As a new manner of cell death,cuproptosis depends on the accumulation of copper ions in cells.Copper ion is an essential trace element in normal physiological state of organisms.The excess of free copper in cells not only has toxic effect on normal cells,but also plays its specific killing function on tumor cells.Copper transporter 1(CTR1)is a key transporter of transmembrane uptake of copper ions by cells.As a regulator of cuproptosis,its mutation and expression changes in tumors have an impact on the distribution of copper ions inside and outside the cells.It may participate in multiple biological processes such as proliferation,invasion and migration of tumor cells by regulating the pathway of cuproptosis.This article reviews the cuproptosis pathway mediated by CTR1 and the potential value of CTR1 in tumor treatment,elaborates the importance of copper ion homeostasis regulation for normal life activities and the mechanism of CTR1 in regulating cuproptosis,and discusses the potential value of CTR1 as a new target for tumor therapy,so as to provide a theoretical basis for the treatment of tumor patients.

In recent years, artificial intelligence (AI) technology has seen widespread application in the field of healthcare, particularly revolutionizing disease diagnosis and treatment decisions. Assisted reproductive technology (ART), a crucial method for treating infertility, has also benefited from the integration of AI, especially in the intelligent development of its core process--controlled ovarian stimulation (COS). Traditional COS protocols heavily relied on the experience and subjective judgment of physicians, leading to uncertainties. However, AI technology leverages deep learning to analyze multi-dimensional data, including patients' demographic characteristics, reproductive endocrine levels, and ultrasound monitoring results, to provide precise, personalized optimization and dynamic adjustments for COS. Specifically, AI models can accurately calculate the initial COS dosage, intelligently monitor follicular development, and predict the optimal timing for ovulation triggering in real-time, significantly enhancing diagnostic and treatment efficiency, reducing the workload of physicians, and offering more individualized and precise treatment plans for patients. This article reviews the latest research progress in AI applications for individualized optimization of initial gonadotropin dosage during COS, intelligent follicular monitoring, assessment of ovarian responsiveness, and prediction of the optimal timing for ovulation triggering, aiming to provide valuable insights for the clinical practice of AI in assisted reproductive hyperstimulation.

In recent years, artificial intelligence (AI) technology has seen widespread application in the field of healthcare, particularly revolutionizing disease diagnosis and treatment decisions. Assisted reproductive technology (ART), a crucial method for treating infertility, has also benefited from the integration of AI, especially in the intelligent development of its core process--controlled ovarian stimulation (COS). Traditional COS protocols heavily relied on the experience and subjective judgment of physicians, leading to uncertainties. However, AI technology leverages deep learning to analyze multi-dimensional data, including patients' demographic characteristics, reproductive endocrine levels, and ultrasound monitoring results, to provide precise, personalized optimization and dynamic adjustments for COS. Specifically, AI models can accurately calculate the initial COS dosage, intelligently monitor follicular development, and predict the optimal timing for ovulation triggering in real-time, significantly enhancing diagnostic and treatment efficiency, reducing the workload of physicians, and offering more individualized and precise treatment plans for patients. This article reviews the latest research progress in AI applications for individualized optimization of initial gonadotropin dosage during COS, intelligent follicular monitoring, assessment of ovarian responsiveness, and prediction of the optimal timing for ovulation triggering, aiming to provide valuable insights for the clinical practice of AI in assisted reproductive hyperstimulation.

Objective To explore the relationship between lacune of presumed vascular origin and spontaneous HT in elderly patients with AIS.Methods A total of 671 AIS patients hospitalized in our hospital from January 2021 to September 2024 were prospectively recruited.According to the conditions of lacune of presumed vascular origin by the first cranial MRI examination after admis-sion,they were divided into 330 cases with lacune of presumed vascular origin and 341 cases with-out,and based on the re-examination of head CT or MRI during hospitalization,they were also as-signed into a HT group(139 cases)and a non-HT group(532 cases).Multivariate logistic regres-sion analysis was conducted to clarify the correlation between lacune of presumed vascular origin and spontaneous HT in the elderly AIS patients.The incidence of spontaneous HT and the differ-ences in the subtype composition of spontaneous HT were compared between the mild and severe groups of lacune of presumed vascular origin.Results Baseline NIHSS score,atrial fibrillation,white matter lesions,enlarged perivascular spaces,cerebral microbleeds,large-area cerebral infarc-tion,and ratio of lacune of presumed vascular origin were significantly higher in the HT group than the non-HT group[(10(6,13)vs 9(5,12),P=0.000;28.1％vs 18.0％,P=0.009;74.8％vs 56.0％,P=0.000;79.9％vs 57.5％,P=0.000;30.2％vs 16.9％,P=0.000;45.3％vs 13.2％,P=0.000;66.2％vs 44.7％,P=0.000].The incidence of spontaneous HT was significantly high-er in the lacune of presumed vascular origin group than the non-lacune of presumed vascular ori-gin group(27.9％vs 13.8％,P=0.000).Multivariate logistic regression analysis indicated that lacune of presumed vascular origin was an independent risk factor for spontaneous HT after AIS(OR=2.207,95％CI:1.231-3.959).Subtype analysis on spontaneous HT showed that the patients with lacune of presumed vascular origin had the largest proportion among all subtypes than the non-lacune of presumed vascular origin patients.The incidence of spontaneous HT was significantly higher in the severe lacune of presumed vascular origin group than the mild group(38.1％vs 20.9％,P=0.001).HI2 subtype was mainly observed in the severe group with sponta-neous HT,and HI1 subtype was in the mild group with spontaneous HT.Conclusion Lacune of presumed vascular origin is an independent risk factor for spontaneous HT after AIS in elderly patients.The patients with severe lacune of presumed vascular origin are prone to the occurrence and severe spontaneous HT than the mild lacunar patients.

Objective To explore the relationship between lacune of presumed vascular origin and spontaneous HT in elderly patients with AIS.Methods A total of 671 AIS patients hospitalized in our hospital from January 2021 to September 2024 were prospectively recruited.According to the conditions of lacune of presumed vascular origin by the first cranial MRI examination after admis-sion,they were divided into 330 cases with lacune of presumed vascular origin and 341 cases with-out,and based on the re-examination of head CT or MRI during hospitalization,they were also as-signed into a HT group(139 cases)and a non-HT group(532 cases).Multivariate logistic regres-sion analysis was conducted to clarify the correlation between lacune of presumed vascular origin and spontaneous HT in the elderly AIS patients.The incidence of spontaneous HT and the differ-ences in the subtype composition of spontaneous HT were compared between the mild and severe groups of lacune of presumed vascular origin.Results Baseline NIHSS score,atrial fibrillation,white matter lesions,enlarged perivascular spaces,cerebral microbleeds,large-area cerebral infarc-tion,and ratio of lacune of presumed vascular origin were significantly higher in the HT group than the non-HT group[(10(6,13)vs 9(5,12),P=0.000;28.1％vs 18.0％,P=0.009;74.8％vs 56.0％,P=0.000;79.9％vs 57.5％,P=0.000;30.2％vs 16.9％,P=0.000;45.3％vs 13.2％,P=0.000;66.2％vs 44.7％,P=0.000].The incidence of spontaneous HT was significantly high-er in the lacune of presumed vascular origin group than the non-lacune of presumed vascular ori-gin group(27.9％vs 13.8％,P=0.000).Multivariate logistic regression analysis indicated that lacune of presumed vascular origin was an independent risk factor for spontaneous HT after AIS(OR=2.207,95％CI:1.231-3.959).Subtype analysis on spontaneous HT showed that the patients with lacune of presumed vascular origin had the largest proportion among all subtypes than the non-lacune of presumed vascular origin patients.The incidence of spontaneous HT was significantly higher in the severe lacune of presumed vascular origin group than the mild group(38.1％vs 20.9％,P=0.001).HI2 subtype was mainly observed in the severe group with sponta-neous HT,and HI1 subtype was in the mild group with spontaneous HT.Conclusion Lacune of presumed vascular origin is an independent risk factor for spontaneous HT after AIS in elderly patients.The patients with severe lacune of presumed vascular origin are prone to the occurrence and severe spontaneous HT than the mild lacunar patients.

This research aimed to explore strategies to enhance the influence of major themed publicity in hospital party building within several prominent tertiary public hospitals in China,including one in Beijing,one in Zhejiang and four in Guang-dong.An analysis of themed publicity initiatives over the past five years,particularly during pivotal party events and key thematic activities,was undertaken.This assessment included both offline events and the online media coverage through various integrated media platforms.The study aimed to offer development suggestions for hospitals to amplify the influence of their major themed publicity strategies for Party building.Currently,hospitals are placing increased emphasis on the strategic planning of major themed publicity for Party building.Innovation in content and promotional tactics,along with strengthened integration with main-stream media,is essential.Effective dissemination that elicits empathy and presents high-quality publicity materials is crucial for achieving optimal dissemination outcomes and further elevating the hospital's Party building influence.

Humans ; Asthma/drug therapy* ; Biological Therapy

Recent advances in genome editing, especially CRISPR-Cas nucleases, have revolutionized both laboratory research and clinical therapeutics. CRISPR-Cas nucleases, together with the DNA damage repair pathway in cells, enable both genetic diversification by classical non-homologous end joining (c-NHEJ) and precise genome modification by homology-based repair (HBR). Genome editing in zygotes is a convenient way to edit the germline, paving the way for animal disease model generation, as well as human embryo genome editing therapy for some life-threatening and incurable diseases. HBR efficiency is highly dependent on the DNA donor that is utilized as a repair template. Here, we review recent progress in improving CRISPR-Cas nuclease-induced HBR in mammalian embryos by designing a suitable DNA donor. Moreover, we want to provide a guide for producing animal disease models and correcting genetic mutations through CRISPR-Cas nuclease-induced HBR in mammalian embryos. Finally, we discuss recent developments in precise genome-modification technology based on the CRISPR-Cas system.
Animals ; CRISPR-Cas Systems/genetics* ; DNA/genetics* ; Embryo, Mammalian/metabolism* ; Endonucleases/metabolism* ; Gene Editing ; Mammals/metabolism*

Although assisted reproductive technology (ART) is an effective method for infertility treatment, its safety deserves great attention. Controlled ovulation stimulation is carried out in the period when the oocyte imprinting gene completes reprogramming, and the in vitro culture of the embryo acts on the sensitive period of imprinting gene demethylation. Intracytoplasmic sperm injection (ICSI) operation will affect the genome, imprinting gene extensive erasure, imprinting reconstruction and maintenance. Gamete and embryo freezing may lead to gene expression disorder, and increase the risk of imprinting genetic diseases. Whether the non-physiological manipulation in ART process will affect the embryos epigenetic regulation program and be further inherited to the next generation needs further investigation. This article reviewed the effects of ART on the epigenetic inheritance of offspring.

Human embryos release cell-free DNA (cfDNA) into the culture medium during in vitro growth. cfDNA is composed of extracellular DNA fragments which are released from the cells as a result of necrosis or apoptosis. Use cfDNA for non-invasive preimplantation genetic testing (niPGT) has the advantages of non-invasive, easy to operate, and has made preliminary progress in the preimplantatoin genetic testing for aneuploidies and monogenic defects. However, the effectiveness of niPGT in clinical application, maternal contamination and the source of cfDNA are still controversial. In this paper, the clinical application of niPGT and the sources of cfDNA were reviewed, and the limitations and future application prospects of niPGT were put forward to provide reference for clinical practice.