Purpose To investigate the effect and molecu-lar mechanism underlying SOX9 during esophageal squamous cell carcinoma(ESCC)cells.Methods Immunohistochemistry(IHC)was used to detect the expression of SOX9 in ESCC tis-sues and adjacent normal tissues.The correlation of SOX9 ex-pression with clinicopathological features and prognosis of ESCC was analyzed.The differentially expressed genes in Eca109-Vec-tor and Eca109-SOX9 cells were detected by Affymetrix miRNA array.qRT-PCR was used to determine the differential gene in TE-1 and TE-1-siSOX9 cells.The relationship between SOX9 and active/unphosphorylated β-catenin levels was detected by Western blot.The effects of Wnt inhibitor LGK974 on the prolif-eration of ESCC cells were detected by CCK-8.Results SOX9 was highly expressed in ESCC(4.58±3.04)as compared with that in adjacent normal tissues(1.56±2.08,P＜0.001).SOX9 was related to histopathological grade and invasion depth(P＜0.05).Kaplan-Meier analysis indicated high SOX9 expres-sion in ESCC was significantly correlated with shorter overall sur-vival(P＜0.05).Transcriptome profiling and qRT-PCR analysis suggested that SOX9 contributed to the regulation of AXIN2,FZD7 and WNT5A.Overexpression of SOX9 in Eca109 cells in-creased active/unphosphorylated β-catenin levels,whereas silen-cing SOX9 caused a decrease.Significant attenuation of cell pro-liferation was observed at various concentrations of LGK974 with-out affecting SOX9 expression on SOX9-expressing cell lines.As expected,this inhibitory effect was not obvious in Eca109-Vector cells when compared with Eca109-SOX9 cells treated with the same concentration of LGK974.Conclusion SOX9 is highly ex-pressed in ESCC and SOX9-mediated Wnt/β-catenin signal path-way activation at least partially contributes to the SOX9-induced ESCC growth.These findings suggest that SOX9 is a promising prognostic marker and therapeutic target for ESCC.

Viral myocarditis(VMC),as the most common type of myocarditis,can progress to chronic myocarditis and even heart failure,ultimately leading to death.Nuclear factor κB(NF-κB)is a multifunctional transcription factor involved in regulating a wide range of biological processes.Existing evidence suggests that the balance between pro-inflammatory and anti-inflammatory factors plays a decisive role in the prognosis of VMC.The NF-κB pathway mediates inflammatory responses and regulates pathways such as cell apoptosis,energy metabolism,oxidative stress,and insulin resistance to participate in the pathological progression of VMC.This article analyzes and summarizes the molecular mechanism of NF-κB signaling pathway regulation in VMC from the above five aspects,to provide a reference for future basic research and for the clinical diagnosis and treatment of VMC.

Viral myocarditis(VMC),as the most common type of myocarditis,can progress to chronic myocarditis and even heart failure,ultimately leading to death.Nuclear factor κB(NF-κB)is a multifunctional transcription factor involved in regulating a wide range of biological processes.Existing evidence suggests that the balance between pro-inflammatory and anti-inflammatory factors plays a decisive role in the prognosis of VMC.The NF-κB pathway mediates inflammatory responses and regulates pathways such as cell apoptosis,energy metabolism,oxidative stress,and insulin resistance to participate in the pathological progression of VMC.This article analyzes and summarizes the molecular mechanism of NF-κB signaling pathway regulation in VMC from the above five aspects,to provide a reference for future basic research and for the clinical diagnosis and treatment of VMC.

Objective To analyze clinical features of patients with chikungunya fever and provide future reference for prevention and control of the disease. Methods Forty-six confirmed chikungunya fever inpatients were included. Their clinical symptoms, signs, blood count, key biochemical indicators and treatments were analyzed. The comparison between groups were done by ttest. Results The percentages of total cases presenting with fever, rash and joint pain were 100. 0% (46/46), 91. 3% (42/46) and 89. 1% (41/46), respectively. Fifteen (32.6%) cases displayed leucopenia. Increases in lactose dehydrogenase (LDH) and creatine kinase (CK) were observed in 45. 5%(20/44) and 28. 9%(13/45) of the cases, respectively. Three cases displayed an increase of alanine aminotransferase (ALT). Administration of ribavarin extend febrile time compared to symptom-relieving treatments (t=2. 588, P = 0. 013). Conclusions Clinical features of chikungunya fever include fever, rash and joint pain. Good prognosis can be resulted from symptom-relieving treatment. Antiviral treatment may not be beneficial to reducing course of disease.