Background:Ribosomal protein L22-like 1(RPL22L1)exerts regulatory effects on various malignant tumors such as lung cancer,prostate cancer,and cervical cancer.However,its role in colorectal cancer(CRC)remains unclear.Aims:To investigate the expression of RPL22L1 in CRC and its role in patients' prognosis and glucose metabolism of tumor cells.Methods:A total of 142 newly diagnosed CRC patients admitted to the Taizhou First People's Hospital from February 2022 to June 2024 were enrolled.The expression levels of RPL22L1 mRNA and protein were detected by quantitative real-time PCR and immunohistochemistry,respectively.The correlation between RPL22L1 expression and clinicopathological characteristics was analyzed.Kaplan-Meier survival analysis was used to evaluate the impact of RPL22L1 expression on the prognosis of CRC patients.RPL22L1 siRNA was transfected into SW480 cells to establish a low-expression cell model.Cell proliferation was assessed by CCK-8 assay,cell migration by Transwell chamber assay,and apoptosis by flow cytometry.Gene set enrichment analysis(GSEA)was performed to evaluate the effect of RPL22L1 on glucose metabolism of tumor cells.Results:The expression levels of RPL22L1 mRNA and protein were significantly higher in CRC tissues than in adjacent normal tissues(all P<0.05).The expression level of RPL22L1 mRNA was correlated with the TNM stage and carcinoembryonic antigen level of CRC(all P<0.05).Kaplan-Meier analysis showed that the cumulative survival rate of high RPL22L1 mRNA expression group was significantly lower than that of low-expression group(P=0.027).The expression level of RPL22L1 mRNA was significantly higher in SW480 cells than in normal intestinal epithelial cells(P<0.001).After inhibiting RPL22L1 expression,the proliferation and migration capacities of SW480 cells were significantly decreased(all P<0.05),the apoptosis rate was significantly increased(P=0.005),and the lactate level and relative glucose uptake level were significantly reduced(all P<0.05).GSEA indicated that RPL22L1 gene was associated with glycolysis/gluconeo-genesis(P=0.02).Conclusions:RPL22L1 is highly expressed in CRC and is associated with poor prognosis of patients,suggesting its potential as a molecular target for CRC therapy.Furthermore,RPL22L1 may promote the tumorigenesis and progression of CRC by modulating glucose metabolism.

Background:Ribosomal protein L22-like 1(RPL22L1)exerts regulatory effects on various malignant tumors such as lung cancer,prostate cancer,and cervical cancer.However,its role in colorectal cancer(CRC)remains unclear.Aims:To investigate the expression of RPL22L1 in CRC and its role in patients' prognosis and glucose metabolism of tumor cells.Methods:A total of 142 newly diagnosed CRC patients admitted to the Taizhou First People's Hospital from February 2022 to June 2024 were enrolled.The expression levels of RPL22L1 mRNA and protein were detected by quantitative real-time PCR and immunohistochemistry,respectively.The correlation between RPL22L1 expression and clinicopathological characteristics was analyzed.Kaplan-Meier survival analysis was used to evaluate the impact of RPL22L1 expression on the prognosis of CRC patients.RPL22L1 siRNA was transfected into SW480 cells to establish a low-expression cell model.Cell proliferation was assessed by CCK-8 assay,cell migration by Transwell chamber assay,and apoptosis by flow cytometry.Gene set enrichment analysis(GSEA)was performed to evaluate the effect of RPL22L1 on glucose metabolism of tumor cells.Results:The expression levels of RPL22L1 mRNA and protein were significantly higher in CRC tissues than in adjacent normal tissues(all P<0.05).The expression level of RPL22L1 mRNA was correlated with the TNM stage and carcinoembryonic antigen level of CRC(all P<0.05).Kaplan-Meier analysis showed that the cumulative survival rate of high RPL22L1 mRNA expression group was significantly lower than that of low-expression group(P=0.027).The expression level of RPL22L1 mRNA was significantly higher in SW480 cells than in normal intestinal epithelial cells(P<0.001).After inhibiting RPL22L1 expression,the proliferation and migration capacities of SW480 cells were significantly decreased(all P<0.05),the apoptosis rate was significantly increased(P=0.005),and the lactate level and relative glucose uptake level were significantly reduced(all P<0.05).GSEA indicated that RPL22L1 gene was associated with glycolysis/gluconeo-genesis(P=0.02).Conclusions:RPL22L1 is highly expressed in CRC and is associated with poor prognosis of patients,suggesting its potential as a molecular target for CRC therapy.Furthermore,RPL22L1 may promote the tumorigenesis and progression of CRC by modulating glucose metabolism.

Objective To retrieve,evaluate and summarise the relevant evidence in non-pharmacological management of venous thromboembolism in patients with haemorrhagic stroke during perioperative period so as to provide references for clinical practice. Methods According to the "6S" evidence pyramid mode,evidence was retrieved from top to bottom across databases such as BMJ Best Practice,UpToDate,JBI Evidence-Based Healthcare Centre,the National Guideline Clearinghouse (NGC),the National Institute for Health and Care Excellence (NICE),the Scottish Intercollegiate Guidelines Network (SIGN),the Registered Nurses' Association of Ontario (RNAO) guidelines network,the Canadian Heart and Stroke Foundation (HSF),the American Heart Association/American Stroke Association (AHA/ASA),the Neuro-Critical Care Society (NCS),the European Stroke Organization (ESO),Cochrane Library,PubMed,Medline,CNKI,Web of Science,Embase,the China Biomedical Literature Database,Wanfang Data,Medlive,VIP,and the Chinese Medical Journal Full-Text Database. The search focused on non-pharmacological management of perioperative venous thromboembolism in haemorrhagic stroke patients,including guidelines for clinical practice,expert consensus,systematic reviews and evidence summaries. The searched literatrue was from the inception of the databases to April 11th,2024. Two researchers independently evaluated the quality of the literature,extracted,integrated and summarised the best evidences. Results A total of 23 articles were included,comprising 12 guidelines,5 systematic reviews,4 expert consensuses and 2 evidence summaries. Thirty-one pieces of the best evidence were summarised and integrated into 5 topics,including multidisciplinary teamwork,risk assessment and screening,basic prevention,mechanical prophylaxis and health education. Conclusion This study has summarised the best evidence for non-pharmacological management of venous thromboembolism in patients with haemorrhagic stroke during the perioperative period. It provides evidence-based references for clinical medical staff to prevent and manage venous thromboembolism in patients with hemorrhagic stroke during perioperative period.

Objective:To explore the gender differences in body mass index (BMI), behavioral symptoms, and psychological characteristics in patients with eating disorders (ED).Methods:A total of 986 ED patients, including 54 males and 932 females, who visited the Peking University Sixth Hospital from August 2008 to November 2015 were included in the study. A cross-sectional survey of ED-related clinical manifestations was conducted using the Eating Disorder Questionnaire (EDQ) and the Eating Disorder Inventory-1 (EDI-1). A 1∶4 propensity score matching was utilized to eliminate the potential effects of age and diagnostic category on the symptom presentation of ED patients. To further validate gender differences in symptoms, multiple linear regression was conducted. T-tests, rank sum tests, and chi-square tests were used to compare EDQ item data and EDI-1 scores between male and female ED patients. Network analysis was employed to identify the most critical symptoms of ED patients and compare them between genders.Results:After matching, 54 male and 213 female patients were included in the analysis. There were no statistically significant differences in age ( t=-0.02) and diagnostic composition (χ 2=0.28) between the groups (all P>0.05). The difference between desired and current BMI was significantly different between genders (1.3(-0.6, 3.2) kg/m 2 for males vs.-0.3(-2.3, 2.4) kg/m 2 for females; Z=-2.64, P<0.01), with males desiring a higher weight and females a lower weight. Fewer male ED patients chose to reduce staple food intake as a dieting method compared to females (41.5% (17/41) vs. 63.2% (117/185); χ 2=6.60, P=0.010). Males had lower scores than females for Drive for Thinness and Interoceptive Awareness ( Z=-3.19, -2.26, respectively; all P<0.05). After controlling for demographic variables and other factor scores, gender was found to significantly influence the Drive for Thinness factor score (β=3.10, P<0.001). Network analysis revealed that the core nodes for both male and female ED patients were Drive for Thinness and Interoceptive Awareness, consistent with the entire sample. Conclusions:Male ED patients exhibited a weaker motivation for thinness compared to female patients. In the symptom network analysis, Drive for Thinness and Interoceptive Awareness were identified as the most significant symptoms for both genders.

Objective To retrieve,evaluate and summarise the relevant evidence in non-pharmacological management of venous thromboembolism in patients with haemorrhagic stroke during perioperative period so as to provide references for clinical practice. Methods According to the "6S" evidence pyramid mode,evidence was retrieved from top to bottom across databases such as BMJ Best Practice,UpToDate,JBI Evidence-Based Healthcare Centre,the National Guideline Clearinghouse (NGC),the National Institute for Health and Care Excellence (NICE),the Scottish Intercollegiate Guidelines Network (SIGN),the Registered Nurses' Association of Ontario (RNAO) guidelines network,the Canadian Heart and Stroke Foundation (HSF),the American Heart Association/American Stroke Association (AHA/ASA),the Neuro-Critical Care Society (NCS),the European Stroke Organization (ESO),Cochrane Library,PubMed,Medline,CNKI,Web of Science,Embase,the China Biomedical Literature Database,Wanfang Data,Medlive,VIP,and the Chinese Medical Journal Full-Text Database. The search focused on non-pharmacological management of perioperative venous thromboembolism in haemorrhagic stroke patients,including guidelines for clinical practice,expert consensus,systematic reviews and evidence summaries. The searched literatrue was from the inception of the databases to April 11th,2024. Two researchers independently evaluated the quality of the literature,extracted,integrated and summarised the best evidences. Results A total of 23 articles were included,comprising 12 guidelines,5 systematic reviews,4 expert consensuses and 2 evidence summaries. Thirty-one pieces of the best evidence were summarised and integrated into 5 topics,including multidisciplinary teamwork,risk assessment and screening,basic prevention,mechanical prophylaxis and health education. Conclusion This study has summarised the best evidence for non-pharmacological management of venous thromboembolism in patients with haemorrhagic stroke during the perioperative period. It provides evidence-based references for clinical medical staff to prevent and manage venous thromboembolism in patients with hemorrhagic stroke during perioperative period.

Objective:To explore the gender differences in body mass index (BMI), behavioral symptoms, and psychological characteristics in patients with eating disorders (ED).Methods:A total of 986 ED patients, including 54 males and 932 females, who visited the Peking University Sixth Hospital from August 2008 to November 2015 were included in the study. A cross-sectional survey of ED-related clinical manifestations was conducted using the Eating Disorder Questionnaire (EDQ) and the Eating Disorder Inventory-1 (EDI-1). A 1∶4 propensity score matching was utilized to eliminate the potential effects of age and diagnostic category on the symptom presentation of ED patients. To further validate gender differences in symptoms, multiple linear regression was conducted. T-tests, rank sum tests, and chi-square tests were used to compare EDQ item data and EDI-1 scores between male and female ED patients. Network analysis was employed to identify the most critical symptoms of ED patients and compare them between genders.Results:After matching, 54 male and 213 female patients were included in the analysis. There were no statistically significant differences in age ( t=-0.02) and diagnostic composition (χ 2=0.28) between the groups (all P>0.05). The difference between desired and current BMI was significantly different between genders (1.3(-0.6, 3.2) kg/m 2 for males vs.-0.3(-2.3, 2.4) kg/m 2 for females; Z=-2.64, P<0.01), with males desiring a higher weight and females a lower weight. Fewer male ED patients chose to reduce staple food intake as a dieting method compared to females (41.5% (17/41) vs. 63.2% (117/185); χ 2=6.60, P=0.010). Males had lower scores than females for Drive for Thinness and Interoceptive Awareness ( Z=-3.19, -2.26, respectively; all P<0.05). After controlling for demographic variables and other factor scores, gender was found to significantly influence the Drive for Thinness factor score (β=3.10, P<0.001). Network analysis revealed that the core nodes for both male and female ED patients were Drive for Thinness and Interoceptive Awareness, consistent with the entire sample. Conclusions:Male ED patients exhibited a weaker motivation for thinness compared to female patients. In the symptom network analysis, Drive for Thinness and Interoceptive Awareness were identified as the most significant symptoms for both genders.

Objective : To compare and analyze the effects of thermal etching on the shear strength of zirconia substrates and decorative ceramics. Methods: A total of 20 specimens made with zirconia ceramics were randomly divided into an observation group and a control group with 10 cases in each group. The control group was treated with sandblasting, while the observation group was treated with sandblasting and thermal etching. The surface characteristics were examined by scanning electron microscope (SEM) and phase analysis of X-ray diffraction (XRD), and the shear strength was tested using a universal testing machine. The characteristics of surface destruction were examined by SEM. Results : SEM showed that the peak structure was observed in both groups. The observation group exhibited deep fissures, and the control group exhibited small fissures. The diffraction peaks of the two groups are similar. The T (101) peak is the main peak, and both groups exhibit an M (111) peak. However, the peak intensity is relatively small. The relative levels of monoclinic zirconia were 15.16% in the observation group and 16.22% in the control group. The shear bond strength of the observation group was 24.74 ± 3.02 MPa, which was significantly higher than that of the control group at 21.09 ± 2.58 MPa. The difference was statistically significant (t=2.599, P=0.021). In the control group, the porcelain residue on the zirconia surface was minimal at low magnification, and the zirconia substrate was obviously exposed. The zirconia surface was similar to cristae obliqua at high magnification, and the porcelain exhibited a scattered distribution. In the observation group, a large amount of residual veneer porcelain remained on the zirconia surface at low magnification, but considerable porcelain was observed at high magnification. Conclusion Thermal etching and sandblasting treatment can improve the shear strength of zirconia substrate.

Objective : To compare the clinical effects of polymer ceramic onlay and all ceramic crown in short crown molars Methods : 45 cases of polymer ceramic onlay and 45 cases of Zirconium dioxide all ceramic crowns which repaired the short crowm molars after root canal therapy were compared clinically from the aspects of beauty of restoration, fracture and dislodgement, marginal adaptation and the preservation of abutment teeth. Results : One polymer ceramic onlay had fall out form the teeth after half year, while the all ceramic crowns have been 8 cases after 6~12 months. There were significant differences in fracture and dislodgement between the two groups (χ2=4.44, P < 0.05). Conclusion Polymer ceramic onlay has good clinical effects in the restoration of short crown molars.

Objective To discuss the value of multi-slice spiral CT angiography (MSCTA)and digital subtraction angiography (DSA)in lower extremity arteriosclerosis obliterans.Methods MSCTA and DSA of lower extremity arteries were performed in 41 patients with lower extremity arteriosclerosis obliterans sequentially.The interval of these two examinations was no more than 2 weeks.DSA served as the reference standard and compared with MSCTA in terms of grading of stenoocclusive lesions,visualization of collaterals in lower extremity arteries.Results No significant differences of grading of stenoocclusive lesions,visualization of col-laterals were observed between MCTA and DSA in lower extremity arteries.Compared with the results of DSA,the sensitivity,spe-cificity,accuracy,positive and negative predictive value of MSCTA in the detection of arterial stenosis (≥ 50%)were 97.2%, 98.2%,93.8%,94.6%,98.2%,respectively.Conclusion MSCTA shows relatively high sensitivity and specificity in the diagnosis of lower extremity arteriosclerosis obliterans,can provide effective guidance of planning treatment in lower extremity arteriosclerosis obliterans.

To select appropriate descriptors for response of the patient-reported outcome (PRO) scale for the main symptoms of patients with chronic obstructive pulmonary disease (COPD) complicated with pulmonary heart disease.