Objective:To investigate the expression of TMSB10 mRNA in primary liver can-cer and its clinical value.Methods:The primary liver cancer data set was downloaded from the TCGA database and to analyze the expression of TMSB10 mRNA in primary liver cancer.The as-sociation between TMSB10 mRNA expression and primary liver cancer patients survival rate,im-mune cell infiltration were analyzed.A total of 150 primary liver cancer tissues and adjacent tis-sues were enrolled in our hospital,and the expression of TMSB10 mRNA in primary liver cancer tissues and adjacent tissues were analyzed via immunohistochemistry.All patients with primary liver cancer were followed up and divide into poor prognosis(n=81)and good prognosis group(n=69).The Logistic regression analysis was used to analyze the risk factors for poor prognosis in pa-tients with primary liver cancer.Results:The expression of TMSB10 mRNA in primary liver can-cer tissue and normal liver tissue were 1111.239(601.842,2154.575),396.998(324.379,524.741),respectively.When compared with normal liver tissue,the TMSB10 mRNA was significantly over-expressed in primary liver cancer tissue(P＜0.001).The median survival time of the TMSB10 mRNA lower expression group was 84.4 months,while the median survival time was 49.7 months in TMSB10 mRNA higher expression group.Compared with the TMSB10 lower expression group,the survival rate of the TMSB10 higher expression group was significantly decreased(HR=1.55,P=0.039).The expression of TMSB10 mRNA in primary liver cancer tissue was significantly positively correlated with the infiltration degree of B lymphocytes,CD8+T lymphocytes,CD4+T lympho-cytes,macrophages,neutrophils,and dendritic cells.The high expression rate of TMSB10 in pri-mary liver cancer tissues was significantly higher than that in adjacent tissues(x2=103.419,P＜0.001).The Logistic analysis showed that vascular invasion,AJCC stage with Ⅲ-Ⅳ,and higher ex-pression of TMSB were all independent risk factors for the poor prognosis in patients with primary liver cancer.In poor prognosis group,when compared with TMSB10 lower expression group,the time of recurrence and metastasis in TMSB10 higher expression group was significantly shortened(t=4.456,P＜0.001).Conclusion:TMSB10 was significantly over-expressed in primary liver cancer,and TMSB can serve as a biological marker for poor prognosis in patients with primary liver cancer.

Objective:To investigate the expression of TMSB10 mRNA in primary liver can-cer and its clinical value.Methods:The primary liver cancer data set was downloaded from the TCGA database and to analyze the expression of TMSB10 mRNA in primary liver cancer.The as-sociation between TMSB10 mRNA expression and primary liver cancer patients survival rate,im-mune cell infiltration were analyzed.A total of 150 primary liver cancer tissues and adjacent tis-sues were enrolled in our hospital,and the expression of TMSB10 mRNA in primary liver cancer tissues and adjacent tissues were analyzed via immunohistochemistry.All patients with primary liver cancer were followed up and divide into poor prognosis(n=81)and good prognosis group(n=69).The Logistic regression analysis was used to analyze the risk factors for poor prognosis in pa-tients with primary liver cancer.Results:The expression of TMSB10 mRNA in primary liver can-cer tissue and normal liver tissue were 1111.239(601.842,2154.575),396.998(324.379,524.741),respectively.When compared with normal liver tissue,the TMSB10 mRNA was significantly over-expressed in primary liver cancer tissue(P＜0.001).The median survival time of the TMSB10 mRNA lower expression group was 84.4 months,while the median survival time was 49.7 months in TMSB10 mRNA higher expression group.Compared with the TMSB10 lower expression group,the survival rate of the TMSB10 higher expression group was significantly decreased(HR=1.55,P=0.039).The expression of TMSB10 mRNA in primary liver cancer tissue was significantly positively correlated with the infiltration degree of B lymphocytes,CD8+T lymphocytes,CD4+T lympho-cytes,macrophages,neutrophils,and dendritic cells.The high expression rate of TMSB10 in pri-mary liver cancer tissues was significantly higher than that in adjacent tissues(x2=103.419,P＜0.001).The Logistic analysis showed that vascular invasion,AJCC stage with Ⅲ-Ⅳ,and higher ex-pression of TMSB were all independent risk factors for the poor prognosis in patients with primary liver cancer.In poor prognosis group,when compared with TMSB10 lower expression group,the time of recurrence and metastasis in TMSB10 higher expression group was significantly shortened(t=4.456,P＜0.001).Conclusion:TMSB10 was significantly over-expressed in primary liver cancer,and TMSB can serve as a biological marker for poor prognosis in patients with primary liver cancer.

Objective To investigate the clinical value of the combined detection of manganese superoxide dismutase (Mn-SOD)genetic polymorphism and lipoprotein phospholipase A2 (Lp-PLA2)in the early diagnosis of coronary heart disease (CHD).Methods 92 cases of coronary heart disease patients and 78 cases of healthy control group were selected.The activ-ity of Lp-PLA2,the activity of Mn-SOD and genotype of Mn-SOD 9 Ala/Val genetic polymorphism were detected in the ser-um of each group via the use of colorimetry,continuous monitoring technique and gene-sequencing method respectively and then the correlation of serum Mn-SOD,Lp-PLA2 and Mn-SOD genetic polymorphism with CHD were analyzed.Results The Lp-PLA2 activity in serum of CHD groups with Mn-SOD 9 VV genotype was statistically significantly higher than that of patients with the AV+AA genotype (P<0.01).The serum Mn-SOD activity in patients with VV genotype was signifi-cantly lower than that of those with AV+AA genotype (P<0.01).Conclusion Combined detection of Mn-SOD 9 Ala/Val genetic polymorphism and Lp-PLA2 activityin the serum can provide an important foundation for the diagnosis and predic-tion of coronary heart disease.