In response to issues in diagnostics experiment teaching for intelligent medical engineering in medical colleges and universities, such as the disconnection between theory and practice, the teaching content emphasizing "medicine" rather than "engineering", the lack of interaction between teachers and students, and the single teaching evaluation method, this study introduced the outcome-based education concept. According to the graduation requirements supported by the course, the experiment teaching reform ideas were adjusted from three aspects: setting curriculum objectives based on learning outcomes, reforming teaching strategies to achieve learning outcomes, and assessing learning outcomes to promote continuous improvement. The results of the reform show that students' satisfaction with the content, methods, and assessment of experiment teaching all exceeded 86.66%. The reform successfully integrated medicine and engineering, virtual and practical training, and teaching and ideological and political education. The reform also significantly improved the teaching effectiveness and talent cultivation level of experimental courses from the three dimensions of knowledge learning, ability improvement, and value shaping.

Objective:To analyze the risk factors and prognosis of pyogenic liver abscess (PLA) complicated with myocardial injury.Methods:A retrospective selection was made of 108 PLA patients treated in Beijing Friendship Hospital from January 2016 to June 2022 as the research subjects. Patients were divided into the myocardial injury group and non-myocardial injury group according to the occurrence of myocardial injury. Baseline characteristics, laboratory test data, imaging and etiological examination results, echocardiographic indicators, and prognosis were compared between the two groups. A logistic regression model was used to identify the risk factors for PLA complicated with myocardial injury.Results:Among the 108 PLA patients, 24 cases (22.2%) were complicated with myocardial injury. The age of the myocardial injury group was (71±13)years, which was significantly higher than that of the non-myocardial injury group [(59±14)years, P<0.05]. The levels of neutrophil ratio, C-reactive protein (CRP), procalcitonin (PCT), aspartate transaminase (AST), and D-dimer in the myocardial injury group were significantly higher than those in the non-myocardial injury group, while the levels of platelet count (PLT) and albumin (ALB) were significantly lower, with statistically significant differences (all P<0.05). In terms of blood culture, the positive detection rate in the myocardial injury group was as high as 54.2%(13/24), among which the detection rate of Klebsiella pneumoniae was 41.7%(10/24); the positive rate of blood culture in the non-myocardial injury group was only 17.9%(15/84), and the detection rate of Klebsiella pneumoniae was 14.3%(12/84), with statistically significant differences (all P<0.05). The left ventricular ejection fraction in the myocardial injury group was lower than that in the non-myocardial injury group, and the brain natriuretic peptide (BNP) level, the incidence of left ventricular diastolic dysfunction, the proportion of transfer to emergency intensive care unit (EICU), shock, and death were significantly higher than those in the non-myocardial injury group (all P<0.05). Logistic regression analysis showed that age was an independent risk factor for myocardial injury in PLA patients ( P<0.05), and for each 1-year increase in age, the risk of myocardial injury increased by 11.9%. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic value of age for myocardial injury, with an area under the curve (AUC) of 0.729( P<0.05), and the cut-off value calculated by Youden index was 64.5 years. Conclusions:Age is an independent risk factor for myocardial injury in PLA patients. PLA patients complicated with myocardial injury have poor cardiac function, higher incidence of transfer to EICU, shock, and death, and poor prognosis.

OBJECTIVE By conducting evidence evaluation research on literatures,this study aims to reveal the o-verall characteristics and research hotspots in the field of combat trauma-related infections,ultimately providing data support for the prevention and control of such infections.METHODS Relevant research in this field was sys-tematically collected from open-source databases to construct a dataset.The overall characteristics,research hotspots,prevention and control strategies,and future challenges of trauma-related infections were summarized and analyzed.RESULTS From 2004 to 2024,184 papers were published.The United States contributed the most publications,with Uniformed Services University of the Health Sciences being the most productive research insti-tution and Professor Clinton K.Murray as the author with the highest number of publications.The top five key-words with the highest frequency were combat related injury,infection,Acinetobacter baumannii,epidemiology and management.Among the publicly published literature data on war trauma-related infections,blast injuries ac-counted for the largest proportion,mainly multi-site injuries,with limb injuries being the most common.Bacteri-al infections were more common than fungal infections,with gram-negative bacteria being predominant and A.baumannii being the most common.Besides early wound management and the use of antibacterial drugs,in-creased attention should be paid to infection prevention and control in austere environments and the development of novel countermeasures.These advancements are critical to address projected changes in combat trauma,inclu-ding increasingly complex injuries and substantially elevated risks of infection and antimicrobial resistance.CONCLUSION This study systematically presents research hotspots,developmental trends,and prospects in com-bat trauma-related infections through evidence evaluation study,providing novel perspectives for researchers and facilitating further development in this field.

Background Atherosclerotic cardiovascular disease (ASCVD) is a major contributor to the global burden of cardiovascular diseases. However, evidence from meta-analyses on the association between ambient ozone exposure and ASCVD risk remains relatively insufficient. Objective To explore the epidemiological association between ambient ozone exposure and ASCVD, providing scientific evidence for ASCVD prevention and control from the perspective of environmental risk factor management. Methods We systematically searched PubMed, Web of Science, Embase, the Cochrane Library, CNKI, Wanfang Database, CBM, and VIP for published epidemiological studies on the relationship between ambient ozone exposure and ASCVD from January 2007 to December 2023. We performed quality assessment and data extraction of the included studies, and utilized meta-analysis to evaluate the effects of short-term and long-term ozone exposure on different ASCVD outcomes, including mortality and incidence of ischemic heart disease (IHD) and ischemic stroke (IS). Results A total of 24 studies were included based on a set of predetermined eligibility criteria. The meta-analysis results indicated that short-term ozone exposure was associated with an increased risk of ASCVD mortality and incidence. Specifically, short-term ozone exposure was significantly associated with an elevated risk of IHD mortality (combined RR=1.011, 95%CI: 1.008, 1.015; P < 0.05). Additionally, short-term ozone exposure was significantly linked to increased IS mortality (combined RR=1.005, 95%CI: 1.003, 1.008; P < 0.05) and incidence (combined RR=1.015, 95%CI: 1.003, 1.027; P < 0.05). Conclusion Short-term exposure to ambient ozone significantly elevates acute cardiovascular disease risk. However, the epidemiological association between long-term ozone exposure and ASCVD remains inconclusive. Future high-quality cohort studies with refined exposure assessment methods are warranted to elucidate the chronic cardiovascular effects of ozone exposure.

Volatile organic compounds (VOCs) and semi-volatile organic compounds (SVOCs) are common organic compounds in indoor and outdoor air, and enter the human body primarily through the respiratory tract and directly damage the respiratory system. Previous studies have suggested that exposure to VOCs/SVOCs may associate with the prevalence, incidence, and progression of asthma, but the extent of the associations is still vague. Furthermore, biomarkers for efficient and simple asthma diagnosis, typing, and attack prediction remain unclear at this stage. From the perspective of the collection and detection methods of VOCs/SVOCs, this paper summarized the epidemiological associations and underlying biological mechanisms between VOCs/SVOCs exposure and the prevalence, incidence, and progression of asthma in children/adults. It also demonstrated the application of VOCs/SVOCs in recent years in assisting asthma diagnosis, such as distinguishing asthma patients from the healthy population, differentiating different asthma phenotypes, and predicting asthma acute exacerbations, aiming to provide a scientific basis for improving current asthma management.

In response to issues in diagnostics experiment teaching for intelligent medical engineering in medical colleges and universities, such as the disconnection between theory and practice, the teaching content emphasizing "medicine" rather than "engineering", the lack of interaction between teachers and students, and the single teaching evaluation method, this study introduced the outcome-based education concept. According to the graduation requirements supported by the course, the experiment teaching reform ideas were adjusted from three aspects: setting curriculum objectives based on learning outcomes, reforming teaching strategies to achieve learning outcomes, and assessing learning outcomes to promote continuous improvement. The results of the reform show that students' satisfaction with the content, methods, and assessment of experiment teaching all exceeded 86.66%. The reform successfully integrated medicine and engineering, virtual and practical training, and teaching and ideological and political education. The reform also significantly improved the teaching effectiveness and talent cultivation level of experimental courses from the three dimensions of knowledge learning, ability improvement, and value shaping.

OBJECTIVE By conducting evidence evaluation research on literatures,this study aims to reveal the o-verall characteristics and research hotspots in the field of combat trauma-related infections,ultimately providing data support for the prevention and control of such infections.METHODS Relevant research in this field was sys-tematically collected from open-source databases to construct a dataset.The overall characteristics,research hotspots,prevention and control strategies,and future challenges of trauma-related infections were summarized and analyzed.RESULTS From 2004 to 2024,184 papers were published.The United States contributed the most publications,with Uniformed Services University of the Health Sciences being the most productive research insti-tution and Professor Clinton K.Murray as the author with the highest number of publications.The top five key-words with the highest frequency were combat related injury,infection,Acinetobacter baumannii,epidemiology and management.Among the publicly published literature data on war trauma-related infections,blast injuries ac-counted for the largest proportion,mainly multi-site injuries,with limb injuries being the most common.Bacteri-al infections were more common than fungal infections,with gram-negative bacteria being predominant and A.baumannii being the most common.Besides early wound management and the use of antibacterial drugs,in-creased attention should be paid to infection prevention and control in austere environments and the development of novel countermeasures.These advancements are critical to address projected changes in combat trauma,inclu-ding increasingly complex injuries and substantially elevated risks of infection and antimicrobial resistance.CONCLUSION This study systematically presents research hotspots,developmental trends,and prospects in com-bat trauma-related infections through evidence evaluation study,providing novel perspectives for researchers and facilitating further development in this field.

Objective:To analyze the risk factors and prognosis of pyogenic liver abscess (PLA) complicated with myocardial injury.Methods:A retrospective selection was made of 108 PLA patients treated in Beijing Friendship Hospital from January 2016 to June 2022 as the research subjects. Patients were divided into the myocardial injury group and non-myocardial injury group according to the occurrence of myocardial injury. Baseline characteristics, laboratory test data, imaging and etiological examination results, echocardiographic indicators, and prognosis were compared between the two groups. A logistic regression model was used to identify the risk factors for PLA complicated with myocardial injury.Results:Among the 108 PLA patients, 24 cases (22.2%) were complicated with myocardial injury. The age of the myocardial injury group was (71±13)years, which was significantly higher than that of the non-myocardial injury group [(59±14)years, P<0.05]. The levels of neutrophil ratio, C-reactive protein (CRP), procalcitonin (PCT), aspartate transaminase (AST), and D-dimer in the myocardial injury group were significantly higher than those in the non-myocardial injury group, while the levels of platelet count (PLT) and albumin (ALB) were significantly lower, with statistically significant differences (all P<0.05). In terms of blood culture, the positive detection rate in the myocardial injury group was as high as 54.2%(13/24), among which the detection rate of Klebsiella pneumoniae was 41.7%(10/24); the positive rate of blood culture in the non-myocardial injury group was only 17.9%(15/84), and the detection rate of Klebsiella pneumoniae was 14.3%(12/84), with statistically significant differences (all P<0.05). The left ventricular ejection fraction in the myocardial injury group was lower than that in the non-myocardial injury group, and the brain natriuretic peptide (BNP) level, the incidence of left ventricular diastolic dysfunction, the proportion of transfer to emergency intensive care unit (EICU), shock, and death were significantly higher than those in the non-myocardial injury group (all P<0.05). Logistic regression analysis showed that age was an independent risk factor for myocardial injury in PLA patients ( P<0.05), and for each 1-year increase in age, the risk of myocardial injury increased by 11.9%. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic value of age for myocardial injury, with an area under the curve (AUC) of 0.729( P<0.05), and the cut-off value calculated by Youden index was 64.5 years. Conclusions:Age is an independent risk factor for myocardial injury in PLA patients. PLA patients complicated with myocardial injury have poor cardiac function, higher incidence of transfer to EICU, shock, and death, and poor prognosis.

Objective To study the effects and mechanism of norcantharidin(NCTD)on proliferation and apoptosis of NB4 and K562 human leukemic cells by regulating phosphoprotein phosphatase 5 catalytic(PPP5C).Methods PC3.1 and PPP5C-PC3.1 plasmids were electroporated into NB4 and K562 cells.Stable NB4 and K562 cell lines were selected with geneticin(G418).Protein and mRNA expression levels of PPP5C were measured by Western blot and RT-qPCR,respectively.Proliferation,migration,and apoptosis of NB4 and K562 cells were determined by a CCK-8 assay,transwell assay,and Live & Dead? animal cell viability/toxicity detection kit,respectively.NB4 and K562 cells were divided into 0 μg/mL NCTD group and various NCTD dose groups,and cultured in RPMI 1640 medium containing 0,8,16,or 32 μg/ml NCTD.The Live & Dead? animal cell viability/toxicity detection kit measured the numbers of dead and live cells,and cell morphology was observed under a microscope.Western blot was used to measure protein expression levels of caspase 3,Cleaved caspase 3,JNK,p-JNK,p38,p-p38,and α-Tubulin.Results Proliferation,migration,and apoptosis of NB4 and K562 cells were enhanced by overexpression of PPP5C.Compared with 0 μg/mL NCTD group,NCTD promoted apoptosis in a dose-dependent manner.PPP5C overexpression antagonized the killing effect of NCTD on leukemic cells.Mechanistic investigations showed that PPP5C reduced the protein level of p-JNK by dephosphorylating and regulating the expression of apoptosis-related protein Cleaved caspase 3.Conclusions NCTD promotes apoptosis of NB4 and K562 cells and inhibits their proliferation by inhibiting PPP5C.

Objective To explore the impact of pH value of the reaction system on the properties of bovine hemoglobin modified with aldehydeated polyethylene glycol(PEG-bHb).Methods PEG-bHb conjugates were synthesized under varying pH conditions(6.0,6.5,7.4 and 8.0)of the reaction system while consistent molar ratios,temperature,and reaction time were maintained.The structural and functional attributes of PEG-bHb were characterized.Results The proportion of methemoglobin decreased with an increase in pH.In a weakly acidic reaction environment,the PEG-bHb was found to be relatively highly modified.At pH 6.5,the average number of PEG chains attached to the bHb surface was 6.86±0.38.Selective PEG modification of the N-terminal α-NH2 groups was more pronounced under weakly acidic conditions.Specifically,at pH 6.5,the modification efficiency of the N-terminal α-NH2 groups of bHb by aldehyde-activated PEG reached 95.4％for the α-chains and 99.3％for the β-chains.The PEG modification influenced the heme region microenvi-ronment of bHb,with minimal structural impact observed at pH 6.5.After modification,the oxygen affinity of PEG-bHb was enhanced,the Hill coefficient was reduced,and there were significant increases in colloid osmotic pressure,viscosity,and particle size,all of which differed markedly from the unmodified bHb group(P＜0.001).Conclusion The synthesis of PEG-bHb under weakly acidic conditions can result in a high degree of selective modification of the N-terminal α-NH2 groups and an overall high degree of modification.