Objective:To explore the relationship between coronary artery measurements combined with C-reactive protein(CRP), erythrocyte sedimentation rate(ESR) and coronary artery lesionsin(CAL) with incomplete Kawasaki disease (IKD) in children.Methods:Sixty-eight cases of pediatric IKD admitted to Huaibei Maternal and Child Health Care Hospital from January 2020 to April 2024 were selected as the IKD group, and 34 healthy children undergoing physical examination during the same period were selected as the control group according to the principle of 2∶1 pairing. Echocardiographic Z values including left coronary artery (LCA)-Z, right coronary artery (RCA)-Z, left anterior descending branch (LAD)-Z, left circumflex branch (LCX)-Z and CRP, ESR were compared between the two groups. General information of children with or without CAL were compared. The multivariate Logistic regression was used to screen the risk factors of IKD combined with CAL, and receiver operating characteristic (ROC) curve was used to analyze the predictive value of each index to IKD combined with CAL.Results:The LCA-Z, RCA-Z, LAD-Z, LCX-Z and the levels of CRP, ESR in the IKD group were higher than those in the control group: 3.26 ± 0.97 vs. 1.35 ± 0.28, 3.46 ± 0.92 vs. 1.01 ± 0.29, 3.18 ± 0.69 vs. 1.18 ± 0.34, 2.97 ± 0.68 vs.1.27 ± 0.34, (39.97 ± 9.03) mg/L vs. (4.21 ± 1.05) mg/L, (59.67 ± 16.34) mm/1 h vs. (12.85 ± 2.43) mm/1 h, there were statistical differences ( P<0.05). The duration of fever, intravenous immunoglobulin (IVIG) treatment response, IVIG start time, creatine kinase (CK), creatine kinase-MB(CK-MB), and N-terminal pro-B-type natriuretic peptide (NT-proBNP) between the children with IKD and CAL, IKD without CAL: (10.02 ± 2.45) d vs. (7.68 ± 1.43) d, 65.22%(15/23) vs. 88.89%(40/45), 60.87%(14/23) vs. 86.67%(39/45), (236.78 ± 59.74) U/L vs. (192.67 ± 35.41) U/L, (45.19 ± 9.85) U/L vs. (33.18 ± 9.87) U/L, (1.78 ± 0.32) μg/L vs. (0.92 ± 0.20) μg/L, there were statistical differences ( P<0.05). Logistic regression analysis showed that the duration of fever, IVIG treatment response, IVIG initiation time, CK, CK-MB, NT-proBNP, echocardiographic Z value, CRP and ESR levels were independent risk factors for IKD combined with CAL ( P<0.05). ROC curve analysis results showed that the area under the curve of IKD combined with CAL predicted by LCA-Z, RCA-Z, LADE-Z, LCX-Z, CRP and ESR was the largest, which was 0.917 (95% CI 0.824 - 0.970), which was higher than that diagnosed by each index alone ( P<0.05). Conclusions:There is a certain relationship between the echocardiographic Z values, CRP and ESR in children with IKD and CAL, which can be used to assist in the evaluation of IKD complicated with CAL.

Objective:To explore the relationship between coronary artery measurements combined with C-reactive protein(CRP), erythrocyte sedimentation rate(ESR) and coronary artery lesionsin(CAL) with incomplete Kawasaki disease (IKD) in children.Methods:Sixty-eight cases of pediatric IKD admitted to Huaibei Maternal and Child Health Care Hospital from January 2020 to April 2024 were selected as the IKD group, and 34 healthy children undergoing physical examination during the same period were selected as the control group according to the principle of 2∶1 pairing. Echocardiographic Z values including left coronary artery (LCA)-Z, right coronary artery (RCA)-Z, left anterior descending branch (LAD)-Z, left circumflex branch (LCX)-Z and CRP, ESR were compared between the two groups. General information of children with or without CAL were compared. The multivariate Logistic regression was used to screen the risk factors of IKD combined with CAL, and receiver operating characteristic (ROC) curve was used to analyze the predictive value of each index to IKD combined with CAL.Results:The LCA-Z, RCA-Z, LAD-Z, LCX-Z and the levels of CRP, ESR in the IKD group were higher than those in the control group: 3.26 ± 0.97 vs. 1.35 ± 0.28, 3.46 ± 0.92 vs. 1.01 ± 0.29, 3.18 ± 0.69 vs. 1.18 ± 0.34, 2.97 ± 0.68 vs.1.27 ± 0.34, (39.97 ± 9.03) mg/L vs. (4.21 ± 1.05) mg/L, (59.67 ± 16.34) mm/1 h vs. (12.85 ± 2.43) mm/1 h, there were statistical differences ( P<0.05). The duration of fever, intravenous immunoglobulin (IVIG) treatment response, IVIG start time, creatine kinase (CK), creatine kinase-MB(CK-MB), and N-terminal pro-B-type natriuretic peptide (NT-proBNP) between the children with IKD and CAL, IKD without CAL: (10.02 ± 2.45) d vs. (7.68 ± 1.43) d, 65.22%(15/23) vs. 88.89%(40/45), 60.87%(14/23) vs. 86.67%(39/45), (236.78 ± 59.74) U/L vs. (192.67 ± 35.41) U/L, (45.19 ± 9.85) U/L vs. (33.18 ± 9.87) U/L, (1.78 ± 0.32) μg/L vs. (0.92 ± 0.20) μg/L, there were statistical differences ( P<0.05). Logistic regression analysis showed that the duration of fever, IVIG treatment response, IVIG initiation time, CK, CK-MB, NT-proBNP, echocardiographic Z value, CRP and ESR levels were independent risk factors for IKD combined with CAL ( P<0.05). ROC curve analysis results showed that the area under the curve of IKD combined with CAL predicted by LCA-Z, RCA-Z, LADE-Z, LCX-Z, CRP and ESR was the largest, which was 0.917 (95% CI 0.824 - 0.970), which was higher than that diagnosed by each index alone ( P<0.05). Conclusions:There is a certain relationship between the echocardiographic Z values, CRP and ESR in children with IKD and CAL, which can be used to assist in the evaluation of IKD complicated with CAL.

OBJECTIVE: To investigate the effect of vitamin D3 on polarization of monocyte macrophages induced by serum from patients with ankylosing spondylitis (AS). METHODS: Twenty AS naïve patients and 20 healthy controls from Wenzhou People's Hospital during January 2016 and December 2017 were enrolled. The macrophages were differentiated from THP1 cells induced by phorbol 12-myristate 13-acetate (PMA), and then co-cultured with the serum from healthy subjects (control group) or AS patients. Vitamin D3 was added in the medium mixed with serum from AS patients. Flow cytometry was used to analyze the ratio of CD68 and CD206 positive cells, and RT-PCR was performed to detect the mRNA expression of inducible nitric oxide synthase(iNOS) and arginase-1(Arg-1). RESULTS: THP1 cells could be polarized into mononuclear-macrophages with the induction of PMA. The proportion of CD206 positive cells in AS-serum group was lower than that in the control group (=9.434, <0.05), while the proportion of CD68 positive cells was higher than that in the control group (=43.920, <0.05). The proportion of CD206 positive cells in vitamin D3 group was higher than that in AS-serum group (=8.895, <0.05), while the proportion of CD68 positive cells was lower than that in AS-serum group (=9.089, <0.05). mRNA expression of Arg-1 in AS-serum group was lower than that in the control group (=8.899, <0.05), while mRNA expression of iNOS was higher than that in the control group (=3.656, <0.05). mRNA expression of Arg-1 in vitamin D3 group was higher than that in AS-serum group (=6.219, <0.05), while mRNA expression of iNOS was lower than that in AS-serum group (=5.876, <0.05). CONCLUSIONS Vitamin D3 can regulate the polarization of mononuclear macrophages for immunoregulation in patients with AS.
Adjuvants, Immunologic ; pharmacology ; Cell Differentiation ; drug effects ; Cholecalciferol ; pharmacology ; Humans ; Monocytes ; drug effects ; Spondylitis, Ankylosing ; blood ; physiopathology

Objective To explore the prognostic value of ventricular wall motion score (WMS) and left ventricular ejection fraction (LVEF) in patients with acute myocardial infarction (AMI) combined with heart failure (HF) in the recent 12 months Methods We selected hospitalized AMI patients in our department from Jan. 2014 to Nov. 2014. Cardiac ultrasound was performed to detect WMS and LVEF within 48 hours and during the 6?month follow?up period and the occurrence of HF was recorded. Results Totally 127 AMI patients were recruited, including 20 cases combined with HF. The WMS was higher in HF group than those in non?HF group (23.55 ± 3.73 vs 20.11 ± 3.13, P<0.01), while the LVEF in HF group was lower than those in non?HF group (48.77 ± 8.08 vs 56.99 ± 5.17, P<0.01). Multiple logistic regression analysis revealed that WMS and LVEF were both independent predictors of HF in the recent 12 months (P < 0.05). The prediction effect of WMS ROC curve area was 0.81 (P < 0.01) , and LVEF 0.76 (P < 0.05). WMS and LVEF combined assessment area under ROC curve was 0.82 (P < 0.01). Conclusion WMS and LVEF are independent predictors of HF in patients with AMI in the recent 12 months and the combined application of WMS and LVEF can significantly improve the prediction effect.

Objective To investigate the effects of tripterygium glycosides combined with methotrexate in treatment of rheumatoid arthritis (RA) curative effect and peripheral blood of patients with IL-6, effects of IL-10 and TNF- alpha. Methods 80 patients with RA from February 2014 to August 2016 in our hospital were retrospectively analyzed. According to the different treatment methods by for the observation group, the control group (40 cases). To compare and analyze the curative effect before and after short-term treatment and treatment of patients in the 2 groups were the main clinical symptoms and signs of change and ESR, RF, CRP, IL-6, TNF- alpha, IL-10 index. Results The total effective rate of observation group (92.50％) was significantly higher than the control group (77.50％), the difference was statistically significant (P<0.05), 2 groups of patients before treatment of main clinical symptoms and signs, there was no significant difference, after treatment, clinical symptoms and signs of 2 groups of patients were compared with those before treatment was statistically significant to alleviate the differences (P<0.05), and the observation group of main clinical symptoms and signs were lower than the control group and the remission (down), the difference was statistically significant (P<0.05).Before ESR, the treatment of 2 patients with RF, CRP, TNF- IL-6, alpha, IL-10 index level, no statistical significance, the 2 groups after treatment in patients with ESR, RF, CRP, IL-6, TNF- alpha, IL-10 index level than before treatment with significant difference (P<0.05), and the observation group ESR, RF, CRP, TNF- alpha, IL-6 average index of water compared with the control group decreased significantly, IL-10 index increased significantly than the control group. The difference was statistically significant (P<0.05). Conclusion Tripterygium glycosides combined with methotrexate in treatment of rheumatoid arthritis And it can effectively inhibit the expression of peripheral serum L-6 and TNF- alpha, promote the expression of IL-10 and alleviate the inflammatory reaction

Objective The primary aim of this study was to examine the proportion and natural history of Helicobacter pylori (Hp) negative bleeding peptic ulcers. Methods The study was designed as a multiple-center, case-control study conducted in 14 endoscopy centers in China from April 2006 to March 2007. Each center was expected to recruit 30 peptic ulcer patients with bleeding ( PUB group) and 30 without (PU group). All screened patients with upper gastrointestinal bleeding received endoscopy within 24 hours of admission. Biopsy specimens were taken from the antrum to determine Hp infection by rapid urease test and pathology. Patients with negative Hp infection at first examination were asked to receive urease breathe test (UBT) one month later. Results A total of 617 patients were enrolled with 263 in PUB group and 354 in PU group. There is no significant difference in demographic characters between 2 groups ( P ＞0. 05). The rate of Hp infection in PUB group ( 161/263, 61.2% ) was significantly lower than that in PUgroup (311/354, 87. 9%, P ＜0. 001 ). The incidence of complex ulcer in Hp positive PUB patients was 7.5% ( 12/161 ), which is significantly higher than that in Hp negative PUB patients ( 1/102, 1.0% , P =0. 018). In PUB group, no significant differences were found between Hp positive and negative patients in regarding of age, sex, rates of haematemesis, duodenal ulcer and gastric ulcer, and size of ulcer ( P ＞0. 05 ). Among 102 Hp negative cases in PUB, no positive case was found in UBT one month later. Conclusion We have demonstrated a rise in the incidence of Hp negative bleeding ulcers in China. The idiopathic ulcer was not rare, and might have a higher tendency to cause bleed.