Impacted third molar extractions are one of the most common oral surgical procedures. In cases where a pathological condition is observed, it is relatively easy to decide on surgery. However, cystic changes can be observed in follicular tissues even in cases where pathological changes are not observed on radiography. The present study aimed to evaluate the histopathologic changes in dental follicles associated with radiographically normal impacted lower third molar. One hundred and one dental follicles were obtained after third molar surgeries. The patients with follicular width of less than 2.5 mm in the panoramic radiographs were included in the study. The relationships between pathological changes in follicular tissues and angular position of impacted teeth, age and gender were statistically examined. Cystic changes were observed in 65 (64.3%) of 101 follicles examined. The relationship between cystic changes and the angular position of impacted lower third molars was statistically significant (p < 0.05). Cystic changes were observed in 13 (43.3%) of 30 mesioangular teeth, 24 (80%) of 30 vertical teeth, 19 (65.5%) of 29 horizontal teeth, and 9 (75%) of 12 distoangular teeth. About 60% of cystic changes and 64.06% of inflammation occurred in patients between the ages of 20 and 30. Cystic changes can be observed in the follicular tissues of impacted lower third molars that are radiographically normal. There is a probability of histopathological changes, especially in patients over the age of 20 years and in the impacted lower third molars in the vertical position.

Objective: To investigate the in vitro antimicrobial potential of Thermopsis turcica Kit Tan, Vural &Kü?ük?dük against periodontopathogenic bacteria, its antioxidant activity and cytotoxic effect on various cancer cell lines.Methods: In vitro antimicrobial activities of ethanol, methanol, ethyl acetate (EtAc), n-hexane and water extracts of Thermopsis turcica herb against periodontopathogenic bacteria, Aggregatibacter actinomycetemcomitans ATCC 29523 and Porphyromonas gingivalis ATCC 33277 were tested by agar well diffusion, minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Antioxidant properties of the extracts were evaluated by 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity and β-carotene bleaching methods. Amounts of phenolic contents of the extracts were also analysed by using the Folin-Ciocalteu reagent. Additionally, cytotoxic activity of the extracts on androgen-insensitive prostate cancer, androgen-sensitive prostate cancer, chronic myelogenous leukemia and acute promyelocytic leukemia human cancer cell lines were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Human gingival fibroblast cells were used as a control.Results:Our data showed that EtAc extract had the highest antimicrobial effect on Aggregatibacter actinomycetemcomitans (MIC: 1.562 mg/mL, MBC: 3.124 mg/mL) and Porphyromonas gingivalis (MIC: 0.781 mg/mL, MBC: 1.562 mg/mL). In antioxidant assays, EtAc extract exhibited also the highest radical scavenging activity [IC50=(30.0±0.3) μg/mL] and the highest inhibition [(74.35±0.30)%] against lineloic acide oxidation. The amount of phenolic content of it was also the highest [(162.5±1.2) μg/mg gallic acid]. In cytotoxic assay, only ethanol [IC50=(80.00±1.21) μg/mL] and EtAc extract [IC50=(70.0±0.9) μg/mL] were toxic on acute promyelocytic leukemia cells at 20-100 μg/mL (P<0.05). However, no toxic effect was observed on human gingival fibroblast cells.Conclusions:According to our findings, owing to its antioxidant and cytotoxic potential, EtAc extract might include anticancer agents for acute promyelocytic leukemia.