Objective The aim of this study was to investigate the expression of nuclear pore membrane protein 121(POM121)in head and neck mucosal melanoma(HNMM)and its effect on the migration and invasion of HNMM cells.Methods The cancer tissues from 63 patients with HNMM and 20 adjacent normal oral epithelial tissues who were treated in the Harbin Cancer Hos-pital of Harbin Medical University from March 1,2011 to March 30,2015 were collected,and the expression of POM121 was detected by immunohistochemistry.The relationship between the expression of POM121 and the clinicopathological features of HNMM patients and its effect on the prognosis of patients were analyzed.Western blot was used to detect the expression of POM121 in HNMM cells(MM9H-1 cells)and human oral epithelial cells(human oral keratinocytes,HOK cells).After knocking down POM121 with small in-terfering RNA(siRNA),the effects on the migration and invasion of HNMM cells were detected by Scratch assay and Transwell assay.Results Immunohistochemistry showed that expression of POM121 was significantly higher in HNMM tissues than that in adjacent tissues(P<0.001).The expression of POM121 was associated with tumor size and stage(P<0.05).Kaplan-Meier analysis showed that the disease-free survival(DFS)and overall survival(OS)of patients with low expression of POM121 were significantly longer than those with high expression of POM121.Cox regression analysis showed that POM121 expression,AJCC stage and lymph node metasta-sis were influencing factors for OS and DFS of HNMM patients(P<0.05).Western blot results showed that the expression of POM121 protein in MM9H-1 cells was significantly higher than that in HOK cells(P<0.05).After knocking down POM121,the cell migration rate and cell invasion ability were significantly reduced(P<0.001).Conclusion POM121 is highly expressed in HNMM tissues and cells,and down-regulation of POM121 significantly inhibits the migration and invasion of HNMM cells.

Objective The aim of this study was to investigate the expression of nuclear pore membrane protein 121(POM121)in head and neck mucosal melanoma(HNMM)and its effect on the migration and invasion of HNMM cells.Methods The cancer tissues from 63 patients with HNMM and 20 adjacent normal oral epithelial tissues who were treated in the Harbin Cancer Hos-pital of Harbin Medical University from March 1,2011 to March 30,2015 were collected,and the expression of POM121 was detected by immunohistochemistry.The relationship between the expression of POM121 and the clinicopathological features of HNMM patients and its effect on the prognosis of patients were analyzed.Western blot was used to detect the expression of POM121 in HNMM cells(MM9H-1 cells)and human oral epithelial cells(human oral keratinocytes,HOK cells).After knocking down POM121 with small in-terfering RNA(siRNA),the effects on the migration and invasion of HNMM cells were detected by Scratch assay and Transwell assay.Results Immunohistochemistry showed that expression of POM121 was significantly higher in HNMM tissues than that in adjacent tissues(P<0.001).The expression of POM121 was associated with tumor size and stage(P<0.05).Kaplan-Meier analysis showed that the disease-free survival(DFS)and overall survival(OS)of patients with low expression of POM121 were significantly longer than those with high expression of POM121.Cox regression analysis showed that POM121 expression,AJCC stage and lymph node metasta-sis were influencing factors for OS and DFS of HNMM patients(P<0.05).Western blot results showed that the expression of POM121 protein in MM9H-1 cells was significantly higher than that in HOK cells(P<0.05).After knocking down POM121,the cell migration rate and cell invasion ability were significantly reduced(P<0.001).Conclusion POM121 is highly expressed in HNMM tissues and cells,and down-regulation of POM121 significantly inhibits the migration and invasion of HNMM cells.

Objective:To investigate the impact of diabetes mellitus (DM) and stroke on long-term outcomes in patients with acute coronary syndrome (ACS) undergoing percutaneous coronary intervention (PCI).Methods:Total 411 consecutive ACS patients undergoing PCI at the Ninth People′s Hospital of Zhengzhou between December 2014 and June 2018 were recruited, including 319 males and 92 females with a mean age of (64.7±10.1) years. These patients were divided into 4 groups according to the presence or absence of history of diabetes or stroke: non-DM non-stroke group ( n=192) , DM group ( n=140), stroke group ( n=41), and DM+stroke group ( n=38). The data of baseline demographic characteristics, clinical feature, coronary angiographic findings, and cardiovascular adverse events during long-term follow-up were obtained. Kaplan-Meier curves were used to investigate the long-term clinical outcomes among groups. Results:The mean interval of follow-up was (24.1±13.8) months. Patients with DM+stroke had the highest rates of non-fetal myocardial infarction (χ 2=24.932) , non-fetal stroke (χ 2=9.434) , hospitalization due to heart failure/angina (χ 2=69.290) , revascularization (χ 2=22.918) , cardiovascular death(χ 2=13.473)and all-cause death(χ 2=17.724)as well as hard endpoint events (the sum of non-fetal myocardial infarction, non-fetal stroke, and all-cause death) (χ 2=30.268)and combined major adverse cardiovascular events (MACE) (the sum of hard endpoint events, hospitalization due to heart failure/angina, and revascularization) (χ 2=119.556)among 4 groups(all P<0.01). In Kaplan-Meier survival analysis, the cumulative ratio of freedom from all-cause death decreased significantly in DM+stroke group compared with no DM no stroke group ( HR=17.121, 95 %CI: 2.527-115.934, P<0.01), but no statistical difference was observed in the cumulative ratio of freedom from all-cause death between DM+stroke group and DM group or stroke group respectively ( HR=3.178, 95 %CI: 0.744-13.582; HR=1.383, 95 %CI: 0.374-5.118; all P>0.05) . Meanwhile, patients with DM+stroke presented significantly lower cumulated ratio free from combined MACE than patients with non-DM non-stroke ( HR=5.423, 95 %CI:2.941-10.036, P<0.01), and the cumulated ratio free from combined MACE also decreased significantly in DM+stroke group as compared to DM group or stroke group respectively ( HR=1.859,95 %CI: 1.167-2.962; HR=1.991,95 %CI: 1.178-3.364; all P<0.01) . Conclusions:ACS patients with combined history of DM and stroke have a worse long-term outcomes after PCI than those with DM alone or stroke alone or without DM or stroke. DM and stroke seemed to have an additive effect on decrease in the cumulative ratio free from combined MACE in ACS patients following PCI.

Objective To determine the contributions of main chemical compositions of extracts of Eucalyptus camaldulensis represented by GC/MS elute peaks to the molluscicidal activities,and explore a shortcut of looking for the effective components from natural products. Methods E. camaldulensis leaves were collected consecutively in 12 months at the same place,extracted with dichloromethane,analyzed by GC/MS,and their LC50(s)of molluscicidal activities were tested according to the method rec-ommended by WHO. The correlation of the main components in 12 extracts and their molluscicidal activities were analyzed by the grey relative correlation analysis model with software GTMS 3.0. Result All the dichloromethane extracts of eucalyptus leaves showed excellent molluscicidal activities with the highest LC50 of 0.257mg/L and 0.242mg/L for the samples in June and July and the lowest LC50 of 6.802 mg/L and 5.406 mg/L in December and January respectively. The structures of 16 main chemical compo-nents were elucidated by GC/MS and NIST Mass Spectral Library,most of which were monoterpenes and sesquiterpenoids. The gray correlation coefficients with activity were all over 0.5,the first five over 0.9 were 4,4,8-Trimethyltricyclo[6.3.1.0(1,5)]do-decane-2,9-diol,(-)-Spathulenol,a structural isomer of(-)-Spathulenol,Eucalyptol and Ledol. Conclusion The most main in-gredients in the dichloromethane extracts of E. camaldulensis leaves show good correlations with the molluscicidal activity,which suggests that the molluscicidal role is synergistically played by the multiple components together.

Mebendazole is currently used in the treatment of hydatid disease.Its poor absorption from the gastro-intestinal tract and low bioavailability aroused further research on new formulations of mebendazole to increase the bioa-vailability and improve the therapeutic efficacy.This review summarizes the recent research progress.

Object To determine the content of muscone in preparation Methods Standard addition, DNP derivatization and HPLC with electrospray ionization tandem mass spectrometry (LC/ESI MS/MS) were utilized Results Identification of muscone hydrazone in the sample was based on the unique mass spectra of the standard Under an optimum condition peak corresponding to + ion and characteristic fragments for muscone hydrazone were observed ESI and APCI full scan m/z 419 MS/MS had the same relative molecular mass and fragmentation pattern The recovery was 102 5% with RSD ≤ 2 76% (n=5) Conclusion The qualitative and quantitative determination on muscone could be fulfilled simultaneously