Objective:To observe the effects of acupuncture on intestinal flora and inflammatory factor interleukin(IL)-6 in ulcerative colitis(UC)model rats and to explore the related mechanism of acupuncture in treating UC.Methods:Thirty-two Sprague-Dawley rats were randomly divided into a normal control(NC)group,a model(MO)group,a mesalazine(ME)group,and an acupuncture(AC)group,with 8 rats in each group.Rats,except those in the NC group,were given a 2.5%sodium dextran sulfate solution to establish UC models.After successful modeling,rats in the ME group were treated with mesalazine by gavage twice a day for 7 continuous days;rats in the AC group received acupuncture at bilateral Tianshu(ST25)and Zusanli(ST36)with needles retained for 20 min each time,once a day for 7 consecutive days.After intervention,changes in the intestinal flora diversity,colon tissue damage degree,and inflammatory factor expression in each group of rats were evaluated.Results:The body mass increased slowly in the MO group compared to the NC group.After intervention,the body mass increased more significantly,colon injuries recovered,and intestinal flora diversity improved in the ME and AC groups compared to the MO group(P<0.05).The IL-6 expression level was higher in the MO group compared to the NC group(P<0.05).The IL-6 expression level decreased in the AC group compared to the MO group(P<0.05).Conclusion:Acupuncture at Tianshu(ST25)and Zusanli(ST36)improves body mass loss and colon injuries,regulates intestinal flora diversity,and reduces the expression of inflammatory factor IL-6 in the colon tissue of UC rats,therefore alleviating the disease severity of UC.

Objective:To observe the effects of acupuncture on intestinal flora and inflammatory factor interleukin(IL)-6 in ulcerative colitis(UC)model rats and to explore the related mechanism of acupuncture in treating UC.Methods:Thirty-two Sprague-Dawley rats were randomly divided into a normal control(NC)group,a model(MO)group,a mesalazine(ME)group,and an acupuncture(AC)group,with 8 rats in each group.Rats,except those in the NC group,were given a 2.5%sodium dextran sulfate solution to establish UC models.After successful modeling,rats in the ME group were treated with mesalazine by gavage twice a day for 7 continuous days;rats in the AC group received acupuncture at bilateral Tianshu(ST25)and Zusanli(ST36)with needles retained for 20 min each time,once a day for 7 consecutive days.After intervention,changes in the intestinal flora diversity,colon tissue damage degree,and inflammatory factor expression in each group of rats were evaluated.Results:The body mass increased slowly in the MO group compared to the NC group.After intervention,the body mass increased more significantly,colon injuries recovered,and intestinal flora diversity improved in the ME and AC groups compared to the MO group(P<0.05).The IL-6 expression level was higher in the MO group compared to the NC group(P<0.05).The IL-6 expression level decreased in the AC group compared to the MO group(P<0.05).Conclusion:Acupuncture at Tianshu(ST25)and Zusanli(ST36)improves body mass loss and colon injuries,regulates intestinal flora diversity,and reduces the expression of inflammatory factor IL-6 in the colon tissue of UC rats,therefore alleviating the disease severity of UC.

Objective:To explore the efficacy of hyperbaric oxygen assisting clopidogrel, an antiplatelet drug, in the treatment of acute cerebral infarction (ACI) and its effect on platelet activation.Methods:A total of 144 ACI patients admitted to the Department of Neurology of Qingyang People’s Hospital from January 2017 to December 2018 were selected and randomly divided into control group and observation group with 72 cases in each group. The control group was treated with clopidogrel on the basis of conventional treatment, and the observation group was additionally treated with hyperbaric oxygen therapy on the basis of the treatment of the control group. The clinical efficacies of the two groups were compared. The scorings of Montreal cognitive assessment Scale (MoCA), mini-mental state examination (MMSE), and Barthel index were performed before and after treatment. Serum platelet activating factor (PAF) and platelet α-granule membrane glycoprotein (CD62P) were measured.Results:In the observation group, 33 patients were cured, 24 showed markedly effects, and 10 showed some improvements. The total effective rate (93.06%) in the observation group was significantly higher than that (84.72%) in the control group with a statistically significant difference ( P<0.05). After treatment, the scores of MoCA, MMSE, and Barthel index in the observation group were 25.83±1.73, 25.46±2.33, and 76.25±4.78, respectively, which were significantly higher than those in the control group (23.77±1.94, 21.85±2.48, and 65.36±5.12) ( P<0.05). In the two groups, the serum levels of PAF and CD62P after treatment were significantly lower than those before treatment, and the differences were statistically significant ( P<0.05), moreover, the reduction of serum levels of PAF and CD62P in the observation group were better than those in the control group, with statistically significant differences ( P<0.05). Conclusion:Hyperbaric oxygen assisting clopidogrel in the treatment of ACI can effectively reduce serum levels of PAF and CD62P, and reduce platelet activation to prevent thrombosis, thus the combined therapy can significantly improve the therapeutic effect of ACI and effectively restore the cognitive and physiological functions of patients, which is worthy of clinical promotion and application.

Objective:To explore the efficacy of hyperbaric oxygen assisting clopidogrel, an antiplatelet drug, in the treatment of acute cerebral infarction (ACI) and its effect on platelet activation.Methods:A total of 144 ACI patients admitted to the Department of Neurology of Qingyang People’s Hospital from January 2017 to December 2018 were selected and randomly divided into control group and observation group with 72 cases in each group. The control group was treated with clopidogrel on the basis of conventional treatment, and the observation group was additionally treated with hyperbaric oxygen therapy on the basis of the treatment of the control group. The clinical efficacies of the two groups were compared. The scorings of Montreal cognitive assessment Scale (MoCA), mini-mental state examination (MMSE), and Barthel index were performed before and after treatment. Serum platelet activating factor (PAF) and platelet α-granule membrane glycoprotein (CD62P) were measured.Results:In the observation group, 33 patients were cured, 24 showed markedly effects, and 10 showed some improvements. The total effective rate (93.06%) in the observation group was significantly higher than that (84.72%) in the control group with a statistically significant difference ( P<0.05). After treatment, the scores of MoCA, MMSE, and Barthel index in the observation group were 25.83±1.73, 25.46±2.33, and 76.25±4.78, respectively, which were significantly higher than those in the control group (23.77±1.94, 21.85±2.48, and 65.36±5.12) ( P<0.05). In the two groups, the serum levels of PAF and CD62P after treatment were significantly lower than those before treatment, and the differences were statistically significant ( P<0.05), moreover, the reduction of serum levels of PAF and CD62P in the observation group were better than those in the control group, with statistically significant differences ( P<0.05). Conclusion:Hyperbaric oxygen assisting clopidogrel in the treatment of ACI can effectively reduce serum levels of PAF and CD62P, and reduce platelet activation to prevent thrombosis, thus the combined therapy can significantly improve the therapeutic effect of ACI and effectively restore the cognitive and physiological functions of patients, which is worthy of clinical promotion and application.

Objective To study the therapeutic effects of Bioenterics Intragastric Balloon (BIB) on obesity under gastroscopy.Methods Data of 47 patients treated with Bioenterics Intragastric Balloon under gastrscopy were reviewed from July,2010 to May,2011.Results Weight loss ( mean 15.4 kg ) was successfully achieved in all the patients during 6 months.BMI decreased by 3.2-6.4 kg/m2 ( mean 4.7 kg/m2 ).There was no serious side effect with a better result for obesity according to the follow-up.Conclusion BIB is effective for obesity for noninvasiveness,stable speed of weight loss and less pain.

OBJECTIVETo study the effects and mechanisms of sinensetin on proliferation and apoptosis of human AGS gastric cancer cells.

METHODMTT assay was used to detect the growth inhibition rates of human AGS gastric cancer cells treated with sinsesectin in different concentrations and times. The cell cycle distribution was measured by flow cytometry. The apoptosis was examined by Annexin-FITC/PI staining and DNA fragment analysis. The apoptosis morphology was observed by inverted fluorescence microscope after Hoechst 33342 staining. The protein expressions of p21 and p53 were detected by western blot.

RESULTMTT assay showed that sinensetin inhibited the growth of AGS gastric cancer cells in a dose- and time-dependent manner. Sinensetin blocked AGS cells in G2/ M and increased the apoptosis rates of AGS cells in a dose-dependent manner. DNA ladder was observed in cells treated with 60 micromol x L(-1) sinensetin for 48 h. The typical apoptotic morphological changes including cell nucleus shrinkage, chromatin condensation and apoptotic bodies were observed when treated with different dose of sinensetin. Western blot showed that sinensetin increased expressions of p53 and p21 in a dose-dependent manner.

CONCLUSIONSinensetin could inhibit human AGS gastric cancer cells proliferation and induce cell cycle block in G2/M phase and apoptosis. The up regulation of p53 and p21 protein might be one of the mechanisms.

Apoptosis ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Cyclin-Dependent Kinase Inhibitor p21 ; analysis ; Dose-Response Relationship, Drug ; Flavonoids ; pharmacology ; Humans ; Stomach Neoplasms ; drug therapy ; pathology ; Tumor Suppressor Protein p53 ; analysis

Objective: The main ingredients and the inhibitory effects of essential oil of a compound Chinese herbal medicine Weiqi Decoction (WQD) on AGS cell proliferation were to be investigated. Methods: Chemical compounds of WQD essential oil were detected by gas chromatography and mass spectrometry analysis. Cell viability was measured by methyl thiazolyl tetrazolium method. Cell cycle distribution was detected by flow cytometry. Apoptosis and necrosis of AGS cells were determined by Hoechst 33342/propidium iodine staining. Results: Chemical analysis showed that the main ingredients of WQD essential oil were bornylene and 3-n-butylphthalide. Ligustilide, which is the effective compound of Danggui (Radix Angelicae Sinensis), was not detected in WQD essential oil. The essential oil inhibited cell proliferation in a dose- and time-dependent manner, and blocked cell cycle progression at G(2)/M stage. At the concentrations that resulted in significant inhibition of cell proliferation and cell cycle arrest, essential oil induced both apoptosis and necrosis. Conclusion: The results suggest that WQD essential oil contains some effective ingredients for treating chronic atrophic gastritis and functional dyspepsia, and also has an antiproliferative effect on AGS cells through cell cycle arrest and apoptosis promotion in vitro. Therefore, essential oil should be retained as much as possible during stewing this decoction.

Objective As estrogen increases visceral hypersensitivity induced by water avoidance stress in female rats,further experiment was designed to determine whether the influence of estrogen involves NR2B.Methods Healthy adult female Wistar rats were bilaterally ovariectomized,and then housed individually.Implantation of cannula into lateral cerebroventricle and electrodes into the abdominal muscle had been done.After 5 days recovery,rats with abnormal behavior and ehctromyography were excluded,finally a total of 48 rats were eligible,and were equipped for abdominal muscle electromyography and submitted to water avoidance stress(WAS).Visceromotor response(VMR)to 20,40,60 and 80 mmHg colorectal distension(CRD)was recorded in rats intracerebroventricular-infused with either 17β-estradiol,normal saline,AP5(NMDA receptor-antagonist)or Ro25-6981(NR2B antagonist).NR2B mRNA in anterior cingulate cortex or dorsal root ganglia were compared by real-time PCR between the rats treated with 17β-estradioI and that with normal saline.Results Bilaterally ovarieetomized rats treated with 17β-estradiol,exhibited more visceral hypersensitivity after WAS than that with normal saline on 40,60 and 80 mmHg CRD(P=0.039,P=0.033,P=O.001).The VMR on 40 and 60 mmHg CRD in 17β-estradiol treated group was not significantly different from that in 17β-estradiol plus Ro25-6981 treated group.Whilst,significant differences of VMR were noted between 17β-estradiol treated group and 17β-estradiol plus Ro25-6981 treated group on 80 mmHg CRD,17β-estradiol treated group and 17β-estradiol plus AP5 treated group on 60,80 nmmHg CRD,respectively.17β-estradiol increased NR2BmRNA in anterior cingulate cortex(0.57±0.41 vs 0.21±0.13,P=0.048),but not in dorsal root ganglia(0.35±0.45 vs 0.38±0.31,P=0.465). Stress-induced visceral hypersensitivity in the hormonally-restored visceral hyper-responsiveness of bilaterally ovariectomized rats was antagonized by AP5 or Ro25-6981.Conclusions Estrogen may be mediated through NR2B activation to enhance visceral sensitivity in female stressed rats,that probably related with the increased expression of NR2B mRNA in anterior cingulate cortex.