Obstructive sleep apnea-hypopnea syndrome(OSAHS)is a chronic disease that characteristics is multi system damage,which core pathological mechanism involves a complex interaction between structural abnormalities of the upper airway and imbalances in neuromuscular regulation.With continuous advancements in imaging technique,upper airway imaging technique has been a key tool that reveals pathological characteristics of OSAHS heterogeneity,and guides individual diagnosis and treatment.This article explored OSAHS's pathogenesis,and systematically sorted out the application of various imaging techniques,including X-ray cephalometry,computed tomography(CT),magnetic resonance imaging(MRI),ultrasound,and drug-induced sleep endoscopy(DISE)in the diagnosis and treatment for OSAHS,which focuses on an analysis for their values in the location of them at obstructive plane,treatment decision and the prediction for curative effect.In addition,this article conducted a prospect for multimodal imaging fusion,artificial intelligence-assisted analysis and other development in these imaging techniques in future.By comparing the clinical applicability and limitations of each kind of technique,this review provided imaging basis for optimizing the pathway of OSAHS's clinical diagnosis and treatment,promoting the shift from conventional classification to precision intervention,so as to ultimately improve the quality of managing disease.

Purpose To explore the influence of thrombolysis therapy before percutaneous coronary intervention(PCI)on long-term left ventricular global and regional function in ST segment elevation myocardial infarction(STEMI)patients by cardiac magnetic resonance(CMR).Materials and Methods A retrospective analysis was conducted on 67 STEMI patients who were enrolled in a prospective study from November 2021 to August 2022 in the First Affiliated Hospital with Nanjing Medical University,the First People's Hospital of Lianyungang,the Affiliated Hospital of Jiangnan University,Changzhou No.2 People's Hospital and Huai'an Second People's Hospital and underwent CMR examination one year later.STEMI patients were divided into thrombolysis group and non-thrombolysis group according to whether a single half-dose of 5 mg recombinant staphylokinase(r-SAK)was given within two hours before the first medical contact and PCI.Based on CMR cine images,the traditional left ventricular function,global and segmental functional parameters were measured,and the differences between the two groups were compared.According to the degree of late gadolinium enhancement involvement,myocardial segments were divided into the following four types:transmural infarcted segments,non-transmural infarcted segments,locally infarcted segments,and non-infarcted segments.The parameters of the two groups were compared across these different segments.Results At the patient level,the cardiac index,left ventricular wall thickening and left ventricular wall motion in r-SAK thrombolysis group were higher than those in non-thrombolysis group(t/Z=-2.426,-4.307,-2.735,all P<0.05).At the segment level,compared with non-thrombolysis group,patients received r-SAK before primary PCI showed greater segmental radial strain in non-transmural infarcted segments(Z=-2.117,P=0.034);larger segmental wall motion in locally infarcted segments(Z=-2.235,P=0.025),and better segmental circumference strain in the non-infarcted segments(Z=-3.869,P<0.001).Conclusion The thrombolytic therapy before PCI in STEMI patients retain better long-term left ventricular global and regional function evaluating by CMR.

Purpose To explore the influence of thrombolysis therapy before percutaneous coronary intervention(PCI)on long-term left ventricular global and regional function in ST segment elevation myocardial infarction(STEMI)patients by cardiac magnetic resonance(CMR).Materials and Methods A retrospective analysis was conducted on 67 STEMI patients who were enrolled in a prospective study from November 2021 to August 2022 in the First Affiliated Hospital with Nanjing Medical University,the First People's Hospital of Lianyungang,the Affiliated Hospital of Jiangnan University,Changzhou No.2 People's Hospital and Huai'an Second People's Hospital and underwent CMR examination one year later.STEMI patients were divided into thrombolysis group and non-thrombolysis group according to whether a single half-dose of 5 mg recombinant staphylokinase(r-SAK)was given within two hours before the first medical contact and PCI.Based on CMR cine images,the traditional left ventricular function,global and segmental functional parameters were measured,and the differences between the two groups were compared.According to the degree of late gadolinium enhancement involvement,myocardial segments were divided into the following four types:transmural infarcted segments,non-transmural infarcted segments,locally infarcted segments,and non-infarcted segments.The parameters of the two groups were compared across these different segments.Results At the patient level,the cardiac index,left ventricular wall thickening and left ventricular wall motion in r-SAK thrombolysis group were higher than those in non-thrombolysis group(t/Z=-2.426,-4.307,-2.735,all P<0.05).At the segment level,compared with non-thrombolysis group,patients received r-SAK before primary PCI showed greater segmental radial strain in non-transmural infarcted segments(Z=-2.117,P=0.034);larger segmental wall motion in locally infarcted segments(Z=-2.235,P=0.025),and better segmental circumference strain in the non-infarcted segments(Z=-3.869,P<0.001).Conclusion The thrombolytic therapy before PCI in STEMI patients retain better long-term left ventricular global and regional function evaluating by CMR.

Obstructive sleep apnea-hypopnea syndrome(OSAHS)is a chronic disease that characteristics is multi system damage,which core pathological mechanism involves a complex interaction between structural abnormalities of the upper airway and imbalances in neuromuscular regulation.With continuous advancements in imaging technique,upper airway imaging technique has been a key tool that reveals pathological characteristics of OSAHS heterogeneity,and guides individual diagnosis and treatment.This article explored OSAHS's pathogenesis,and systematically sorted out the application of various imaging techniques,including X-ray cephalometry,computed tomography(CT),magnetic resonance imaging(MRI),ultrasound,and drug-induced sleep endoscopy(DISE)in the diagnosis and treatment for OSAHS,which focuses on an analysis for their values in the location of them at obstructive plane,treatment decision and the prediction for curative effect.In addition,this article conducted a prospect for multimodal imaging fusion,artificial intelligence-assisted analysis and other development in these imaging techniques in future.By comparing the clinical applicability and limitations of each kind of technique,this review provided imaging basis for optimizing the pathway of OSAHS's clinical diagnosis and treatment,promoting the shift from conventional classification to precision intervention,so as to ultimately improve the quality of managing disease.

Objective:To investigate the effect of polystyrene microplastics(PS-MPs)combined with high-fat treatment on vascular endothelial cells.Methods:Human umbilical vein endothelial cells(HUVECs)were cultured in the DMEM medium containing 5%fe-tal bovine serum.HUVECs were treated with conventional culture,high-fat treatment,and PS-MPs combined with high-fat treatment.The experiment was conducted in the three groups of control group,high-fat treatment group and PS-MPs+high-fat treatment group.CCK-8 assay was used to measure cell viability,F-actin staining was used to observe cell morphological changes,and flow cytometry,scratch assay,and tube formation assay were used to measure the apoptosis,migration,and tube-forming ability of cells.Results:After HUVECs were exposed to the high-fat environment,there was a significant reduction in cell viability,shrinkage of cells,a signifi-cant increase in cell apoptosis,and significant reductions in cell migration and tube-forming ability.Compared with the high-fat treat-ment group,there were no significant changes in cell viability,cell morphology,cell apoptosis,and cell migration ability after PS-MPs combined with high-fat treatment,but the tube-forming ability of cells was further impaired.Conclusion:High-fat treatment will affect cell viability,change cell morphology,and damage vascular endothelial cell function,and PS-MPs combined with high-fat treat-ment can aggravate the damage of vascular endothelial cell function.

Objective To identify potential biomarkers and establish a prediction model for chemotherapy-related hepatotoxicity susceptibility based on plasma endogenous metabolites of colorectal cancer(CRC)patients before chemotherapy.Methods The plasma samples of 50 CRC patients before capecitabine chemotherapy and the records of their chemotherapy-related hepatotoxicity during the follow-up were collected.An ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry(UHPLC-Q-TOF-MS)was used to perform untargeted metabolomic analysis.Based on bioinformatics analysis,differential analysis,correlation analysis,and random forest were used to screen for hepatotoxicity-related plasma endogenous metabolites.All samples were randomly assigned(7∶3)to training set or test set.A multivariate logistic regression model was established to predict the hepatotoxicity of capecitabine chemotherapy based on the training set data.The prediction effects of the model in the training,test and entire sets were evaluated by receiver operating characteristic(ROC)curve analysis.Results The endogenous metabolites related to hepatotoxicity in the plasma of CRC patients before chemotherapy were mainly lipid endogenous metabolites.A series of potentially important predictive biomarkers for hepatotoxicity susceptibility were identified,including sphingamine-1-phosphate,ceramide,galactose,arachidonic acid,tyrosine,biliverdin,myristic acid,phosphatidylcholine(35∶1),phosphatidylethanolamine(36∶1),and hexadecanoic acid.The area under curve values of the prediction model based on the above biomarkers in the training,test and entire sets were 0.946(95%confidence interval[CI]0.842-1.000),0.920(95%CI 0.720-1.000),and 0.912(95%CI 0.810-0.982),respectively.Conclusion The endogenous metabolites in the plasma of CRC patients before chemotherapy can effectively predict the hepatotoxicity of capecitabine chemotherapy.These hepatotoxicity biomarkers indicate that susceptible patients have characteristics related to lipid metabolism disorders.

Self-aid and buddy-aid are primary steps in battlefield first aid,offering the timeliest treatment effectiveness.Medications are indispensable key supplies in battlefield first aid,playing a crucial role in the timely treatment of the injured.This review provides an overview of medication preparation for battlefield first aid both domestically and internationally,aiming to provide reference for the medication preparation for self-aid and buddy-aid of naval combat injuries,so as to enhance the Navy's first-aid capability and medical support capability.

This study aims to optimize the process for preparing chitosan-based ultrasound-coupled hydrogel pads and investigate their application potential in ultrasonography. Chitosan, 2-acrylamido-2-methylpropanesulfonic acid, and N-isopropylacrylamide were used as the main materials to prepare chitosan-based ultrasound-coupled hydrogel pads. The free-radical polymerization conditions were optimized by a three-factor, three-level orthogonal test with the tensile strength and ultrasound image quality of the hydrogel pads as evaluation indicators. The optimal prescription was selected by optimizing three factors of raw material ratio, polymerization temperature, and freeze-drying time. The structure and performance of the hydrogel pads were characterized by a scanning electron microscope, a universal testing machine, and an ultrasonic diagnostic instrument. The results showed that the optimal prescription was as follows: the chitosan: 2-acrylamide-2-methylpropanesulfonic acid: N-isopropylacrylamide ratio of 2:0.55:17.27, the polymerization temperature of 25 ℃, and the freeze-drying time of 48 h. The ultrasonically-coupled hydrogel pads prepared under these conditions were transparent, with a porous structure, good adhesion, and high tensile strength. The hydrogel pads had good swelling properties and the swelling degree decreased slowly on day 10. The quality of the ultrasound images obtained via chitosan-based hydrogel pads was not significantly different from that obtained via medical ultrasound coupling agent. In this study, we analyzed the effects of different preparation processes on the gel formation of chitosan-based ultrasound-coupled hydrogel pads. The hydrogel pads were transparent and mild and non-irritating to the human body, serving as an ultrasound transmission material for ultrasonography.
Chitosan/chemistry* ; Hydrogels/chemistry* ; Acrylamides/chemistry* ; Ultrasonography ; Polymerization ; Sulfonic Acids/chemistry* ; Alkanesulfonates/chemistry* ; Tensile Strength ; Freeze Drying ; Temperature

Atrial fibrillation(AF)is the most common cardiac arrhythmia.Many medical conditions,including hypertension,diabetes,obesity,sleep apnea,and heart failure(HF),increase the risk for AF.Car-diomyocytes have unique metabolic characteristics to maintain adenosine triphosphate production.Significant changes occur in myocardial metabolism in AF.Glucagon-like peptide-1 receptor agonists(GLP-1 RAs)have been used to control blood glucose fluctuations and weight in the treatment of type 2 diabetes mellitus(T2DM)and obesity.GLP-1 RAs have also been shown to reduce oxidative stress,inflammation,autonomic nervous system modulation,and mitochondrial function.This article reviews the changes in metabolic characteristics in cardiomyocytes in AF.Although the clinical trial outcomes are unsatisfactory,the findings demonstrate that GLP-1 RAs can improve myocardial metabolism in the presence of various risk factors,lowering the incidence of AF.

Objective To analyze the correlation between gasdermin E(GSDME)and the occurrence and development of cervical cancer.Methods HPA database and GEPIA2 online website were used to analyze the differential expression of GSDME gene and protein between cervical cancer tissues and normal cervical tissues.GEPIA2 and KM plotter online website was used to analyze the correlation be-tween GSDME gene expression and the pathological staging,survival and prognosis of cervical cancer.cBioPortal online website was used to analyze the GSDME gene mutation in cervical cancer tissues.TIMER2 online website and R-package were used to analyze the correla-tion between GSDME and immune infiltration in cervical cancer tissues.Gene set enrichment analysis was used to analyze the biological function of GSDME in development of cervical cancer.Real-time quantitative polymerase chain reaction(RT-qPCR)and Western blot were used to verify the transcription and translation of GSDME in cervical cancer cell lines.Results Compared with normal cervical tis-sues,the expression of GSDME mRNA and protein in cervical cancer tissues was lower(P＜0.05).Survival analysis showed that the high expression of GSDME gene may be associated with poor prognosis of cervical cancer patients.0.36％of GSDME amplification muta-tion was found in cervical squamous cell carcinoma.There was significant negative correlation between high expression of GSDME gene and B lymphocyte infiltration,whereas a significant positive correlation between high GSDME gene and mast cell infiltration in cervical cancer tissues(P＜0.05).The high expression of GSDME in cervical cancer exhibited a significant negative correlation with glutathione peroxidase activity,mRNA modification,keratin filament,and other biological functions(P＜0.001).RT-qPCR and Western blot con-firmed that the transcription and protein expression of GSDME could be detected in both human papilloma virus(HPV)positive and nega-tive cervical cancer cells.Conclusion GSDME may be a risk factor related to cervical cancer,and the correlation analysis between GS-DME and cervical cancer provides a new perspective for the follow-up targeted treatment of cervical cancer based on GSDME.