Objective:To investigate relationship between expression of ADGRG5 and clinical prognosis and immune response in pancreatic adenocarcinoma(PAAD).Methods:ADGRG5 expression in PAAD and normal tissues were compared by Wilcoxon rank sum test.Diagnostic value of ADGRG5 was evaluated by ROC curve in PAAD.Kaplan-Meier method and Cox regres-sion analysis were used to evaluate prognostic factors.Gene set enrichment analysis(GSEA)and immune infiltration analysis were applied to annotate biological function of ADGRG5.Results:ADGRG5 expression in PAAD was significantly higher than normal tissue(P=2.8e-32).ADGRG5 had significant diagnostic and prognostic ability for PAAD(AUC=0.866).High ADGRG5 expression predicted a good progress free interval(PFI)(P=0.01),and expression of ADGRG5 was independently associated with PFI(HR:0.656,95%CI:0.433～0.972,P=0.035).ADGRG5 expression was related to regulation of immunomodulatory pathway and function of some types of immune infiltrating cells.Conclusion:Increased ADGRG5 may be a potential biomarker for PAAD diagnosis and prognosis,which affects prognosis of PAAD patients and significantly correlated with immune infiltration.

Objective:To investigate relationship between expression of ADGRG5 and clinical prognosis and immune response in pancreatic adenocarcinoma(PAAD).Methods:ADGRG5 expression in PAAD and normal tissues were compared by Wilcoxon rank sum test.Diagnostic value of ADGRG5 was evaluated by ROC curve in PAAD.Kaplan-Meier method and Cox regres-sion analysis were used to evaluate prognostic factors.Gene set enrichment analysis(GSEA)and immune infiltration analysis were applied to annotate biological function of ADGRG5.Results:ADGRG5 expression in PAAD was significantly higher than normal tissue(P=2.8e-32).ADGRG5 had significant diagnostic and prognostic ability for PAAD(AUC=0.866).High ADGRG5 expression predicted a good progress free interval(PFI)(P=0.01),and expression of ADGRG5 was independently associated with PFI(HR:0.656,95%CI:0.433～0.972,P=0.035).ADGRG5 expression was related to regulation of immunomodulatory pathway and function of some types of immune infiltrating cells.Conclusion:Increased ADGRG5 may be a potential biomarker for PAAD diagnosis and prognosis,which affects prognosis of PAAD patients and significantly correlated with immune infiltration.

Objective To explore the stress among patients in Emergency Observation Room and its correlation with coping modes.Methods A total of 300 patients in Emergency Observation Room of a Class Ⅲ grade A hospital were recruited by convenient sampling method for a cross-sectional survey and its correlation, from September 2014 to September 2015, using the general situation questionnaire, stress-reaction questionnaire (SRQ) and medical coping modes questionnaire (MCMQ). The data of survey was compared with norm, and Pearson correlation analysis was used to analyze the correlation between stress reaction and medical response level.Results The score of stress reaction was (56.25±20.20), in which somatic reaction was (19.41±7.87), higher than norm of hospitalized patients and the behavior reaction was (10.65±4.82),lower than the norm of hospitalized patients (t=6.034, -9.143;P<0.01). The total score of coping style of patients in emergency observation room was (39.26±6.97), in which the face dimension was (17.63±3.90), avoidance (13.96±3.28), yield (7.66±2.66), all lower than the norm of hospitalized patients (t=-8.200,-2.514,-7.485;P<0.05). The correlation was positive between stress reaction and coping styles (r=0.125-0.510,P<0.05). Conclusions The stress has close correlation with coping style for patients in Emergency Observation Room. Medical personnel should guide emergency patients to correctly face stress, and improve their coping styles.

Objective To explore the current status of stress level and its influencing factors among patients in observation room of emergency department .Methods Totally 300 patients in the observation room of emergency department from September 2014 to September 2015 were recruited in this study by convenience sample method.General situation questionnaire and stress-reacton questionnaire ( SRQ) were used to conduct this cross-sectional survey .Results The scores of SRQ and body reaction factor were ( 56.25 ±20.20) and (19.41±7.87), and they were all significantly higher than the national norm (t=2.231, 6.034;P<0.05).The score of behavior reaction was (10.65±4.82), and it was lower than the national norm (t=-9.143, P<0.01). There was no significant difference between the score of emotional reaction and national norm ( P>0.05) .Single-factor analysis showed that , the differences in the score of emotional reaction among patients with different working situation and monthly income were significant (F=3.684,4.109,P<0.05).The differences in the score of body reaction among patients with different working situation , occupation , monthly income and religion were significant (F=4.851,3.624,2.678,5.033;P<0.05).The differences in the score of behavior reaction among patients with different working situation and occupation were significant (F=4.181,2.489;P<0.05).Multiple stepwise regression analysis showed that religious faith and monthly income were identified as significant influencing factors of stress level (P<0.05).Conclusions Medical personnel should guide patients to correctly face the stress based on their religious faith and monthly income , so as to promote the recovery of health .

Objective To observe selective cyclooxygenase-2 (COX-2) inhibitor celecoxib on the cell migration, MMP-9 and VEGF expression of human gastric cancer SGC-7901. Methods In vitro culture of SGC-7901 cells, the cells were divided into blank control group and celecoxib treatment group (different concentrations of celecoxib 25, 50 and 100μmol/L). SGC-7901 cells were treated with celecoxib for 24 h. The effect of celecoxib on cell migration of SGC-7901 was detected by the damage repair experiment. RT-PCR assay was used to detect the effect of celecoxib on MMP-9 mRNA and VEGF mRNA expressions in SGC-7901 cells. Results The migration distance of SGC-7901 cells was decreased with the increased concentration of celecoxib. There were significant differences between medium-dose and high-dose celecoxib groups and the control group. RT-PCR assay showed that MMP-9 mRNA and VEGF mRNA expressions in gastric cancer SGC-7901 were significantly decreased with the increased concentration of celecoxib, and there was a significant difference compared with those of control group (P＜0.05). Conclusion Celecoxib can inhibit migration of human gastric cancer SGC-7901 cells, which may be related to the inhibition of COX-2 downstream of MMP-9 and VEGF expressions.

The goal of hospital information system construction is to establish a comprehensive,safe and stable information network.A new or relocated hospital is redesigned and has overall layout,which facilitates achieving the objective of information system construction.The authors introduce the architecture and construction of information network in the process of hospitaI relocation.The important points of information coverage at different functional areas are determined,including the key business area,non-key business area,server area,radiological area,and other functional areas,which may improve the effectiveness of the investment.At the same time,the possibility of large area information system paralysis due to network fault is minimized to ensure the security of information system.