Safe Hand hygiene practice is considered the most essential component of patient safety. Especially when it comes to the health professionals; students are exposed to their clinical practice in the first years of their education. This accelerates the chances of getting infection via cross transmission of hands through the healthcare worker to the patient is most common.A quantitative cross sectional, questionnaire-based survey was carried out to investigate the level of Knowledge,practices,andattitudeofHandhygieneamonguniversitynursingandoptometryundergraduatestudentsintheirfinalyearoftraining.A total of one hundred and forty three subjects took part in the survey of which 73 nursing students and 70 optometry students. Fisher’s exact test was applied to test the level of Knowledge on practice and attitude. And the correlation coefficient r=-1 to+1 with P>0.05 was obtained. To add, using an observational checklist will be the ideal way to assess the hand hygiene practice in the clinical area. Moreover add a mandatory component as patient safety into the curricula of the undergraduate students of allied health will enhance the patient safety more effectively.