OBJECTIVE To explore the medication rule and potential mechanism of anti-aging traditional Chinese medicine health care prescription by using data mining,network pharmacology and molecular docking technology.METHODS By searching the special food information query platform of the State Administration for Market Regulation and Yaozhi.com,the health care prescrip-tions with traditional Chinese medicine as the main component and anti-aging effect were searched.The data mining technology was used to analyze the prescription rules,and the core anti-aging traditional Chinese medicine was selected according to the analysis re-sults.The chemical constituents and corresponding targets of core anti-aging traditional Chinese medicines were obtained by searching TCMSP and other databases;potential targets related to aging from GeneCards and OMIM databases were screened;the intersection tar-gets of core Chinese medicine and aging were obtained.Subsequently,protein-protein interaction(PPI)network analysis,gene ontolo-gy(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed using STRING database and Cytoscape 3.7.2 software.Finally,the affinity of core targets and key components was verified by molecular docking technology.RESULTS A total of 244 kinds of anti-aging traditional Chinese medicine health care prescriptions were screened and included.The prescription drugs were mainly tonic drugs,the drug properties were mostly warm and mild,the drug taste was mainly sweet,the drugs were mainly distributed in the five zang organs,with the kidney meridian being the first target.According to the re-sults of comprehensive data analysis,five core Chinese medicines(Wolfberry,ginseng,American ginseng,Astragalus,Poria)were screened out.A total of 105 active ingredients(corresponding to 219 target genes),1 568 aging-related potential targets,and 137 in-tersection targets were obtained.According to the degree value,12 core targets were screened out,mainly involving cancer pathways,lipids and atherosclerosis,AGE-RAGE and other signaling pathways.The results of molecular docking showed that the key anti-aging components could spontaneously and stably bind to the core targets.CONCLUSION Through data mining,network pharmacology and molecular docking,the prescription rules of anti-aging Chinese medicine health care prescriptions are analyzed,and the anti-ag-ing potential targets and mechanism pathways of five core traditional Chinese medicines are preliminarily revealed,so as to provide ref-erence and theoretical basis for delaying aging and preventing and treating related diseases in the elderly.

OBJECTIVE To explore the medication rule and potential mechanism of anti-aging traditional Chinese medicine health care prescription by using data mining,network pharmacology and molecular docking technology.METHODS By searching the special food information query platform of the State Administration for Market Regulation and Yaozhi.com,the health care prescrip-tions with traditional Chinese medicine as the main component and anti-aging effect were searched.The data mining technology was used to analyze the prescription rules,and the core anti-aging traditional Chinese medicine was selected according to the analysis re-sults.The chemical constituents and corresponding targets of core anti-aging traditional Chinese medicines were obtained by searching TCMSP and other databases;potential targets related to aging from GeneCards and OMIM databases were screened;the intersection tar-gets of core Chinese medicine and aging were obtained.Subsequently,protein-protein interaction(PPI)network analysis,gene ontolo-gy(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed using STRING database and Cytoscape 3.7.2 software.Finally,the affinity of core targets and key components was verified by molecular docking technology.RESULTS A total of 244 kinds of anti-aging traditional Chinese medicine health care prescriptions were screened and included.The prescription drugs were mainly tonic drugs,the drug properties were mostly warm and mild,the drug taste was mainly sweet,the drugs were mainly distributed in the five zang organs,with the kidney meridian being the first target.According to the re-sults of comprehensive data analysis,five core Chinese medicines(Wolfberry,ginseng,American ginseng,Astragalus,Poria)were screened out.A total of 105 active ingredients(corresponding to 219 target genes),1 568 aging-related potential targets,and 137 in-tersection targets were obtained.According to the degree value,12 core targets were screened out,mainly involving cancer pathways,lipids and atherosclerosis,AGE-RAGE and other signaling pathways.The results of molecular docking showed that the key anti-aging components could spontaneously and stably bind to the core targets.CONCLUSION Through data mining,network pharmacology and molecular docking,the prescription rules of anti-aging Chinese medicine health care prescriptions are analyzed,and the anti-ag-ing potential targets and mechanism pathways of five core traditional Chinese medicines are preliminarily revealed,so as to provide ref-erence and theoretical basis for delaying aging and preventing and treating related diseases in the elderly.

OBJECTIVE:To evaluate the efficacy and safety of Yupingfeng powder combined with second- generation antihistamines versus second-generation antihistamines for chronic urticaria(CU)systematically,and to provide evidence-based reference for clinical treatment for CU. METHODS:Retrieved from PubMed,Embase,The Cochrane Library,CJFD,VIP and CBM,RCT about therapeutic efficacy(total response rate,cure rate,recurrence rate)and safety(the incidence of ADR)of Yupingfeng combined with second-generation antihistamines(trial group)versus second-generation antihistamines(control group) in the treatment of CU were collected. The data extraction was performed for included clinical studies,and Meta-analysis was performed by using Rev Man 5.3 statistical software after quality evaluation with Cochrane Handbook 5.1.0 evaluation criteria. RESULTS:A total of 34 RCTs were enrolled,involved 3 405 patients in total. Results of Meta-analysis showed that the total response rate [OR=4.02,95%CI(3.03,5.34),P<0.001],cure rate [OR=2.25,95%CI(1.95,2.60),P<0.001] and recurrence rate [OR=0.33,95%CI(0.26,0.42),P<0.001] of trial group were significantly better than those of control group,with statistical significance. There was no statistical significance in the incidence of ADR between 2 groups [OR=0.98,95%CI(0.71,1.37),P=0.92]. CONCLUSIONS:For CU therapy,Yupingfeng powder combined with second-generation antihistamines is better than second-generation antihistamines alone in improving total response rate and cure rate,reducing recurrence rate,both have similar safety.

Objective To study the effect of sevoflurane pretreatment or aftertreatment on intrapulmonary shunt for patients with one lung ventilation(OLV)during thoracic surgical procedures .Methods 80 ASA I or II patients underwent thoracic surgery with OLV were randomly divided into four groups(n= 20 for each):(1)Propofol group(group P);(2)Sevoflurane pretreatment group(group S1);(3)Sevoflurane aftertreatment group(group S2);(3)Sevoflurane whole-treatment group(group S3) .Blood sam-ples were taken from radial artery and right atrial blood before induction of anesthesia(baseline T .0) ,before OLV(sevoflurane pre-treatment 30 min ,T1) ,after OLV 30 ,60 min and before the end of OLV(T2-T4)and after the end of OLV(T5)for measurement of blood gases and calculation of Qs/Qt .The indexs of hemodynamics and relative clinical data were recorded .Results Arterial oxy-gen pressure of the four groups for OLV significantly reduced ,when compared with double lung ventilation(P<0 .05) ,but there was no significant difference among the four groups(P>0 .05) .Intrapulmonary shunt of the four groups for OLV significantly in-creased ,when compared with double lung ventilation(P<0 .05) ,but intrapulmonary shunt of S1 ,S2 and S3 groups significantly de-creased ,when compared with P group ,and there was no significant difference for S1 ,S2 and S3 groups(P> 0 .05) .Conclusion Sevoflurane can decrease intrapulmonary shunt for patients during OLV ,but there is no significant difference for pretreatment or af-tertreatment or whole treatment and do not increase arterial oxygen pressure .

Porcine reproductive and respiratory syndrome virus (PRRSV) has been recognized as one of the most important pathogens of pigs throughout the world. In 2006, more than 10 provinces of China have experienced an epizootic outbreak of pig diseases characterized by high fever, reddened skin and high morbidity and mortality. From June 2006 to April 2007, we have investigated some clinical samples in Hubei province by RT-PCR and cloned several major genes, N, GP5 and NSP2 gene, shown in this study. Phylogenetic analysis of these genes revealed that the highly pathogenic PRRSV variant, ZB, was responsible for 2006 emergent outbreak of pig disease in Hubei province similar with those variants isolated from other provinces in China in 2006, and belongs to the NA-type PRRSV. In the PRRSV variants, the N and GP5 shear about 90% identity with prototypic ATCC VR-2332 and some typical NA-type Chinese isolates, except the 2850bp NSP2 gene (only shares 65% identity with ATCC VR-2332). But they all shear more than and 97% identity with other highly pathogenetic Chinese PRRSV strains. Additionally, there are extensive amino acid (aa) mutations in the GP5 protein and 2 deletions in the Nsp2 protein when compared with the previous isolates. Most of the variants found in 2006 epizootic outbreak of pig diseases in China were the farthest variants from the typical NA-type PRRSV in phylogenetic distance, and these diversities may be responsible for the differences in the pathogenicity observed between these variants and original Chinese PRRSV strains.