Objective To investigate the effect of propofol on the secretory function of adrenal medullary chromaffin cells of rats.Methods The rat adrenal pheochromocytoma cells cultured in vitro were seeded in 24-well plates at a density of 1×1010 cells/ml and divided into 5 groups (n=12 each) using a random number table:control group (group C),different concentrations of propofol groups (P1 and P2 groups),and different concentrations of etomidate groups (E1 and E2 groups).Propofol was added with the final concentrations of 30 and 100 μmol/L in P1 and P2 groups,respectively.Etomidate was added with the final concentrations of 4 and 40 μmol/L in EI and E2 groups,respectively.The cells were then incubated for 10 min in an incubator at 37 ℃.At the end of incubation with drugs,6 wells in each group were selected,and physiological salt solution 200 μl was added;another 6 wells in each group were selected,and high K+ physiological salt solution was added.[3 H] PSS and [3 H] K+-PSS were measured by [3 H] norepinephrine release assays.Results Compared with group C,[3 H] K+-PSS and [3 H] K+-PSS/PSS were significantly decreased in P1,P2 and E2 groups (P＜0.05),and no significant changes were found in [3H] K+-PSS and [3H] K+-PSS/PSS in group E1 (P＞0.05).Compared with group P1,[3 H] K+-PSS and [3 H] K+-PSS/ PSS were significantly decreased in group P2 (P＜0.05).Conclusion Propofol can inhibit the secretory function of adrenal medullary chromaffin cells of rats in a concentration-dependent manner.

Objective To study the safety and efficacy of oral lactulose coupled with bifico as pre-surgical bowel preparation in patients with primary liver cancer .Methods With the knowledge and consent of the patients , a total of 80 patients were randomly divided into 2 groups, the experimental group and the control group , each consisting of 40 patients.The experimental group received o-ral lactulose plus bifico and the control group was given oral polyethylene glycol electrolytes as pre -surgical bowel preparation before sur-gery.Then, patients′tolerance, acceptance, adverse reactions, bowel cleansing and post-surgical bowel movement recovery were ob-served and compared between the 2 groups.Results No statistical significance in bowel cleansing could be observed , following imple-mentation of the drugs for the two groups (P>0.05).The texture and acceptance of the drugs for the experimental group were obviously superior to those of the control group, with statistical significance(P<0.05).With the exception of allergic reaction, no statistical difference could be noted in adverse reactions to pre-surgical bowel preparation for both groups (P>0.05).The incidence of other ad-verse reactions for the experimental group was lower than that of the control group , with statistical significance(P<0.05).Following pre-surgical bowel preparation , frequency of defecation and recovery of liver function for the experimental group were superior than those of the control group, also with statistical significance, when comparisons were made between the 2 groups (P<0.05).Conclusion Lactulose coupled with bifico as pre-surgical bowel preparation was safe and feasible , which could not only improve the sense of comfort for the patients, but increased their compliance , and decreased medical cost as well .