Objective:To explore the effects of nursing interventions based on the self-mutual-group (SMG) health management model on self-efficacy, medication adherence, and lower limb functional recovery in patients with hemiplegia after ischemic stroke.Methods:Convenience sampling was used to select 200 patients with hemiplegia after ischemic stroke admitted to the First Affiliated Hospital of Zhengzhou University from February 2022 to January 2024 as study subjects. Patients were randomly assigned to a control group and an SMG group, with 100 patients in each group. Control group received conventional rehabilitation nursing, while SMG group received nursing interventions based on the SMG health management model, with both groups receiving interventions for 12 weeks. Self-efficacy, medication adherence, and lower limb functional recovery between two groups were compared.Results:After 12 weeks of intervention, the Stroke Self-Efficacy Questionnaire score, Various Medication Adherence scores, Fugl-Meyer Assessment Lower Extremity score, step frequency, step speed, stride length ratio, and surface electromyography integral values of the tibialis anterior and gastrocnemius muscles of the affected limb were all higher than those before intervention, and those of SMG group were higher than those of control group. All of the above differences were statistically significant ( P<0.05) . Conclusions:Nursing interventions based on the SMG health management model can improve the self-efficacy and medication compliance of patients with hemiplegia after ischemic stroke, enhance lower limb motor function and walking ability, and promote changes in surface electromyographic signals of the tibialis anterior and gastrocnemius muscles.

Objective:To explore the effects of nursing interventions based on the self-mutual-group (SMG) health management model on self-efficacy, medication adherence, and lower limb functional recovery in patients with hemiplegia after ischemic stroke.Methods:Convenience sampling was used to select 200 patients with hemiplegia after ischemic stroke admitted to the First Affiliated Hospital of Zhengzhou University from February 2022 to January 2024 as study subjects. Patients were randomly assigned to a control group and an SMG group, with 100 patients in each group. Control group received conventional rehabilitation nursing, while SMG group received nursing interventions based on the SMG health management model, with both groups receiving interventions for 12 weeks. Self-efficacy, medication adherence, and lower limb functional recovery between two groups were compared.Results:After 12 weeks of intervention, the Stroke Self-Efficacy Questionnaire score, Various Medication Adherence scores, Fugl-Meyer Assessment Lower Extremity score, step frequency, step speed, stride length ratio, and surface electromyography integral values of the tibialis anterior and gastrocnemius muscles of the affected limb were all higher than those before intervention, and those of SMG group were higher than those of control group. All of the above differences were statistically significant ( P<0.05) . Conclusions:Nursing interventions based on the SMG health management model can improve the self-efficacy and medication compliance of patients with hemiplegia after ischemic stroke, enhance lower limb motor function and walking ability, and promote changes in surface electromyographic signals of the tibialis anterior and gastrocnemius muscles.

Objective:To explore the application of nursing intervention based on the capability, opportunity, motivation-behavior model (COM-B) in stroke patients during the rehabilitation period.Methods:Using the convenience sampling method, 146 stroke patients admitted to the First Affiliated Hospital of Zhengzhou University from February 2021 to February 2024 were selected as the research objects. According to the admission time sequence, they were divided into the COM-B group and the routine group, with 73 cases in each group. The routine group received routine nursing measures, and the COM-B group was intervened with the COM-B intervention, both for 3 months. The scores of the Health Education Self-Management Scale for Stroke Patients (HES-SP), the Exercise of Self-Care Agency Scale (ESCA), the Fugl-Meyer Assessment (FMA), and the Barthel Index of the two groups before and after the intervention were compared.Results:Finally, 72 patients in the COM-B group and 71 patients in the routine group completed the study. After 3 months of intervention, the scores of ESCA, HES-SP, FMA, and the Barthel Index of the two groups were all higher than those before the intervention, and the scores of COM-B group were all higher than those of the routine group, and the differences were statistically significant ( P<0.05) . Conclusions:The application of the COM-B intervention in stroke patients can improve patients' healthy behaviors and self-care abilities, and enhance their limb motor function and activities of daily living abilities, with a good application effect.

Objective:To explore the application of nursing intervention based on the capability, opportunity, motivation-behavior model (COM-B) in stroke patients during the rehabilitation period.Methods:Using the convenience sampling method, 146 stroke patients admitted to the First Affiliated Hospital of Zhengzhou University from February 2021 to February 2024 were selected as the research objects. According to the admission time sequence, they were divided into the COM-B group and the routine group, with 73 cases in each group. The routine group received routine nursing measures, and the COM-B group was intervened with the COM-B intervention, both for 3 months. The scores of the Health Education Self-Management Scale for Stroke Patients (HES-SP), the Exercise of Self-Care Agency Scale (ESCA), the Fugl-Meyer Assessment (FMA), and the Barthel Index of the two groups before and after the intervention were compared.Results:Finally, 72 patients in the COM-B group and 71 patients in the routine group completed the study. After 3 months of intervention, the scores of ESCA, HES-SP, FMA, and the Barthel Index of the two groups were all higher than those before the intervention, and the scores of COM-B group were all higher than those of the routine group, and the differences were statistically significant ( P<0.05) . Conclusions:The application of the COM-B intervention in stroke patients can improve patients' healthy behaviors and self-care abilities, and enhance their limb motor function and activities of daily living abilities, with a good application effect.

BRAF is one of the most important pro-oncogenes, which is mutated in approximately 8% of human tumors. The most common BRAF mutation is a valine-to-glutamate transition (V600E) that is expressed primarily in melanoma, colorectal cancer and thyroid carcinoma. MEK/ERK is constitutively activated in the cells expressing BRAFV600E, leading to tumor development, invasion, and metastasis. Therefore, BRAFV600E is a therapeutic target for melanoma and some other BRAFV600E tumors. Vemurafenib, a BRAFV600E inhibitor, which was approved by FDA for the treatment of late-stage melanoma in 2011, produces improved rates of overall and progression-free survival in patients with the BRAFV600E mutation, making a dramatic breakthrough in melanoma treatment. Vemurafenib is also an individual target drug based on genetic diagnosis. However, its therapeutic success is limited by the emergence of drug resistance. Therefore, it is important to explore the mechanisms underlying the resistance for developing new inhibitor drugs and for preventing or delaying the resistance evolution to BRAF inhibitor drugs. In this review, we described the role of BRAFV600E as an anti-tumor drug target and the development of BRAF inhibitors. We also discussed the mechanisms leading to resistance of BRAFV600E inhibitors. Furthermore, therapeutic strategies that might be employed to overcome acquired resistance were proposed.

Objective: To observe the effects of Tangnaikang (TNK), a compound traditional Chinese herbal medicine, on glucose metabolism and insulin resistance in obese Zucker rats. Methods: Twelve male obese Zucker rats, 6 weeks old, were randomly divided into control group and TNK group (3.24 g/kg) after being fed for 2 weeks. All rats received high-fat diet and 4-week treatment. Body weight and blood glucose were tested every week. Oral glucose tolerance test (OGTT) was performed and fasting insulin level was tested on days 0, 14 and 28. Triglyceride, cholesterol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and free fatty acids (FFA) were tested on day 28. Glucose infusion rate (GIR) was tested by hyperinsulinemic-euglycemic clamp from day 29. The protein expressions of protein kinase B (Akt), phospho-Akt (p-Akt) (Thr308) and glucose transporter protein 4 (GLUT4) in skeletal muscle and GLUT4 in adipose tissue were measured after hyperinsulinemic-euglycemic clamp test. Results: Compared with the control group, the fed blood glucose level and glucose level of OGTT at 120 min had a significant decline in TNK group on day 28, and TNK caused no alteration of the fasting serum insulin, and the GIR increased significantly in hyperinsulinemic-euglycemic clamp study. Furthermore, TNK increased Akt and p-Akt (Thr308) protein expressions in skeletal muscle and decreased the protein expression of GLUT4 in white adipose tissue. Body weight, and triglyceride, cholesterol, LDL-C and FFA contents were slightly decreased in the TNK group, but there were no statistically significant effects. Conclusion: TNK increases the protein expressions of Akt and p-Akt (Thr308) of the signal transduction pathway to influence the translocation of GLUT4 in skeletal muscle and improves glucose metabolism by reducing insulin resistance.

Objective To determine the content of cholesterol in Gekko Gecko.Methods High performance liquid chromatography was performed on Hypersil ODS2 (4.6 mm?150 mm,5 ?m).The chromatorgraphic conditions were as follows:methanol as mobile phase,flow rate being 1.0 mL/min and detecting wavelength at 208 nm.The column temperature was 40 ℃.Results A good linearity of cholesterol was shown in range of 0.885～14.000 ?g (r=0.999 5),the average recovery was 99.65%,RSD=3.45% (n=6).Conclusions This method is simple,rapid and accurate,and may provide a reliable way for the quantification of cholesterol in Gekko Gecko.