This is a report of 8-months progression of circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in Brunei Darussalam from November 2021. Whole-genome sequencing (WGS) was performed on nearly 3,000 random local COVID-19 patients in samples with a cycle-threshold (CT) of less than 25. Phylogenetic analysis revealed that the Delta variant, particularly AY.24.1, was the predominant variant towards the end of 2021. The same variant was then completely superseded by the Omicron variants in mid-January 2022. During the early wave of Omicron variants, BA.2.3.19 and its paternal BA.2.3 were found to be the prominent sublineage until it was replaced by BA.5 lineages four months later, mainly BA.5.2 and BF.5. Further analysis revealed that AY.24.1 and BA.2.3.19 that was once a prevalent variant are predominantly observed in Brunei. Both variants can be differentiated by the presence of a particular mutation in the S gene that encodes the viral spike protein that is essential for host cell interaction. Brunei is continuously monitoring the circulating SARS-CoV-2 variants as part of its genomic surveillance following the World Health Organization (WHO) guidance for surveillance.

Introduction: Mental health problem is an important public health issue which causes deterioration in quality of life, function and financial capabilities. According to the National Health and Morbidity Survey (NHMS) 1996, the prevalence of mental problem among adults in Malaysia was 10.7% and this has increased to 29.2% in NHMS 2015. The aim of this study was to evaluate the burden of depression, anxiety and stress among adults in a city in Klang Valley, Malaysia including its associated factors. Methods: A cross-sectional study was conducted by analysing secondary data obtained from screening program performed in six primary health clinics in the city throughout 2016. There were 576 adults participated in the screening program which consisted of mental health screening using Depression, Anxiety, Stress 21 (DASS-21) questionnaire and health status screening using Health Status Screening Form. Results: The prevalence of depression was 20.5%, anxiety was 44.5%, while stress was 10.1%. By using logistic regression, history of being abused was the only significant protective factor associated with depression, anxiety and stress with OR of 0.062 (95% CI 0.007; 0.563, p=0.004), OR of 0.001 (95% CI 0.000; -, p=0.001) and OR of 0.026 (95% CI 0.003; 0.238, p<0.001) respectively. Other independent variables were not statistically significant. Conclusions: As conclusions, surprisingly the risk of depression, anxiety and stress were not increased among participants who reported history of being abused. This was probably due to good coping mechanism among them. Nevertheless, early intervention needs to be emphasized to prevent mental disorders among this group of people in community.