Objective:To assess the efficacy and toxicities of carboplatin+etoposide(CE)regimens combined with abiraterone+prednisone(AAP)in patients with metastatic castration-resistant prostate cancer(mCRPC)after progression with docetaxel+prednisone(DP)regimens chemotherapy and novel hormone therapy(NHT).Methods:Retrospective analysis of mCRPC treated with DP regimens chemotherapy and/or NHT after progression,received CE regimens with AAP every 3 weeks for one cycle×6 cycles.The outcome were prostate specific an-tigen(PSA)response rate,time to PSA progression(TTPP),radiographic progression-free survival(rPFS),30%reduction in PSA,90%reduc-tion in PSA,the objective response remission rate and overall survival(OS).Results:From March 2019 to February 2024,37 eligible mCRPC patients were admitted to Cancer Hospital of Huanxing Chaoyang District Beijing and National Cancer Center/National Cancer Clinical Re-search Center/Cancer Hospital.After progression,CE regimens combined with AAP regimens was used for treatment.The median follow-up was 12.0(3.0-57.0)months.The median treatment cycle was 4 cycles.The PSA response rate was 42.1%.The median TTPP was 4.0 months;the median rPFS was 8.9 months and the median OS was 15.0 months.The objective remission rate was 24.3%,the proportion of 30%de-crease in PSA was 59.5%,and the proportion of 90%decrease in PSA was 16.2%.As for treatment side effects,10 cases had grade 3 or higher adverse reactions.Conclusions:CE regimens combined with AAP for mCRPC patients who failed DP regimens chemotherapy and/or NHT initially showed good clinical efficacy and tolerability.Additional sample size and follow-up time are needed to further validate the effic-acy.

Objective To compare the therapeutic effects of "three-endoscopy" and"two-endoscopy" respectively combined with traditional open surgery in the treatment of gallbladder stone complicated with common bile duct stone , and to explore the clinical value of "three-endoscopy" in the treatment of common bile duct stone complicated with gallbladder stone .Methods First, the pa-tients with upper abdominal surgical history and those patients with common bile duct diameter smaller than 0.6 cm and or those with stone diameter in common bile duct larger than 2.0 cm were screened and excluded from the study .The included patients with con-firmed gallbladder stone complicated with common bile duct stone were divided in accordance with different treatment methods into 4 groups:the traditional open surgery group (or group A), the Laparoscopy plus Bile duct endoscopy group (or group B), the Laparosco-py plus duodenal endoscopy group (or group C) and the"three-endoscopy" group (or group D), each consisting of 50 patients.Com-parisons were made in the success rate of surgery , residual stone rate , blood loss in the process of surgery , surgical time , drainage tube indwelling time , average air exhaust time after surgery , early ambulation time after surgery , cases of early complications and average hospitalization days between the 4 groups.Results There was no significant difference in the success rate of surgery between the"three-endoscopy" group and other treatment groups (P>0.05).The amount of blood loss in the process of surgery [(109.6 ±53.7) ml], surgical time [(117.3 ±53.2)h] and drainage tube indwelling time [(5.3 ±0.2)d] were significantly less than those of group A [(273.6 ±67.3)ml, (162.3 ±23.7)h, (33.6 ±5.3)d](P<0.05), Drainage tube indwelling time were significantly less than those of group A and group B (P<0.05), however, there were no significant differences, as compared with those of group C (P>0.05).In the "three-endoscopy" treatment group, residual stone rate, air exhaust time after surgery, ambulation time after surgery, cases of complications and average hospitalization days were significantly less than those of group A and group C (P >0.05). Conclusion When compared with traditional open surgery and "two-endoscopy" in the treatment of gallbladder stone combined with common bile duct stone , "three-endoscopy" therapy has the advantages of less trauma , less complications , faster recovery , shorter length of stay in hospital , with this reason , it is worth further clinical promotion .

Objective To prepare the immunoconjugate composed of Adriamycin nanoparticles and VEGFR 2 monoclonal antibody(conjugate of ADM-NP and VEGFR 2-MAb) and study its pharmacokinetics property. Methods Adriamycin nanoparticles were prepared by using double emulsion method, with PLA and O-CMC as materials. Conjugate of ADM-NP and VEGFR 2-MAb was prepared by using molecule conjugate technology. Immunoreactivity of the conjugate with type IV collagenase and H 22 cell were analyzed by using ELISA. Pharmacokinetics parameters of the immunoconjugate were obtained by using SD rats as study objects. Results The prepared ADM-NP was sphere particles under SEM, which diameters were (160±34) nm. The drug loading rate and entrapment rate were (30.15±3.5)% and (80.56±4.24)% respectively. Conjugate of ADM-NP and VEGFR 2-MAb was successfully prepared, which had immunoreactivity with type IV collagenase and H 22 cell. The immunoconjugate showed good ADM control-release ability and could prolong the retention time of ADM in vivo. Conclusion Conjugate of ADM-NP and VEGFR 2-MAb keeps the immunoreactivity of VEGFR 2-MAb and shows good ADM control-release ability.

Tissue factor is a powerful promoter of blood clotting in vivo , it plays an important role In hematischesis, tissue repair and thrombogenesis. In recent years, it is discovered that tissue factor can discriminate and regulate cell signal transduction, promote neogenesis of blood vessel and inflammatory reaction, regulate cell adhesion and movement, have a intimate relationship with invasion and metastasis of malignant tumor. In this article, we review the effects of tissue factor on malignant tumor metastasis.

Objective To study the influences by a Cyelin-dependent kinase inhibitor Roscovitine on cell cycle in mitotic hepatoma carcinoma cell SMMC-7721. Methods Microscope,MTT, flow cytometry and R-T PCR were used to observe the effects of Roscovitine on morphology, proliferation, cell cycle, apoptosis and the mRNA expression of CDK2, Caspase-3, bcl-2 in SMMC-7721 cells. Results Roscovitine inhibited the proliferation of SMMC-7721 cells in dosage and time dependent manner and induced apoptosis. Flow cytometry showed the ratio of G0, G1 increased. R-T PCR showed that the expression of bcl-2 reduced, Caspase-3 increased. Conclusion Reseovitine can inhibit the growth, proliferation, block the cell cycle at G0/G1 and promotes apoptosis of mitotic SMMC-7721 cells, and the mechanism of apoptosis is dependent on the activity of bcl-2 and Caspase-3.