By establishing a complete data organizational structure,technical architecture,quality control framework,and concept framework,hospitals can effectively regulate data management,data security,and quality monitoring,achieving full-cycle monitoring of data management.Breaking down the resource barriers of data systems,improving the efficiency of data usage and circulation,and promoting the increase in data value.It drives the hospital's scientific research innovation,medical insurance cost control,data value monetization,and the improvement of high-quality capabilities.Through the establishment of a sustainable digital culture and operational philosophy,it integrates data with hospital assets,continuously enhancing the value realization of hospital data in operations,management,diagnosis and treatment,and scientific research.

Objective To investigate the in vitro antibacterial effect of Radix Sophorae Flavescentis on tigecycline-resistant carbapenem-resistant Klebsiella pneumonia(CRKP)and elucidate how Radix Sophorae Flavescentis restores the sensitivity of CRKP to tigecycline through the efflux pump pathway.Methods The CRKP standard strain BAA1705 was induced to be tigecycline-resistant using the microdilution broth method,and the expression of efflux pumps was verified by efflux pump inhibitors.The fractional inhibitory concentration index(FIC)was calculated using the microdilution checkerboard method to determine the minimum inhibitory concertration(MIC)of the two drugs in combination.Fluorescent real-time quantitative PCR(qRT-PCR)was used to analyze the expression of the AcrB gene before and after Radix Sophorae Flavescentis treatment,and the e?ux pump activity was validated by the Rhodamine 6G e?ux assay.Results The e?ux pump inhibition experiment on the artificially induced T-BAA1705 strain showed overexpression of e?ux pumps.The combined antibacterial test results indicated synergy between Radix Sophorae Flavescentis and tigecycline(FIC=0.375 2).The qRT-PCR results showed that the AcrB levels in the Radix Sophorae Flavescentis monotherapy group were lower than in the untreated group(0.018±0.002 vs 0.040±0.001),and the AcrB levels(0.009±0.001),RamA levels(0.184±0.003)and MarA(0.013±0.001)levels in the combination therapy group were all lower than in the untreated group(0.040±0.001,0.387±0.016,0.125±0.007),while the AcrR level was higher than in the untreated group(0.388±0.001 vs 0.288±0.001),and the differences were statistical significance(t=12.28～47.12,all P<0.05);the e?ux pump activity in the Radix Sophorae Flavescentis monotherapy group and the combination therapy group was lower than in the untreated T-BAA1705(60.667±0.448,60.267±0.376,65.133±0.296),and the differences were statistical significance(t=8.31,10.17,all P<0.05).Conclusion Radix Sophorae Flavescentis can restore the sensitivity of tigecycline-resistant CRKP to tigecycline and effectively inhibit bacterial growth when used in combination with tigecycline.The mechanism may involve Radix Sophorae Flavescentis inhibiting the expression of the AcrAB-TolC e?ux pump.

Objective To investigate the in vitro antibacterial effect of Radix Sophorae Flavescentis on tigecycline-resistant carbapenem-resistant Klebsiella pneumonia(CRKP)and elucidate how Radix Sophorae Flavescentis restores the sensitivity of CRKP to tigecycline through the efflux pump pathway.Methods The CRKP standard strain BAA1705 was induced to be tigecycline-resistant using the microdilution broth method,and the expression of efflux pumps was verified by efflux pump inhibitors.The fractional inhibitory concentration index(FIC)was calculated using the microdilution checkerboard method to determine the minimum inhibitory concertration(MIC)of the two drugs in combination.Fluorescent real-time quantitative PCR(qRT-PCR)was used to analyze the expression of the AcrB gene before and after Radix Sophorae Flavescentis treatment,and the e?ux pump activity was validated by the Rhodamine 6G e?ux assay.Results The e?ux pump inhibition experiment on the artificially induced T-BAA1705 strain showed overexpression of e?ux pumps.The combined antibacterial test results indicated synergy between Radix Sophorae Flavescentis and tigecycline(FIC=0.375 2).The qRT-PCR results showed that the AcrB levels in the Radix Sophorae Flavescentis monotherapy group were lower than in the untreated group(0.018±0.002 vs 0.040±0.001),and the AcrB levels(0.009±0.001),RamA levels(0.184±0.003)and MarA(0.013±0.001)levels in the combination therapy group were all lower than in the untreated group(0.040±0.001,0.387±0.016,0.125±0.007),while the AcrR level was higher than in the untreated group(0.388±0.001 vs 0.288±0.001),and the differences were statistical significance(t=12.28～47.12,all P<0.05);the e?ux pump activity in the Radix Sophorae Flavescentis monotherapy group and the combination therapy group was lower than in the untreated T-BAA1705(60.667±0.448,60.267±0.376,65.133±0.296),and the differences were statistical significance(t=8.31,10.17,all P<0.05).Conclusion Radix Sophorae Flavescentis can restore the sensitivity of tigecycline-resistant CRKP to tigecycline and effectively inhibit bacterial growth when used in combination with tigecycline.The mechanism may involve Radix Sophorae Flavescentis inhibiting the expression of the AcrAB-TolC e?ux pump.

By establishing a complete data organizational structure,technical architecture,quality control framework,and concept framework,hospitals can effectively regulate data management,data security,and quality monitoring,achieving full-cycle monitoring of data management.Breaking down the resource barriers of data systems,improving the efficiency of data usage and circulation,and promoting the increase in data value.It drives the hospital's scientific research innovation,medical insurance cost control,data value monetization,and the improvement of high-quality capabilities.Through the establishment of a sustainable digital culture and operational philosophy,it integrates data with hospital assets,continuously enhancing the value realization of hospital data in operations,management,diagnosis and treatment,and scientific research.

In the field of health,with the innovation drive as the core,the new quality of productivity has successfully gets rid of the shackles of traditional health service delivery mode and industrial development trajectory,showing high-tech,high-efficiency and high-quality characteristics,which is in line with the development concept advocated in the new development stage,representing an advanced productivity quality.In the field of health economics,the new-quality productivity will have a decisive impact on the supply and demand of health services,the allocation and optimization of health resources,health policies and intervention measurement,health system performance and health equity.Meanwhile,the new quality productivity has a profound impact on health economic evaluation and technology evaluation,and even changes the health economic research paradigm.It is undoubtedly an important responsibility and challenge for the health economy research field to give timely and in-depth theoretical discussion on the new quality productivity and actively promote its practical application and development.It requires us to keep up with the pace of the times at the theoretical level and dig deep into the inherent law and value of the new-quality productivity.At the same time,in practice,it is committed to guiding and promoting the effective transformation and implementation of new-quality productivity in the field of health,so as to facilitate the elevation of health economic research and practice to a new qualitative level.

In the field of health,with the innovation drive as the core,the new quality of productivity has successfully gets rid of the shackles of traditional health service delivery mode and industrial development trajectory,showing high-tech,high-efficiency and high-quality characteristics,which is in line with the development concept advocated in the new development stage,representing an advanced productivity quality.In the field of health economics,the new-quality productivity will have a decisive impact on the supply and demand of health services,the allocation and optimization of health resources,health policies and intervention measurement,health system performance and health equity.Meanwhile,the new quality productivity has a profound impact on health economic evaluation and technology evaluation,and even changes the health economic research paradigm.It is undoubtedly an important responsibility and challenge for the health economy research field to give timely and in-depth theoretical discussion on the new quality productivity and actively promote its practical application and development.It requires us to keep up with the pace of the times at the theoretical level and dig deep into the inherent law and value of the new-quality productivity.At the same time,in practice,it is committed to guiding and promoting the effective transformation and implementation of new-quality productivity in the field of health,so as to facilitate the elevation of health economic research and practice to a new qualitative level.

In the field of health,with the innovation drive as the core,the new quality of productivity has successfully gets rid of the shackles of traditional health service delivery mode and industrial development trajectory,showing high-tech,high-efficiency and high-quality characteristics,which is in line with the development concept advocated in the new development stage,representing an advanced productivity quality.In the field of health economics,the new-quality productivity will have a decisive impact on the supply and demand of health services,the allocation and optimization of health resources,health policies and intervention measurement,health system performance and health equity.Meanwhile,the new quality productivity has a profound impact on health economic evaluation and technology evaluation,and even changes the health economic research paradigm.It is undoubtedly an important responsibility and challenge for the health economy research field to give timely and in-depth theoretical discussion on the new quality productivity and actively promote its practical application and development.It requires us to keep up with the pace of the times at the theoretical level and dig deep into the inherent law and value of the new-quality productivity.At the same time,in practice,it is committed to guiding and promoting the effective transformation and implementation of new-quality productivity in the field of health,so as to facilitate the elevation of health economic research and practice to a new qualitative level.

In the field of health,with the innovation drive as the core,the new quality of productivity has successfully gets rid of the shackles of traditional health service delivery mode and industrial development trajectory,showing high-tech,high-efficiency and high-quality characteristics,which is in line with the development concept advocated in the new development stage,representing an advanced productivity quality.In the field of health economics,the new-quality productivity will have a decisive impact on the supply and demand of health services,the allocation and optimization of health resources,health policies and intervention measurement,health system performance and health equity.Meanwhile,the new quality productivity has a profound impact on health economic evaluation and technology evaluation,and even changes the health economic research paradigm.It is undoubtedly an important responsibility and challenge for the health economy research field to give timely and in-depth theoretical discussion on the new quality productivity and actively promote its practical application and development.It requires us to keep up with the pace of the times at the theoretical level and dig deep into the inherent law and value of the new-quality productivity.At the same time,in practice,it is committed to guiding and promoting the effective transformation and implementation of new-quality productivity in the field of health,so as to facilitate the elevation of health economic research and practice to a new qualitative level.

In the field of health,with the innovation drive as the core,the new quality of productivity has successfully gets rid of the shackles of traditional health service delivery mode and industrial development trajectory,showing high-tech,high-efficiency and high-quality characteristics,which is in line with the development concept advocated in the new development stage,representing an advanced productivity quality.In the field of health economics,the new-quality productivity will have a decisive impact on the supply and demand of health services,the allocation and optimization of health resources,health policies and intervention measurement,health system performance and health equity.Meanwhile,the new quality productivity has a profound impact on health economic evaluation and technology evaluation,and even changes the health economic research paradigm.It is undoubtedly an important responsibility and challenge for the health economy research field to give timely and in-depth theoretical discussion on the new quality productivity and actively promote its practical application and development.It requires us to keep up with the pace of the times at the theoretical level and dig deep into the inherent law and value of the new-quality productivity.At the same time,in practice,it is committed to guiding and promoting the effective transformation and implementation of new-quality productivity in the field of health,so as to facilitate the elevation of health economic research and practice to a new qualitative level.

In the field of health,with the innovation drive as the core,the new quality of productivity has successfully gets rid of the shackles of traditional health service delivery mode and industrial development trajectory,showing high-tech,high-efficiency and high-quality characteristics,which is in line with the development concept advocated in the new development stage,representing an advanced productivity quality.In the field of health economics,the new-quality productivity will have a decisive impact on the supply and demand of health services,the allocation and optimization of health resources,health policies and intervention measurement,health system performance and health equity.Meanwhile,the new quality productivity has a profound impact on health economic evaluation and technology evaluation,and even changes the health economic research paradigm.It is undoubtedly an important responsibility and challenge for the health economy research field to give timely and in-depth theoretical discussion on the new quality productivity and actively promote its practical application and development.It requires us to keep up with the pace of the times at the theoretical level and dig deep into the inherent law and value of the new-quality productivity.At the same time,in practice,it is committed to guiding and promoting the effective transformation and implementation of new-quality productivity in the field of health,so as to facilitate the elevation of health economic research and practice to a new qualitative level.