Objective:To evaluate the effectiveness of a patient-family-centered visiting model in improving caregivers'beliefs and attitudes toward visiting patients with mental disorders.Methods:A total of 140 caregivers of inpatients diagnosed with mental disorders according to the ICD-10 were randomly assigned to an intervention group(n=71)and a control group(n=69).The intervention group participated a patient-family-centered visiting model for 4 weeks,while the control group followed routine visitation.Caregivers' beliefs and attitudes were assessed using the Beliefs and Attitudes toward Visitation in ICU Questionnaire(BAVIQ)was used to assess the improvement in caregivers' beliefs and attitudes before the intervention and at the end of the 1st,2nd,3rd,and 4th weeks after the intervention.Results:Repeated measures ANOVA showed that the belief score and attitude score of the BAVIQ questionnaire in the intervention group were significantly higher than those in the control group(Ps＜0.05)across time points.Chi-square test further confirmed that the improvement in the intervention group were significantly bet-ter than in the control group(Ps＜0.05).Conclusion:The patient-family-centered visiting model is more effective than routine visiting model in improving the caregivers' visiting beliefs and attitudes in mental health settings.

Liver macrophages are crucial components of innate immune system in liver and play a pivotal role in chronic hepatitis B virus (HBV) infection. Influenced by the liver microenvironment, liver macrophages can exhibit diverse immunophenotypes and functions, which contribute to either inhibiting HBV infection or mediating immune tolerance. Additionally, HBV actively regulates the phenotype of liver macrophages, thereby facilitating continuous viral infection. Given that the interaction between liver macrophages and HBV might directly impact the outcome of HBV infection, the huge potential clinical value of targeting macrophage markers, this review highlights the research progress on liver macrophages in HBV infection from three aspects: the origin and heterogeneity of liver macrophages, the interaction between liver macrophages and HBV, and the potential serological immune markers associated with macrophages in HBV infection.

Objective:To evaluate the effectiveness of a patient-family-centered visiting model in improving caregivers'beliefs and attitudes toward visiting patients with mental disorders.Methods:A total of 140 caregivers of inpatients diagnosed with mental disorders according to the ICD-10 were randomly assigned to an intervention group(n=71)and a control group(n=69).The intervention group participated a patient-family-centered visiting model for 4 weeks,while the control group followed routine visitation.Caregivers' beliefs and attitudes were assessed using the Beliefs and Attitudes toward Visitation in ICU Questionnaire(BAVIQ)was used to assess the improvement in caregivers' beliefs and attitudes before the intervention and at the end of the 1st,2nd,3rd,and 4th weeks after the intervention.Results:Repeated measures ANOVA showed that the belief score and attitude score of the BAVIQ questionnaire in the intervention group were significantly higher than those in the control group(Ps＜0.05)across time points.Chi-square test further confirmed that the improvement in the intervention group were significantly bet-ter than in the control group(Ps＜0.05).Conclusion:The patient-family-centered visiting model is more effective than routine visiting model in improving the caregivers' visiting beliefs and attitudes in mental health settings.

Liver macrophages are crucial components of innate immune system in liver and play a pivotal role in chronic hepatitis B virus (HBV) infection. Influenced by the liver microenvironment, liver macrophages can exhibit diverse immunophenotypes and functions, which contribute to either inhibiting HBV infection or mediating immune tolerance. Additionally, HBV actively regulates the phenotype of liver macrophages, thereby facilitating continuous viral infection. Given that the interaction between liver macrophages and HBV might directly impact the outcome of HBV infection, the huge potential clinical value of targeting macrophage markers, this review highlights the research progress on liver macrophages in HBV infection from three aspects: the origin and heterogeneity of liver macrophages, the interaction between liver macrophages and HBV, and the potential serological immune markers associated with macrophages in HBV infection.

Objective:To investigate the correlation between plasma total α-synuclein (α-syn), phosphorylated α-synuclein (p-α-syn), neurofilament light chain(NfL) and subtypes of Parkinson disease(PD).Methods:A total of 62 PD patients admitted to the Department of Neurology, the Affiliated Huai 'an No. 1 People 's Hospital of Nanjing Medical University from September 2021 to January 2023 were selected and scored on the Hoehn-Yahr stage(H-Y), unified Parkinson's disease rating scale Ⅲ(UPDRS-Ⅲ), levodopa equivalent daily dosage(LEDD), mini-mental state examination(MMSE), Parkinson disease quality of life questionnaire(PDQ-39) and activities of daily living(ADL). During the same period, 25 healthy individuals matched in age and sex were enrolled as the control group (HC). Clinical characteristics and blood samples were collected. The plasma levels of α-syn, p-α-syn and NfL were detected by enzyme-linked immunosorbent assay(ELISA). PD patients were divided into tremor dominant type (TD, n=27) and akinetic-rigid dominant type (AR, n=35) based on UPDRS-Ⅲ scores. Multifactorial Logistic regression analysis was performed by SPSS 25.0 software to determine the influencing factors of subtypes of Parkinson disease. The receiver operating characteristic (ROC) curve was used to determine the optimal cut-off point of plasma NfL between the AR type and the TD type. Results:Plasma α-syn, p-α-syn, NfL levels in the PD group were significantly higher than those of the HC group ( Z=-2.537, -6.580, -7.101, all P<0.05). There were statistically significant differences between the AR type and the TD type in disease duration, H-Y stage, UPDRS-Ⅲ scores, LEDD and MMSE scores ( Z=-2.503, -3.021, -2.025, -2.086, -2.409, all P<0.05). While no significant difference was found in plasma α-syn and p-α-syn levels between the AR type and TD type ( Z=-0.341, -0.085, both P>0.05), the plasma NfL levels were notably higher in the AR type(92.79(16.84, 117.53) pg/mL) compared to the TD type (12.10(6.99, 100.17) pg/mL)( Z=-2.236, P<0.05). Plasma NfL levels were correlated with rigidity scores ( r=0.438, P<0.001), UPDRS-Ⅲ scores ( r=0.337, P<0.05) and motor subtypes ( r=0.286, P<0.05) in PD patients. Multivariate Logistic regression analysis showed that NfL was risk factor for AR( B=0.002, OR=1.002, 95% CI=1.001-1.003, P=0.017). The ROC curve analysis indicated that plasma NfL levels could predict different subtypes of PD with an AUC=0.667, optimal cutoff =26.527. Conclusion:There is a correlation between elevated plasma NfL levels and the occurrence of AR type of PD, suggesting that nerve injury is probably involved in the occurrence and progression of various motor subtypes of PD.

Objective:To evaluate the efficacy and safety of mitoxantrone hydrochloride liposome injection in the treatment of peripheral T-cell lymphoma (PTCL) in a real-world setting.Methods:This was a real-world ambispective cohort study (MOMENT study) (Chinese clinical trial registry number: ChiCTR2200062067). Clinical data were collected from 198 patients who received mitoxantrone hydrochloride liposome injection as monotherapy or combination therapy at 37 hospitals from January 2022 to January 2023, including 166 patients in the retrospective cohort and 32 patients in the prospective cohort; 10 patients in the treatment-na?ve group and 188 patients in the relapsed/refractory group. Clinical characteristics, efficacy and adverse events were summarized, and the overall survival (OS) and progression-free survival (PFS) were analyzed.Results:All 198 patients were treated with mitoxantrone hydrochloride liposome injection for a median of 3 cycles (range 1-7 cycles); 28 cases were treated with mitoxantrone hydrochloride liposome injection as monotherapy, and 170 cases were treated with the combination regimen. Among 188 relapsed/refractory patients, 45 cases (23.9%) were in complete remission (CR), 82 cases (43.6%) were in partial remission (PR), and 28 cases (14.9%) were in disease stabilization (SD), and 33 cases (17.6%) were in disease progression (PD), with an objective remission rate (ORR) of 67.6% (127/188). Among 10 treatment-na?ve patients, 4 cases (40.0%) were in CR, 5 cases (50.0%) were in PR, and 1 case (10.0%) was in PD, with an ORR of 90.0% (9/10). The median follow-up time was 2.9 months (95% CI 2.4-3.7 months), and the median PFS and OS of patients in relapsed/refractory and treatment-na?ve groups were not reached. In relapsed/refractory patients, the difference in ORR between patients with different number of treatment lines of mitoxantrone hydrochloride liposome injection [ORR of the second-line, the third-line and ≥the forth-line treatment was 74.4% (67/90), 73.9% (34/46) and 50.0% (26/52)] was statistically significant ( P = 0.008). Of the 198 PTCL patients, 182 cases (91.9%) experienced at least 1 time of treatment-related adverse events, and the incidence rate of ≥grade 3 adverse events was 66.7% (132/198), which was mainly characterized by hematologic adverse events. The ≥ grade 3 hematologic adverse events mainly included decreased lymphocyte count, decreased neutrophil count, decreased white blood cell count, and anemia; non-hematologic adverse events were mostly grade 1-2, mainly including pigmentation disorders and upper respiratory tract infection. Conclusions:The use of mitoxantrone hydrochloride liposome injection-containing regimen in the treatment of PTCL has definite efficacy and is well tolerated, and it is a new therapeutic option for PTCL patients.

Objective To explore the predictive value of INR in early stage of warfarin therapy (early INR)for anticoagulation intensity after 7 days of treatment. Methods The medical records of patients hospitalized in the Department of Cardiology,Linyi People′s Hospital,Shandong University from January 2012 to May 2015 were collected,who received warfarin anticoagulation therapy and underwent INR tests in the morning after 3 or 7 days of medication. The early INR meant INR after 3 days of warfarin treatment. According to INR after 7 days of warfarin treatment,the patients were divided into 2 groups, anticoagulation up to standard(INR 2. 0﹣3. 0)group and over﹣anticoagulation(INR﹥3. 0)group. The best critical value of early INR for predicting INR after 7 days of warfarin treatment was obtained by plotting ROC curve. The risk of over﹣anticoagulation after 7 days of warfarin treatment was compared in patients with early INR ≥critical value and﹤critical value. Univariate analysis was used to compare the clinical characteristics in the 2 groups. The indexes with P ﹤0. 100 were used as covariate and multivariate logistic regression analysis was performed. The odds ratio( OR)and its 95% confidence interval( CI)was calculated and independent risk factors of INR ﹥3. 0 after warfarin treatment were screened. Results A total of 75 patients with atrial fibrillation were enrolled in the study,including 38 males and 37 females,aged(64 ± 9),42 patients in the anticoagulation up to standard group and 33 patients in the over﹣anticoagulation group. There were significant differences in body weight,INR after 3 days of medication,and the number of patients with hypoproteinemia between the 2 groups(all P﹤0. 05),but no significant differences in other indicators (all P﹥0. 05). The results of ROC curve showed that the best critical value of anticoagulation intensity predicted by early INR was 1. 67,the area under the curve was 0. 915[95% CI:0. 828﹣0. 967],and the sensitivity and specificity were 0. 95 and 0. 82,respectively. The risk of over﹣anticoagulation in patients with 7 days of warfarin treatment in the group with early INR ≥1. 67 was significantly higher than that in the group with early INR ﹤1. 67[90. 0%(27/30)vs. 13. 3%(6/45),χ2 ＝39. 883,OR＝58. 50,95% CI:13. 45﹣254. 48,P﹤0. 001]. Multivariate logistic regression analysis showed that early INR≥1. 67 was an independent risk factor for over﹣anticoagulation after 7 days of treatment( OR＝48. 719,95% CI:10. 891﹣217. 940,P﹤0. 001). Conclusions The early INR can predict anticoagulation intensity after 7 days of treatment. Early INR≥1. 67 is an independent risk factor for over﹣anticoagulation after 7 days of warfarin treatment.

Objective To explore the predictive value of INR in early stage of warfarin therapy (early INR)for anticoagulation intensity after 7 days of treatment. Methods The medical records of patients hospitalized in the Department of Cardiology,Linyi People′s Hospital,Shandong University from January 2012 to May 2015 were collected,who received warfarin anticoagulation therapy and underwent INR tests in the morning after 3 or 7 days of medication. The early INR meant INR after 3 days of warfarin treatment. According to INR after 7 days of warfarin treatment,the patients were divided into 2 groups, anticoagulation up to standard(INR 2. 0﹣3. 0)group and over﹣anticoagulation(INR﹥3. 0)group. The best critical value of early INR for predicting INR after 7 days of warfarin treatment was obtained by plotting ROC curve. The risk of over﹣anticoagulation after 7 days of warfarin treatment was compared in patients with early INR ≥critical value and﹤critical value. Univariate analysis was used to compare the clinical characteristics in the 2 groups. The indexes with P ﹤0. 100 were used as covariate and multivariate logistic regression analysis was performed. The odds ratio( OR)and its 95% confidence interval( CI)was calculated and independent risk factors of INR ﹥3. 0 after warfarin treatment were screened. Results A total of 75 patients with atrial fibrillation were enrolled in the study,including 38 males and 37 females,aged(64 ± 9),42 patients in the anticoagulation up to standard group and 33 patients in the over﹣anticoagulation group. There were significant differences in body weight,INR after 3 days of medication,and the number of patients with hypoproteinemia between the 2 groups(all P﹤0. 05),but no significant differences in other indicators (all P﹥0. 05). The results of ROC curve showed that the best critical value of anticoagulation intensity predicted by early INR was 1. 67,the area under the curve was 0. 915[95% CI:0. 828﹣0. 967],and the sensitivity and specificity were 0. 95 and 0. 82,respectively. The risk of over﹣anticoagulation in patients with 7 days of warfarin treatment in the group with early INR ≥1. 67 was significantly higher than that in the group with early INR ﹤1. 67[90. 0%(27/30)vs. 13. 3%(6/45),χ2 ＝39. 883,OR＝58. 50,95% CI:13. 45﹣254. 48,P﹤0. 001]. Multivariate logistic regression analysis showed that early INR≥1. 67 was an independent risk factor for over﹣anticoagulation after 7 days of treatment( OR＝48. 719,95% CI:10. 891﹣217. 940,P﹤0. 001). Conclusions The early INR can predict anticoagulation intensity after 7 days of treatment. Early INR≥1. 67 is an independent risk factor for over﹣anticoagulation after 7 days of warfarin treatment.

Objective To analyze the variation tendency of correlative test indexes in the patients with type 2 diabetic mellitus (T2DM) and diabetic nephropathy (DN) .Methods The urinary microalbumin (M‐Alb) ,blood glucose and renal function were de‐termined in 167 cases of T2DM .Then the cases were divided into the simple DM group ,early DN group and clinical DN group ac‐cording to the excretion rates of urinary M‐Alb .The determined results were compared among 3 group and analyzed .Results The age in the simple DM group was smaller than that in the early DN group and clinical DN group (P<0 .05) .The positive rates of u‐rinary M‐Alb and urinary protein were highest in the clinical DN group ,the differences among 3 groups had statistical significance (P<0 .05) .The difference of blood glucose indexes had no statistical difference between the simple DM group and early DN group , the clinical DN group was apparently higher than that in the simple DM group and early DN group (P<0 .05) .The levels of urea (Urea) and creatinine(Cr) in the simple DM group were significantly lower than those in the early DN group and clinical DN group ,the differences among 3 groups had statistical significance (P<0 .01) .Conclusion The severe the DN ,the higher the urina‐ry M‐Alb ,blood glucose and renal function indicators ,the higher the positive rates of urinary protein and urinary glucose ,the more significant the tendency of renal function damage .

Objective To explore the inhibitory effect of 24 pseudomonas aeruginosa(PA) on pathogenic fungi ,such as candida albicans ,candida tropicalis ,candida glabrata ,candida parapsilosis ,candida krusei ,mucous spore bacterium (MSB) etc .Methods 24 PA isolates were collected from clinical specimens and identified by Gram′s stain ,oxidase production and the API 20NE system(bi-oMerieux ,France) .Cross-streaking method and sterilizing filter paper-disk method and co-cultured method were applied to observe the inhibitory effect of PA .Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analyzed the difference of bacte-rial proteins of PA .Results The results showed that some strains of 24 PA had strong inhibitory effect against pathogenic fungi , some strain had partial effect and others had no effect .Co-cultured test showed that PA could inhibit the growth of fungal hyphae . SDS-PAGE displayed the significant difference in secretive proteins between the PA strains which had strong effect and no effect . Conclusion PA have inhibitory effect upon common pathogenic fungi and and this might be related to inhibit fungal hyphae forma-tion ,various protein secretion and inhibit the growth of fungi .