ObjectiveTo develop traditional Chinese medicine （TCM） formulae for the treatment of nonsevere coronavirus disease 2019 （COVID-19） and to explore its anti-inflammatory mechanism. MethodsThe dysregulated signaling pathways were determined in macrophages from bronchoalveolar lavage fluid of COVID-19 patients and in lung epithelial cells infected with SARS-CoV-2 in vitro based on transcriptome analysis. A total of 102 TCM formulae for the clinical treatment of nonsevere COVID-19 were collected through literature. The pathway-reversing rates of these formulae in macrophages and lung epithelial cells were evaluated based on signature signaling pathways， and the basic formula was determined in conjunction with TCM theory. The commonly used Chinese materia medica for nonsevere COVID-19 were summarized from the 102 TCM formulae as abovementioned. And together with the screening results from the Pharmacopoeia of the People's Republic of China， a “Chinese materia medica pool” was esta-blished for the development of TCM formulae for COVID-19. The regulatory effects of each herb on signaling pathways were obtained based on targeted transcriptome analysis. Oriented at reversing dysregulated signaling pathways of COVID-19， the calculation was carried out， and the artificial intelligent methods for compositing formulae， that are exhaustive method and parallel computing， were used to obtain candidate compound formulas. Finally， with reference to professional experience， an innovative formula for the treatment of nonsevere COVID-19 was developed. The ethanol extract of the formula was evaluated for its anti-inflammatory effects by detecting the mRNA expression of interleukin 1b （Il1b）， C-X-C motif chemokine ligand 2 （Cxcl2）， C-X-C motif chemokine ligand 10 （Cxcl10）， C-C motif chemokine ligand 2 （Ccl2）， nitric oxide synthase 2 （Nos2）， and prostaglandin-endoperoxide synthase 2 （Ptgs2） using reverse transcription-quantitative polymerase chain reaction （RT-qPCR） in RAW264.7 cells treated with lipopolysaccharide （LPS）. ResultsIn macrophages and lung epithelial cells， 34 dysregulated signaling pathways associated with COVID-19 were identified respectively. The effects of the 102 formulae for clinical treatment of nonsevere COVID-19 were evaluated based on the dysregulated signaling pathways and targeted transcriptome， and the result showed that Yinqiao Powder and Pingwei Powder （银翘散合平胃散， YQPWP） ranked first， reversing 91.18% of the dysregulated signaling pathways in macrophages and 100% of the dysregulated signaling pathways in lung epithelial cells. Additionally， YQPWP had the function of scattering wind and clearing heat， resolving toxins and removing dampness in accordance with the pathogenesis of wind-heat with dampness in COVID-19. It was selected as the basic formula， and was further modified and optimized to develop an innovative fomula Qiaobang Zhupi Yin （翘蒡术皮饮， QBZPY） based on expert experience and artificial intelligence in composing formulae. QBZPY can reverse all the dysregulated signaling pathways associated with COVID-19 in macrophages and lung epithelial cells， with the reversing rates of 100%. The chief medicinal of QBZPY， including Lianqiao （Fructus Forsythiae）， Xixiancao （Herba Siegesbeckiae） and Niubangzi （Fructus Arctii）， can down-regulate multiple signaling pathways related with virus infection， immune response， and epithelial damage. RT-qPCR results indicated that compared with the model group， the QBZPY group down-regulated the mRNA expression of Il1b， tumor necrosis factor （Tnf）， Cxcl2， Cxcl10， Ccl2， Nos2 and Ptgs2 induced by LPS in RAW264.7 cells （P＜0.05 or P＜0.01）. ConclusionBased on targeted transcriptome analysis， expert experience in TCM and artificial intelligence， QBZPY has been developed for the treatment of nonsevere COVID-19. The ethanol extract of QBZPY has been found to inhibit mRNA expression of several pro-inflammatory genes in a cellular inflammation model.

Objective: To analyze the impact of parental sports concept and behavior on physical activity in preschool children, so as to provide a foundation for future guidance on fostering childrens physical activity within the family context. Methods: From November to December 2020, a clustered convenience sampling method was employed to conduct surveys, and a total of 283 children were selectal from one kindergarten each in Beijing, Shenyang, and Xian. Participating children wore ActiGraph GT9X accelerometers continuously for one week to collect data on different intensity levels of physical activity. Physical Activity afterschool Questionnaire for Preschooler (P-PAQ) was utilized to assess parental sports concept and behavior. The gender differences in physical activity level and physical activity compliance rate were analyzed by using ttest, Mann-Whitney U test, and Chisquare test; and the relationship between parental exercise concepts and behaviors and physical activity of preschool children was analyzed using Spearman rank correlation analysis and multiple linear regression analysis. Results: Parental sports concept was significantly positively correlated with average daily moderatetovigorous physical activity (MVPA) and total physical activity (TPA) in children (r=0.12-0.16, P<0.05). Parental sports behavior was significantly positively correlated with childrens average daily TPA (r=0.25, P<0.05). Multiple linear regression revealed that parental sports concept was positively correlated with average daily MVPA and TPA in both boys and girls (B=0.65-0.83), while parental sports behavior only was positively correlated with boys average daily MVPA and TPA (B=0.24-0.25)(P<0.05). Conclusions Parental sports concept and behavior can impact physical activity levels in preschool children, exhibiting gender differences. Future guidance on physical activity in family upbringing should consider both parental sports concept and behavior, and pay attention to the influence of childrens gender.

ObjectiveTo explore the law of women reproductive aging based on theory of "seven-year period" in traditional Chinese medicine（TCM） through analyzing the characteristics of basic sex hormone levels and anti-müllerian hormone levels in women of different ages. MethodsThe data of female who visited Guangdong Provincial Hospital of Traditional Chinese Medicine from January 2018 to December 2022 and accepted basic hormone and anti-müllerian hormone determination were collected retrospectively. According to the age of subjects， they were divided into the "1-2 seven" group （under 21 years old）， "3 seven" group （21-27 years old）， "4 seven" group （28-34 years old）， "5 seven" group （35-41 years old）， "6 seven" group （42-48 years old） and "7 seven" and older group （49 years old and above）. The age， average menstrual cycle in the past 3 months， basic hormone levels including follicle stimulating hormone （FSH）， luteinizing hormone （LH）， estradiol （E₂）， testosterone （T） and FSH/LH value， and anti-müllerian hormone （AMH） levels were collected and compared. ResultsA total of 1221 cases were included. The differences in FSH， LH， FSH/LH values， E₂， T， AMH levels and average menstrual cycle among the groups were statistically significant （P＜0.05 or P＜0.01）. AMH peaked in the "3 seven" group. Compared to those in the "3 seven" group， the FSH/LH value in the "4 seven" group increased， while the LH， AMH and T levels decreased， and the menstrual cycle was shortened （P＜0.01）. The FSH/LH， LH， AMH， T and menstrual cycle in the "5 seven" group were consistent with the changing trend of those in the "4 seven" group， while the FSH and E₂ levels in the "5 seven" group were significantly higher （P＜0.05 or P＜0.01）. Compared to those in the "5 seven" group， the FSH， LH and E₂ levels of the "6 seven" group increased， and the AMH and T levels continued to decrease （P＜0.05 or P＜0.01）. Compared to those in the "6 seven" group， the FSH and LH levels of the "7 seven" and older group continued to increase， and the AMH and E₂ levels decreased （P＜0.01）. ConclusionThe law of women reproductive aging in the "seven-year period" theory is basically consistent with the characteristics of basic sex hormone levels and anti-müllerian hormone levels in women of different ages. The reproductive capacity reaches its peak in the "three-seven-year period" and gradually moves towards reproductive aging in the "four-seven-year period".

Objective:To evaluate the self-assessed and proxy-assessed quality of life among disabled elderly individuals aged 60 and above in Chengdu,analyze the factors influencing these assessments,and assess the consistency between these two types of outcomes and the feasibility of proxy assessments.Methods:The quality of life for disabled elderly individuals is measured using the EQ-5D-5L for both self-assessments and proxy assessments.Consistency testing is conducted using weighted Kappa and Intraclass Correlation Coefficient(ICC).Univariate and differential analyses are performed with the Mann-Whitney/Wilcoxon and Kruskal-Wallis tests,respectively.The analysis of influencing factors employs generalized linear model regression.Results:The results of proxy assessments are slightly higher than those of self-assessments.The consistency between self-assessed and proxy-assessed health utility values and EQ-VAS scores is good(with ICC values exceeding 0.7),while the consistency across various dimensions is fair to moderate(with weighted Kappa values approximately between 0.4 and 0.5).The degree of disability,economic status,outdoor activities and sleep,the relationship between caregivers and the elderly,and whether caregivers possess relevant caregiving knowledge,all have a statistically significant impact on the quality of life of disabled elderly individuals and the differences between self-assessed and proxy-assessed outcomes(P＜0.05).Conclusion:The quality of life of disabled elderly individuals is significantly lower than that of the general population.It is essential to consider various related factors to enhance the quality of life for these individuals.The EQ-5D-5L proxy assessment results from their primary caregivers can serve as a supplement to the self-assessment results of disabled elderly individuals.However,individual dimensions should be applied with caution,taking into account the characteristics and individual differences of the evaluated population,types of proxies,and measurement tools used.

Objective:To evaluate the self-assessed and proxy-assessed quality of life among disabled elderly individuals aged 60 and above in Chengdu,analyze the factors influencing these assessments,and assess the consistency between these two types of outcomes and the feasibility of proxy assessments.Methods:The quality of life for disabled elderly individuals is measured using the EQ-5D-5L for both self-assessments and proxy assessments.Consistency testing is conducted using weighted Kappa and Intraclass Correlation Coefficient(ICC).Univariate and differential analyses are performed with the Mann-Whitney/Wilcoxon and Kruskal-Wallis tests,respectively.The analysis of influencing factors employs generalized linear model regression.Results:The results of proxy assessments are slightly higher than those of self-assessments.The consistency between self-assessed and proxy-assessed health utility values and EQ-VAS scores is good(with ICC values exceeding 0.7),while the consistency across various dimensions is fair to moderate(with weighted Kappa values approximately between 0.4 and 0.5).The degree of disability,economic status,outdoor activities and sleep,the relationship between caregivers and the elderly,and whether caregivers possess relevant caregiving knowledge,all have a statistically significant impact on the quality of life of disabled elderly individuals and the differences between self-assessed and proxy-assessed outcomes(P＜0.05).Conclusion:The quality of life of disabled elderly individuals is significantly lower than that of the general population.It is essential to consider various related factors to enhance the quality of life for these individuals.The EQ-5D-5L proxy assessment results from their primary caregivers can serve as a supplement to the self-assessment results of disabled elderly individuals.However,individual dimensions should be applied with caution,taking into account the characteristics and individual differences of the evaluated population,types of proxies,and measurement tools used.

Objective:To evaluate the self-assessed and proxy-assessed quality of life among disabled elderly individuals aged 60 and above in Chengdu,analyze the factors influencing these assessments,and assess the consistency between these two types of outcomes and the feasibility of proxy assessments.Methods:The quality of life for disabled elderly individuals is measured using the EQ-5D-5L for both self-assessments and proxy assessments.Consistency testing is conducted using weighted Kappa and Intraclass Correlation Coefficient(ICC).Univariate and differential analyses are performed with the Mann-Whitney/Wilcoxon and Kruskal-Wallis tests,respectively.The analysis of influencing factors employs generalized linear model regression.Results:The results of proxy assessments are slightly higher than those of self-assessments.The consistency between self-assessed and proxy-assessed health utility values and EQ-VAS scores is good(with ICC values exceeding 0.7),while the consistency across various dimensions is fair to moderate(with weighted Kappa values approximately between 0.4 and 0.5).The degree of disability,economic status,outdoor activities and sleep,the relationship between caregivers and the elderly,and whether caregivers possess relevant caregiving knowledge,all have a statistically significant impact on the quality of life of disabled elderly individuals and the differences between self-assessed and proxy-assessed outcomes(P＜0.05).Conclusion:The quality of life of disabled elderly individuals is significantly lower than that of the general population.It is essential to consider various related factors to enhance the quality of life for these individuals.The EQ-5D-5L proxy assessment results from their primary caregivers can serve as a supplement to the self-assessment results of disabled elderly individuals.However,individual dimensions should be applied with caution,taking into account the characteristics and individual differences of the evaluated population,types of proxies,and measurement tools used.

Objective:To evaluate the self-assessed and proxy-assessed quality of life among disabled elderly individuals aged 60 and above in Chengdu,analyze the factors influencing these assessments,and assess the consistency between these two types of outcomes and the feasibility of proxy assessments.Methods:The quality of life for disabled elderly individuals is measured using the EQ-5D-5L for both self-assessments and proxy assessments.Consistency testing is conducted using weighted Kappa and Intraclass Correlation Coefficient(ICC).Univariate and differential analyses are performed with the Mann-Whitney/Wilcoxon and Kruskal-Wallis tests,respectively.The analysis of influencing factors employs generalized linear model regression.Results:The results of proxy assessments are slightly higher than those of self-assessments.The consistency between self-assessed and proxy-assessed health utility values and EQ-VAS scores is good(with ICC values exceeding 0.7),while the consistency across various dimensions is fair to moderate(with weighted Kappa values approximately between 0.4 and 0.5).The degree of disability,economic status,outdoor activities and sleep,the relationship between caregivers and the elderly,and whether caregivers possess relevant caregiving knowledge,all have a statistically significant impact on the quality of life of disabled elderly individuals and the differences between self-assessed and proxy-assessed outcomes(P＜0.05).Conclusion:The quality of life of disabled elderly individuals is significantly lower than that of the general population.It is essential to consider various related factors to enhance the quality of life for these individuals.The EQ-5D-5L proxy assessment results from their primary caregivers can serve as a supplement to the self-assessment results of disabled elderly individuals.However,individual dimensions should be applied with caution,taking into account the characteristics and individual differences of the evaluated population,types of proxies,and measurement tools used.

Objective:To evaluate the self-assessed and proxy-assessed quality of life among disabled elderly individuals aged 60 and above in Chengdu,analyze the factors influencing these assessments,and assess the consistency between these two types of outcomes and the feasibility of proxy assessments.Methods:The quality of life for disabled elderly individuals is measured using the EQ-5D-5L for both self-assessments and proxy assessments.Consistency testing is conducted using weighted Kappa and Intraclass Correlation Coefficient(ICC).Univariate and differential analyses are performed with the Mann-Whitney/Wilcoxon and Kruskal-Wallis tests,respectively.The analysis of influencing factors employs generalized linear model regression.Results:The results of proxy assessments are slightly higher than those of self-assessments.The consistency between self-assessed and proxy-assessed health utility values and EQ-VAS scores is good(with ICC values exceeding 0.7),while the consistency across various dimensions is fair to moderate(with weighted Kappa values approximately between 0.4 and 0.5).The degree of disability,economic status,outdoor activities and sleep,the relationship between caregivers and the elderly,and whether caregivers possess relevant caregiving knowledge,all have a statistically significant impact on the quality of life of disabled elderly individuals and the differences between self-assessed and proxy-assessed outcomes(P＜0.05).Conclusion:The quality of life of disabled elderly individuals is significantly lower than that of the general population.It is essential to consider various related factors to enhance the quality of life for these individuals.The EQ-5D-5L proxy assessment results from their primary caregivers can serve as a supplement to the self-assessment results of disabled elderly individuals.However,individual dimensions should be applied with caution,taking into account the characteristics and individual differences of the evaluated population,types of proxies,and measurement tools used.

Objective:To evaluate the self-assessed and proxy-assessed quality of life among disabled elderly individuals aged 60 and above in Chengdu,analyze the factors influencing these assessments,and assess the consistency between these two types of outcomes and the feasibility of proxy assessments.Methods:The quality of life for disabled elderly individuals is measured using the EQ-5D-5L for both self-assessments and proxy assessments.Consistency testing is conducted using weighted Kappa and Intraclass Correlation Coefficient(ICC).Univariate and differential analyses are performed with the Mann-Whitney/Wilcoxon and Kruskal-Wallis tests,respectively.The analysis of influencing factors employs generalized linear model regression.Results:The results of proxy assessments are slightly higher than those of self-assessments.The consistency between self-assessed and proxy-assessed health utility values and EQ-VAS scores is good(with ICC values exceeding 0.7),while the consistency across various dimensions is fair to moderate(with weighted Kappa values approximately between 0.4 and 0.5).The degree of disability,economic status,outdoor activities and sleep,the relationship between caregivers and the elderly,and whether caregivers possess relevant caregiving knowledge,all have a statistically significant impact on the quality of life of disabled elderly individuals and the differences between self-assessed and proxy-assessed outcomes(P＜0.05).Conclusion:The quality of life of disabled elderly individuals is significantly lower than that of the general population.It is essential to consider various related factors to enhance the quality of life for these individuals.The EQ-5D-5L proxy assessment results from their primary caregivers can serve as a supplement to the self-assessment results of disabled elderly individuals.However,individual dimensions should be applied with caution,taking into account the characteristics and individual differences of the evaluated population,types of proxies,and measurement tools used.

Objective:To evaluate the self-assessed and proxy-assessed quality of life among disabled elderly individuals aged 60 and above in Chengdu,analyze the factors influencing these assessments,and assess the consistency between these two types of outcomes and the feasibility of proxy assessments.Methods:The quality of life for disabled elderly individuals is measured using the EQ-5D-5L for both self-assessments and proxy assessments.Consistency testing is conducted using weighted Kappa and Intraclass Correlation Coefficient(ICC).Univariate and differential analyses are performed with the Mann-Whitney/Wilcoxon and Kruskal-Wallis tests,respectively.The analysis of influencing factors employs generalized linear model regression.Results:The results of proxy assessments are slightly higher than those of self-assessments.The consistency between self-assessed and proxy-assessed health utility values and EQ-VAS scores is good(with ICC values exceeding 0.7),while the consistency across various dimensions is fair to moderate(with weighted Kappa values approximately between 0.4 and 0.5).The degree of disability,economic status,outdoor activities and sleep,the relationship between caregivers and the elderly,and whether caregivers possess relevant caregiving knowledge,all have a statistically significant impact on the quality of life of disabled elderly individuals and the differences between self-assessed and proxy-assessed outcomes(P＜0.05).Conclusion:The quality of life of disabled elderly individuals is significantly lower than that of the general population.It is essential to consider various related factors to enhance the quality of life for these individuals.The EQ-5D-5L proxy assessment results from their primary caregivers can serve as a supplement to the self-assessment results of disabled elderly individuals.However,individual dimensions should be applied with caution,taking into account the characteristics and individual differences of the evaluated population,types of proxies,and measurement tools used.