Objective To investigate the clinical effect of sedation hypnotic drugs combined with sleep health education in comorbid insomnia of adult type 2 diabetes mellitus.Methods The data of general condi-tions,examination indicators,Insomnia Severity Index Scale(ISI),Self-rating Anxiety Scale(SAS),Self-rat-ing Depression Scale(SDS)scores in the patients with type 2 diabetes mellitus receiving short-term(2 weeks)insulin intensive therapy in the neurology and endocrinology department of this hospital from January to De-cember 2023 were collected.Sixty-five patients receiving the sedation hypnotic drugs combined with sleep health education served as the observation group,among them,39 cases were complicated with mild anxiety(group A1),22 cases were complicated with mild depression(group A2);fifty-four patients without receiving sedative and hypnotic drugs combined with sleep health education served as the control group,among them,33 cases were complicated with mild anxiety(group B1),22 cases were complicated with mild depression(group B2).All patients received the routine diabetes diet,exercise instruction and intensive insulin therapy.The gly-cemic control and improvement of insomnia,anxiety and depression after 1,2 weeks of treatment were com-pared among various groups.Results The reaching standard rates of fasting blood glucose(FPG),2 h post-prandial glucose(2 hPG)and time in range(TIR)in 1 week after treatment showed no statistical difference between the observation group and control group(P＞0.05);FPG and 2 hPG after 2-week treatment in the observation group were were significantly lower than those in the control group(P＜0.05),and the TIR reac-hing standard rate in the observation group was significantly higher than that in the control group(P＜0.05).The scores of ISI,SAS and SDS after 1 week treatment had no statistical difference between the obser-vation and control groups(P＞0.05);the ISI and SAS scores after 2-week treatment in the observation group were significantly lower than those in the control group(P＜0.05),The SDS score had no statistical differ-ence between the observed group and the control group(P＞0.05);the remission rate of insomnia and anxiety symptoms in 2 weeks after treatment in the observation group was significantly increased compared with that in 1 week after treatment(P＜0.05);the remission rate of insomnia,anxiety and depression symptoms in the control group had no statistic difference between in 2 weeks after treatment and 1 week after treatment(P＞0.05).Conclusion Sedation hypnotic drugs combined with sleep health education for treating comorbid in-somnia in adult patients with type 2 diabetes mellitus can not only effectively increase the reaching standard rate of blood glucose control,but also improve the symptoms of insomnia and anxiety.

Aspirin,a non-steroidal anti-inflammatory drug,is widely used in clinics with definite therapeutic effects and a few adverse drug reactions.Recent studies demonstrated that other effects besides antipyretic,analgesic,and anti-inflammatory effects have been discovered,such as anti-cancer,anti-cardiovascular diseases,anti-skeletal muscle diseases,and anti-nervous system diseases.This paper reviewed the therapeutic effects and possible mechanisms of aspirin on cancer,cardiovascular disease,muscle-skeletal disease,and nervous system disease based on the related research status at home and abroad.It will provide clues and a theoretical basis for clinical rational drug use and discover novel pharmacological effects of aspirin in the future.

Objective:To investigate the value of clinical nursing path teaching based on core competencies in the clinical teaching of urology nursing students.Methods:A total of 100 nursing students who received nursing internship in Department of Urology, China-Japan Union Hospital of Jilin University, were selected as subjects and were divided into observation group and control group using a random number table, with 50 students in each group. The students in the control group received traditional teaching, while those in the observation group received clinical nursing path teaching based on core competencies. After the end of teaching, the core competency evaluation form for nursing students was used to evaluate core competencies, and the self-learning ability evaluation tool for nursing students was used to evaluate self-learning ability. SPSS 22.0 software was used for t test. Results:Compared with the control group, the observation group had significantly higher scores of core competencies (operation skills, theoretical knowledge, self-development ability, clinical nursing ability, interpersonal communication ability, and critical thinking ability) ( P<0.05). The observation group had a significantly higher score of self-learning ability than the control group ( P<0.05). Conclusion:In the teaching of urology nursing students, the application of the clinical nursing path based on core competencies can significantly improve the core competencies of nursing interns and the quality of teaching.

C-fos is a transcription factor that plays an important role in cell proliferation, differentiation and tumor formation. The aim of this study was to clone the goat c-fos gene, clarify its biological characteristics, and further reveal its regulatory role in the differentiation of goat subcutaneous adipocytes. We cloned the c-fos gene from subcutaneous adipose tissue of Jianzhou big-eared goats by reverse transcription-polymerase chain reaction (RT-PCR) and analyzed its biological characteristics. Using real-time quantitative PCR (qPCR), we detected the expression of c-fos gene in the heart, liver, spleen, lung, kidney, subcutaneous fat, longissimus dorsi and subcutaneous adipocytes of goat upon induced differentiation for 0 h to 120 h. The goat overexpression vector pEGFP-c-fos was constructed and transfected into the subcutaneous preadipocytes for induced differentiation. The morphological changes of lipid droplet accumulation were observed by oil red O staining and bodipy staining. Furthermore, qPCR was used to test the relative mRNA level of the c-fos overexpression on adipogenic differentiation marker genes. The results showed that the cloned goat c-fos gene was 1 477 bp in length, in which the coding sequence was 1 143 bp, encoding a protein of 380 amino acids. Protein structure analysis showed that goat FOS protein has a basic leucine zipper structure, and subcellular localization prediction suggested that it was mainly distributed in the nucleus. The relative expression level of c-fos was higher in the subcutaneous adipose tissue of goats (P < 0.05), and the expression level of c-fos was significantly increased upon induced differentiation of subcutaneous preadipocyte for 48 h (P < 0.01). Overexpression of c-fos significantly inhibited the lipid droplets formation in goat subcutaneous adipocytes, significantly decreased the relative expression levels of the AP2 and C/EBPβ lipogenic marker genes (P < 0.01). Moreover, AP2 and C/EBPβ promoter are predicted to have multiple binding sites. In conclusion, the results indicated that c-fos gene was a negative regulatory factor of subcutaneous adipocyte differentiation in goats, and it might regulate the expression of AP2 and C/EBPβ gene expression.
Animals ; Goats/genetics* ; Cell Differentiation/genetics* ; Adipogenesis/genetics* ; Gene Expression Regulation ; Proteins/genetics* ; Cloning, Molecular

Objective: Sweet Tea (ST), derived from the leaves of Lithocarpus polystachyus, is a Chinese folk medicine with wide pharmacological activities. However, the promotive effects of ST water extract on hepatocytes proliferation and its underlying mechanism remains still unknown. In the present study, the beneficial effects of ST water extract on human hepatocytes and its possible mechanism were investigated. Methods: MTT assay was used to detect the safety range of ST; HL7702 cells were divided into four groups: control group, ST low- (50 μg/mL), medium- (200 μg/mL) and high-concentration (800 μg/mL) groups; BrdU ELISA and EDU staining were used to observe DNA content and cell proliferation; Moreover, flow cytometry was applied to analyze the distribution of cell cycle. Furthermore, the expression of cyclin D1, CDK4, HGF/c-Met, Akt, Erk1/2 were detected by Western blot. Results: It was found that ST water extract concentration-dependent promoted human hepatocytes HL7702 cell proliferation within 72 h through accumulating the cells in S phase and G2/M phase. Furthermore, ST water extract up-regulated expression of Cyclin D1 and CDK4 proteins. Moreover, ST water extract not only increased HGF expression and phosphorylation of c-Met level, but also activated the phosphorylation levels of AKT, ERK1/2. Interestingly, both of AKT inhibitor A6730 and ERK1/2 inhibitor U0126 reversed the promotive effects of ST water extract, which further confirmed that activation of AKT and ERK1/2 were involved. Conclusion: The findings reveal that ST water extract promoted HL7702 cells proliferation through the stimulation of cell cycle mediated by activating the AKT- and ERK1/2-related pathway.

Objective:To understand the correlation between systemic and local (at the maternal-fetal interface) oxidative stress in women with early pregnancy and the influencing factors of indicators, and to explore the associations between early pregnancy loss (EPL) and biomarkers of lipid peroxidation and total antioxidant capacity (T-AOC).Methods:A case-control study was performed. From December 2017 to July 2018, cases of EPL and controls of normal early pregnant women requesting induced abortions due to unintended pregnancies were recruited in the Family Planning Department of the Second Hospital of Tianjin Medical University. The levels of malondialdehyde (MDA) and T-AOC in serum of 103 participants and chorionic villous tissue of 60 participants in each group were measured. The influencing factors and the correlations between the levels of each biomarker in serum and villous were analyzed. The differences in the levels of each biomarker between the two groups were compared.Results:The levels of MDA and T-AOC in villi of case group and control group were both positively correlated with their serum markers, their correlation coefficients were r=0.744, P<0.001; r=0.312, P=0.015; r=0.712, P<0.001; r=0.650, P<0.001, respectively. Except for villus MDA in case group, serum and villus MDA and T-AOC increased with the increasing of gestational days in both groups. For each additional week of gestation in case group, serum MDA increased by 8.3% ( P=0.005), serum T-AOC increased by 4.2% ( P=0.002), villus T-AOC increased by 2.6% ( P=0.010), all of which were statistically significant; in control group, the serum MDA, serum T-AOC, villus MDA and villus T-AOC increased by 11.3% ( P=0.004), 5.4% ( P=0.007), 18.7% ( P=0.017) and 8.4% ( P=0.009) respectively for each additional week of gestation. After adjusting the gestational days, the serum T-AOC of the case group was 13.4% lower than that of control group ( P<0.001), the villus T-AOC was 33.7% lower than that of control group ( P<0.001), while the villus MDA was 30.1% higher than that of control group ( P=0.012). The serum MDA of the two groups had no statistic significance. Conclusion:The status of systemic oxidative stress is synchronized with that in the maternal-fetal interface in early pregnant women. Gestational age is an important factor affecting oxidative stress in serum and villous. EPL is associated with the reduction of systemic and maternal-fetal interface antioxidant capacity and maternal-fetal interface lipid peroxidation damage.

Objective:To understand the correlation between systemic and local (at the maternal-fetal interface) oxidative stress in women with early pregnancy and the influencing factors of indicators, and to explore the associations between early pregnancy loss (EPL) and biomarkers of lipid peroxidation and total antioxidant capacity (T-AOC).Methods:A case-control study was performed. From December 2017 to July 2018, cases of EPL and controls of normal early pregnant women requesting induced abortions due to unintended pregnancies were recruited in the Family Planning Department of the Second Hospital of Tianjin Medical University. The levels of malondialdehyde (MDA) and T-AOC in serum of 103 participants and chorionic villous tissue of 60 participants in each group were measured. The influencing factors and the correlations between the levels of each biomarker in serum and villous were analyzed. The differences in the levels of each biomarker between the two groups were compared.Results:The levels of MDA and T-AOC in villi of case group and control group were both positively correlated with their serum markers, their correlation coefficients were r=0.744, P<0.001; r=0.312, P=0.015; r=0.712, P<0.001; r=0.650, P<0.001, respectively. Except for villus MDA in case group, serum and villus MDA and T-AOC increased with the increasing of gestational days in both groups. For each additional week of gestation in case group, serum MDA increased by 8.3% ( P=0.005), serum T-AOC increased by 4.2% ( P=0.002), villus T-AOC increased by 2.6% ( P=0.010), all of which were statistically significant; in control group, the serum MDA, serum T-AOC, villus MDA and villus T-AOC increased by 11.3% ( P=0.004), 5.4% ( P=0.007), 18.7% ( P=0.017) and 8.4% ( P=0.009) respectively for each additional week of gestation. After adjusting the gestational days, the serum T-AOC of the case group was 13.4% lower than that of control group ( P<0.001), the villus T-AOC was 33.7% lower than that of control group ( P<0.001), while the villus MDA was 30.1% higher than that of control group ( P=0.012). The serum MDA of the two groups had no statistic significance. Conclusion:The status of systemic oxidative stress is synchronized with that in the maternal-fetal interface in early pregnant women. Gestational age is an important factor affecting oxidative stress in serum and villous. EPL is associated with the reduction of systemic and maternal-fetal interface antioxidant capacity and maternal-fetal interface lipid peroxidation damage.

[Abstract] Objective: To observe the effects of shikonin on the proliferation, apoptosis and cell cycle of human esophageal carcinoma TE-1 cells, and to explore its mechanism. Methods: TE-1 cells were treated with different concentrations of shikonin (0, 1, 5, 10 µmol/L). MTT assay was used to detect cell proliferation at different time points (24, 48 and 72 h). After treatment with shikonin for 48 h, cell apoptosis in TE-1 cells of each group was observed with Hoechst 33258 fluorescence staining. Flow cytometry was used to detect apoptosis and cell cycle. The changes in expression of TRAP1/Akt/mTOR signaling pathway related proteins were detected by Western blotting. Results: Shikonin inhibited the proliferation of TE-1 cells in a time-dose-dependent manner (P<0.05 or P<0.01). Compared with the control group, shikonin significantly promoted the apoptosis of TE-1 cells (P<0.01), induced the G0/G1 phase block of TE-1 cells (P<0.05 or P<0.01), and reduced the expression levels of TRAP1, p-Akt and p-MTOR (P<0.05 or P<0.01). The above effects were all dose-dependent. Conclusion: Shikonin can significantly inhibit the proliferation of TE-1 cells in vitro, induce G0/G1 phase arrest and promote apoptosis, which may be closely related to the inhibition of TRAP1/Akt/mTOR signaling pathway.

In recent years,the role of phosphodiesterase 5(PDE5)has been highlighted in the development and progression of neurological disease.PDE5 inhibitors show significant effect of neruoprotection,which may be related with some effects such as resistance to stroke,anti-oxidation,inhibition of neuroinflammation and amelioration of cognitive deficits.Based on the domestic and overseas researches about PDE5,this review systematically summarized the neuroprotection of PDE5 and their related mechanisms.

Objectives:To understand the current situation of premenstrual syndrome (PMS) and type D personality in female college students,and to explore the relationship between them.Methods:Totally 856 female college students were assessed with the Premenstrual Syndrome Scale (PMSS,those with total score of 6 or higher as having PMS) and Type D Personality Scale 14 (DS-14,those negative emotion and social inhibition factor with total score of 10 or higher as having type D personality).Result:A total of 280 students were detected with PMS,and the rote was 32.7％.Totally 272 students were detected with the type D personality,and the rote was 31.8％.The total score of DS-14 and the score of 2 factors were higher in PMS group than in non-PMS group (Ps ＜0.001).The rate of PMS was lower in type D personality group than in non-type D personality group (11.76％ vs.25.34％,P ＜ 0.001).Conclusions:The occurrence of premenstrual syndrome may be related to the type D personality.The evaluation of personality characteristics should be taken into consideration when formulating the intervention program.