ObjectiveTo establish a tumor prognostic risk assessment model related to target genes of the miR-34 family. MethodsTarget genes of the miR-34 family were screened， and the scores of miR-34 target genes were assessed in 16 tumor types. Univariate Cox regression analysis was used to identify the tumor type with the strongest correlation between miR-34 target gene scores and overall survival （OS）. Gene Ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） analyses were performed to elucidate the functional roles and signaling pathways of miR-34 target genes. A prognostic risk model based on the miR-34 target genes was constructed using univariate Cox and LASSO regression analyses. Quantitative real-time PCR （qPCR） and dual-luciferase reporter assays were conducted to validate whether the target genes bind to miR-34 and measure their RNA expression levels in the relevant tumors. Additionally， the risk score was integrated with other clinical indicators to develop a nomogram prediction model for patient survival. ResultsA total of 65 target genes of the miR-34 family were screened. The cancer type exhibiting stronger correlation between the target gene scores and OS was lung adenocarcinoma （P = 0.003， HR= 5.150）. Furthermore， miR-34 target genes were predominantly enriched in oxidative stress pathways and various tumor-related processes. Three genes， LDHA， GALNT7， and SATB2， were identified as core components of the prognostic analysis model for lung adenocarcinoma. Additionally， the constructed nomogram model demonstrated robust predictive performance. ConclusionThe risk model and prognosis model of lung adenocarcinoma constructed based on the key target genes of miR-34 have good predictive performance.

Objective To explore the risk of preeclampsia(PE)and construct an individualized column chart predictive model.Methods A total of 209 PE patients admitted to Huzhou Maternal and Child Health Hospital from January 2020 to July 2023 were selected as research group,and 162 healthy pregnant women who underwent prenatal examination during the same period were selected as control group.Logistic regression model was used for data analysis,and nomogram prediction model based on Logistic regression results was constructed by R statistical software,receiver operating characteristic(ROC)curve and calibration curve were drawn,and Hosmer-Lemeshow goodness-of-fit test was conducted to evaluate prediction efficiency.Results The occurrence of PE had no correlation with body mass index(BMI),gestational age,pregnancy times,abortion history and whether there are multiple pregnancies(P＞0.05),but age,education level,gestational diabetes,gestational hypertension and standardized prenatal examination of pregnant women are related factors(P＜0.05).Age≥30 years old,education below senior high school,gestational diabetes,pregnancy-induced hypertension and irregular prenatal examination were risk factors for PE(P＜0.05).The area under ROC curve of nomograph model was 0.813(95％CI:0.770-0.855).Conclusion The nomogram model based on age,education level,diabetes in pregnancy,hypertension in pregnancy and irregular prenatal examination screened by Logistic regression model has a good predictive effect on the occurrence of PE.

Objective To evaluate the prognosis of infants with common Hirschsprung's disease who undergo laparoscopic-assisted Soave procedure below 6 months of age,and to explore its feasibility.Methods A retrospective analysis was conducted on the clinical data of 82 cases of common Hirschsprung's disease admitted to our department from January 2018 to August 2022.The patients were divided into two groups based on their age at the time of surgery:Young surgical age group(＜6 months,50 cases)and Old surgical age group(≥ 6 months,32 cases).All patients underwent the treatment of laparoscopic-assisted Soave procedure.The surgical time,length of hospital stay,postoperative complications,and anal function were compared between the two groups.Results There were no significant differences between young surgical age group and old surgical age group in terms of surgical time[(145.04±11.21)min vs.(149.25±12.18)min,P=0.20],length of hospital stay[(13.56+0.93)d vs.(13.91±0.99)d,P=0.55],postoperative complications[22.00％(11/50)vs.28.13％(9/32),P=0.53],and Rintala score for anal function[(18.58±1.44)vs.(17.22±1.64),P=0.06].The main postoperative complications,including enterocolitis[10.00％(5/50)vs.12.50％(4/32),P=0.72],fecal/anal incontinence[6.00％(3/50)vs.6.25％(2/32),P=0.96],and constipation[4.00％(2/50)and 6.25％(2/32),P=0.65],showed no significant differences between the two groups(all P＞0.05).However,there was a statistically significant difference in terms of bleeding volume[(7.86±2.02)ml vs.(9.13±2.17)ml,P＜0.05].No deaths occurred in this study.Conclusion Laparoscopic-assisted Soave procedure is safe and effective for infants below 6 months of age with common congenital Hirschsprung's disease.It can achieve satisfactory anal function and reduce the incidence of adverse events during the waiting period for surgery.

Catalpol,the primary active component of Rehmannia glutinosa,exhibits potent antioxidant and anti-inflammatory effects and regulatory effects on glucose and lipid metabolism.This article systematically reviews recent studies on the impact of catalpol in addressing glycolipid metabolic disorders and related diseases and the underlying mechainsms.Catalpol can correct glucose and lipid metabolism imbalances to prevent the development and progression of diabetes complications including macrovascular and microvascu-lar diseases,through modulating the adenosine 5'-monophosphate-activated protein kinase signaling pathway to regulate lipogenesis and fat oxidation and altering the phosphorylation of forkhead transcription factor 1 and glycogen synthase kinase 3 to influence glyco-gen synthesis and breakdown.Catalpol plays a protective role in the cardio-cerebrovascular system,renal function,and retinal struc-ture and function through mediating signaling pathways such as phosphatidylinositol 3-kinase/protein kinase B and oxidized low-density lipoprotein/lectin-like oxidized low-density lipoprotein receptor-1.By suppressing the nuclear factor kappa-light-chain-enhancer of activated B cells pathway and endoplasmic reticulum stress,catalpol can alleviate inflammatory responses,thereby mitigat-ing inflammation-induced insulin resistance and glycolipid metabolic disorders.

Objective To explore the intention of chronic disease patients to use internet hospitals and its influencing factors based on the theory of planned behavior.Methods A convenience sampling survey was conducted using a questionnaire based on theory of planned behavior from July to October 2024.Structural equation modeling was employed to analyze the determinants of behavioral intention.Results The mean behavioral intention score was(96.25±11.62),indicating a moderate-to-high level.Structural equation modeling revealed significantly positive effects of subjective norms(β=0.696,P＜0.05)and perceived behavioral control(β=0.175,P＜0.05)on behavioral intention,while significant negative effects were observed for information risk perception(β=-0.286,P＜0.05)and medical risk perception(β=-0.221,P＜0.05).Conclusion Chronic disease patients demonstrate moderate-to-high intention to use internet hospitals.Key determinants of behavioral intention include subjective norms,perceived behavioral control,information risk perception,and medical risk perception.

Objective:To explore the application effect of interventional teaching in undergraduate nursing internship in operating rooms.Methods:A total of 98 undergraduate nursing students who worked as interns in the operating room during the two school years from June 2022 to February 2024 were selected as research subjects. Students who entered the program from June 2022 to February 2023 were enrolled into control group (50 students), and those who entered from June 2023 to February 2024 were enrolled into experimental group (48 students). The students conducted their internships in batches (with 4 to 5 students per subgroup) following a departmental rotation mechanism. The control group received routine teaching, while the experimental group received interventional teaching. At the end of the internship, the two groups were compared for their performance, including basic and specialized knowledge, skills, theory, and operation; patient reception, tripartite verification, and intraoperative cooperation for planned video-assisted thoracoscopic bullectomy; and their degrees of satisfaction with the teaching. The t-test was performed using SPSS 26.0. Results:The experimental group achieved significantly higher theoretical scores than the control group [(91.69±4.51) vs. (74.52±6.06)]. Meanwhile, the experimental group was significantly superior to the control group in operational performance, including surgical hand disinfection [(96.08±1.93) vs. (92.14±3.07)] and wearing and removing sterile surgical gowns and sterile gloves [(97.00±1.75) vs. (94.10±3.48)). In terms of specialized knowledge and skills, specifically, patient reception and third-party verification in simulated surgical nursing, the experimental group achieved higher scores than the control group [(98.77±0.69) vs. (94.04±2.56); (98.54±0.65) vs. (94.64±2.65)]. In terms of intraoperative cooperation, the experimental group showed a slight advantage over the control group. The experimental group had higher degrees of satisfaction with teaching than the control group, including course design, implementation methods, course arrangement, teaching attitude, teaching methods, degree of participation, gains, and assistance.Conclusions:After adopting interventional teaching, the teaching effect for nursing internship in operating rooms is better. It improves the various assessment scores of nursing students, enhances their comprehensive abilities and degrees of satisfaction with teaching, and reinforces the teaching abilities in operating rooms.

Objective To explore the intention of chronic disease patients to use internet hospitals and its influencing factors based on the theory of planned behavior.Methods A convenience sampling survey was conducted using a questionnaire based on theory of planned behavior from July to October 2024.Structural equation modeling was employed to analyze the determinants of behavioral intention.Results The mean behavioral intention score was(96.25±11.62),indicating a moderate-to-high level.Structural equation modeling revealed significantly positive effects of subjective norms(β=0.696,P＜0.05)and perceived behavioral control(β=0.175,P＜0.05)on behavioral intention,while significant negative effects were observed for information risk perception(β=-0.286,P＜0.05)and medical risk perception(β=-0.221,P＜0.05).Conclusion Chronic disease patients demonstrate moderate-to-high intention to use internet hospitals.Key determinants of behavioral intention include subjective norms,perceived behavioral control,information risk perception,and medical risk perception.

Objective To investigate the status and influencing factors of the coexistence of multiple frailty domains in patients with chronic heart failure(CHF),and to provide a reference for formulating targeted intervention measures.Methods The patients with CHF hospitalized in the cardiovascular department of a tertiary general hospital in Tianjin from November 2021 to December 2022 were conveniently selected as the study subjects.The self-designed general information questionnaire,FRAIL scale,Montreal Cognitive Assessment(MoCA)scale,HALFT Scale,Social Support Rating Scale and Self-Rating Depression Scale were used to investigate the coexistence of physical,cognitive and social frailty in CHF patients,and single factor analysis and binary logistic regression analysis were used to analyze the influencing factors.Results A total of 275 questionnaires were issued and 269 valid questionnaires were collected.Among 269 patients with CHF,12(4.5％),67(24.9％),122(45.4％)and 68(25.3％)patients had 0,1,2 and 3 frailty domains,respectively.According to the patients with CHF whether coexists physical,cognitive,social frailty,divided into coexistence and non-coexistence group.In univariate analysis,there were statistically significant differences in education,residence,family history of cardiovascular disease,NYHA cardiac function grade,atrial fibrillation,BMI,social support score and depression score(P＜0.05).Binary logistic regression analysis showed that high education level(OR=0.567,95％CI 0.397～0.810,P=0.002)was the protective factor for multiple frailty domains in CHF patients,and atrial fibrillation(OR=1.904,95％CI 1.054～3.442,P=0.033)and depression(OR=1.038,95％CI 1.006～1.071,P=0.018)were risk factors.Conclusion The prevalence of the coexistence of multiple frailty domains in CHF patients is high.Education level,atrial fibrillation and depression are potential intervention targets for multiple frailty domains in CHF patients.Targeted intervention measures should be carried out to reduce the incidence of multiple frailty domains and to improve quality of life for CHF patients.

Objective To investigate the status and influencing factors of the coexistence of multiple frailty domains in patients with chronic heart failure(CHF),and to provide a reference for formulating targeted intervention measures.Methods The patients with CHF hospitalized in the cardiovascular department of a tertiary general hospital in Tianjin from November 2021 to December 2022 were conveniently selected as the study subjects.The self-designed general information questionnaire,FRAIL scale,Montreal Cognitive Assessment(MoCA)scale,HALFT Scale,Social Support Rating Scale and Self-Rating Depression Scale were used to investigate the coexistence of physical,cognitive and social frailty in CHF patients,and single factor analysis and binary logistic regression analysis were used to analyze the influencing factors.Results A total of 275 questionnaires were issued and 269 valid questionnaires were collected.Among 269 patients with CHF,12(4.5％),67(24.9％),122(45.4％)and 68(25.3％)patients had 0,1,2 and 3 frailty domains,respectively.According to the patients with CHF whether coexists physical,cognitive,social frailty,divided into coexistence and non-coexistence group.In univariate analysis,there were statistically significant differences in education,residence,family history of cardiovascular disease,NYHA cardiac function grade,atrial fibrillation,BMI,social support score and depression score(P＜0.05).Binary logistic regression analysis showed that high education level(OR=0.567,95％CI 0.397～0.810,P=0.002)was the protective factor for multiple frailty domains in CHF patients,and atrial fibrillation(OR=1.904,95％CI 1.054～3.442,P=0.033)and depression(OR=1.038,95％CI 1.006～1.071,P=0.018)were risk factors.Conclusion The prevalence of the coexistence of multiple frailty domains in CHF patients is high.Education level,atrial fibrillation and depression are potential intervention targets for multiple frailty domains in CHF patients.Targeted intervention measures should be carried out to reduce the incidence of multiple frailty domains and to improve quality of life for CHF patients.

Objective To evaluate the prognosis of infants with common Hirschsprung's disease who undergo laparoscopic-assisted Soave procedure below 6 months of age,and to explore its feasibility.Methods A retrospective analysis was conducted on the clinical data of 82 cases of common Hirschsprung's disease admitted to our department from January 2018 to August 2022.The patients were divided into two groups based on their age at the time of surgery:Young surgical age group(＜6 months,50 cases)and Old surgical age group(≥ 6 months,32 cases).All patients underwent the treatment of laparoscopic-assisted Soave procedure.The surgical time,length of hospital stay,postoperative complications,and anal function were compared between the two groups.Results There were no significant differences between young surgical age group and old surgical age group in terms of surgical time[(145.04±11.21)min vs.(149.25±12.18)min,P=0.20],length of hospital stay[(13.56+0.93)d vs.(13.91±0.99)d,P=0.55],postoperative complications[22.00％(11/50)vs.28.13％(9/32),P=0.53],and Rintala score for anal function[(18.58±1.44)vs.(17.22±1.64),P=0.06].The main postoperative complications,including enterocolitis[10.00％(5/50)vs.12.50％(4/32),P=0.72],fecal/anal incontinence[6.00％(3/50)vs.6.25％(2/32),P=0.96],and constipation[4.00％(2/50)and 6.25％(2/32),P=0.65],showed no significant differences between the two groups(all P＞0.05).However,there was a statistically significant difference in terms of bleeding volume[(7.86±2.02)ml vs.(9.13±2.17)ml,P＜0.05].No deaths occurred in this study.Conclusion Laparoscopic-assisted Soave procedure is safe and effective for infants below 6 months of age with common congenital Hirschsprung's disease.It can achieve satisfactory anal function and reduce the incidence of adverse events during the waiting period for surgery.