Objective:To evaluate the role of ZIP7 in sepsis-induced cardiomyopathy in mice.Methods:Ninety wild-type and 90 cardiomyocyte-specific knockout ZIP7 (ZIP7 cKO) male C57BL/6 mice, aged 6-8 weeks, weighing 20-25 g, were divided into 2 groups using a random number table method: wild-type sham operation group (Sham group) and wild-type sepsis group (Sep group), ZIP7 cKO sham operation group (cKO + Sham group) and ZIP7 cKO sepsis group (cKO + Sep group), with 45 mice in each group. The sepsis-induced cardiomyopathy model was developed using the cecal ligation and puncture in anesthetized mice. Twenty mice were randomly selected to record the survival for 10 days postoperatively. At 18 h after surgery, the left ventricular ejection fraction (LVEF) and fractional shortening (LVFS) were measured by echocardiography, and serum concentrations of cardiac troponin T (cTnT), tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were measured by enzyme-linked immunosorbent assay. The contents of hydroxyl radical (·OH) and peroxynitrite anion (ONOO -) were determined using a colorimetric assay, the morphology of myocardial mitochondria was observed with a transmission electron microscope, and the expression of dynamin-related protein 1 (Drp1), mitofusin-2 (Mfn2), and optic atrophy 1 (Opa1) in myocardial tissues was detected using Western blot. Results:Compared to Sham group, the survival rate, LVEF and LVFS were significantly decreased, serum concentrations of cTnT, TNF-α and IL-6 were increased, the contents of ·OH and ONOO - in myocardial tissues were increased, the expression of Drp1 was up-regulated, the expression of Mfn2 and Opa1 was down-regulated ( P<0.05), myocardial cells exhibited mitochondrial swelling, and marked destruction of mitochondrial cristae was observed in Sep group, and no significant differences were found in the aforementioned parameters in cKO+ Sham group ( P>0.05). Compared to Sep group, the survival rate, LVEF and LVFS were significantly increased, serum concentrations of cTnT, TNF-α and IL-6 were decreased, the contents of ·OH and ONOO - in myocardial tissues were decreased, the expression of Drp1 was down-regulated, the expression of Mfn2 and Opa1 was up-regulated ( P<0.05), and mitochondrial swelling in myocardial cells was mild, with less dissolution and destruction of mitochondrial cristae in cKO+ Sep group. Conclusions:Myocardial ZIP7 can promote mitochondrial fusion and inhibit mitochondrial fission, potentially contributing to the mechanism of sepsis-induced cardiomyopathy in mice.

Objective:To evaluate the role of ZIP7 in sepsis-induced cardiomyopathy in mice.Methods:Ninety wild-type and 90 cardiomyocyte-specific knockout ZIP7 (ZIP7 cKO) male C57BL/6 mice, aged 6-8 weeks, weighing 20-25 g, were divided into 2 groups using a random number table method: wild-type sham operation group (Sham group) and wild-type sepsis group (Sep group), ZIP7 cKO sham operation group (cKO + Sham group) and ZIP7 cKO sepsis group (cKO + Sep group), with 45 mice in each group. The sepsis-induced cardiomyopathy model was developed using the cecal ligation and puncture in anesthetized mice. Twenty mice were randomly selected to record the survival for 10 days postoperatively. At 18 h after surgery, the left ventricular ejection fraction (LVEF) and fractional shortening (LVFS) were measured by echocardiography, and serum concentrations of cardiac troponin T (cTnT), tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were measured by enzyme-linked immunosorbent assay. The contents of hydroxyl radical (·OH) and peroxynitrite anion (ONOO -) were determined using a colorimetric assay, the morphology of myocardial mitochondria was observed with a transmission electron microscope, and the expression of dynamin-related protein 1 (Drp1), mitofusin-2 (Mfn2), and optic atrophy 1 (Opa1) in myocardial tissues was detected using Western blot. Results:Compared to Sham group, the survival rate, LVEF and LVFS were significantly decreased, serum concentrations of cTnT, TNF-α and IL-6 were increased, the contents of ·OH and ONOO - in myocardial tissues were increased, the expression of Drp1 was up-regulated, the expression of Mfn2 and Opa1 was down-regulated ( P<0.05), myocardial cells exhibited mitochondrial swelling, and marked destruction of mitochondrial cristae was observed in Sep group, and no significant differences were found in the aforementioned parameters in cKO+ Sham group ( P>0.05). Compared to Sep group, the survival rate, LVEF and LVFS were significantly increased, serum concentrations of cTnT, TNF-α and IL-6 were decreased, the contents of ·OH and ONOO - in myocardial tissues were decreased, the expression of Drp1 was down-regulated, the expression of Mfn2 and Opa1 was up-regulated ( P<0.05), and mitochondrial swelling in myocardial cells was mild, with less dissolution and destruction of mitochondrial cristae in cKO+ Sep group. Conclusions:Myocardial ZIP7 can promote mitochondrial fusion and inhibit mitochondrial fission, potentially contributing to the mechanism of sepsis-induced cardiomyopathy in mice.

The medical contingent of Chinese PLA to libera operated the China Ebola treatment center(ETC) with 100 beds,and employed 80 Liberian health workers according to the task requirements and the normal operation demand.Under consideration of current health education situation and epidemic prevention requirements,combined with experiences in the fight against SARS,the training content includes the Ebola prevention and control knowledge,isolation of patients and safe burial,malaria prevention and control knowledge,hospital disinfection technology,psychological intervention.Training methods diversified,with theoretical teaching,site visit,on-site demonstration and training and video guide.With the pass of the simulation training evaluation model in all staff,the expected goals of pre-service training have been achieved.

Objective To investigate the quality of life(QOL)of patients with stroke and its related factors.Methods The QOL of eighty-two stroke survivors was assessed in terms of SF-36 scores,motor impairment (Fugl-Meyer),disability[Barthel index(BI)]and depression(HAMD)more than one year after their first episode.Their social demographic characteristics such as age,gender,education and income were recorded,as were the characteristics of their disease and any complications.Multiple regression analysis was used to analyse the related factors.Results The average age of the 82 subjects was 61.89 + 9.81 years(range,33-81).56 of them were male (68.2％).Scores on all items of the SF-36 for these subjects were lower than those of a normal population.Correlation analysis showed income,cost for medical care,regular exercise,movement function,ability in the activities of daily living,depression,hypertension and shoulder pain were significantly correlated with most of the SF-36 domain scores.Motor function,income and cost for medical care explained 34％ of the variance in SF-36 scores.Motor function was the single most important factor.Conclusion The major factors affecting the QOL of stroke patients are motor function,level of income and cost for medical care.Paying more attention to rehabilitation treatment,taking regular exercise and more income could help improve the QOL of stroke survivors.