Objective To investigate the current status and influencing factors of anticipatory grief of main caregivers of young and middle-aged patients with advanced liver cancer by cross-sectional survey.Methods A total of 330 caregivers of young and middle-aged patients with advanced liver cancer who were admitted to The Third Affiliated Hospital of Naval Medical University from January 2023 to May 2023 were enrolled as research objects by convenience sampling.A questionnaire survey on general information,anticipatory grief and simple coping style was conducted on these caregivers one day before discharge.Results The score of anticipatory grief of the caregivers was 94.10±22.33.Multiple linear stepwise regression analysis was performed with statistically significant data from the characteristics of the research objects as independent variables and anticipatory grief as the dependent variable,and the result showed that gender,monthly income,educational level,personality self-assessment and coping were main factors affecting anticipatory grief of main caregivers of young and middle-aged patients with advanced liver cancer(all P<0.05).The mean score for positive response was 2.23±0.14,which was negatively associated with anticipatory grief(P=0.041).The mean score for negative response was 1.31±0.24,which was positively associated with anticipatory grief(P<0.001).Conclusion The anticipatory grief of main caregivers of young and middle-aged patients with advanced liver cancer is at a high level.The anticipatory grief is negatively related to positive coping,and positively related to negative coping.The main factors affecting anticipatory grief of main caregivers of young and middle-aged patients with advanced liver cancer are gender,family economic status,educational level,and introverted personality.

Objective To summarize the best evidence concerning the prevention and management of indwelling line complications in patients with hepatocellular carcinoma(HCC)receiving hepatic artery infusion chemotherapy(HAIC),and to standardize the key contents of clinical observation of complications during HAIC treatment.Methods By using the"6S"pyramid model system,the relevant literature was searched in the order from high to low.Two professionals evaluated the quality of the literature,summarized the evidence and conducted the analysis and summarization.Results Ten literature articles were finally enrolled in this study,including one article of guideline,one article of systematic review,five articles of expert consensus,one article of meta-analysis,and two articles of randomized controlled trials.Six complications(catheter displacement or falling off,catheter obstruction,unplanned extubation,arterial spasm or occlusion,infection,puncture site bleeding/local hematoma)and 22 pieces of best evidence for prevention management were summarized.Conclusion This study systematically summarizes 6 complications and their prevention and treatment in patients with HCC receiving HAIC,providing a reliable basis for clinical practice.

Objective To explore the effect of high-quality nursing on blood lipid and body mass index(BMI) in patients with chronic schizophrenia with metabolic syndrome (MS).Methods A total of 60 chronic schizophrenia cases combined with MS in our hospital were randomly divided into study group and control group.The control group was given routine nursing,while the study group was additional given high-quality nursing.Blood lipid and BMI were investigated at enrollment and 6 months after intervention.Results At 6 months after intervention,BMI,triglyceride (TG),high-density lipoprotein (HDL-C) levels in the study group were improved significantly than that at enrollment and the control group (P ＜0.01 or P ＜0.05),but there was no significant difference in the indexes in the control group and at enrollment (P ＞ 0.05).Conclusion High-quality nursing can improve the blood lipid and body mass index in patients with chronic schizophrenia with MS,and reduce the risk factors of MS.

Objective To explore the effect of high-quality nursing on blood lipid and body mass index(BMI) in patients with chronic schizophrenia with metabolic syndrome (MS).Methods A total of 60 chronic schizophrenia cases combined with MS in our hospital were randomly divided into study group and control group.The control group was given routine nursing,while the study group was additional given high-quality nursing.Blood lipid and BMI were investigated at enrollment and 6 months after intervention.Results At 6 months after intervention,BMI,triglyceride (TG),high-density lipoprotein (HDL-C) levels in the study group were improved significantly than that at enrollment and the control group (P ＜0.01 or P ＜0.05),but there was no significant difference in the indexes in the control group and at enrollment (P ＞ 0.05).Conclusion High-quality nursing can improve the blood lipid and body mass index in patients with chronic schizophrenia with MS,and reduce the risk factors of MS.

ObjectiveTo explore an appropriate way and its effect on neonatal resuscitation training in Uygur area of Xinjiang, China.MethodsFrom October 2014 to February 2015, obstetricians, neonatologists (pediatricians), midwives and anesthetists from Turpan Region Central Hospital, Turpan City People's Hospital,Aksu Region First People's Hospital and other five hospitals at county level were chosen to attend the training course of neonatal resuscitation. The textbook for the course was Guidelines for Resuscitation which had been translated to Uygur language from English and the class was lead by less than ten Uygur trainees with Mandarin and Uygur when necessary in addressing some difficult or important points. The duration of theoretic courses took 6 h and the operational course took 8 h. Theorectical exam was taken before, immediately after and three months after (before re-training) the training. The accuracy rate was applied to assess the effect of training through evaluation for each resuscitation techniques. Repeated measures analysis of variance andChi-square test were used for statistical analysis.ResultsA total of 220 health care staff were included in the study. The average score of theoretical examination after the training was higher than that before (85.68±8.52 vs 65.37±12.08,t=18.532,P=0.000), and that before re-training was lower than that after training (80.08±12.70 vs 85.68±8.52,t=-4.943,P=0.000). After the training, the proportions of Done in each item, including rapid assessment, preliminary resuscitation, correct positive pressure artificial ventilation, external cardiac massage together with artificial ventilation, proper endotracheal intubation and proper administration of resuscitation drugs, were all higher that those before [72.7%(160/220) vs 1.4%(3/220), 40.0%(88/220) vs 0%(0/220), 15.9%(35/220) vs 0%(0/220), 37.7%(83/220) vs 8.2%(18/220), 51.8%(114/220) vs 5.9%(13/220) and 48.2%(106/220) vs 10.5%(23/220), allP<0.01]. But at the time before re-training, only one proportion ofDone which was higher than those immediately after initial training was proper administration of resuscitation drugs [49.6%(109/220) vs 48.2%(106/220),χ2=9.129,P<0.05].ConclusionBilingual (Mandarin and Uygur) neonatal resuscitation training in Xinjiang minority areas might enhance the recovery skills for local medical personnel.

Objective To explore the application effects and evaluation of scenario pedagogy on hierarchical first aid training for nurses. Methods A total of 160 nursing staffs, who took part in the first aid training from July 2013 to June 2014, were selected and averagely divided into observation group ( scenario pedagogy) and control group ( traditional pedagogy) by random number table. We compared basic theory of first aid, operant skill of first aid and comprehensive ability. Results After the training, the theory, operant skill and comprehensive ability were (96. 25 ± 8. 31), (93. 64 ± 7. 48), (95. 24 ± 7. 65), that were all higher than those of the control group (t=3. 265, 2. 985, 2. 988;P<0. 01);in the observation group after the training, the N0, N1, N2 level of nursing staffs′comprehensive ability were (94. 41 ± 8. 22), (96. 24 ± 7. 43), (98. 21 ± 7. 46) higher than those of the control group (t=2. 982, 3. 041, 3. 215;P<0. 01). Conclusions Scenario pedagogy method can significantly mobilize the study enthusiasm of nurses at all levels, and increase all level nurses′basic emergency theory, emergency operation skills and comprehensive abilities.

Objective To explore the application value of dipstick dye immuno-assay (DDIA) for screening the schistosomiasis chemotherapy targets in the low endemic areas of Xiaogan City.Methods The residents aged 6-65 years in a village in the low endemic areas of schistosomiasis of Xiaogan City were selected and tested by the methods of fecal examination,DDIA,indirect hemagghitination (IHA),enzyme linked immunosorbent assay (ELISA) and inquiry,and the results of fecal examination were determined as the gold standard.Results The Youden' s indices of IHA,DDIA,ELISA and inquiry were 0.74,0.72,0.62 and 0.30,respectively,and the consistency rates of them were 93.38%,91.99%,81.53% and 70.03%,respectively.It took 16.70,4.95,4.12,5.63 and 2.44 Yuan screening one patient with the fecal examination,IHA,DDIA,ELISA and inquiry,respectively.Conclusion The validity of DDIA with simple operation and low cost for screening the schistosomiasis chemotherapy targets is satisfying,and the method is suitable for large scale screening in low endemic areas.

To obtain an ideal recombinant C-terminal fragment of the merozoite surface protein of Plasmodium falciparum in the Pichia pastoris expression system, the major surface protein-119 (MSP-119) gene sequence bearing the 6-his gene was inserted into expression vector pPIC9k and the target gene was transformed to the susceptible yeast cells GS115 by using electroporation. The multiple inserts were screened and the successfully expressed MSP-119 protein with the relative molecular weight of 12kDa in the supernatants of cell cultures could be detected by SDS-PAGE. Meanwhile, Western blot analysis also demonstrated that this protein reacted with mouse anti-MSP-119 monoclonal antibody, and the expression level of MSP-119 was more than 1.0 g/L. It is concluded that this recombinant protein expressed in the Pichia pastoris expression system resembles the native proteins existed.