Congenital cytomegalovirus (cCMV) infection is a major cause of neurodevelopmental impairment and sensorineural hearing loss worldwide, particularly in low- and middle-income countries where standardized molecular screening programs are not routinely implemented. Early laboratory confirmation within the first 21 days of life is essential to differentiate congenital from postnatal infection. This study evaluated the diagnostic agreement between the Simplexa cCMV Direct real-time PCR assay and conventional nested PCR for detecting CMV DNA in neonatal urine specimens in Indonesia. A cross-sectional diagnostic agreement study was conducted between September and December 2023 at a tertiary referral hospital. Urine samples from 87 neonates aged < 21 days with clinical suspicion of congenital infection were tested using both assays. The Simplexa assay detected 29 positive and 58 negative samples, while conventional nested PCR identified 28 positive and 59 negative samples. Agreement was assessed using Cohen’s kappa coefficient, McNemar’s test, and positive and negative agreement rates. The assays demonstrated near-perfect agreement (k = 0.87; 95% CI: 0.75–0.98; p < 0.001). Positive and negative agreement were 91.2% and 95.7%, respectively, with no statistically significant difference between methods (McNemar p = 1.00). Simplexa Direct real-time PCR showed high agreement with conventional nested PCR while offering operational advantages including simplified workflow and reduced contamination risk. These findings support the implementation of automated direct molecular platforms for neonatal cCMV diagnosis in tropical and resource-limited healthcare settings.

Introduction: The need for ARV (Anti Retro Viral) for the population aged 15-49 years increased from 145,706 in 2011 to 248,903 in 2016. People with HIV/AIDS who received ARVs were estimated at 16.60% in 2011 increased to 18% in 2016. In Indonesia up to September 2014, there were 108,060 people with HIV/AIDS who received ARV therapy from 33 provinces and 300 districts / cities. There were 19,670 people with HIV/AIDS who stopped ARV, 15,046 were not followed up due to various causes and as many as 14,547 died. Methods: This research used a quasi-experimental methods with non equivalent control group design and used a pretest, posttest 1 and posttest 2. There was two groups, the first group was treated using SMS (Short Message Service) reminder and leaflets and the second group was treated with the usual method (counselling). Results: Research with the Mann-Whitney statistical test shows that after 1 month of treatment, there was differences in the average score of knowledge (p value = 0.001), attitude (p value = 0.008), and adherence of Antiretroviral Consumption (p value = 0.010) between the intervention group and the control group. After 2 months of treatment, there was differences in the average knowledge score (p value = 0.001), attitude (p value = 0.001), and adherence of Antiretroviral Consume (p value = 0.001) between the intervention group and the control group. Conclusion: There is the interventions that able to improve the knowledge, attitudes and adherence of taking antiretroviral at PLWHA.

Introduction: The ability of nurses to conduct triage greatly influences the success rate of help when patients experience emergency. The aim of the study was to determine the factors associated with the accuracy of the implementation of triage in the X Hospital's Emergency Departments. Methods: The research method that the researcher used was cross sectional approach. The number of samples were 32 respondents. Questionnaire and observation were used to collect the data. Results: Respondents characteristics (age, sex, education, and tenure) with the accuracy of the triage were obtained by age with p value = 0.386, p value = 1,000 for gender, p value = 0.242 for education, and years of work with p value = 0.000162. Knowledge of respondents with the implementation of the determination of triage p value = 0.045 and motivation of respondents with the accuracy of the implementation of triage p value = 0.0017. Conclusion: The accuracy of the implementation of triage has no relationship with characteristics (age, sex, education), there is a relationship between knowledge, motivation and years of work with the implementation's accuracy of triage in the Emergency Room X Hospital. Suggestions are expected by the Hospital to make guidelines, triage algorithms, training, regeneration to improve nurse triage knowledge.

One of the vascular disorders that occurs in the elderly is hypertension. The most severe consequence of this complications is stroke. Prevention of hypertension with non-pharmacological therapy like listening to classical music (eg. Mozart). Classical music can stimulate alpha waves in the brain, releasing a peptide called beta-endorphin. This causes a sense of tranquility so that the heart rhythm decreases, and blood pressure drops. The sample in this study were 17 respondents, aged over 60 years. The result of this case report showed an average systolic reduction of 21 mmHg. Before intervention, 53% suffered from stage 2 hypertension (mean systolic 162 mmHg) and after the intervention with classical music therapy for 12 days, 65% recovered with stage 1 hypertension (average systolic 141 mmHg). So, the interventions of listening to classical music, can maintain balance in blood pressure among hypertension patient.