Objective: To investigate the status of latent tuberculosis infection（LTBI）among freshmen in middle schools and their willingness of preventive treatment in Yunyan District of Guiyang, so as to provide a reference for the prevention and control of tuberculosis in schools. Methods: The tuberculin skin test (TST) and X-ray scans were used to screen the TB infection of 13 915 freshmen in middle schools in Yunyan District of Guiyang in 2023, and a questionnaire survey on the willingness of accepting tuberculosis preventive treatment was conducted to LTBI patients. The χ 2 test and Fisher exact probability was conducted for the comparison of the rates among the groups. Results: Among the freshmen screened, the detection rate of LTBI was 3.29%. There were statistically significant difference in LTBI rates among freshmen of different genders (boys:2.87%, girls:3.81%), age groups (12-15 years old:3.31%, 16-17 years old:3.92%, 18-20 years old:1.91%), and school stages (junior high school:3.52%, ordinary high school:5.96%, vocational high school:2.29%)（ χ 2=9.59, 13.08, 54.30, P ＜0.01). A total of 356 LTBI freshmen completed questionnaire survey,and 299(83.99%) were willing to accept tuberculosis preventive treatment, but the actual number of LIBI freshman who underwent preventive treatment was zero. Those LTBI who had received Bacille Calmette Guérin(BCG) vaccine(86.97%) was higher in the reporting rates of being willing to accept preventive treatment than that of LTBI who had not received BCG vaccine( 75.79 %),the differences were statistically significant( χ 2=6.48， P <0.05). The main reasons for refusing preventive treatment was worry about adverse drug reactions(80.70%), social acceptance and the support of social institutions were needed most(85.96%). Conclusions The LTBI rate among freshmen in Yunyan District of GuiYang is higher. Although the freshmen with LTBI have a higher willingness to accept preventive treatment, however, no one has undergone preventive treatment. Corresponding measures need to be taken for improving the preventive treatment rate of LIBI freshmen.

Objective: To investigate the relationships among psychological resilience, parental control, and behavioral problems in middle school students, aiming to provide evidencebased recommendations for the prevention and intervention of behavioral issues in middle school students, as well as for the development of related policies. Methods: A stratified cluster sampling method was used to select 2 745 participants from three cities in Guizhou Province with different levels of economic development from August to December 2021. The Child Behavior Checklist (CBCL), Parental Control Questionnaire, and Psychological Resilience Questionnaire were administered to junior high school students and their parents. A network analysis method was employed to construct a network analysis model of factors influencing behavioral problems. Results: The detection rate of behavioral problems among junior high school students in Guizhou Province was 22.62%. The scores for psychological resilience (goal focus, emotional control, positive cognition, family support, and interpersonal assistance) and proactive inquiry in behavior control were higher in the group without behavioral problems than in the group with behavioral problems. Scores for psychological control (inducing guilt, with drawing affection, and asserting authority) were higher in the group with behavioral problems than in the group without behavioral problems, with statistically significant differences (t=9.80, 17.76, 6.21, 12.20, 13.18, 6.28, 11.58, 11.10, 10.74, P<0.05). The network model showed that among the same variable factors, the strongest connection weight was between inducing guilt and withdrawing affection, with a weight of 0.79. Between different variables, there were negative correlation between behavioral problems and psychological resilience (goal focus, emotional control, positive cognition, family support, interpersonal assistance) and behavior control (proactive inquiry, behavioral restraint) with correlation coefficients (r=-0.25, -0.42, -0.16, -0.31, -0.33, -0.17, -0.03, P<0.05), respectively. There were positive correlation between psychological control factors (inducing guilt, withdrawing affection, and asserting authority) and behavioral problems (r=0.29, 0.27, 0.27), and a negative correlation between these psychological control factors and psychological resilience factors (goal focus, emotional control, positive cognition, family support, interpersonal assistance)(r=-0.53--0.13)(P<0.05). The strongest connection weight was between withdrawing affection and family support, with a connection weight of -0.53. Family support was an important bridge symptom connecting the entire behavioral problem network model, with a high centrality. Conclusions The detection rate of behavioral problems among junior high school students in Guizhou Province is relatively high. Assisting adolescents in establishing a supportive family environment facilitates the cultivation of their psychological resilience, thereby mitigating the occurrence of behavioral problems.

Objective:The epidemiological and host animal pathogen data of leptospirosis in the population of Zhejiang Province in 2023 were analyzed, providing scientific basis for formulating prevention and control strategies of leptospirosis.Methods:The data of human leptospirosis in the population were collected from the China Information System for Disease Control and Prevention, and analyzed using descriptive epidemiological methods. The data on isolation, culture, and nucleic acid testing of Leptospira pathogens from mouse kidneys, frog kidneys, pig kidneys, and duck kidneys as well as duck serum antibody data were collected from Zhejiang Provincial Center for Disease Control and Prevention "Leptospirosis Surveillance Project of Zhejiang Province". The carrying and changing status of Leptospira epidemic microbiota in populations and host animals were analyzed. Results:In 2023, a total of 83 cases of leptospirosis were reported in Zhejiang Province, with a incidence rate of 0.126 2/ 100 000, aged (62.66 ± 11.31) years, including 68 males and 15 females. Leptospirosis cases were reported in 11 cities, mainly concentrated in the southern cities of Wenzhou City, Lishui City and Taizhou City(a total of 68 cases), accounting for 81.93% of the total number of cases. August to October were high incidence months for leptospirosis (a total of 70 cases), accounting for 84.34% of the total number cases. The male to female ratio of patients was 4.53 ∶ 1.00, and all were adults ≥20 years old, the middle and old people aged 45 - 79 years were the high-risk population (a total of 77 cases), accounting for 92.77% of the total number of cases. The patient's occupation was mainly farmers, with a total of 54 cases, accounting for 65.06% of the total number cases. The shortest time from onset to initial diagnosis for patients with leptospirosis was 0 day, and the longest was 13 days. The shortest time from initial diagnosis to confirmed diagnosis was 0 day, and the longest was 16 days. The 72.29% of the leptospirosis cases (60 cases) had a history of field labor or suspected contact with contaminated water within one month before the onset of the disease, and 18.07% of the leptospirosis cases (15 cases) had a history of contact with animals such as mice, frogs, pigs, cows, dogs, fish or ducks, or their excreta within one month before the onset of the disease. The average nucleic acid positive rate of host animals with leptospirosis was 5.92% (31/524) in mouse kidney, 6.74% (36/534) in frog kidney, and 0.66% (1/151) in pig kidney. The isolation and culture of leptospirosis from duck kidney, nucleic acid detection, and antibody detection in duck blood were all negative. The leptospirosis bacteria detected in human population were serogroup Icterohaemorrhagiae (3 samples) and Hebdomadis (4 samples), and the bacteria group detected in host animals was serogroup Icterohaemorrhagiae (3 samples). Conclusions:The outbreak of leptospirosis in Zhejiang Province mainly occurs in the summer and autumn, with the affected areas mainly concentrated in the southern region of Zhejiang Province. The affected population is mainly middle-aged and elderly males, and the population carrying Leptospira is still mainly composed of the serogroup Icterohaemorrhagiae and the Hebdomadis, with the host animal being the serogroup Icterohaemorrhagiae.

Objective: To explore the current status related to behavioral problems among children and adolescents aged 12-16 years old in Guizhou Province, China, and to provide a reference for timely prevention and intervention measures. Methods: To capture different economic development levels, a multistage stratified cluster sampling method was used. A total of 3 217 children and adolescents aged 12-16 years old from six middle schools and six primary schools in Guiyang, Duyun, Kaili City from Guizhou Province participated in this study. The Achenbach Child Behavior Checklist (CBCL) and a self designed questionnaire were administered between August and December 2021. Results: The overall detection rate of behavioral problems among the students was 16.60% , and the rate was 14.41% and 18.62% among male and female students, respectively. The results of the Logistic regression analysis showed that good peer relationships ( OR=0.25, 0.16, P <0.05) were associated with a lower risk of behavioral problems in children and adolescents. However, the following factors were associated with an elevated risk of behavioral problems: nonparents as the main caregivers( OR = 1.44 ); children s daily screen time was 1-2 h ( OR =1.47) or more than 2 h( OR =2.75); children who had no sports hobbies( OR = 1.36); father parenting for doting or neglect( OR =1.85, 2.01); maternal parenting for doting( OR = 2.12 ); father had bad life habits( OR =1.69); father and mother were occasionally sick( OR =1.30, 1.38)( P <0.05). Conclusion Behavioral problems among children and adolescents in Guizhou Province is related to children s unhealthy living habits and various factors in the family environment. It is suggested that families, schools and relevant functional departments give full play to their own advantages and take comprehensive measures to prevent the occurrence of behavioral problems among children and adolescents.

Objective: To understand the sanitation status of rural primary and secondary schools during 2015-2019, and provide data support for the formulation of environmental sanitation policies for schools in rural areas of Guiyang. Methods: According to the requirements of the Work Plan for the Rural Environmental Sanitation Monitoring Project in Guiyang City from 2015-2019, 145 rural schools in Guiyang were selected by stratified random sampling method, including 75 elementary schools and 70 junior high schools. Through on site inspection and questionnaire survey, information regarding school basic situation, drinking water for students, water supply and toilet sanitation in school. Results: Students drinking water was mainly bottled water or water from a water purifier, the proportional rate increased from 37.9% to 72.4%; water supply in the school was mainly from village water plant, from 69.0% to 93.1%; the proportion of schools with independent public toilets and toilets in dormitory buildings ranged from 65.6% to 100.0%, the proportion of schools with toilets in teaching buildings was less than 60.0%, but the penetration rate of sanitary toilets in independent public toilets, teaching buildings and dormitory buildings were more than 80.0%. Qualified rate of squatting in the men s toilet fluctuated between 93.1% and 100.0%. Qualified rate of the squatting in the women s toilet had increased from 44.8% to 65.5%. Proportion of school toilets guarded by specific staff decreased from 31.0% to 27.6%. The proportion of soap increased from 20.7% to 58.6%, the difference was statistically significant ( χ 2=21.27, P =0.01). Conclusion During 2015-2019, the environmental sanitation in rural areas of Guiyang was acceptable, with students drinking water, school water supply and sanitary toilets moderately improved. Toilets in the teaching building and women s toilet squatting numbers need to be increased. School toilets managed by specific personnel, as well as good hygiene habits education promotion are encouraged.

Objective: To explore the characteristics of psychological control of parents of college students and their correlation with health risk behaviors, and to provide a reference for further improving parenting styles and improving the physical and mental health of college students. Methods: With the method of multi-stage stratified random sampling, 3 318 college students from 6 universities in Guizhou Province were selected. The parents’ psychological control questionnaire and Chinese adolescent health-related behavior questionnaire were used to conduct anonymous field survey. Results: A total of 660 students’ parents have got low score of mental control in low-score group (<26), 1 844 in the middle group (26-49) and 814 in the high group (>49). Students who had suicide plans scored higher in the dimensions of invasion of privacy, inhibition of speech expression, and sense of disability than those who had not(t=4.08, 3.74, 3.82, P<0.01); The scores of smokers in achievement expectation, invasion of privacy and ability ineffectiveness were higher than those of non-smokers(t=7.12, 3.91, 8.10, P<0.01); the scores of drinkers in all dimensions were higher than those of non drinkers(t=9.35, 4.64, 5.90, 8.78, P<0.01); the scores of Internet addicts in all dimensions were higher than those of non-smokers(t=8.92, 14.27, 3.70, 15.94, P<0.01). Spearman correlation analysis showed that smoking, drinking and Internet addiction were positively correlated with parents’ psychological control(r=0.11, 0.15, 0.19, P<0.01). Conclusion Parents’ psychological control of college students in achievement expectation, ability ineffectiveness and speech inhibition; parents’ psychological control is positively related to smoking, drinking and Internet addiction behavior. Parents should give more active encouragement and guidance to their children and strengthen communication.

Objective: To analyze the prevalence and associated factors of suicide ideation among adolescent students in Guizhou Province, and to provide scientific evidence for developing strategy. Methods: Multistage stratified cluster sampling method was used to recruit 4 903 adolescents from 14 middle schools in Guizhou Province. China Youth Health Risk Behaviors Questionnaire was used and data were analyzed by using univariate analysis and Logistic regression analysis. Results: Totally 14.3%(703/4 903) middle school students had suicide ideation during the past 12month period, in which 13.2% were boys and 15.4% were girls, more girls were prone to suicide than boys(χ2=5.20, P<0.05); 13.7% junior students, 15.0% senior students, 14.5% Han ethnic students and 14.1% minority students suicide ideation, and the differences between grades and ethnic groups were of no statistical significance (P>0.05). The multivariates Logistic regression analysis showed that risk factors for suicidal ideation included being girl(OR=1.49),higher maternal education(college and above: OR=2.26), suffering psychological bullying over the past 30 days(OR=1.30), taking part in fighting for the past 12 months (OR=1.33), often feeling lonely(OR=1.86), sleeplessness(OR=1.38), deliberate selfinjury(OR=3.58),decreases of daily activities due to depression(OR=1.87), ideation of running away(OR=3.31), internet addiction(OR=1.37), walk violating regulations(OR=2.42), but occasional low mood from academic pressure(OR=0.73) was a protective factor for suicidal ideation(P<0.05). Conclusion Suicide ideation of middle school students is closely related to campus violence, adverse inner metal experience and unhealthy behavior pattern. All sectors of society should attach great importance and targeted measures to prevent suicide and protect the students.

Objective: To understand the smoking of college students in Guizhou Province and to explore associated factors for better prevention and control of smoking behavior. Methods: A multi-stage stratified random sampling method was used to describe the current smoking situation of college students. And 2 451 college students of different majors and grades from three cities with high, medium and low economic development levels were selected. Besides, we used multivariate logistic regression analysis to analyze the major factors affecting current smoking. Results: Ever smoking rate of college students in Guizhou Province was 50.8%, the peak age of first smoking attempt was 8-13(35.2%); current smoking rate of college students in Guizhou Province was 20.8%. Logistic regression analysis showed that male, upper grade, monthly living expenses of not less than 1 000 yuan, single parents, family smoking and peer smoking were the main risk factors affecting college students’ smoking(OR=5.52,1.44,1.48,1.69,1.47,1.88,P<0.05). Conclusion College students in Guizhou Province have a higher rate of smoking attempt and low rate of current smoking, as well as low tobacco dependence which is determined by multiple factors. Tobacco hazard education, mental health education and living environment intervention for college students may help reduce the probabitity for tobacco use.

Objective To analysis the rationality of atorvastatin calcium and simvastatin in outpatient and emergency departments from the perspective of drug interactions. Methods All prescrip-tions comprising atorvastatin calcium and simvastatin in the outpatient and emergency departments in Shaanxi Provincial People′s Hospital from July 1 to December 31,2017 were collected. The potential adverse drug interactions in these prescriptions were analyzed and the rationality of drug uses was determined by referring to the drug labels and relevant domestic and foreign guidelines. Results A total of 3 647 prescriptions in the outpatient and emergency departments were collected,of which 2 730 (74.9%)comprised atorvastatin calcium and 917 (25. 1%)comprised simvastatin. Among the 3 647 prescriptions,3 221 (88. 3%) comprised more than one drug,of which 2 432 (75.5%)comprised atorvastatin calcium and 789 (24.5%) comprised simvastatin. In the 3 221 co-prescriptions comprising statins,878 (27.3%)comprised other substrates of cytochrome P450 (CYP)3A4,mainly including adenosine diphosphate receptor inhibitors, calcium channel blockers,and proton pump inhibitors,of which clopidogrel and nifedipine were used more frequently;311 (9.7%)comprised CYP 3A4 inhibitors,mainly including calcium channel blockers,anti-arrhythmia agents,immunosuppressive agents,and antibacterial drugs,of which amlodipine and diltiazem were used more frequently;17 (0. 5%) comprised CYP3A4 inductions,including pioglitazone and carbamazepine. Among the co-prescriptions of statins and CYP 3A4 inhibitors,6 prescriptions comprised incompatibility drugs (simvastatin combined with clarithromycin)and 15 prescriptions comprised simvastatin or atorvastatin calcium with dose higher than the recommended. Conclusion Drug combination of atorvastatin calcium or simvastatin in some prescriptions of Shaanxi Provincial People′s Hospital is unreasonable,mainly shown as being combined with drugs having potential adverse interactions or overdosage when combined with CYP 3A4 inhibitors.

Objective To analysis the rationality of atorvastatin calcium and simvastatin in outpatient and emergency departments from the perspective of drug interactions. Methods All prescrip-tions comprising atorvastatin calcium and simvastatin in the outpatient and emergency departments in Shaanxi Provincial People′s Hospital from July 1 to December 31,2017 were collected. The potential adverse drug interactions in these prescriptions were analyzed and the rationality of drug uses was determined by referring to the drug labels and relevant domestic and foreign guidelines. Results A total of 3 647 prescriptions in the outpatient and emergency departments were collected,of which 2 730 (74.9%)comprised atorvastatin calcium and 917 (25. 1%)comprised simvastatin. Among the 3 647 prescriptions,3 221 (88. 3%) comprised more than one drug,of which 2 432 (75.5%)comprised atorvastatin calcium and 789 (24.5%) comprised simvastatin. In the 3 221 co-prescriptions comprising statins,878 (27.3%)comprised other substrates of cytochrome P450 (CYP)3A4,mainly including adenosine diphosphate receptor inhibitors, calcium channel blockers,and proton pump inhibitors,of which clopidogrel and nifedipine were used more frequently;311 (9.7%)comprised CYP 3A4 inhibitors,mainly including calcium channel blockers,anti-arrhythmia agents,immunosuppressive agents,and antibacterial drugs,of which amlodipine and diltiazem were used more frequently;17 (0. 5%) comprised CYP3A4 inductions,including pioglitazone and carbamazepine. Among the co-prescriptions of statins and CYP 3A4 inhibitors,6 prescriptions comprised incompatibility drugs (simvastatin combined with clarithromycin)and 15 prescriptions comprised simvastatin or atorvastatin calcium with dose higher than the recommended. Conclusion Drug combination of atorvastatin calcium or simvastatin in some prescriptions of Shaanxi Provincial People′s Hospital is unreasonable,mainly shown as being combined with drugs having potential adverse interactions or overdosage when combined with CYP 3A4 inhibitors.