Background: Mongolia ranks first in the world for liver cancer incidence and mortality, with more than 95% of cases associated with hepatitis B virus infection. Healthcare professionals are considered a priority group for vaccination. Aim: To investigate the level of hepatitis B virus immunization among dental specialist physicians. Materials and Methods: This retrospective, observational, analytical cross-sectional study was conducted with approval from the Research Ethics Committee of the Mongolian National University of Medical Sciences (MNUMS). We analyzed serum test data from 76 dentists officially registered in the Dentistry residency program during the 2025–2026 academic year, assessing hepatitis B virus infection status and immunization levels. Statistical analysis of quantitative data was performed using SPSS version 27. Results: Data from 76 dentists were analyzed. The mean age was 28.5±6.2 years; by age group, 70% (n=53) were aged 20–29 years, 22.3% (n=17) were 30–39 years, and 7.8% (n=6) were over 40 years (p<0.05). By sex, 13.2% (n=10) were male (p>0.05). Among the study participants, 97.4% were HBV-negative with a mean age of 28.2±5.3 years (Median: 27.0, IQR: 24.0–31.0), whereas 2.6% were infected; the mean age of infected physicians was 43.5±2.1 years (Median: 43.5, IQR: 42.0–45.0), indicating a high and statistically significant occurrence of HBV infection in the age group over 40 years (Fisher’s exact test, p=0.05). When classified by immunity level, 29.2% (n=19) had anti-HBs levels below 10 mIU/mL (insufficient), 24.6% (n=16) had anti-HBs levels of 10–99 mIU/mL (weak), and 46.2% (n=30) had anti-HBs levels above 100 mIU/mL (adequate immunity); however, no statistically significant association was observed between age groups and protective antibody levels (Fisher’s exact test, p=0.892). Conclusion Among participants, the prevalence of hepatitis B virus infection was 2.6%, vaccination coverage was 85.5%, and anti-HBs antibody titers were adequate in 46.2%.