Objective:To evaluate the music perception ability and appraisal status of cochlear implant adult users, then to compare those with normal-hearing subjects.Methods:The Iowa Musical Background and Appreciation Questionnaire (IMBAQ) was conducted to evaluate 20 cases of post-lingual deafness cochlear implant recipients (9 males, 11 females) aged 12-61(mean=28.8 yrs). Those music perception abilities were tested via Music Perceptional Evaluation Platform. Two kinds of tone(C#3, C#5)were carried out in the music perception test, including the Just Noticeable Difference (JND) Test, the Pitch-Direction Discrimination (PDD) Test and the Melody Contour Identification (MCI) Test. Meanwhile, the timbre identification tests were received by both normal hearing and cochlear implant patients.Results:The total outcomes of cochlear implant recipients were inferior to the normal hearing subjects ( P<0.05). Both of the thresholds of JND ( P=0.001) and PDD ( P<0.001) had a significant difference, while comparing the two frequency values (C#3 and C#5) in cochlear implant adult users. IMBAQ results indicated the lower post-implantation music appreciation and engagement compared to pre-deafness levels. The longer duration of formal music training was positively correlated with better music perception performance in CI recipients. Conclusions:There still has a big gap between the cochlear implant group and normal hearing group in the music perception test. And different frequency value causes effect on the result of cochlear implant patient group.

Objective: To determine the expression of melanoma-associated antigens D4(MAGED4) and SIRT7 in human glioma, and to analyze the potential effects of MAGED4 and SIRT7 on glioma cell proliferation. Methods: The MAGED4 and SIRT7 expression levels and their correlation were compared by the China glioma genome atlas(CGGA), human protein atlas(HPA), and UALCAN databases. Survival analysis, ROC curve analysis, and Cox regression analysis were used to predict the outcome of MAGED4 and SIRT 7 in glioma patients. Gene ontology(GO) and Kyoto encyclopedia of genes and genomes(KEGG) signaling pathway enrichment analysis were used to explore the biological functions of MAGED4 and SIRT7 in glioma. Western blot experiment was used to investigate whether MAGED4 protein exerted its regulatory effects on the activity of the PI3K/AKT signaling pathway via SIRT7. The effect of MAGED4 on cell proliferation in glioma through SIRT7 was explored by CCK-8. Results: The analysis results of CGGA, UALCAN, and HPA databases showed that the expression levels of MAGED4 and SIRT7 in glioma tissues were higher than those in normal brain tissue, and the expression were positively correlated. Results of survival, ROC, and Cox analysis showed that high expression of MAGED4 and SIRT7 mRNA were risk factors for poor prognosis in glioma. Results of KEGG enrichment analysis showed that MAGED4 and SIRT7 were associated with the PI3K/AKT signaling in glioma, and Western blot results showed that MAGED4 activated the PI3K/AKT signaling pathway by regulating SIRT7. The CCK-8 results showed that MAGED4 promotes the proliferation of glioma cells through SIRT7. Conclusion MAGED4 and SIRT7 are highly expressed in glioma and associated with poor prognosis, and MAGED4 promotes glioma cell proliferation through activation of the PI3K/AKT signaling pathway by SIRT7.

Objective:To evaluate the music perception ability and appraisal status of cochlear implant adult users, then to compare those with normal-hearing subjects.Methods:The Iowa Musical Background and Appreciation Questionnaire (IMBAQ) was conducted to evaluate 20 cases of post-lingual deafness cochlear implant recipients (9 males, 11 females) aged 12-61(mean=28.8 yrs). Those music perception abilities were tested via Music Perceptional Evaluation Platform. Two kinds of tone(C#3, C#5)were carried out in the music perception test, including the Just Noticeable Difference (JND) Test, the Pitch-Direction Discrimination (PDD) Test and the Melody Contour Identification (MCI) Test. Meanwhile, the timbre identification tests were received by both normal hearing and cochlear implant patients.Results:The total outcomes of cochlear implant recipients were inferior to the normal hearing subjects ( P<0.05). Both of the thresholds of JND ( P=0.001) and PDD ( P<0.001) had a significant difference, while comparing the two frequency values (C#3 and C#5) in cochlear implant adult users. IMBAQ results indicated the lower post-implantation music appreciation and engagement compared to pre-deafness levels. The longer duration of formal music training was positively correlated with better music perception performance in CI recipients. Conclusions:There still has a big gap between the cochlear implant group and normal hearing group in the music perception test. And different frequency value causes effect on the result of cochlear implant patient group.

Objective To observe the clinical efficacy of Dulongneedling in the treatment of vertigo in cervical spondylosis of vertebral artery(CSA).Methods A total of 46 cases of patients with definitive diagnosis of vertigo in CSA admitted to the outpatient clinic of the Acupuncture,Muxibustion and Rehabilitation Department of Guangdong Second Traditional Chinese Medicine Hospital from June to October 2024 were selected for the study.The patients were randomly divided into observation group and control group according to the random number table method,with 23 cases in each group.The observation group was treated with Dulongten-needling in shoulder and neck,and the control group was given conventional acupuncture treatment,the course of treatment for the two groups covered two weeks.After two weeks of treatment,the clinical efficacy of the two groups was evaluated,and the changes in the scores of Evaluation Scale For Cervical Vertigo(ESCV)before and after treatment were observed in the patients of the two groups.Three dimensional pseudo-continuous arterial spin labeling(3D-pCASL)technique was applied to determin the changes in cerebral blood flow(CBF)values in the region of region of interest(ROI),and the changes in CBF values in the patients of the two groups were compared.Results(1)The total effective rate of the observation group was 91.30%(21/23),and that of the control group was 78.26%(18/23),and the efficacy of the observation group was superior to that of the control group,the difference being statistically significant(P<0.05).(2)After treatment,the ESCV scores of patients in the two groups were significantly improved(P<0.05),and the improvement in the observation group was significantly superior to that in the control group,the difference being statistically significant(P<0.05).(3)After treatment,the CBF change values of each ROI in the two groups showed elevated(positive),and the CBF change values of the right middle cerebral artery(R-MCA)in the control group showed decreased(negative).After treatment,the CBF change values of the observation group in the blood supply areas of the right posterior inferior cerebellar artery(R-PICA),right anterior inferior cerebellar artery(R-AICA),right anterior cerebral artery(R-ACA),branches from vertebral arteries(VA)and basilar arteries(BA),bilateral superior cerebellar arteries(SCA),and bilateral posterior cerebral arteries(PCA)were significantly elevated,and the observation group was significantly superior in improving the above ROIs in the CBF change value to that of the control group,the difference being statistically significant(P<0.05).Conclusion Dulongneedling in the treatment of vertigo in CSA can significantly improve the CBF of patients,reduce the clinical symptoms such as vertigo,neck and shoulder pain,headache.

Objective To explore the risk factors analysis and prediction model establishment of restenosis dysfunction at 1 year after percutaneous transluminal angioplasty(PTA)of internal arteriovenous fistula based on machine learning.Methods A total of 322 patients who underwent PTA of internal arteriovenous fistula in Wenzhou Central Hospital from June 1,2018 to December 31,2023 were enrolled.The operation-related data were collected.Variables were used to construct prediction models using five machine learning algorithms:Random forest(RF),extreme gradient boosting(XGBoost),support vector machine(SVM),gradient boosting decision tree(GBDT)and Logistic regression(LR).The predictive efficacy was evaluated by area under receiver operating characteristic curve.Results There were 97 cases of restenosis dysfunction and 225 cases of non-dysfunction.The incidence of internal fistula restenosis dysfuction was 30.1％1 year after PTA.The age,diabetes,smoking,calcium-phosphorus product,dilatation pressure ≥20mmHg,and balloon diameter ≥6mnm in dysfunction group were higher than those in non-dysfunction group.The difference was statistically significant(P＜0.05).The area under the curve of RF,XGBoost,SVM,GBDT and LR models based on machine learning was 0.908(95％CI:0.836-0.980),0.809(95％CI:0.696-0.922),0.745(95％CI:0.624-0.867),0.711(95％CI:0.576-0.847)and 0.651(95％CI:0.508-0.795),respectively.The sensitivity was 79.1％,70.8％,83.3％,62.5％and 72.3％,respectively.The specificity was 89.0％,81.2％,57.8％,78.9％and 71.0％,respectively.Conclusion Age,diabetes mellitus,smoking,calcium-phosphorus product,expansion pressure ≥20mnmHg,balloon diameter ≥ 6mm are independent risk factors for restenosis failure after PTA in patients with internal arteriovenous fistula,which can be used as an index to predict restenosis failure 1 year after PTA in internal arteriovenous fistula.The random forest prediction model based on machine learning algorithm has good prediction performance and can better predict restenosis failure 1 year after PTA in internal arteriovenous fistula.

Objective:To construct nomogram model based on lymph node imaging parameters for predicting the risk of lymph node metastasis in lung malignancies.Methods:From November 2020 to September 2022, 34 patients (23 males, 11 females, age (65.8±6.8) years) diagnosed with lung malignancies by pathology at the Affiliated Hospital of Inner Mongolia Medical University were prospectively collected. Based on enhanced CT and 99Tc m-hydrazinonicotinamide-(polyethylene glycol) 4-E((polyethylene glycol) 4-c((Arg-Gly-Asp)fK)) 2 (HYNIC-PEG 4-E(PEG 4-c(RGDfK)) 2; 3PRGD 2)SPECT/CT imaging, referring to the mediastinal lymph node zoning standards formulated by the International Association for the Study of Lung Cancer, lymph nodes with clear pathological properties and imaging locations were included in the study. Lymph nodes were randomly divide into a training group and a validation group at a ratio of 7∶3. Differences of imaging parameters between positive and negative lymph node metastasis were compared by independent-sample t test or Mann-Whitney U test or χ2 test. Parameters with statistical differences were incorporate into the multivariate logistic regression equation, and a joint variable diagnostic model for predicting lymph node metastasis was generated. The potential of the model was evaluate by ROC curve analysis, calibration, and decision curve analysis (DCA). Results:Among 34 patients with malignant lung tumors, 11 had lymph node metastasis. A total of 174 lymph nodes met the inclusion criteria were randomly divided into a training group of 114 nodes and a validation group of 60 nodes. In the training group and validation group, there were statistically significant differences in lymph node length, lymph node short diameter, lymph node length/short diameter, necrosis, lymph node to mediastinal blood pool radioactive count ratio (T/B), lymph node to liver radioactive count ratio (T/L), lymph node to muscle radioactive count ratio (T/M), and lymph node enhancement mode between patients with positive and negative lymph node metastasis ( χ2 values: 3.89-34.06, t values: 2.31-3.87, Z values: from -3.63 to -2.30, all P<0.05). The lymph node edge was different only in the training group ( χ2=5.62, P=0.018). Finally, the lymph node length/short diameter, edge, necrosis, T/B, T/L, T/M, and lymph node enhancement modes were included in the multivariate logistic regression prediction model, with the AUCs of 0.878 and 0.949 in the training and validation groups, respectively. The calibration curve showed good consistency between the predicted results and the actual results, and DCA showed that the nomogram had clinical practicality. Conclusion:The nomogram model constructed based on the imaging parameters of lymph nodes can evaluate the risk of lymph node metastasis in patients with lung malignancies, providing a convenient and objective tool for determining staging and developing treatment plans.

Objective To explore the risk factors analysis and prediction model establishment of restenosis dysfunction at 1 year after percutaneous transluminal angioplasty(PTA)of internal arteriovenous fistula based on machine learning.Methods A total of 322 patients who underwent PTA of internal arteriovenous fistula in Wenzhou Central Hospital from June 1,2018 to December 31,2023 were enrolled.The operation-related data were collected.Variables were used to construct prediction models using five machine learning algorithms:Random forest(RF),extreme gradient boosting(XGBoost),support vector machine(SVM),gradient boosting decision tree(GBDT)and Logistic regression(LR).The predictive efficacy was evaluated by area under receiver operating characteristic curve.Results There were 97 cases of restenosis dysfunction and 225 cases of non-dysfunction.The incidence of internal fistula restenosis dysfuction was 30.1％1 year after PTA.The age,diabetes,smoking,calcium-phosphorus product,dilatation pressure ≥20mmHg,and balloon diameter ≥6mnm in dysfunction group were higher than those in non-dysfunction group.The difference was statistically significant(P＜0.05).The area under the curve of RF,XGBoost,SVM,GBDT and LR models based on machine learning was 0.908(95％CI:0.836-0.980),0.809(95％CI:0.696-0.922),0.745(95％CI:0.624-0.867),0.711(95％CI:0.576-0.847)and 0.651(95％CI:0.508-0.795),respectively.The sensitivity was 79.1％,70.8％,83.3％,62.5％and 72.3％,respectively.The specificity was 89.0％,81.2％,57.8％,78.9％and 71.0％,respectively.Conclusion Age,diabetes mellitus,smoking,calcium-phosphorus product,expansion pressure ≥20mnmHg,balloon diameter ≥ 6mm are independent risk factors for restenosis failure after PTA in patients with internal arteriovenous fistula,which can be used as an index to predict restenosis failure 1 year after PTA in internal arteriovenous fistula.The random forest prediction model based on machine learning algorithm has good prediction performance and can better predict restenosis failure 1 year after PTA in internal arteriovenous fistula.

Objective:To construct nomogram model based on lymph node imaging parameters for predicting the risk of lymph node metastasis in lung malignancies.Methods:From November 2020 to September 2022, 34 patients (23 males, 11 females, age (65.8±6.8) years) diagnosed with lung malignancies by pathology at the Affiliated Hospital of Inner Mongolia Medical University were prospectively collected. Based on enhanced CT and 99Tc m-hydrazinonicotinamide-(polyethylene glycol) 4-E((polyethylene glycol) 4-c((Arg-Gly-Asp)fK)) 2 (HYNIC-PEG 4-E(PEG 4-c(RGDfK)) 2; 3PRGD 2)SPECT/CT imaging, referring to the mediastinal lymph node zoning standards formulated by the International Association for the Study of Lung Cancer, lymph nodes with clear pathological properties and imaging locations were included in the study. Lymph nodes were randomly divide into a training group and a validation group at a ratio of 7∶3. Differences of imaging parameters between positive and negative lymph node metastasis were compared by independent-sample t test or Mann-Whitney U test or χ2 test. Parameters with statistical differences were incorporate into the multivariate logistic regression equation, and a joint variable diagnostic model for predicting lymph node metastasis was generated. The potential of the model was evaluate by ROC curve analysis, calibration, and decision curve analysis (DCA). Results:Among 34 patients with malignant lung tumors, 11 had lymph node metastasis. A total of 174 lymph nodes met the inclusion criteria were randomly divided into a training group of 114 nodes and a validation group of 60 nodes. In the training group and validation group, there were statistically significant differences in lymph node length, lymph node short diameter, lymph node length/short diameter, necrosis, lymph node to mediastinal blood pool radioactive count ratio (T/B), lymph node to liver radioactive count ratio (T/L), lymph node to muscle radioactive count ratio (T/M), and lymph node enhancement mode between patients with positive and negative lymph node metastasis ( χ2 values: 3.89-34.06, t values: 2.31-3.87, Z values: from -3.63 to -2.30, all P<0.05). The lymph node edge was different only in the training group ( χ2=5.62, P=0.018). Finally, the lymph node length/short diameter, edge, necrosis, T/B, T/L, T/M, and lymph node enhancement modes were included in the multivariate logistic regression prediction model, with the AUCs of 0.878 and 0.949 in the training and validation groups, respectively. The calibration curve showed good consistency between the predicted results and the actual results, and DCA showed that the nomogram had clinical practicality. Conclusion:The nomogram model constructed based on the imaging parameters of lymph nodes can evaluate the risk of lymph node metastasis in patients with lung malignancies, providing a convenient and objective tool for determining staging and developing treatment plans.

Objective To investigate the effects of DNA demethylation drugs combined with histone deacetylase in-hibitors on fragile X mental retardation 1 neighbor protein (FMR1NB) expression and its promoter methylation in human oral cancer cells and try to find a strategy of weakening the heterogeneity of FMR1NB expression.Methods Human oral cancer cell lines Cal27 and SCC-9 were treated with decitabine (DAC) , an inhibitor of DNA meth-yltransferase, combined with trichostatin A (TSA) and valproic acid (VPA), inhibitors of histone deacetylase.Then reverse transcription-polymerase chain reaction (RT-PCR) , quantitative real-time PCR (qRT-PCR) and Western blot were used to detect the expression of FMR1 NB and pyrosequencing was used to detect the methylation of FMR1NB promoter.Results Compared with the blank control group, DAC and its combination with TSA and VPA significantly induced the expression of FMR1NB mRNA and protein in Cal27 and SCC-9 cells.Compared with DAC alone group, FMR1NB mRNA expression of each DAC-combined drug groups significantly increased, but FMR1NB protein did not significantly change in Cal27 cells; for SCC-9 cells, except for DAC+TSA group, the mRNA and protein levels of FMR1NB significantly increased in all other groups.In addition, there was no signifi-cant difference in the expression of FMR1 NB mRNA and protein between the three-combined drugs group and two-combined drugs groups.Further methylation assay showed that the methylation level of the overall FMR1NB promot-er and its each CpG site measured were reduced to varying degrees in all treatment groups except for three-combina-tion drug group of SCC-9.Conclusion DAC and its combination with TSA and VPA can enhance the expression of FMR1NB by mediating the demethylation of FMR1NB promoter, wherein the enhanced expression effect of the com-bination of the two drugs is stronger, suggesting that they have the potential to weaken the heterogeneity of FMR1NB expression and improve the immunotherapy effect of oral cancer.

Objective To detect the expression profile of heme oxygenase-1(HO-1)during the process of wound repair in radiation-wound combined injury(R-W-CI),and evaluate its wound healing improving effects of R-W-CI by HO-1 knockdown with siRNA.Methods A total of 36 male C57BL/6J mice(8 weeks old)were randomly and equally divided into a simple skin wound group(W group)and a skin wound group combined with whole-body radiation(6 Gy)injury(R-W-CI group).During the wound healing process,the wounds were photographed and recorded,and the residual areas were quantified by Image J.Wound tissues were sampled and stained with HE staining for pathological and histological observation,and the damage to the hematopoietic system was assessed by dynamic examination of the peripheral blood.The expression and changes of HO-1 in wound tissues were detected by q-PCR and Western blotting.Then,26 male C57BL/6J mice(8 weeks old)were randomly and equally divided into siRNA knockdown HO-1 group(si-HO-1 group)and siRNA negative control group(si-NC group).After radiation combined injury was inflicted,60 μL of F127 gel loaded with si-HO-1(5 μm/L)was applied to each wound in the si-HO-1 group,and an equal amount of F127 gel loaded with negative control si-NC was applied to the wound in the si-NC group.The knockdown of HO-1 in wound tissues was detected by Western blotting,and the changes in wound area were observed.In the wound tissues harvested in 3 d after wounding,the expression of cytokines IL-1β,IL-6 and TNF-α was examined by q-PCR and the proliferation of granulation tissues was evaluated by Ki67 immunohistochemical staining.HE staining was performed on wound tissues on day 3 and day 9 post-injury to assess the improvement effect of knockdown of HO-1 on wound healing of radiation combined injuries.Results Compared with the W group,semi-quantitative analysis of the residual wound area showed that healing was significantly delayed in the R-W-CI group on days 7 and 10 post-injury(P＜0.01).HE staining on day 7 showed that in the R-W-CI group,the re-epithelialization was delayed,and the growth of granulation tissues was poor;and at the same time,peripheral blood leukocytes and their classified counts showed a significant decrease in the early period after injury(P＜0.05).Further tests indicated that the expression of HO-1 protein was slightly higher in the wound of the R-W-CI group than that of the W group in 3 and 7 d after injury,though no significant difference(P＞0.05),whereas statistical difference was seen in 10 d(P＜0.05),accompanied by the distribution of the full-length and truncated forms of HO-1 protein.Quantitative PCR obtained similar results in the mRNA expression of HO-1 in wounds in both 7 and 10 d after injury(P＜0.05).siRNA intervention could effectively knock down the HO-1 protein level of the wounds(P＜0.05),promote wound contraction(P＜0.05),reduce the width of the wound(P＜0.01),up-regulate the inflammatory cytokines IL-6 and TNF-α in 3 d,enhance the proliferation of repair cells in wound margin,and improve the growth of the granulation tissue in the R-W-CI model when compared with the conditions after si-NC intervention.Conclusion There exists a sustained high expression level of HO-1 during wound repair,and wound knockdown of HO-1 by siRNA can improve the lack of inflammation status and promote wound healing in R-W-CI mice.