immunity, without poisonous side effect.