Objective To explore the potential neural mechanism of adolescent schizophrenia by analysis of the resting state functional connectivity(rs-FC) network of posterior cingulate gyrus(PCC) with functional magnetic resonance imaging (fMRI).Methods Twenty-one first-episode medication-naive adolescent patients with schizophrenia were recruited from outpatients and inpatients in the Sichuan Mental Health Center from December 2016 to January 2018,and 21 gender-,age-and education-matched healthy ones were as control.The illness severity was estimated by PANSS and Psychotic Symptom Rating Scales (PSYRAT).All subjects was scanned by resting-state brain fMRI.The PCC was defined as a seed region of interest(ROI) after the imaging preprocessing,and the functional connectivity between seed region and other brain regions were calculated after the imaging data preprocessing.Statistical analysis of image data was processed by REST software.Results Data of 19 patients and 21 healthy volunteers were included in statistical analysis after realignment.Compared to the control group,the patients group exhibited increased PCC rs-FC (P＜0.05,AlphaSim-corrected for multiple comparisons,cluster size＞40) in two brain regions including the left orbital part of inferior frontal gyrus extended to the left superior temporal gyrus (MNI:x=-45,y=27,z=-15;t=5.164) and the bilateral middle cingulate gyrus (MNI:x=-6,y=-15,z=30;t=4.789),and the weaker PCC rs-FC (P＜0.05,AlphaSim-corrected for multiple comparisons,cluster size＞40) with the left postcentral gyrus(MNI:x=60,y=-12,z=45;t=-3.585) and the fight postcentral gyrus (MNI:x=-24,y=-33,z=66;t=-4.932).The rs-FC between the left orbital part of inferior frontal gyrus and the PCC was positively correlated with the PANSS negative symptom scores (r=0.487,P=-0.034).Conclusion It showed altered rs-FC of the PCC related network in first-episode adolescent schizophrenia patients.The increased rs-FC between the PCC and the orbitofrontal cortex may be associated with negative symptoms in adolescent schizophrenia patients.

Objective To explore the potential neural mechanism of adolescent schizophrenia by analysis of the resting state functional connectivity(rs-FC) network of posterior cingulate gyrus(PCC) with functional magnetic resonance imaging (fMRI).Methods Twenty-one first-episode medication-naive adolescent patients with schizophrenia were recruited from outpatients and inpatients in the Sichuan Mental Health Center from December 2016 to January 2018,and 21 gender-,age-and education-matched healthy ones were as control.The illness severity was estimated by PANSS and Psychotic Symptom Rating Scales (PSYRAT).All subjects was scanned by resting-state brain fMRI.The PCC was defined as a seed region of interest(ROI) after the imaging preprocessing,and the functional connectivity between seed region and other brain regions were calculated after the imaging data preprocessing.Statistical analysis of image data was processed by REST software.Results Data of 19 patients and 21 healthy volunteers were included in statistical analysis after realignment.Compared to the control group,the patients group exhibited increased PCC rs-FC (P＜0.05,AlphaSim-corrected for multiple comparisons,cluster size＞40) in two brain regions including the left orbital part of inferior frontal gyrus extended to the left superior temporal gyrus (MNI:x=-45,y=27,z=-15;t=5.164) and the bilateral middle cingulate gyrus (MNI:x=-6,y=-15,z=30;t=4.789),and the weaker PCC rs-FC (P＜0.05,AlphaSim-corrected for multiple comparisons,cluster size＞40) with the left postcentral gyrus(MNI:x=60,y=-12,z=45;t=-3.585) and the fight postcentral gyrus (MNI:x=-24,y=-33,z=66;t=-4.932).The rs-FC between the left orbital part of inferior frontal gyrus and the PCC was positively correlated with the PANSS negative symptom scores (r=0.487,P=-0.034).Conclusion It showed altered rs-FC of the PCC related network in first-episode adolescent schizophrenia patients.The increased rs-FC between the PCC and the orbitofrontal cortex may be associated with negative symptoms in adolescent schizophrenia patients.

Objective To explore the inhibitory effect of 24 pseudomonas aeruginosa(PA) on pathogenic fungi ,such as candida albicans ,candida tropicalis ,candida glabrata ,candida parapsilosis ,candida krusei ,mucous spore bacterium (MSB) etc .Methods 24 PA isolates were collected from clinical specimens and identified by Gram′s stain ,oxidase production and the API 20NE system(bi-oMerieux ,France) .Cross-streaking method and sterilizing filter paper-disk method and co-cultured method were applied to observe the inhibitory effect of PA .Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analyzed the difference of bacte-rial proteins of PA .Results The results showed that some strains of 24 PA had strong inhibitory effect against pathogenic fungi , some strain had partial effect and others had no effect .Co-cultured test showed that PA could inhibit the growth of fungal hyphae . SDS-PAGE displayed the significant difference in secretive proteins between the PA strains which had strong effect and no effect . Conclusion PA have inhibitory effect upon common pathogenic fungi and and this might be related to inhibit fungal hyphae forma-tion ,various protein secretion and inhibit the growth of fungi .

Objective To study the expressions of Toll-like receptor 9 protein (TLR9) in peripheral B and T lymphocytes in newly diagnosed, untreated patients with systemic lupus erythematosus (SLE) and their relationship with clinical parameters. Methods Blood samples were obtained from 35 newly diag-nosed, untreated patients with SLE and 16 healthy human controls. B, T lymphocytes and TLR9 protein were labeled with fluorescent antibodies, and the expressions of TLR9 protein were detected by flow cytometry in peripheral B and T lymphocytes. The relationship between TLR9 expression and clinical parameters was assessed. Results The proportions of B and T lymphocytes expressing TLR9 in newly diagnosed, untreated patients were (53.94±17.95)% and (49.33 ± 23.30)%, respectively, compared to (29.40 ± 10.54)% and (29.18 ± 14.78)%, respectively, in healthy controls (t = 6.11,3.73, respectively, both P < 0.01). Additionally,the proportion of B lymphocytes expressing TLR9 correlated negatively with SLE disease activity index (SLEDAI)(r = -0.39, P < 0.05), but positively with the level of serum IgA antibody (r = 0.74, P < 0.01).Condnsions The expression of TLR9 is elevated in peripheral T and B lymphocytes from patients with newly diagnosed, untreated SLE, and the proportion of TLR9-expressing B lymphocytes negatively correlates with SLEDAI, but positively correlates with the serum level of IgA antibody.